Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2020
Authors
Anita SønstebyAbstract
No abstract has been registered
Abstract
Growth cessation and floral initiation in black currant and red raspberry are jointly controlled by the interaction of temperature and short-day (SD) conditions, and the processes coincide in time in both natural and controlled environments. The critical photoperiods for the two successional responses were found to be approximately 15 and 16 h, respectively, for a range of Western-European black currant cultivars. Both cessation of growth and floral initiation are promoted and enhanced by increasing temperature in the 9 to 24°C range. In contrast, biennial-fruiting red raspberry has a maximum temperature limit for growth cessation and floral initiation. At temperatures above 16°C, most cultivars grow and remain vegetative regardless of day length conditions, at 12 to 16°C they cease growing and initiate flower primordia in photoperiods <15 h, while at temperatures ≤12°C they cease growing and initiate floral primordia regardless of day length. In the annual-fruiting (primocane) types of red raspberry on the other hand, floral initiation is not constrained by high temperature, but readily takes place at temperatures up to 30°C. In addition, floral initiation is also enhanced by long day (LD) conditions in most of these cultivars. Another fundamental physiological difference is that while floral primordia of the biennial types become dormant after initiation, they proceed directly to anthesis in the annual-fruiting types. Chilling at -5°C, and in the -5 to +5°C temperature range were found to be optimal for breaking of bud dormancy and promotion of flowering in black currant and red raspberry, respectively. In black currant, 14 weeks of chilling were optimal, while for raspberry, 20 or more weeks were required for full dormancy release and promotion of flowering along the entire length of the raspberry cane. The consequences of climate warming for the production of these species in different climatic regions are discussed.
Authors
Paul Eric AspholmAbstract
No abstract has been registered
Authors
Hans Martin Hanslin Trygve S. Aamlid Ellen Johanne Svalheim Johannes Kollmann Geir Kjølberg Knudsen Harald BratliAbstract
No abstract has been registered
Authors
Hans Martin Hanslin Trygve S. Aamlid J. Kollmann T. Lennartson Ellen Johanne Svalheim Eveliina KallioniemiAbstract
No abstract has been registered
Authors
Hans Martin Hanslin Trygve S. Aamlid Ellen Johanne Svalheim Geir Kjølberg Knudsen Johannes Kollmann Harald Bratli Jörgen WissmannAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Ranjana Pathak Åshild Ergon Arne Stensvand Hans Ragnar Gislerød Knut Asbjørn Solhaug Lance Cadle-Davidson Aruppillai SuthaparanAbstract
Powdery mildews can be controlled by brief exposure to ultraviolet (UV) radiation with devastating effect on their developmental stages including conidia germination. The treatment effect can be impaired by subsequent exposure to UV-A/blue light. UV-A/blue light-activated photolyase may be responsible for this and therefore we tested the function of three cryptochrome/photolyase family (CPF)-like genes (OINE01015670_T110144, OINE01000912_T103440, and OINE01005061_T102555) identified in the obligate biotrophic fungus Pseudoidium neolycopersici, the cause of tomato powdery mildew. A photolyase-deficient mutant of Escherichia coli transformed with coding sequence of OINE01000912_T103440 and exposed to brief (UV)-C treatment (peak emission at 254 nm) showed photoreactivation and cell survival when exposed to subsequent blue light, indicating complementation of photolyase activity. In contrast, the same photolyase-deficient E. coli transformed with the coding sequences of other two CPF-like genes did not survive this treatment, even though their expression were confirmed at protein level. This confirmed that OINE01000912_T103440 is a gene encoding photolyase, here named PnPHR1, with functionality similar to the native photolyase in E. coli, and classified as a class I cyclobutane pyrimidine dimer (CPD) photolyase. Modeling of the 634-amino acid sequence of PnPHR1 suggested that it is capable of binding flavin adenine dinucleotide (FAD) and methenyltetrahydrofolate (MTHF). However, spectroscopic data of the protein produced in an E. coli expression system could only reveal the presence of a reduced form of FAD, i.e., FADH– as an intrinsic chromophore. Within the tested wavelength range of 365–525 nm, the survival of photolyase-deficient mutant E. coli transformed with PnPHR1 showed a broad action spectrum from 365 to 454 nm. This was very similar to the previously characterized action spectrum for survival of P. neolycopersici conidia that had been treated with UV-C. Quantitative RT-PCR revealed that the expression of PnPHR1 in P. neolycopersici conidia was induced by UV-C, and peak expression occurred 4 h after brief UV-C treatment. The expression of PnPHR1 was repressed when incubated in red light after the UV-C treatment, but not when incubated in UV-A/blue light. The results may explain why the disease-reducing effect of short wavelength UV is impaired by exposure to UV-A and blue light.
Authors
Leonardo H. Teixeira Florencia A. Yannelli Gislene Ganade Johannes KollmannAbstract
Ecosystem properties can be positively affected by plant functional diversity and compromised by invasive alien plants. We performed a community assembly study in mesocosms manipulating different functional diversity levels for native grassland plants (communities composed by 1, 2 or 3 functional groups) to test if functional dispersion could constrain the impacts of an invasive alien plant (Solidago gigantea) on soil fertility and plant community biomass via complementarity. Response variables were soil nutrients, soil water nutrients and aboveground biomass. We applied linear mixed-effects models to assess the effects of functional diversity and S. gigantea on plant biomass, soil and soil water nutrients. A structural equation model was used to evaluate if functional diversity and invasive plants affect soil fertility directly or indirectly via plant biomass and soil pH. Invaded communities had greater total biomass but less native plant biomass than uninvaded ones. While functional diversity increased nutrient availability in the soil solution of uninvaded communities, invasive plants reduced nutrient concentration in invaded soils. Functional diversity indirectly affected soil water but not soil nutrients via plant biomass, whereas the invader reduced native plant biomass and disrupted the effects of diversity on nutrients. Moreover, invasive plants reduced soil pH and compromised phosphate uptake by plants, which can contribute to higher phosphate availability and its possible accumulation in invaded soils. We found little evidence for functional diversity to constrain invasion impacts on nutrients and plant biomass. Restoration of such systems should consider other plant community features than plant trait diversity to reduce establishment of invasive plants.
Authors
Radosav Cerovic Milica Fotiric Akšic Milena Dordevic Mekjell MelandAbstract
Since the European pear (Pyrus communis L.) is a self-incompatible fruit species, synchrony and compatibility between female parts of the mother plant and male gametes from the pollen donor must be fulfilled. Besides pollination and fertilization, normal embryo and zygote development is one of the prerequisites for the satisfactory yields in pears. The main goal of this experiment was to investigate the functionality of embryo sacs and the embryo’s early stages of growth in relation to the fruit set of diploid (‘Celina’) and the triploid (‘Ingeborg’) pear cultivars under specific Norwegian climatic conditions. For this purpose, flowers were collected at the beginning of flowering, and on the third, sixth, ninth, and twelfth days after the beginning of this phenophase for two consecutive years. Ovaries were dehydrated, embedded in paraffin wax, sectioned, stained, and observed under the light microscope. In the analyzed cultivars, results showed different tendencies in embryo sac development and degradation processes, in both experimental years, which is probably due to the genetic background of the examined cultivars. Also, fertilization success and fruit set were higher in the second year of study due to the higher average temperature during the flowering period. Diploid cultivar ‘Celina’ showed much better adaptation to high temperatures in relation to triploid cultivar ‘Ingeborg’.