Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2004
Authors
Peder Gjerdrum G. Arrighetti Mauro BernabeiAbstract
Resin pocket is a natural property considered one of the main drawbacks of spruce timber, particularly important for appearance and joinery applications. The resins, viscous by nature, might only be solidified at temperatures superior to conventional kiln drying.Induced in the active cambium by traumas of unknown origin, size as well as location of resin pockets are random. Surrounding annual rings appear unaffected, while on the microscopic scale discrepancies in cell structure are easily recognised. Ca. 200 resin pockets per m3 timber have been reported in Scandinavian timber. More resin pockets were experienced in boards sawn from the ca. 16% of logs demonstrating resin pocket in the crosscuts.
Authors
Svein SolbergAbstract
Summer drought, i.e. unusually dry and warm weather, has been a significant stress factor for Norway spruce in southeast Norway during the 14 years of forest monitoring. Dry and warm summers were followed by increases in defoliation, discolouration of foliage, cone formation and mortality. The causal mechanisms are discussed. Most likely, the defoliation resulted from increased needle-fall in the autumn after dry summers.During the monitoring period 19882001, southeast Norway was repeatedly affected by summer drought, in particular, in the early 1990s. The dataset comprised 455 Forest officers plots with annual data on crown condition and mortality. Linear mixed models were used for estimation and hypothesis testing, including a variancecovariance structure for the handling of random effects and temporal autocorrelation.
Abstract
No abstract has been registered
Abstract
The quantitative expression and the regulation of chitinase-encoding genes ech30, ech42 and nag1 in Trichoderma atroviride P1 under varying growth conditions were investigated using real-time RTPCR, principle component and multivariate analyses. Twelve media combinations including 0.1% and 3% glucose as carbon source and no (0 mmol/L), low (10 mmol/L) and high (100 mmol/L)ammonium acetate as nitrogen source combined with or without colloidal chitin at 3 time intervals and 2 replications were applied to current study. The real-time RT-PCR analysis showed that the expression of ech30, ech42 and nag1 was regulated by the interaction of nitrogen, glucose and chitin under different growth conditions. The highest and earliest expressions of ech30 were induced by glucose and nitrogen starvation i.e. 0.1% glucose and 10 mmol/L ammonium acetate in the growth media. This was also the case for ech42 and nag1 but at a relatively low level. In contrast, high (3 %) glucose and high (100 mmol/L) ammonium acetate concentrations repressed the expression of all the genes studied. These results were confirmed by principle component and multivariate analyses.The effect of chitin on ech30, ech42 and nag1 expression varied depending on the concentrations of glucose and ammonium acetate.
Authors
Erik Jautris Joner D. Hirmann O.J.H. Szolar D. Todorovic C. Leyval A.P. LoibnerAbstract
An experiment was conducted to distinguish priming effects from the effects of phytoremediation of a creosote-polluted soil. The concentration of 13 polycyclic aromatic hydrocarbons (PAHs), and their combined soil toxicity (using four bioassays), was determined on recently excavated, homogenized soil and on such soil subjected to a time-course phytoremediation experiment with lucerne.The results showed a high priming effect, with minor positive and synergistic effects of planting and fertilization on PAH degradation rates. At the end of the experiment, PAH degradation reached 86% of the initial 519 mg PAHs kg-1. Two of the four toxicity tests (bioluminescence inhibition and ostracod growth inhibition) corroborated the chemical data for residual PAHs, and indicated a significant reduction in soil toxicity.We conclude that priming effects can easily surpass treatment effects, and that an unintentional pre-incubation that ignores these effects can jeopardize the full quantitative assessment of in situ bioremediation of contaminated soil.
Abstract
Chitosan, a derivate of the natural amino polysaccharide chitin, has proven effective as a potential environmentally benign antimicrobial component. Few studies have focused on chitosan applied to wood against wood inhabiting and decaying fungi.In these screening studies several mycological experiments were performed to screen chitosan as a potential wood protecting agent. Growth studies on chitosan-amended media showed total inhibition of Poria placenta, Coriolus versicolor and Aspergillus niger using 1% w/v concentration.Chitosan with high average molecular weight (MW) was more efficient against mould and staining fungi than chitosan with low MW. Agar plate leaching tests showed only a small leaching effect using a 5% concentration on A. niger and P. placenta. Decay testing with P. placenta demonstrated efficacy using 5% and 2.5% concentrations in unleached samples. Leaching decreased the efficacy of chitosan and further investigations are needed to improve the fixation in wood.
Authors
Geir ØstrengAbstract
Artikkelen er basert på resultater fra et nordisk kurs i kalibrering av apparater for måling av CO2-frigivelse fra jord. Geir Østreng deltok på kurset med det apparatet som er brukt bl.a. i SIP Karbondynamikk i skogsjord 2000-2005, og han er medforfatter.
Authors
Inger Sundheim Fløistad Ketil KohmannAbstract
When spring frost occur on recently planted forest sites, severe damage may occur to the seedlings. The aim of the present study was to test how different levels of nutrient concentrations in the seedlings affected spring frost hardiness and time of bud break.Seedlings were grown in a greenhouse for one season and supplied with fertiliser containing 22, 43 and 72 mg N per litre respectively. The treatments resulted in needle nitrogen concentrations ranging from 0,9 to 1,8 % in the autumn.After winter storage at 0C, bud break was recorded on seedlings growing in greenhouse, outdoors and in growth chambers at 12C and at 17C. Freezing tests were performed on seedlings directly removed from winter storage and following one week growth in the greenhouse. Seedlings receiving fertiliser with 43 mg per litre had less freezing injury than the two other fertilisation treatments. The ealiest bud break occurred in seedlings receiving 72 mg per litre.
Abstract
Introduction: The objectives of the present study were to monitor H. annosum colonization rate (Hietala et al., 2003) and expression of host chitinases in clonal Norway spruce material with differing resistances. Transcript levels of three chitinases, representing classes I, II and IV, were monitored with real-time PCR.Material and MethodsInoculation experiment: Ramets of two 32 -year-old clones differing in resistance were employed as host material. Inoculation and wounding was performed. A rectangular strip containing phloem and cambium, with the inoculation site in the middle, was removed 3, 7 and 14 days after inoculation.Quantification of fungal colonizationMultiplex real-time PCR detection of host and pathogen DNA was performed (Hietala et al., 2003). Quantification of gene expression: Chitinase levels were monitored with Singleplex real-time PCR.Results and ConclusionsThe colonization profiles provided by the quantitative multiplex real-time PCR procedure (Hietala et al., 2003), when combined with spatial and temporal transcript profiling of 3 chitinases, provide a useful basis for identifying defense related genes, and for assessing their impact on pathogen colonization rates.Three days after inoculation, comparable colonization levels were observed in both clones in the area immediately adjacent to inoculation. Fourteen days after infection, pathogen colonization was restricted to the area immediately adjacent to the site of inoculation for the strong clone (589), but had progressed further into the host tissue in the weak (409) clone.Transcript levels of the class II and IV chitinases increased following wounding or inoculation, while the transcript level of the class I chitinase declined following these treatments. Transcript levels of the class II and class IV chitinases were higher in areas immediately adjacent to the inoculation site in 589 than in similar sites in 409 three days after inoculation, suggesting that the clones differ in the rate of chitinase-related signalperception.
Abstract
No abstract has been registered