Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2012
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No abstract has been registered
Authors
Arne Sæbø Robert Popek Barbara Nawrot Hans Martin Hanslin Helena Gawronska Stanislaw W GawronskiAbstract
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No abstract has been registered
Authors
Muath Alsheikh-Yousef Gage Koehler Robert Wilson Jens Rohloff Anita Sønsteby Jahn Davik Stephen K, RandallAbstract
A crucial consideration for strawberry producers in Norway and other northern countries is winter freezing damage. A long-term goal of the Norwegian strawberry breeding is to increase winter hardiness and to improve fruit quality. Due to the complexity involved in regulating and enhancing freezing tolerance, the progress in the improvement of cultivars using traditional screening methods have had limited success. Thus, the development of molecular markers for freezing hardiness would facilitate the selection work for this trait. In this effort, we have developed and adopted state-of-art molecular tools to investigate cold response in strawberry plants during the acclimation phase resulting in the identification of a large number of genes, proteins, and distinct metabolites that correspond to cold/freezing tolerance in strawberry. To identify proteins responsible for freezing tolerance in strawberry we have examined alterations in protein levels in strawberry varieties that differ in cold tolerance using either 2-DE gel analysis followed by LC-MS/MS analysis or a shotgun MS/MS approach. Proteomic analysis suggested 30 potential biomarkers that showed significant changes in the cultivated strawberry in response to cold. In addition, GC-MS-based metabolite profiling revealed the up-regulation of carbohydrates, polyols, amino acids, TCA intermediates, and other distinct secondary metabolites after cold treatment. Transcriptional analysis of the cold acclimated samples also confirmed the regulation upon cold-treatment with varietal differences in strawberry. Moreover, several F2-populations from the model F. vesca parents diverging in cold tolerance have been developed in order to facilitate mapping of QTLs by performing GBS analyses. The knowledge attained from these endeavors is expected to expedite breeding of strawberries to achieve freezing tolerant lines and provide an integrative understanding of the molecular pathways that underlie this characteristic. * Rohloff et al. (2012) Metabolite profiling reveals novel multi-level cold responses in the model Fragaria vesca. Phytochemistry 79:99-109. * Koehler et al. (2012) Proteomic study of low temperature responses in strawberry cultivars (Fragaria x ananassa) that differ in cold tolerance. Plant Physiology 159:1787–1805 * Davik et al., (2012) Low temperature tolerance in diploid strawberry species (Fragaria ssp.) and its correlation to alcohol dehydrogenase levels, dehydrin levels, and central metabolism constituents. Planta (in press; DOI: 10.1007/s00425-012-1771-2).
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No abstract has been registered
Abstract
Norwegian goat milk production is based on summer grazing on diverse forest or alpine rangeland, and the quality of these pastures is important for milk quantity and quality. We used n-alkanes and long chained alcohols found in plant wax as markers to estimate diet composition in goats grazing on a heterogeneous rangeland during two periods in summer; early (beginning of July) and late (end of August). The goats were fitted with GPS collars that recorded their position. Preliminary results show a diverse diet, where ferns, sedges, blueberry (Vaccinium myrtillis) and birch were preferred in early summer. In late summer the diet was particularly diverse, coinciding with a general decline in plant quality.
Abstract
No abstract has been registered
Abstract
Norwegian goat milk production is based on summer grazing on diverse forest or alpine rangeland, and the quality of these pastures is important for milk quantity and quality. We used n-alkanes and long chained alcohols found in plant waxes as markers to estimate diet composition in goats grazing on a heterogeneous rangeland during two periods in summer; early (beginning of July) and late (end of August). Some of the goats were fitted with GPS collars that recorded their position. Preliminary results show a diverse diet, where ferns, sedges, blueberry (Vaccinium myrtillis) and birch (Betula pubescens) were preferred in early summer. In late summer the diet was particularly diverse, coinciding with a general decline in plant quality.
Authors
Randi Seljåsen Per Lea Torfinn Torp Hugh Riley Erling Berentsen Mette Thomsen Gunnar BengtssonAbstract
No abstract has been registered
Authors
Jahn Davik Gage Koehler Britta From Torfinn Torp Jens Rohloff Petter Eidem Robert Wilson Anita Sønsteby Stephen K, Randall Muath Alsheikh-YousefAbstract
The use of artificial freezing tests, identification of biomarkers linked to or directly involved in the low-temperature tolerance processes, could prove useful in applied strawberry breeding. This study was conducted to identify genotypes of diploid strawberry that differ in their tolerance to low-temperature stress and to investigate whether a set of candidate proteins and metabolites correlate with the level of tolerance. 17 Fragaria vesca, 2 F. nilgerrensis, 2 F. nubicola, and 1 F. pentaphylla genotypes were evaluated for low-temperature tolerance. Estimates of temperatures where 50 % of the plants survived (LT50) ranged from −4.7 to −12.0 °C between the genotypes. Among the F. vesca genotypes, the LT50 varied from −7.7 °C to −12.0 °C. Among the most tolerant were three F. vesca ssp. bracteata genotypes (FDP821, NCGR424, and NCGR502), while a F. vesca ssp. californica genotype (FDP817) was the least tolerant (LT50 −7.7 °C). Alcohol dehydrogenase (ADH), total dehydrin expression, and content of central metabolism constituents were assayed in select plants acclimated at 2 °C. The LT50 estimates and the expression of ADH and total dehydrins were highly correlated (r adh = −0.87, r dehyd = −0.82). Compounds related to the citric acid cycle were quantified in the leaves during acclimation. While several sugars and acids were significantly correlated to the LT50 estimates early in the acclimation period, only galactinol proved to be a good LT50 predictor after 28 days of acclimation (r galact = 0.79). It is concluded that ADH, dehydrins, and galactinol show great potential to serve as biomarkers for cold tolerance in diploid strawberry.