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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2016

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Abstract

Dothistroma needle blight (DNB) is one of the most important diseases of pine. Although its notoriety stems from Southern Hemisphere epidemics in Pinus radiata plantations, the disease has increased in prevalence and severity in areas of the Northern Hemisphere, including Europe, during the last two decades. This increase has largely been attributed to expanded planting of susceptible hosts, anthropogenic dispersal of the causative pathogens and changes in climate conducive to disease development. The last comprehensive review of DNB was published in 2004, with updates on geographic distribution and host species in 2009. Importantly, the recognition that two species, Dothistroma septosporum and D. pini, cause DNB emerged only relatively recently in 2004. These two species are morphologically very similar, and DNA-based techniques are needed to distinguish between them. Consequently, many records of host species affected or geographic location of DNB prior to 2004 are inconclusive or even misleading. The objectives of this review were (i) to provide a new database in which detailed records of DNB from 62 countries are collated; (ii) to chart the current global distribution of D. septosporum and D. pini; (iii) to list all known host species and to consider their susceptibility globally; (iv) to collate the published results of provenance trials; and (v) to consider the effects of site factors on disease incidence and severity. The review shows that DNB occurs in 76 countries, with D. septosporum confirmed to occur in 44 and D. pini in 13. There are now 109 documented Pinaceae host taxa for Dothistroma species, spanning six genera (Abies, Cedrus, Larix, Picea, Pinus and Pseudotsuga), with Pinus being the dominant host genus, accounting for 95 host taxa. The relative susceptibilities of these hosts to Dothistroma species are reported, providing a resource to inform species choice in forest planting. Country records show that most DNB outbreaks in Europe occur on Pinus nigra and its subspecies. It is anticipated that the collaborative work described in this review will both underpin a broader global research strategy to manage DNB in the future and provide a model for the study of other forest pathogens.

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Abstract

Lack of resistance to pink snow mould (Microdochium nivale) is a major constraint for adaptation of perennial ryegrass (Lolium perenne L.) to continental regions with long-lasting snow cover at higher latitudes. Almost all investigations of genetic variation in resistance have been performed using cold acclimated plants. However, there may be variation in resistance mechanisms that are functioning independently of cold acclimation. In this study our aim was to identify candidate genes involved in such resistance mechanisms. We first characterized variation in resistance to M. nivale among non-acclimated genotypes from the Norwegian cultivar ‘Fagerlin’ based on relative regrowth and fungal quantification by real-time qPCR. One resistant and one susceptible genotype were selected for transcriptome analysis using paired-end sequencing by Illumina Hiseq 2000. Transcriptome profiles, GO enrichment and KEGG pathway analysis indicate that defense response related genes are differentially expressed between the resistant and the susceptible genotype. A significant up-regulation of defense related genes, as well as genes involved in cell wall cellulose metabolic processes and aryl-alcohol dehydrogenase (NADP+) activity, was observed in the resistant genotype. The candidate genes identified in this study might be potential molecular marker resources for breeding perennial ryegrass cultivars with improved resistance to pink snow mould.

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Abstract

A glutathione (GSH) adduct of the mycotoxin 4-deoxynivalenol (DON), together with a range of related conjugates, has recently been tentatively identified by LC-MS of DON-treated wheat spikelets. In this study, we prepared samples of DON conjugated at the 10- and 13-positions with GSH, Cys, CysGly, -GluCys and N-acetylcysteine (NAC). The mixtures of conjugates were used as standards for LC-HRMS analysis of one of the DON-treated wheat spikelet samples, as well as 19 Norwegian grain samples of spring wheat and 16 grain samples of oats that were naturally-contaminated with DON at concentrations higher than 1 mg/kg. The artificially-contaminated wheat spikelets contained conjugates of GSH, CysGly and Cys coupled at the olefinic 10-position of DON, whereas the naturally-contaminated harvest-ripe grain samples contained GSH, CysGly, Cys, and NAC coupled mainly at the 13-position on the epoxy group. The identities of the conjugates were confirmed by LC-HRMS comparison with authentic standards, oxidation to the sulfoxides with hydrogen peroxide, and examination of product-ion spectra from LC-HRMS/MS analysis. No -GluCys adducts of DON were detected in any of the samples. The presence of 15-O-acetyl-DON was demonstrated for the first time in Norwegian grain. The results indicate that a small but significant proportion of DON is metabolized via the GSH-conjugation pathway in plants. To our knowledge, this is the first report of in vivo conjugation of trichothecenes via their epoxy group, which has generally been viewed as unreactive. Because conjugation at the 13-position of DON and other trichothecenes has been shown to be irreversible, this type of conjugate may prove useful as a biomarker of exposure to DON and other 12,13-epoxytrichothecenes.

Abstract

Grassland and the associated ruminant livestock production is the backbone of Norwegian agriculture, as ruminant products contribute nearly 50% of the gross income of the agricultural sector. About 2/3 of the agricultural area is used for temporary and permanent grassland, and a large proportion (40%) of the arable land is used for producing cereals that are included in concentrate mixtures fed to ruminants. The huge variation in climatic conditions, caused by the wide range in both latitude and altitude as well as in the distance to the coast, determines the land use and choice of species and varieties used in grassland. However, the dominating ley grassland species used in in almost all parts of the country are timothy (Phleum pratense L.), meadow fescue (Festuca pratensis Huds.) and red clover (Trifolium pratense L.). The use of perennial ryegrass (Lolium perenne L.) is increasing, particularly in the southwestern parts of the country. The grassland yields and forage feed quality have remained very much the same during the last decade, while the intensity in ruminant production, e.g. milk yield per cow, has increased considerably. Factors that have contributed to grassland yield stagnation are probably the increasing proportion of rented farmland by larger farm units, the increasing price ratio between livestock products and feed concentrates and the increasing cost of producing forage relative to the price of concentrates

Abstract

Grassland and the associated ruminant livestock production is the backbone of Norwegian agriculture, as ruminant products contribute nearly 50% of the gross income of the agricultural sector. About 2/3 of the agricultural area is used for temporary and permanent grassland, and a large proportion (40%) of the arable land is used for producing cereals that are included in concentrate mixtures fed to ruminants. The huge variation in climatic conditions, caused by the wide range in both latitude and altitude as well as in the distance to the coast, determines the land use and choice of species and varieties used in grassland. However, the dominating ley grassland species used in in almost all parts of the country are timothy (Phleum pratense L.), meadow fescue (Festuca pratensis Huds.) and red clover (Trifolium pratense L.). The use of perennial ryegrass (Lolium perenne L.) is increasing, particularly in the southwestern parts of the country. The grassland yields and forage feed quality have remained very much the same during the last decade, while the intensity in ruminant production, e.g. milk yield per cow, has increased considerably. Factors that have contributed to grassland yield stagnation are probably the increasing proportion of rented farmland by larger farm units, the increasing price ratio between livestock products and feed concentrates and the increasing cost of producing forage relative to the price of concentrates

Abstract

NIBIO – the Norwegian Institute of Bioeconomy Research - develops knowledge on what the Norwegian bioeconomy actually is. NIBIO has a strong focus on sustainability, value creation and the primary industries’ knowledge requirements. At the same time, the environment, resource base and climate are core areas in our work. This gives us a unique combination of expertise, with which we can integrate questions of production with environmental challenges, and where economic and social dimensions are included. Basic knowledge of biology and biological processes linked to advanced technology provides us with opportunities for new understanding, new solutions and increased value creation. It is important for NIBIO that our knowledge is of use for industry, the administration, decision makers and other research institutions. NIBIO - Norwegian Institute of Bioeconomy Research was established July 1 2015 as a merger between the Norwegian Institute for Agricultural and Environmental Research, the Norwegian Agricultural Economics Research Institute and Norwegian Forest and Landscape Institute. In this brochure we present a taste of the projects we have worked on in NIBO’s first year, examples that show the institute’s breadth and potential.