Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2013
Abstract
No abstract has been registered
Abstract
Three methods for extracting DNA were tested on otoliths, scales, fins, and gill tissue from European whitefish [Coregonus lavaratus (L.)]. The aim was to find time-efficient and affordable ways to simultaneously extract DNA suitable for conservation genetic studies from a large number of samples and different tissues. A rapid low-cost method led to 97 % success of microsatellite amplification in otoliths and 100 % in scales. High amplification success was achieved with fin (97 %) and gill (99 %) tissue using a salt lysis-based protocol. A commercial extraction kit delivered good results with all tissues. The findings are useful for conservation genetic studies using both contemporary and archived samples.
Authors
Ivano Brunner Isabella Børja Lise Dalsgaard Gaby Deckmyn Alf Ekblad Douglas L. Godbold Irene Guerra Avalos Martin Lukac Karsten Kalbitz Kaie Kriiska Ivika Ostonen Andrea Schnepf Primož Simončič Elena VanguelovaAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Isabella Børja Douglas Lawrence Godbold Michael Bambrick Lise Dalsgaard Jan Svetlik Nina Elisabeth NagyAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Trond Rafoss Jarle Skahjem John Atle Johansen Ståle Johannessen Sekhar Udaya Nagothu Inger Sundheim Fløistad Arild SlettenAbstract
No abstract has been registered
Abstract
Local climate conditions have a major influence on the biological decomposition of wood. To examine the influence of different temperature regimes on wood decay caused by the brown rot fungus Postia placenta in wood with differing natural durability, sapwood (sW) and heartwood (hW) of Scots pine, inoculated mini-blocks were incubated for up to 10 weeks at temperatures conducive or above optimal to wood decay. We profiled mass loss (ML) and wood composition, and accompanying changes in wood colonization and transcript level regulation of fungal candidate genes. The suppressive effect of suboptimal temperature on wood decay caused by P. placenta appeared more pronounced in Scots pine hW with increased durability than in sW with low decay resistance. The differences between sW and hW were particularly pronounced for cultures incubated at 30°C: unlike sW, hW showed no ML, poor substrate colonization and marker gene transcript level profiles indicating a starvation situation. As brown rot fungi show considerable species-specific variation in temperature optima and ability to mineralize components that contribute to wood durability, interactions between these factors will continue to shape the fungal communities associated to wood in service.
2012
Abstract
No abstract has been registered
Abstract
No abstract has been registered