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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2019

Abstract

Invasive alien species and new plant pests are introduced into new regions at an accelerating rate, due to increasing international trade with soil, plants and plant products. Exotic, plant pathogenic oomycetes in soil from the root zone of imported plants pose a great threat to endemic ecosystems and horticultural production. Detecting them via baiting and isolation, with subsequent identification of the isolated cultures by Sanger sequencing, is labour intensive and may introduce bias due to the selective baiting process. We used metabarcoding to detect and identify oomycetes present in soil samples from imported plants from six different countries. We compared metabarcoding directly from soil both before and after baiting to a traditional approach using Sanger-based barcoding of cultures after baiting. For this, we developed a standardized analysis workflow for Illumina paired-end oomycete ITS metabarcodes that is applicable to future surveillance efforts. In total, 73 soil samples from the rhizosphere of woody plants from 33 genera, in addition to three samples from transport debris, were analysed by metabarcoding the ITS1 region with primers optimized for oomycetes. We detected various Phytophthora and Pythium species, with Pythium spp. being highly abundant in all samples. We also found that the baiting procedure, which included submerging the soil samples in water, resulted in the enrichment of organisms other than oomycetes, compared to non-baited soil samples.

2018

Abstract

Increasing abundance of Juncus effusus (soft rush) and Juncus conglomeratus (compact rush) in pastures and meadows in western Norway has caused reductions in forage yield and quality in recent decades. Understanding plant development and regrowth following cutting is essential in devising cost-effective means to control rushes. In a field experiment in western Norway, we investigated development of above- and below-ground fractions of rush from seedlings to three-year-old plants, including the impact on vigour of disturbing growth by different cutting frequencies during the period 2009–2012. Each year, the plants were exposed to one or two annual cuts or left untreated and five destructive samplings were performed from March to early December. Juncus effusus showed significantly more vigorous growth than Juncus conglomeratus in the last two years of the study period. The above-ground:below-ground biomass ratio of both species increased mainly in spring and early summer and was reduced in late summer and autumn. Removal of aerial shoots also reduced the below-ground fraction of both species. One annual cut in July effectively reduced biomass production in both species by 30–82%, which was only a slightly smaller reduction than with two annual cuts, in June and August. Mechanical control measures such as cutting can thus effectively reduce rush vigour when performed late in the growing season.