Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2010
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Authors
Hsin-Ho Huang Daniel Camsund Peter Lindblad Thorsten HeidornAbstract
Cyanobacteria are suitable for sustainable, solar-powered biotechnological applications. Synthetic biology connects biology with computational design and an engineering perspective, but requires efficient tools and information about the function of biological parts and systems. To enable the development of cyanobacterial Synthetic Biology, several molecular tools were developed and characterized: (i) a broad-host-range BioBrick shuttle vector, pPMQAK1, was constructed and confirmed to replicate in Escherichia coli and three different cyanobacterial strains. (ii) The fluorescent proteins Cerulean, GFPmut3B and EYFP have been demonstrated to work as reporter proteins in cyanobacteria, in spite of the strong background of photosynthetic pigments. (iii) Several promoters, like P(rnpB) and variants of P(rbcL), and a version of the promoter P(trc) with two operators for enhanced repression, were developed and characterized in Synechocystis sp. strain PCC6803. (iv) It was shown that a system for targeted protein degradation, which is needed to enable dynamic expression studies, is working in Synechocystis sp. strain PCC6803. The pPMQAK1 shuttle vector allows the use of the growing numbers of BioBrick parts in many prokaryotes, and the other tools herein implemented facilitate the development of new parts and systems in cyanobacteria.
Authors
Arild Andersen Trond Hofsvang May Bente Brurberg Christer Magnusson Trond Rafoss Brita Toppe Anne Marte Tronsmo Bjørn Økland Leif SundheimAbstract
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Authors
Arild Andersen Trond Hofsvang May Bente Brurberg Christer Magnusson Trond Rafoss Brita Toppe Anne Marte Tronsmo Bjørn Økland Leif SundheimAbstract
No abstract has been registered
Authors
Arild Andersen Trond Hofsvang May Bente Brurberg Christer Magnusson Trond Rafoss Anne Marte Tronsmo Bjørn Økland Leif SundheimAbstract
No abstract has been registered
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Authors
Ana Maria Barata da Silva Åsmund Asdal Elinor LipmanAbstract
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Authors
Mari Mette Tollefsrud Christoph SperisenAbstract
Norway spruce (Picea abies [L.] Karst.), one of the most important species of the boreal forest, is found naturally distributed in two disjunct ranges; one northern and one southern European range. These ranges have been shown to correspond to two genetically distinct lineages. In this talk, results on the genetic structure based on mitochondrial DNA (mtDNA; nad1) and nuclear microsatellites in populations from the northern European lineage, will be presented and discussed in the light of its glacial and postglacial history. The genetic relationship between Norway spruce and its will closest relative Siberian spruce (Picea abovata Lebed.) will also be discussed based on results from mtDNA and paternally inherited chloroplast DNA (cpDNA). Genetic structure of northern European Norway spruce is generally shallow, consistent with recently compiled pollen data, suggesting that Norway spruce in Northern Europe was colonized from a single Russian refugium. Despite the low differentiation found, the structure at both mtDNA and nuclear DNA suggest that expansion westwards took place along two main migration routes; one northwestern over Finland to northern Scandinavia, and one southwestern across the Baltic Sea into Scandinavia. Based on both mtDNA and nuclear DNA, populations in the oldest regions of Russia and the Baltic States show the highest diversity. Based on pollen data, colonization of these regions took place at high population densities, helping to maintain high levels of diversity. Also populations in southern Sweden and southern Norway show relatively high levels of diversity compared to the more northern Scandinavian populations. This may be due to the additional southern migration route into the region, as well as pollen-mediated gene flow in the south which seems to efficiently have replenished the loss of nuclear diversity following postglacial colonization. In the northern part of Fennoscandia, smaller effective population size due to more limited seed and pollen production may have caused decreased nuclear diversity and increased inbreeding, reflecting the ecological marginality of the species in the north. Genetic differentiation between Norway spruce and Siberian spruce based on mitochondrial and chloroplast markers suggest that the border between the two species occur east of the Ural Mountain, following the river Ob. Still, the paternally inherited cpDNA marker suggests extensive introgression from Siberian spruce into the northern European range of Norway spruce. Introgression via pollen may thus acts as a mechanism of dispersal of Siberian spruce genes into the northern European gene pool of Norway spruce.