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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2009

Abstract

The Scots pine is one of the most important raw materials for the wood industry. The pine is straight and strong, and easily preservation treated. The objective of this study was to describe and analyse the accuracy of diameter observations by varying technologies, and to have a first indication for the applicability of RFID tracing logs in the pole industry. Observations were made on barked poles with calliper, 2D scanner and 3D scanner. The local variation in diameter can be indicated by a standard deviation of 3 to 5 mm for repeated observation in almost the same location, and the same magnitude for actual diameter profile deviation from a smoothed profile. In a 3D scanner, the amount of observed data is huge, making it a challenge to analyse, verify and calibrate the equipment. Nevertheless, the accuracy seems acceptable for observations on barked logs. The RFID technology might find uses in permanent applications, but it is still rather work consuming and expensive, and the commercial profit has so far not been identified.

Abstract

To identify differentially expressed genes of the white-rot fungus Heterobasidion parviporum subtractive cDNA libraries were constructed using suppressive subtraction hybridization (SSH) technique with RNA extracted from an advanced stage of decay area and from colonization front next to the reaction zone of the stem of a mature Norway spruce trees. Besides cytochrome P450s and proteins with unknown function, the SSH libraries constructed contained genes involved in basic cellular processes, andcell wall degradation. To examine the role of selected candidate genes three trees, showing a variable degree of wood decay, were used for real-time RT-PCR profiling of candidate genes. In the decay transition areas the study revealed activity centers that showed remarkable similarity in the transcript profiles of monitored genes.