Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2011
Authors
Clive Kaiser Philip B. Hamm Stacy Gieck Nicholas David Lynn Long Mekjell Meland J. Mark ChristensenAbstract
In vitro dose responses of several calcium and potassium salts were determined on some commercially significant plant pathogens including: Helminthosporium solani, Fusarium oxysporum f. sp. pisi race 2, Colletotricum coccodes, Phytophthora cactorum, Phytophthora cinnamomi, Phytophthora erythroseptica, Phytophthora infestans, Phytophthora megasperma, Pythium ultimum and Venturia inaequalis. Mycelial growth inhibition was both salt-specific and dose-related. Pythium ultimum was completely inhibited by ≥75 mg·L-1 calcium propionate, but needed ≥ 300 mg·L-1 of calcium acetate and ≥ 40 mL·L -1 of potassium silicate for complete inhibition. Phytophthora infestans was completely inhibited by ≥150 mg·L-1 calcium acetate, ≥150 mg·L-1 calcium propionate or ≥ 5 mL·L-1 potassium silicate. Phytophthora cactorum was completely inhibited by ≥ 300 mg·L-1 calcium propionate, but required ≥ 600 mg·L-1 calcium acetate and ≥10 mL·L-1 potassium silicate for complete inhibition. Phytophthora cinnamomi was completely inhibited by calcium propionate at ≥ 600 mg·L-1, or by ≥10 mL·L-1 potassium silicate. Only potassium silicate inhibited Phytophthora megasperma, Phytophthora erthroseptica, V. inequalis and H. solani at concentrations of ≥ 5mL·L-1, ≥ 20 mL·L-1, ≥ 40 mL·L-1 or ≥ 80mL·L-1 respectively. Potassium acetate did not completely inhibit any of the pathogens in this study when tested at concentrations ≤ 1200 mg·L-1.
Authors
Anne-Kristin Løes Benjamin* Nölting*Abstract
No abstract has been registered
Authors
Tao Zhao Paal Krokene Jiang Hu Erik Christiansen Niklas Björklund Bo Långström Halvor Solheim Anna-Karin Borg-KarlsonAbstract
Background: Tree-killing bark beetles (Coleoptera, Scolytinae) are among the most economically and ecologically important forest pests in the northern hemisphere. Induction of terpenoid-based oleoresin has long been considered important in conifer defense against bark beetles, but it has been difficult to demonstrate a direct correlation between terpene levels and resistance to bark beetle colonization. Methods: To test for inhibitory effects of induced terpenes on colonization by the spruce bark beetle Ips typographus (L.) we inoculated 20 mature Norway spruce Picea abies (L.) Karsten trees with a virulent fungus associated with the beetle, Ceratocystis polonica (Siem.) C. Moreau, and investigated induced terpene levels and beetle colonization in the bark. Results: Fungal inoculation induced very strong and highly variable terpene accumulation 35 days after inoculation. Trees with high induced terpene levels (n = 7) had only 4.9% as many beetle attacks (5.1 vs. 103.5 attacks m22) and 2.6% as much gallery length (0.029 m m22 vs. 1.11 m m22) as trees with low terpene levels (n = 6). There was a highly significant rank correlation between terpene levels at day 35 and beetle colonization in individual trees. The relationship between induced terpene levels and beetle colonization was not linear but thresholded: above a low threshold concentration of ,100 mg terpene g21 dry phloem trees suffered only moderate beetle colonization, and above a high threshold of ,200 mg terpene g21 dry phloem trees were virtually unattacked. Conclusion/Significance: This is the first study demonstrating a dose-dependent relationship between induced terpenes and tree resistance to bark beetle colonization under field conditions, indicating that terpene induction may be instrumental in tree resistance. This knowledge could be useful for developing management strategies that decrease the impact of tree-killing bark beetles.
Authors
Axel Schmidt Raimund Nagel Trygve Krekling Erik Christiansen Jonathan Gershenzon Paal KrokeneAbstract
Norway spruce (Picea abies) defends itself against herbivores and pathogens by formation of traumatic resin ducts filled with terpenoid-based oleoresin. An important group of enzymes in terpenoid biosynthesis are the short-chain isoprenyl diphosphate synthases which produce geranyl diphosphate (C10), farnesyl diphosphate (C15), and geranylgeranyl diphosphate (C20) as precursors of monoterpenes, sesquiterpenes, and diterpene resin acids, respectively. After treatment with methyl jasmonate (MJ) we investigated the expression of all isoprenyl diphosphate synthase genes characterized to date from Norway spruce and correlated this with formation of traumatic resin ducts and terpene accumulation. Formation of traumatic resin ducts correlated with higher amounts of monoterpenes, sesquiterpenes and diterpene resin acids and an upregulation of isoprenyl diphosphate synthase genes producing geranyl diphosphate or geranylgeranyl diphosphate. Among defense hormones, jasmonate and jasmonate-isoleucine conjugate accumulated to higher levels in trees with extensive traumatic resin duct formation, whereas salicylate did not. Jasmonate and ethylene are likely to both be involved in formation of traumatic resin ducts based on elevated transcripts of genes encoding lipoxygenase and 1-aminocyclopropane-1-carboxylic acid oxidase associated with resin duct formation. Other genes involved in defense signalling in other systems, mitogen-activated protein kinase3 and nonexpressor of pathogenesis-related gene1, were also associated with traumatic resin duct formation. These responses were detected not only at the site of MJ treatment, but also systemically up to 60 cm above the site of treatment on the trunk.
Authors
Arnstein Øvrum Kyrre RickertsenAbstract
No abstract has been registered
Abstract
No abstract has been registered
Abstract
Due to sewage sludge application on soils, terrestrial ecosystems are very likely to be exposed to silver nanoparticles (AgNPs) and it is thus important to understand the behavior of Ag NPs once in contact with soil components. The aim of this work was to compare the behavior of silver under three forms, silver nitrate, citrate stabilized AgNPs (C-ANPs) and uncoated AgNPs (P-AgNPs), in two soils with contrasting organic matter content, and over time. The physical and chemical properties of the studied soils as well as the nanoparticles size, shape, crystallographic structure and specific surface area were characterized. Soil samples were spiked with silver nitrate, C-AgNPs or P-AgNPs, and let for ageing 2 hours, 2 days, 5 weeks or 10 weeks before they were submitted to sequential extraction. The ionic silver solution and the two AgNPs types were radiolabeled so that we could detect and quantify silver by gamma spectrometry by measuring the 110mAg tracer in the different sequential extraction fractions. We thereby obtained for each silver form, soil type and time point a distribution of silver in the different fractions. Silver was generally more mobile in the mineral soil, although the fractionation patterns were very different for the three silver types in both cases. Over 20% of the total C-AgNPs concentration were water soluble in both soils (<5% for AgNO3 and P-AgNPs) the first two days after spiking, but the fraction decreased to trace levels thereafter. This was compensated by an increase in the reducible fraction. Regarding P-AgNPs, 80% were not extractable at all, but contrary to AgNO3 and C-AgNPs, the water soluble and ion exchangeable fractions did not decrease over time in the mineral soil, and even increased in the organic soil.
Authors
Atle HaugeAbstract
No abstract has been registered
Authors
Tor J. JohansenAbstract
No abstract has been registered
Authors
Cecilie Marie Mejdell Grete Helen Meisfjord Jørgensen Therese Rehn Linda J Keeling Kjersti Elisabeth Fremstad Knut Egil BøeAbstract
No abstract has been registered