Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2018
Authors
Olav Aaseth Hegnar Dejan Petrovic Bastien Bissaro Gry Alfredsen Aniko Varnai Vincent EijsinkAbstract
Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that perform oxidative cleavage of recalcitrant polysaccharides. We have purified and characterized a recombinant family AA9 LPMO, LPMO9B, from Gloeophyllum trabeum (GtLPMO9B) which is active on both cellulose and xyloglucan. Activity of the enzyme was tested in the presence of three different reductants: ascorbic acid, gallic acid, and 2,3-dihydroxybenzoic acid (2,3-DHBA). Under standard aerobic conditions typically used in LPMO experiments, the first two reductants could drive LPMO catalysis whereas 2,3-DHBA could not. In agreement with the recent discovery that H2O2 can drive LPMO catalysis, we show that gradual addition of H2O2 allowed LPMO activity at very low, substoichiometric (relative to products formed) reductant concentrations. Most importantly, we found that while 2,3-DHBA is not capable of driving the LPMO reaction under standard aerobic conditions, it can do so in the presence of externally added H2O2. At alkaline pH, 2,3-DHBA is able to drive the LPMO reaction without externally added H2O2, and this ability overlaps entirely the endogenous generation of H2O2 by GtLPMO9B-catalyzed oxidation of 2,3-DHBA. These findings support the notion that H2O2 is a cosubstrate of LPMOs and provide insight into how LPMO reactions depend on, and may be controlled by, the choice of pH and reductant.
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No abstract has been registered
Authors
Anita SønstebyAbstract
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Authors
Anita SønstebyAbstract
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Authors
Anita SønstebyAbstract
No abstract has been registered
Authors
Anita SønstebyAbstract
No abstract has been registered
Authors
Anna Avetisyan Anush Panosyan Martina Paponov Ivan Paponov Inger Martinussen Kirsten Krause Zara Harutyunyan Irina Vardanian Andreas Melikyan Manvel Badalyan Tatevik Aloyan Alla Vardanyan Samvel Shoukourian Laura JaakolaAbstract
No abstract has been registered
Authors
Inger Martinussen Tomasz Leszek Woznicki Unni Myrheim Roos Anita Sønsteby Eivind Uleberg Anne Linn HykkerudAbstract
No abstract has been registered
Abstract
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Authors
Caroline Brophy John A. Finn Andreas Lüscher M. Suter Laura Kirwan Maria-Teresa Sebastià Aslaug Helgadottir Ole Hans Baadshaug G. Bélanger Alistair Black Rosemary P. Collins Jure Čop Sigridur Dalmannsdottir Ignacio Delgado Anjo Elgersma Mick Fothergill Bodil E. Frankow-Lindberg An Ghesquiere Barbara Golinska Piotr Golinski Philippe Grieu Anne-Maj Gustavsson Mats Höglind Olivier Huguenin-Elie Marit Jørgensen Zydre Kadziuliene Päivi Kurki Rosa Llurba Tor Lunnan Claudio Porqueddu Ulrich Thumm John ConnollyAbstract
No abstract has been registered