Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2017
Authors
Victor Phani Tagginahalli N. Shivakumara Keith Davies Uma RaoAbstract
Root-knot nematode (RKN) Meloidogyne incognita is an economically important pest of crops. Pasteuria penetrans, is a nematode hyperparasitic bacterium capable of suppressing the reproduction of RKN and thereby useful for its management. Secreted fatty acid and retinol-binding proteins are unique in nematodes and are engaged in nutrient acquisition, development and reproduction; they are also a component of the nematode cuticle and thought to be involved in the interface between hosts and parasites. Attachment of endospores to the cuticle of second stage juveniles of RKN is the primary step of infection and several factors have been identified to facilitate attachment. In this study, the full length of Mi-far-1 (573 bp) was cloned from M. incognita and characterized. Analysis revealed that the Mi-far-1 was rich in α-helix structure, contained a predicted consensus casein kinase II phosphorylation site and a glycosylation site. Quantitative PCR showed the highest expression in the fourth stage juveniles and in situ hybridization revealed the presence of Mi-far-1 mRNA in the hypodermis below the cuticle. Single copy insertion pattern of Mi-far-1 in M. incognita genome was detected by Southern blotting. Knockdown of Mi-far1 showed significantly increased attachment of P. penetrans’ endospores on juvenile cuticle surface and also affected host finding, root infection and nematode fecundity.
Authors
John Christian Gaby Espen Govasmark Live Heldal Hagen Lisa Paruch Linn Solli Phillip Pope Svein Jarle HornAbstract
No abstract has been registered
Academic – Microalgal proteins for feed, food and health
M. Hayes, Hanne Skomedal, Kari Skjånes, ...
Authors
M. Hayes Hanne Skomedal Kari Skjånes H. Mazur-Marzec A. Torunska-Sitarz M. Catala M. Isleten Hosoglu M. García-VaqueroAbstract
No abstract has been registered
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No abstract has been registered
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In Scandinavia, pasture for dairy herds with automatic milking (AM) is frequently offered purely for exercise and recreation, rather than as a feed-source. In the present study, cows in an AM-system with 12 h nightly outdoor-access from summer solstice until mid-September were offered either fresh production pasture (treatment P; ≥15 kg dry matter (DM) cow‑1 nightly, combined with 6 kg DM grass silage daytime) or exercise pasture (treatment E; <1 kg DM cow‑1 combined with ad libitum silage allowance day and night). Treatment showed a significant effect on milk yield (P:31.3, E:33.0 kg, P=0.05), and a tendency for milking frequency (P:2.25, E: 2.37 milkings × day‑1, P=0.06). Group P spent more time outdoors than E, 4.0 and 3.2 h, respectively (P<0.001). Cows in P grazed approximately 2.5 h throughout the season, while E grazed less overall, 0.6 h (P<0.001) and decreased their time spent grazing over the season (1.0 to 0.3 h). In conclusion, night-time pasture is poorly exploited by cows, irrespective of the quantity of both of pasture and silage that are available.
Authors
K. Marijanusic M. Manojlovic D. Bogdanovic R. Cabilovski Peder LombnæsAbstract
In order to evaluate the mineral composition of forage crops in respect to dairy cow nutrition 40 soil and corresponding plant (alfalfa, grasses and silage corn) samples were collected from 15 locations in Serbia and analyzed for the concentration of macro- (P, K, and Ca) and microelements (Mn, Cu, Zn, Fe, Co, Se, and Mo). On average, the soils were well provided with the studied elements from the aspect of plant nutrition, but the analyzed fodder crops could not secure suffi cient amounts of Cu, Zn, Se, and Ca for dairy cow nutrition. Principal components analysis was applied in order to determine the connection between the concentrations of macro- and microelements in forage crops and their grouping into components responsible for most of the variability in mineral content. The mineral composition of alfalfa was defi ned by three components (Se, Zn, and Cu) which accounted for the largest part of the established variability. The variability of mineral composition of grasses was defi ned by four components (Zn, K, Se, and P) and that of silage corn by the concentrations of Fe, Mn, and K.
Authors
Inger HansenAbstract
No abstract has been registered
Authors
Monica Suarez Korsnes Reinert KorsnesAbstract
The marine toxin yessotoxin (YTX) can cause various cytotoxic effects depending on cell type and cell line. It is well known to trigger distinct mechanisms for programmed cell death which may overlap or cross-talk. The present contribution provides the first evidence that YTX can cause genotoxicity and induce mitotic catastrophe which can lead to different types of cell death. This work also demonstrates potential information gain from non-intrusive computer-based tracking of many individual cells during long time. Treatment of BC3H1 cells at their exponential growth phase causes atypical nuclear alterations and formation of giant cells with multiple nuclei. These are the most prominent morphological features of mitotic catastrophe. Giant cells undergo slow cell death in a necrosis-like manner. However, apoptotic-like cell death is also observed in these cells. Electron microscopy of treated BC3H1 cells reveal uncondensed chromatin and cells with double nuclei. Activation of p-p53, p-H2AX, p-Chk1, p-ATM, and p-ATR and down-regulation of p-Chk2 indicate DNA damage response and cell cycle deregulation. Micronuclei formation further support this evidence. Data from tracking single cells reveal that YTX treatment suppresses a second round of cell division in BC3H1 cells. These findings suggest that YTX can induce genomic alterations or imperfections in chromosomal segregation leading to permanent mitotic failure. This understanding extends the list of effects from YTX and which are of interest to control cancer and tumor progression.