Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2010
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Anette Moldestad Ellen Mosleth Færgestad Bernt Hoel Anne Kjersti UhlenAbstract
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Ellen Mosleth Færgestad Anette Moldestad Bernt Hoel Arne Oddvar Skjelvåg Anne Kjersti UhlenAbstract
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Development of ontogenic resistance to powdery mildew (Podosphaera aphanis) on strawberry leaves has been reported, however, the components of resistance have not been elucidated. Five developmental stages of strawberry leaves were identified and assigned numerical values from newly emerged and unexpanded (S1) to fully expanded and dark green (S5) of cvs. Korona and Senga Sengana. The upper and lower surface of the leaves were inoculated from each of the five leaf developmental stages and incubated under controlled conditions. The effect of leaf age on germination, infection efficiency, latency period, and sporulation were later evaluated. All responses were significantly (p = 0.05) affected by leaf age. Germination percentage, infection efficiency, and sporulation were highest, and latent periods were shortest on S1 leaves of both cultivars. On Senga Sengana, germinating conidia produced fewer secondary hyphae during infection. Conidia produced very few secondary hyphae and did not sporulate on S3 leaves, and no infections established on S4 or S5 leaves. The high success of infection and colonization of P. aphanis on S1 leaves indicates that disease is established preferentially on emergent and expanding leaves and these should be the target of management strategies.
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Authors
Hsin-Ho Huang Daniel Camsund Peter Lindblad Thorsten HeidornAbstract
Cyanobacteria are suitable for sustainable, solar-powered biotechnological applications. Synthetic biology connects biology with computational design and an engineering perspective, but requires efficient tools and information about the function of biological parts and systems. To enable the development of cyanobacterial Synthetic Biology, several molecular tools were developed and characterized: (i) a broad-host-range BioBrick shuttle vector, pPMQAK1, was constructed and confirmed to replicate in Escherichia coli and three different cyanobacterial strains. (ii) The fluorescent proteins Cerulean, GFPmut3B and EYFP have been demonstrated to work as reporter proteins in cyanobacteria, in spite of the strong background of photosynthetic pigments. (iii) Several promoters, like P(rnpB) and variants of P(rbcL), and a version of the promoter P(trc) with two operators for enhanced repression, were developed and characterized in Synechocystis sp. strain PCC6803. (iv) It was shown that a system for targeted protein degradation, which is needed to enable dynamic expression studies, is working in Synechocystis sp. strain PCC6803. The pPMQAK1 shuttle vector allows the use of the growing numbers of BioBrick parts in many prokaryotes, and the other tools herein implemented facilitate the development of new parts and systems in cyanobacteria.
Authors
Arild Andersen Trond Hofsvang May Bente Brurberg Christer Magnusson Trond Rafoss Brita Toppe Anne Marte Tronsmo Bjørn Økland Leif SundheimAbstract
No abstract has been registered