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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2010

Abstract

In terrestrial ecotoxicology there is a serious lack of data for potential hazards posed by engineered nanoparticles (ENPs). This is partly due to complex interactions between ENPs and the soil matrix, but also to the lack of suitable toxicological endpoints in organisms that are exposed to ENPs in a relevant manner. Earthworms are recognized as key organisms in terrestrial ecosystems, but so far only physiological endpoints of low sensitivity have been used in ecotoxicity studies with ENPs. We exposed the earthworm Lumbricus terrestris to different concentrations (1-100 ppm) silver nanoparticles of two contrasting sizes under three different conditions; in water, in food and in soil. After exposure, we measured the impact on apoptosis in different tissues using two staining techniques to determine apoptosis; TUNEL and Apostain. A dose-dependent response was detected with respect to apoptotic activity in a range of tissues both at acute and sub-lethal concentrations (down to 4 mg kg soil-1). Comparing exposure in water and soil showed that reduced bioavailability in soil is reflected in the apoptotic response. Apoptosis thus seems to be a sensitive endpoint for detection of adverse effects of silver nanoparticles, and potentially a powerful tool for quantifying environmental hazards posed by ENPs.