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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2023

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Abstract

Stress can have long-lasting impacts on plants. Here we report the long-term effects of the stress hormone jasmonic acid (JA) on the defence phenotype, transcriptome and DNA methylome of Arabidopsis. Three weeks after transient JA signalling, 5-week-old plants retained induced resistance (IR) against herbivory but showed increased susceptibility to pathogens. Transcriptome analysis revealed long-term priming and/or upregulation of JA-dependent defence genes but repression of ethylene- and salicylic acid-dependent genes. Long-term JA-IR was associated with shifts in glucosinolate composition and required MYC2/3/4 transcription factors, RNA-directed DNA methylation, the DNA demethylase ROS1 and the small RNA (sRNA)-binding protein AGO1. Although methylome analysis did not reveal consistent changes in DNA methylation near MYC2/3/4-controlled genes, JA-treated plants were specifically enriched with hypomethylated ATREP2 transposable elements (TEs). Epigenomic characterization of mutants and transgenic lines revealed that ATREP2 TEs are regulated by RdDM and ROS1 and produce 21 nt sRNAs that bind to nuclear AGO1. Since ATREP2 TEs are enriched with sequences from IR-related defence genes, our results suggest that AGO1-associated sRNAs from hypomethylated ATREP2 TEs trans-regulate long-lasting memory of JA-dependent immunity.

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Abstract

The accumulation of petroleum-based plastics causes economic and environmental concerns which necessitate a comprehensive search for biodegradable packaging materials. Brewer's spent grain (BSG) is an interesting by-product, which is one of the main wastes of beer production in Europe. BSG could offer added value in the food packaging sector owing to the significant amount generated annually, high biomaterials content, and low market value. Herein, the significance of various biorefinery techniques (physical, chemical, and biological) for the extraction of high-value products (such as protein, cellulose, hemicellulose, lignin, and phenolic compounds) from the BSG are comprehensively examined. BSG-derived biodegradable films and coatings for food packaging are critically evaluated. Finally, techno-economics, environmental impacts, energy consumption, regulations, challenges, and prospects are also critically evaluated. The best biorefinery system necessitates a balance between extraction efficiency, energy consumption, environmental impact, tangible upscaling, and operating cost. The mechanical dewatering of BSG before extraction, including the physical pretreatments, utilization of green solvents, the integration of the solvent recovery system, and the combination of two or more biorefinery techniques could reduce the energy requirements, greenhouse gas emissions, and increase the recovery yield of biomaterials. Cellulose, lignin, xylitol, and arabinoxylan are recommended as the most promising components from BSG for food packaging applications.