Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2005
Authors
Carl Gunnar Fossdal Ari M. HietalaAbstract
The root-rot causing fungus Heterobasidion annosum can attack both spruce and pine trees and is the economically most damaging pathogen in northern European forestry. We have monitored the Heterobasidion annosum S-type (fairly recently named H. parviporum) colonization rate and expression of host chitinases and other host transcripts in Norway spruce material with differing resistances. Transcript levels of three chitinases, representing classes I, II and IV, were monitored with real-time PCR. We have also transferred a Class IV chitinase to Arabidopsis as well as its promotor in GFP and YFP reporter constructs. Ramets of two 33 -year-old clones differing in resistance were employed as host material and inoculation and wounding was performed. Multiplex real-time PCR detection of host and pathogen DNA was also performed to follow the colonization of the host tissues by the pathogen and the collapse in host DNA levels in infected regions. Host defense transcript levels, as an indicator of the host defense response, were monitored with singleplex real-time PCR. Three days after inoculation, comparable colonization levels were observed in both clones in the area immediately adjacent to inoculation. Fourteen days after infection, pathogen colonization was restricted to the area immediately adjacent to the site of inoculation for the strong clone (589), but had progressed further into the host tissue in the weak clone (409). Transcript levels of the class II and IV chitinases increased following wounding or inoculation, while the transcript level of the class Ichitinase declined following these treatments. Transcript levels of the class II and class IV chitinases were higher in areas immediately adjacent to the inoculation site in 589 than in similar sites in 409 three days after inoculation, suggesting that the clones differ in the rate of pathogen perception and host defense signal transduction. This an earlier experiments using mature spruce clones as substrate indicate that it is the speed of the host response and notmaximum amplitude of the host response that is the most crucial component in an efficient defense in Norway spruce toward pathogenic fungi such as H. annosum.
Abstract
Intensive monitoring plots of the ICP Forests gathered an amount of data about the ground vegetation in forest ecosystems throughout Europe. Each Country, applying different field techniques, conform to common rules of procedure, under the suggestions of a dedicated Expert Panel which implemented a Unified Coded Flora and comparability targets. Data series are foreseen to contribute to: definition of the forest ecosystem state and changes evaluation; assessment of the specific plant diversity at the ecosystems level. The contribution to scientific knowledge and to Global and Pan-European biodiversity initiatives and networks (ICP-IM, MCPFE, CBD, Forest BIOTA, ALTER-net, etc.) are also underlined. In spite of site-related data, first results (more than 670 plots, with large differences in plant diversity) depict the linkages with temperature, precipitation, dominant tree species and actual soil acidity. Nitrogen deposition seems to have some significant influence, which claims to further studies. Plant data series from ICP Forest’s plot, can be used for on-site confirmation of models including biodiversity k-factors and environment relations.
Authors
Per Otto Flæte Erlend Ystrøm Haartveit Kjell VadlaAbstract
No abstract has been registered
Abstract
No abstract has been registered
Abstract
The spruce bark beetle is the most serious pest of mature spruce trees in Eurasia. At low population densities it breeds in weakened or newly dead trees, but at high densities it becomes a tree killer. The exact factors triggering outbreaks are not fully understood, but climatic variables are important candidates. Populations in SE Norway have been monitored since 1979. Various time series methods are used to explore the role of climate in outbreaks of the spruce bark beetle in space and time, and to estimate the distribution of bivoltism under different scenarios of climate change. Large windfall events appear to be a major synchronizer of beetle outbreaks in areas subjected to regionalized weather systems, and the northern border of bivoltism may be moved towards north as a function of increasing temperature. Preliminary models of the population dynamics emphasize the frequency of large windfall events and phenological changes due to temperature increase. Final aims are to estimate the regional risks of forest damage under different scenarios of climate change, and to describe practices that may reduce the impact for forest managers.
Abstract
No abstract has been registered
Abstract
At least three mechanisms are known to cause synchrony among spatially separated insect populations: 1) dispersal among populations, 2) synchronous stochastic effects, often referred to as the Moran effect, and 3) trophic interactions with other species that are either themselves synchronized or mobile.The present study brings in the role of insect taxa for spatial synchrony. The spatial synchrony observed in several North American and Eurasian epidemic bark beetles was compared with patterns of synchrony in outbreaks of defoliating forest Lepidoptera, revealing a marked difference between these two major insect taxa.The bark beetles exhibited a generally lower degree of spatial synchrony than the Lepidoptera, possibly because bark beetles are synchronized by different weather variables that are acting on a smaller scale than those affecting the Lepidoptera, or because inherent differences in their dynamics leads to more cyclic oscillations and hence more synchronous spatial dynamics in the Lepidoptera. Among the epidemic bark beetles tested, spatial synchrony of outbreaks in the Eurasian spruce bark beetle Ips typographus was significantly higher than for the other species.
Abstract
Wood samples treated with ammonium copper quat (ACQ 1900 and ACQ 2200), chromated copper arsenate (CCA), Tanalith E 3491 and Wolmanit CX-8 have been studied in accelerated weathering experiments. The weathering experiment was performed by cycles of 2 h UV-light irradiation followed by water spray for 18 min. The changes on the surface of the weathered samples were characterized by roughness and color measurements on the samples with 0, 200, 400 and 600 h of total weathering.The objective of this study was to investigate the changes created by weathering on impregnated wood with several different wood preservatives. This study was performed on the accelerated weathering test cycle, using UVirradiation and water spray in order to simulate natural weathering. Surface roughness and color measurement was used to investigate the changes after several intervals (0200400600 h) in artificial weathering of treated and untreated wood.
Authors
Harald Kvaalen Ola Gram Dæhlen Anne Tove Rognstad Borgny Sveen Grønstad Ulrika EgertsdotterAbstract
Seeds of Abies lasiocarpa (Hook.) Nutt. (subalpine fir) were dissected, and the different parts were analyzed for elemental composition. The data were used to design a novel growth medium for initiation of somatic embryogenesis. Embryogenic cultures were initiated from immature zygotic embryos from six open-pollinated families of A. lasiocarpa on three different media.The frequency of initiation was the highest in early to mid-July when the zygotic embryo explants were ca. 0.8 mm long. Thereafter the response declined rapidly.The culture media did not significantly affect the initiation frequencies, but the subsequent growth and culture survival was dependent on the culture medium. On the Schenk Hildebrandt medium, many cultures ceased to grow and died. Several of the decaying cultures were rescued after transfer to one of the new media. Proliferating cultures could be stimulated to produce mature embryos. Of 2510 mature somatic embryos, 212 (8.4%) converted to plants, and 35 plants have grown over two periods.
Authors
Kåre Olav VennAbstract
No abstract has been registered