Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2012
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Jihong Liu ClarkeAbstract
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Ragnar TveteråsAbstract
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Simon WeldonAbstract
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Frans van den Berg A. Tiktak G. B. M. Heuvelink S. L. G. E. Burgers D. J. Brus F. de Vries Jannes Stolte J. G. KroesAbstract
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Light-emitting diodes (LEDs), high-pressure sodium lamps (HPSLs) and some cladding materials offer possibilities of influencing arthropod integrated pest management in greenhouse crops where light quality, quantity and photoperiod differ from nature. Light intensity, photoperiod and wavelength distribution affect plant functions and quality which, in turn, can be reflected in the performance of herbivores. The attenuation of UV-light in HPSL spectrum and in the natural winter daylight of northern latitudes may make plants more vulnerable to pests, whereas the high ratio of red to far-red of HPSLs may act to compensate for the effects of attenuated UV-levels. High red to far red ratio has been shown to result in increased production of plant phenolics and physical defences such as leaf toughness, which, in turn, can negatively influence the performance of some herbivore guilds on plants. Specific spectra produced by LEDs can influence plant quality and hence herbivore performance, but direct effects on arthropods can be even more pronounced, such as the inability to visually locat host plants in red and blue lights. Other direct effects of artificial light on pests and beneficial organisms include the detrimental effect of UV-C and UV-B on arthropods, diapause prevention by species-specific wavelengths or photoperiods, attraction to yellow-green and polarized light, reduced visibility of host or prey, and changes in take-off behaviour. Other effects include response to light intensity, interactive effects of light quality and photoperiod on fecundity, and species-specific effects of continuous light on the population growth of arthropods and plant-infesting fungi as well as the rhythmic expression of xenobiotic metabolising genes in arthropods. The potential of using the knowledge of photobiology and visual ecology of organisms for plant protection are discussed using whiteflies and fungal diseases of plants as the model species.
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Gage Koehler Robert Wilson John V. Goodpaster Anita Sønsteby Xianyin Lai Frank A. Witzmann Jin-Sam You Jens Rohloff Stephen K, Randall Muath Alsheikh-YousefAbstract
To gain insight into the molecular basis contributing to overwintering hardiness, a comprehensive proteomic analysis comparing crowns of Fragaria × ananassa (octoploid strawberry) cultivars that differ in freezing tolerance was conducted. Four cultivars were examined for freeze tolerance and the most cold-tolerant cultivar (‘Jonsok’) and least tolerant cultivar (‘Frida’) were compared with a goal to reveal how freezing tolerance is achieved in this distinctive overwintering structure and to identify potential cold-tolerance associated biomarkers. Supported by univariate and multivariate analysis, a total of 63 spots from 2DE analysis and 135 proteins from label-free quantitative proteomics (LFQP) were identified as significantly differentially expressed in crown tissue from the two strawberry cultivars exposed to 0, 2, and 42 day cold treatment. Proteins identified as cold tolerance associated included molecular chaperones, antioxidants/detoxifying enzymes, metabolic enzymes, pathogenesis related proteins and flavonoid pathway proteins. A number of proteins were newly identified as associated with cold tolerance. Distinctive mechanisms for cold tolerance were characterized for two cultivars. In particular, the ‘Frida’ cold response emphasized proteins specific to flavonoid biosynthesis, while the more freezing tolerant ‘Jonsok’ had a more comprehensive suite of known stress responsive proteins including those involved in antioxidation, detoxification, and disease resistance. The molecular basis for ‘Jonsok’ enhanced cold tolerance can be explained by the constitutive level of a number of proteins that provide a physiological stress-tolerant “poise”.
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