Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2006
Authors
Ingjerd Solfjeld Øystein JohnsenAbstract
We have examined shoot and root growth and the concentration of carbohydrates in seedlings of a northern (67 degrees N) and a southern (61 degrees N) ecotype of Betula pendula Roth. cultivated at root-zone temperatures of 2, 6, 12 and 17 degrees C. Three hydroponic experiments were conducted in controlled environments. We used three different pretreatments before seedlings were subjected to the experimental temperature treatments. Actively growing seedlings that were acclimated to the hydroponic solution for 3 weeks at a root temperature of 17 degrees C, continued to grow at all the experimental temperatures, with an expected increase in growth from 2 to 17 degrees C. However, if we started with ecodormant cold stored plants or used seedlings grown actively in perlite, no growth was observed at 2 degrees C and only minor growth was found at 6 degrees C. The highest root temperature always produced the best growth. The concentration of nonstructural carbohydrates was higher in seedlings grown at 2 degrees C than at 17 degrees C, and this is probably due to extensive incorporation of carbohydrates into cell walls and other structural elements at 17 degrees C. We found no evidence for differences between the two ecotypes in root growth, in timing of bud burst, but shoot growth terminated in the northern ecotype in the first experiment because the natural photoperiod was below the critical value. Our study highlights the importance of post-transplantation stress (planting check) related to root growth, and that root threshold temperatures may change according to the way plants are pretreated. Abstract: We have examined shoot and root growth and the concentration of carbohydrates in seedlings of a northern (67 degrees N) and a southern (61 degrees N) ecotype of Betula pendula Roth. cultivated at root-zone temperatures of 2, 6, 12 and 17 degrees C. Three hydroponic experiments were conducted in controlled environments. We used three different pretreatments before seedlings were subjected to the experimental temperature treatments. Actively growing seedlings that were acclimated to the hydroponic solution for 3 weeks at a root temperature of 17 degrees C, continued to grow at all the experimental temperatures, with an expected increase in growth from 2 to 17 degrees C. However, if we started with ecodormant cold stored plants or used seedlings grown actively in perlite, no growth was observed at 2 degrees C and only minor growth was found at 6 degrees C. The highest root temperature always produced the best growth. The concentration of nonstructural carbohydrates was higher in seedlings grown at 2 degrees C than at 17 degrees C, and this is probably due to extensive incorporation of carbohydrates into cell walls and other structural elements at 17 degrees C. We found no evidence for differences between the two ecotypes in root growth, in timing of bud burst, but shoot growth terminated in the northern ecotype in the first experiment because the natural photoperiod was below the critical value. Our study highlights the importance of post-transplantation stress (planting check) related to root growth, and that root threshold temperatures may change according to the way plants are pretreated.
Authors
Anne Straumfors Karl-Christian Nordby Wijnand Eduard Sonja KlemsdalAbstract
Inhalation of immunomodulating mycotoxins produced by Fusarium spp. that are commonly found in grain dust may imply health risks for grain farmers. Airborne Fusarium and mycotoxin exposure levels are mainly unknown due to difficulties in identifying Fusarium and mycotoxins in personal aerosol samples. We used a novel real-time PCR method to quantify the fungal trichodiene synthase gene (tri5) and DNA specific to F. langsethiae and F. avenaceum in airborne and settled grain dust, determined the personal inhalant exposure level to toxigenic Fusarium during various activities, and evaluated whether quantitative measurements of Fusarium-DNA could predict trichothecene levels in grain dust. Airborne Fusarium-DNA was detected in personal samples even from short tasks (10-60 min). The median Fusarium-DNA level was significantly higher in settled than in airborne grain dust (p < 0.001), and only the F. langsethiae-DNA levels correlated significantly in settled and airborne dust (r(s) = 0.20, p = 0.003). Both F. langsethiae-DNA and tri5-DNA were associated with HT-2 and T-2 toxins (r(s) = 0.24-0.71, p < 0.05 to p < 00.01) in settled dust, and could thus be suitable as indicators for HT-2 and T-2. The median personal inhalant exposure to specific toxigenic Fusarium spp. was less than 1 genome m(-3), but the exposure ranged from 0-10(5) genomes m(-3). This study is the first to apply real-time PCR on personal samples of inhalable grain dust for the quanti. cation of tri5 and species- specific Fusarium-DNA, which may have potential for risk assessments of inhaled trichothecenes.
Abstract
We describe the cloning and characterization of a single copy gene from Trichoderma atroviride P1 encoding a novel 30 kDa chitinase, Ech30. Ech30 is a family 18 chitinase showing low sequence similarity to other Trichoderma chitinases. Real-time quantitative RT-PCR studies revealed that expression of the ech30 gene was induced by the presence of Botrytis cinerea in plate confrontation assays, but hardly by chitin in liquid cultures. Studies of Ech30 purified from an Escherichia coli strain overexpressing the ech30 gene devoid of the leader sequence and a predicted intron, showed that the gene encodes an active chitinase, which, as expected for family 18 chitinases, is inhibited by allosamidin.
Abstract
The aim of this study was to compare two methods for non-destructive strength testing of wood by the use of dynamic modulus of elasticity (MOEdyn). The two methods are based on resonant vibration excitation and ultrasonic pulse excitation. Sound Pinus sylvestris L. sapwood samples treated with two copper-containing wood preservatives and two chitosan solutions were evaluated at two moisture levels. There was a significant correlation between the measurements given by the two MOEdyn test devices. An analysis of variance showed significant differences between the different treatments and between different moisture levels. Potential use of the non-destructive MOEdyn methods in durability testing is discussed.
Abstract
Temporal changes in the scores of selected soil fertility indices were studied over six years in three different cases of organic crop rotation located in southern, eastern and central Norway. The cropping history and the initial scores of fertility indices prior to conversion to organic cropping differed between the sites. Crop yields, regarded as an overall, integrating fertility indicator, were in all rotations highly variable with few consistent temporal trends following the first year after conversion. On the site in eastern Norway, where conversion followed several years of all-arable crop rotations, earthworm number and biomass and soil physical properties improved, whereas the system was apparently degrading with regard to P and K trade balances and contents in soil. On the other two sites, the picture was less clear. On the southern site, which had a relatively fertile soil before conversion, the contents of soil organic matter and K decreased during the six-year period, but the scores of other fertility indices showed no trends. On the site in central Norway, there were positive trends for earthworm-related indices such as worm biomass and tubular biopores, and negative trends for soil porosity. The results, especially those from the eastern site, illustrate the general difficulty in drawing conclusions about overall fertility or sustainability when partial indicators show divergent trends. Consequently, the study gave no unambiguous support to the initial working hypothesis that organic farming increases inherent overall soil fertility, but rather showed that the effect varied among indicators and depended on status of the cases at conversion. It is concluded that indicators are probably better used as tools to learn about and improve system components than as absolute measures of sustainability.
Authors
Tor Gunnar Vågen Masy-A A Andrianorofanomezana Salmata AndrianorofanomezanaAbstract
Soils of tropical forests are often inherently nutrient poor, although the extents of extremely infertile tropical forest soils are not as large as previous estimates may suggest. This paper presents findings from a study of change in soil quality in relation to deforestation and land use change in the highlands of Madagascar. A synthesis of some of the available research results related to soil characteristics of tropical forest, and their response to disturbance and conversion (i.e. deforestation) is made. The study was conducted in an area in the eastern highlands of Madagascar. The predominant soil types in the eastern highlands of Madagascar are Oxisols, which are acid and have a high content of low activity clays. The chemical characteristics of forest soils were found to be highly variable, with soil organic carbon (SOC) and total nitrogen (TN) contents ranging from 22.8 to 120.8 and 2.2 to 8.8 g kg(-1), respectively. Conversion of forest to cropland (tavy) reduced SOC contents by 23.8 g kg(-1) in the first year after clearing and by 11.3 g kg(-1) year(-1) on average in the first 3 years of cultivation. Mixed fallow systems recovered SOC at rates of about 6.5 g kg(-1) year(-1). Available phosphorus (P) and exchangeable base cations (Ca, Mg and K) increased after clearing as a result of biomass burning while cation exchange capacity is largely determined by SOC content and follows similar trends as SOC after clearing. The long term trend was, however, in the direction of significant decreases in available P while the sum of base cations showed little change relative to natural forest soils. (C) 2005 Elsevier B.V. All rights reserved.
Abstract
The effect of concentrate supplementation on nitrogen (N) intake and excretion in grazing lactating dairy cows was determined in three herds in Norway. Grazing trials were conducted with each herd in June and August for two consecutive years. The average supplementation was 1.8 (S.D. 2.1) kg DM/day, and the concentrate was based on grain with a N content ranging from 18.7 to 24 g/kg DM. Herbage DM and N intake were reduced with increasing supplementation, but total DM and N intake increased. Milk yield and protein content increased by 1.1 kg milk and 0.28 g protein per kg milk for each kg extra concentrate. Milk N excretion increased with increasing supplementation (6.5 g N/kg DM), and N utilisation improved by 11.7 g N per kg N intake per kg extra concentrate. Excretion of urine N and its share of total excreta N decreased by 4.0 and 9.2 g/kg concentrate, respectively. The reducing effect on urine N excretion of supplementation was, however, numerically low compared with other studies, most likely due to a high crude protein content of the concentrate used. (c) 2006 Elsevier B.V. All rights reserved.
Abstract
No abstract has been registered
Authors
T. Larssen E. Lydersen D. Tang Yi He Jixi Gao H. Liu Lei Duan H.M. Seip R.D. Vogt Jan Mulder M. Shao Yan-Hui Wang H. Shang X. Zhang Svein Solberg Wenche Aas Tonje Økland O. Eilertsen V. Angell Quanru Liu D. Zhao Renjun Xiang J. Xiao J. LuoAbstract
Acid rain emerged as an important environmental problem in China in the late 1970s. Many years of record economic growth have been accompanied by increased energy demand, greater coal combustion, and larger emissions of pollutants. As a result of significant emissions and subsequent deposition of sulfur, widespread acid rain is observed in southern and southwestern China. In fact, the deposition of sulfur is in some places higher than what was reported from the ?black triangle? in central Europe in the early 1980s. In addition, nitrogen is emitted from agriculture, power production, and a rapidly increasing number of cars. As a result, considerable deposition of pollutants occurs in forested areas previously thought to be pristine. Little is known about the effects of acid deposition on terrestrial and aquatic ecosystems in China. In this article, we present the current situation and what to expect in the future, largely on the basis of results from a five-year Chinese?Norwegian cooperative project. In the years ahead, new environmental challenges must be expected if proper countermeasures are not put into place.
Authors
Jørgen Aleksander Mølmann Olavi Junttila Øystein Johnsen Jorunn Elisabeth OlsenAbstract
Seedlings of trees with a free growth pattern cease growth when night-lengths become shorter than a critical value, and this critical night-length (CNL) decreases with increasing latitude of origin. In northern populations, the light quality also appears to play an important role and a clinal variation in requirement for far-red (FR) light has been documented. In this study we dissected the light quality requirements for maintaining growth in different latitudinal populations of Norway spruce (Picea abies (L.) H. Karst.) using light emitting diodes for red (R), FR and blue (B) light, as 12 h day extension to provide 24 h photoperiod. At equal spectral photon flux, FR light was more effective than R light in maintaining growth, and the requirement of both R and FR increased with northern latitude of origin. One-to-one mixtures of R and FR light were more effective in maintaining growth than either FR or R light alone, indicating a possible interaction between R and FR light maintaining growth. Using the blue light as day extension could not prevent growth cessation in any of the populations, but delayed the bud set slightly in all populations. Our results suggest that phytochrome(s) are the primary photoreceptors in high irradiance responses maintaining growth in Norway spruce seedlings.