Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2017
Authors
Yuka Kojima Aniko Varnai Takuya Ishida Naoki Sunagawa Dejan Petrovic Kiyohiko Igarashi Jody Jellison Barry Goodell Gry Alfredsen Bjørge Westereng Vincent Eijsink Makoto YoshidaAbstract
No abstract has been registered
Authors
Franck Anicet Ditengou Dulceneia Gomes Hugues Nziengui Philip Kochersperger Hanna Lasok Violante Medeiros Ivan Paponov Szilvia Krisztina Nagy Timea Virag Nadai Tamas Meszaros Beata Barnabas Beata Izabela Ditengou Katja Rapp Linlin Qi Xugang Li Claude Becker Chuanyou Li Robert Doczi Klaus PalmeAbstract
Auxin gradients are sustained by series of influx and efflux carriers whose subcellular local- ization is sensitive to both exogenous and endogenous factors. Recently the localization of the Arabidopsis thaliana auxin efflux carrier PIN-FORMED (PIN) 6 was reported to be tissue- specific and regulated through unknown mechanisms. Here, we used genetic, molecular and pharmacological approaches to characterize the molecular mechanism(s) controlling the subcellular localization of PIN6. PIN6 localizes to endomembrane domains in tissues with low PIN6 expression levels such as roots, but localizes at the plasma membrane (PM) in tissues with increased PIN6 expression such as the inflorescence stem and nectary glands. We provide evidence that this dual local- ization is controlled by PIN6 phosphorylation and demonstrate that PIN6 is phosphorylated by mitogen-activated protein kinases (MAPKs) MPK4 and MPK6. The analysis of transgenic plants expressing PIN6 at PM or in endomembrane domains reveals that PIN6 subcellular localization is critical for Arabidopsis inflorescence stem elongation post-flowering (bolting). In line with a role for PIN6 in plant bolting, inflorescence stems elongate faster in pin6 mutant plants than in wild-type plants. We propose that PIN6 subcellular localization is under the control of developmental signals acting on tissue-specific determinants controlling PIN6-expression levels and PIN6 phosphorylation.
Authors
Merete Wiken Dees Renata Lebecka Juliana Perminow Robert Czajkowski Anna Grupa Agata Motyka Sabina Zoledowska Jadwiga Śliwka Ewa Lojkowska May Bente BrurbergAbstract
Soft rot and blackleg of potato caused by pectinolytic bacteria lead to severe economic losses in potato production worldwide. To investigate the species composition of bacteria causing soft rot and black leg of potato in Norway and Poland, bacteria were isolated from potato tubers and stems. Forty-one Norwegian strains and 42 Polish strains that formed cavities on pectate medium were selected for potato tuber maceration assays and sequencing of three housekeeping genes (dnaX, icdA and mdh) for species identification and phylogenetic analysis. The distribution of the species causing soft rot and blackleg in Norway and Poland differed: we have demonstrated that mainly P. atrosepticum and P. c. subsp. carotovorum are the causal agents of soft rot and blackleg of potatoes in Norway, while P. wasabiae was identified as one of the most important soft rot pathogens in Poland. In contrast to the other European countries, D. solani seem not to be a major pathogen of potato in Norway and Poland. The Norwegian and Polish P. c. subsp. carotovorum and P. wasabiae strains did not cluster with type strains of the respective species in the phylogenetic analysis, which underlines the taxonomic complexity of the genus Pectobacterium. No correlation between the country of origin and clustering of the strains was observed. All strains tested in this study were able to macerate potato tissue. The ability to macerate potato tissue was significantly greater for the P. c. subsp. carotovorum and Dickeya spp., compared to P. atrosepticum and P. wasabiae.
Authors
Greeley Beck Emil Engelund Thybring Lisbeth Garbrecht Thygesen Callum Aidan Stephen HillAbstract
No abstract has been registered
Authors
Nicholas ClarkeAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Jihong Liu ClarkeAbstract
No abstract has been registered
Authors
Ola Stedje Hanserud Francesco Cherubini Anne Falk Øgaard Daniel Beat Mueller Helge BrattebøAbstract
Increased nutrient cycling in the agri-food system is a way to achieve a healthier nutrient stewardship and more sustainable food production. In life cycle assessment (LCA) studies, use of recycled fertilizer products is often credited by the substitution method, which subtracts the environmental burdens associated with avoided production of mineral fertilizer from the system under study. The environmental benefits from avoided fertilizer production can make an important contribution to the results, but different calculation principles and often implicit assumptions are used to estimate the amount of avoided mineral fertilizer. This may hinder comparisons between studies. The present study therefore examines how the choice of substitution principles influences LCA results. Three different substitution principles, called one-to-one, maintenance, and adjusted maintenance, are identified, and we test the importance of these in a case study on cattle slurry management. We show that the inventory of avoided mineral fertilizer varies greatly when the different principles are applied, with strong influences on two-thirds of LCA impact categories. With the one-to-one principle, there is a risk of systematically over-estimating the environmental benefits from nutrient cycling. In a sensitivity analysis we show that the difference between the principles is closely related to the application rate and levels of residual nutrients in the soil. We recommend that LCA practitioners first and foremost state and justify the substitution method they use, in order to increase transparency and comparability with other studies. © 2017 Elsevier B.V. All rights reserved.
Authors
Rasmus Dam Wollenberg Wagma Saei Klaus Ringsborg Westphal Carina Sloth Klitgaard Kåre Lehmann Nielsen Erik Lysøe Donald Max Gardiner Reinhard Wimmer Teis Esben Sondergaard Jens Laurids SørensenAbstract
Production of chrysogine has been reported from several fungal genera including Penicillium, Aspergillus, and Fusarium. Anthranilic acid and pyruvic acid, which are expected precursors of chrysogine, enhance production of this compound. A possible route for the biosynthesis using these substrates is via a nonribosomal peptide synthetase (NRPS). Through comparative analysis of the NRPSs from genome-sequenced producers of chrysogine we identified a candidate NRPS cluster comprising five additional genes named chry2–6. Deletion of the two-module NRPS (NRPS14 = chry1) abolished chrysogine production in Fusarium graminearum, indicating that the gene cluster is responsible for chrysogine biosynthesis. Overexpression of NRPS14 enhanced chrysogine production, suggesting that the NRPS is the bottleneck in the biosynthetic pathway.
Abstract
In this paper, we examine citizen and consumer attitudes towards, and preferences for, private and public goods from organic agriculture in Norway. The study is based on a survey among 939 Norwegians. The results show that in the role as citizens, the respondents hold a moderate belief in the superiority of organic farming concerning the production of public goods, but they give relatively low priority to prompting organic farming compared to other agricultural policy goals. In the role as consumers (choice experiment), the respondents were willing to pay for several attributes of organic food. Only 6% of the respondents buy organic food as often as they can. The most important reasons for buying organic food are health and environmental concerns, while animal welfare has little importance. Lack of perceived superiority regarding health benefits, taste, safety and environment are important reasons for not consuming (more) organic food among those who rarely or never buy organic food.