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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2006

2005

Abstract

Seeds of Abies lasiocarpa (Hook.) Nutt. (subalpine fir) were dissected, and the different parts were analyzed for elemental composition. The data were used to design a novel growth medium for initiation of somatic embryogenesis. Embryogenic cultures were initiated from immature zygotic embryos from six open-pollinated families of A. lasiocarpa on three different media.The frequency of initiation was the highest in early to mid-July when the zygotic embryo explants were ca. 0.8 mm long. Thereafter the response declined rapidly.The culture media did not significantly affect the initiation frequencies, but the subsequent growth and culture survival was dependent on the culture medium. On the Schenk Hildebrandt medium, many cultures ceased to grow and died. Several of the decaying cultures were rescued after transfer to one of the new media. Proliferating cultures could be stimulated to produce mature embryos. Of 2510 mature somatic embryos, 212 (8.4%) converted to plants, and 35 plants have grown over two periods.

Abstract

Stilbene synthases make the backbone of stilbenes in a single enzymatic step. Many stilbenes are stressinduced antimicrobial phenolics, believed to work in disease resistance. In conifers, stilbenes are found in pine (Pinus), spruce (Picea) and a few other genera.Stilbene synthase isoforms in pine use cinnamyl-CoA to form pinosylvin, these are termed pinosylvin synthases, whereas stilbene synthases in spruce use pcoumaryl- CoA to form resveratrol and are sometimes termed resveratrol synthases.Pinosylvin has been found to be more effective than resveratrol in inhibiting fungal growth and wood decay (Seppnen et al. 2004), and pathogens of non-pinosylvin producing species have been found to be less tolerant of pinosylvin than pine pathogens (Seppnen et al. 2004). In the present study, Norway spruce (Transformation of Norway spruce with the pinosylvin synthase gene, PSS1) was transformed using the biolistic technique with a gene encoding pinosylvin synthase, PSS1, from Scots pine and the E. coli nptII antibiotic resistance gene.Vector constructs carrying PSS1 in sense and antisense, as well as control vectors without PSS1 were transferred into two embryogenic cell lines of Norway spruce, 11703-B63 and 186-3C. Selection condition for transgenic tissue was conferred by nptII in combination with the antibiotic geneticin. Geneticin resistant lines were recovered from all transformation events, a total of 55 lines.NptII was detected by PCR analysis in many of these lines, the majority derived from the cell line 11703 B63. However, nptII protein was detected in just five lines, and several lines of evidence indicate that the transgenic lines obtained in this study might be chimaeras.Fifty-six seedlings were successfully regenerated from antibiotic resistant lines, 50 of these were derived from cell line 11703 B63. All seedlings died during cold storage before further testing could be carried out.

2003

Abstract

Efforts to improve efficiencies in the forest industries have mainly focused on problems within the borders of the company. Thus, there is an unexploited potential for increased efficiency through integrating and coordinating activities between companies. This poster presents games that mimic the forest industry and demonstrate to...

2002

Abstract

Six experimental sites in the coastal spruce (Picea abies L. Karst.) forest of central Norway were investigated. A comparison of different silviculture systems was performed based on the present situation of mature stands, and a single tree selection system, a group system and a clearcutting system were studied. The diameter distribution shows the classical inverse J-shaped curve at five of six sites. The mean tree volume removed in the selection system was 0.6 m3, and about 0.3 m3 for the other two methods. The harvesting cost was about 14 Euro/m3 for clearcutting, and about 10% higher for the group and selection systems. In simulations of all future revenues and expenses based on the observed stand conditions, cutting and extraction costs, the net present value (NPV) was app. 15% lower for the group and selection systems compared to clearcutting. A lower stem volume production in uneven-aged stands and higher administration and harvesting costs had only a minor influence on the NPV. One percent higher interest rate lowered the NPV of the group and selection systems to 75% of the clearcutting method.

Abstract

Two games, called “Wood Supply Games”, are developed based on the structure and dynamics of the Beer Game. The games are intended as student exercises in forestry logistics courses. By introducing divergent and convergent flows in the supply chain, the relevance to the forest sector is increased. Results from pilot experiments of the games are presented and discussed in terms of how different degrees of complexity in supply chain structures may affect their efficiency. Measures of efficiency are accumulated costs, amplification of demand and the sample variance of order rates. Results indicate that supply chain performance is negatively affected by increasing rigidity of constraints at points of divergence. Furthermore, lower degrees of efficiency and predictability are observed as complexity of supply chain structure increases. Testing of results and proposed hypotheses can be accomplished by running numerous replications of the games, or alternatively, by development of appropriate simulation models.

2000

Abstract

The influence of light quality on the proliferation of embryogenic tissue of three genotypes of Norway spruce (Picea abies [L.] Karst), with different capacities for mature somatic embryo production, was studied.The proliferating tissues were subjected to light from commercially available light sources: Philips TLD Warm White 36W/29, Philips TLD Blue 18W/18, Philips TLD Red 36W/15, Osram L Fluora 36W/77 and Sylvania Far Red 7080, for 18 h a day with the photon flux (PAR) at 30 mol m-2 s-1.The effect of light quality on the growth of embryogenic tissue was strongly genotype dependent. In genotype 164-4 tissue proliferation was strongly inhibited by blue and red light. Genotype 86:52 reacted in a similar way, but not as strongly as 164-4, whereas the tissue of genotype 186-3 was almost insensitive to light quality and grew fast in all light conditions.