Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2022
Authors
Diogo N. Cosenza Petteri Packalén Matti Maltamo Petri Varvia Janne Räty Paula Soares Margarida Tomé Jacob L. Strunk Lauri KorhonenAbstract
Semi- and nonparametric models are popular in the area-based approach (ABA) using airborne laser scanning. It is unclear, however, how many predictors and training plots are needed to provide accurate predictions without overfitting. This work aims to explore these limits for various approaches: ordinary least squares regression (OLS), generalized additive models (GAM), least absolute shrinkage and selection operator (LASSO), random forest (RF), support vector machine (SVM), and Gaussian process regression (GPR). We modeled timber volume (m3·ha–1) for four boreal sites using ABA with 2–39 predictors and 20–500 training plots. OLS, GAM, LASSO, and SVM overfitted as the number of predictors approached the number of training plots. They required ≥15 plots per predictor to provide accurate predictions (RMSE ≤30%). GAM required ≥250 plots regardless of the number of predictors. The number of predictors only mildly affected RF and GPR, but they required ≥200 and ≥250 training plots, respectively. RF did not overfit in any circumstances, whereas GPR overfit even with 500 training plots. Overall, using up to 39 predictors did not generally result in overfit, and for most model types, it resulted in better accuracy for sufficiently large datasets (≥250 plots).
Authors
Randi Seljåsen Barbara Kusznierewicz Agnieszka Bartoszek Jørgen A.B. Mølmann Ingunn M. VågenAbstract
Biosynthesis of phytochemicals in leaves of Brassica can be initiated by abiotic factors. The aim of the study was to investigate elicitor treatments to add value to waste of cabbage. A leaf waste fraction from industrial trimming of head cabbage was exposed to UV radiation (250–400 nm, 59 and 99 kJ∙m−2, respectively), photosynthetic active radiation (PAR, 400–700 nm, 497 kJ∙m−2), and ultrasound in water bath (35 kHz, at 15, 30 and 61 kJ∙l−1 water), in order to improve nutraceutical concentration. UV was more effective than PAR to increase the level of flavonols (2 to 3-fold higher) and hydroxycinnamate monosaccharides (1 to 10-fold higher). PAR was three times as effective as UV to increase anthocyanins. Interaction of PAR + UV increased antioxidant activity (30%), the content of five phenolics (1.4 to 10-fold higher), and hydroxycinnamic monosaccharides (compared with PAR or UV alone). Indoles were reduced (40–52%) by UV, but the other glucosinolates (GLS) were unaffected. Ultrasound did not influence any parameters. The results are important for white cabbage by-products by demonstrating that UV + PAR can be successfully used as an effectual tool to increase important phenolics and antioxidant activity of waste fraction leaves without an adverse effect on the main GLS.
Abstract
No abstract has been registered
Authors
Ercan Yildiz Mehmet Yaman Sezai Ercisli Ahmet Sumbul Osman Sonmez Adem Gunes Mehmet Ramazan Bozhuyuk Darius KviklysAbstract
The plant pomological characteristics and physiological behaviors of genotypes in modern apple cultivation could be different depending on the use of rootstock, changing growth ecology and application of biological control agents. The aim of this research was to determine the effects of rhizobacteria application on leaf and fruit nutrient contents in different apple scion–rootstock combinations. This study was carried out with seven standard cultivars (Scarlet Spur, Red Chief, Fuji, Jeromine, Galaxy Gala, Granny Smith, and Golden Reinders) budded on M.9 and MM.106 rootstocks. In the experiment, trees were sprayed by a nitrogen + phosphorus solvent rhizobacteria three times, with an interval of 15 days in the spring period. The effect of rhizobacteria application on leaf and fruit nutrient contents was statistically significant and provided generally significant positive contributions, except for leaf Mg content. Comparing both rootstocks, the positive effect of bacterial application was higher on the M.9 rootstock for leaf N and B content and fruit N and Fe content, and on the MM.106 rootstock for other nutrient content. While the effects of bacterial application on the basis of cultivars were generally positive, the highest positive contribution was made in leaf P content (10.7%) and fruit Mn content (32.1%) of the Fuji cultivar. Considering the total increase in nutrients in scion–rootstocks combination, rhizobacteria application had a positive effect on the leaf nutrient contents in Golden Reinders/MM.106, but not leaf K content. The highest increases in leaves of scion-rootstock combinations were determined as 4.0% in N content in Granny Smith/M.9, 14.1% in P content in Scarlet Spur/MM.106, 7.1% in K content in Fuji/MM.106, 4.4% in Ca content in Jeromine/M.9, and 14.0% in Mg content in Granny Smith/MM.106. The highest increase in fruit nutrient contents was between 4.9% (N content) and 13.5% (Ca content) for macro elements, and between 9.5% (Cu content) and 41.8% (Mn content) for microelements. The results of the present study may provide significant leads for further studies on this subject.
Abstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Marie-Therese Krieger Julia Ditton Harald Albrecht Luise Linderl Johannes Kollmann Leonardo H. TeixeiraAbstract
Changes in grassland management lead to alterations in community structure and can facilitate rapid expansion of both non-native and native invaders. Light availability differs greatly depending on grassland density, and competition for light is an important component of species dynamics. In this study, we examined if light reduction is an effective method to suppress a native invader in pre-alpine meadows of low to moderate land-use intensity. Our study focused on the effects of shading and other site conditions on vegetative and generative growth of Jacobaea aquatica, a poisonous hemicryptophyte regionally spreading in C Europe. We hypothesized that negative shade effects occur in addition to suppression by high grassland productivity, moist climate and less intense management. Furthermore, we postulated that shading affects vegetative growth more than reproduction. To understand the effects of shading we conducted a greenhouse experiment with plants grown under different shading nets. These results were compared to data gathered from 20 field sites that represented a distinct gradient in grassland management and shading. Overall, performance of generative J. aquatica plants was reduced by shading in the greenhouse, while density of vegetative plants was reduced in the field. In the greenhouse, plants affected by shading had significantly fewer flower heads and slightly smaller rosettes. Under field conditions, shading effects occurred together with additional environmental factors, while density of vegetative plants was significantly reduced by shading. Our data show that while realising high shading effects in the field is hard to accomplish, light reduction still has an influence on plant performance and population density, and could therefore be used to suppress the invasive native J. aquatica. In low to moderate intensity grasslands, suppression can be achieved by delaying the first mowing, thus enhancing shading. We conclude that manipulating environmental filters to increase resource competition is recommended as an alternative management tool to control the abundance of invasive native plants in grassland.
Abstract
Three strains of chlorophyte microalgae indigenous in Norway were studied regarding their potential for nutrient removal and resource recovery from wastewater. The nutrient uptake, growth, and cell composition (total proteins and carbohydrates) were monitored under a controlled batch environment for 14 days. Additionally, the fatty acids were analyzed at the end of the study. The fastest nutrient removal was achieved by Lobochlamys segnis F12 that used up NH4+ (28 mg L-1) and PO43- (15 mg L-1) after 4 days. Similar PO43- uptake was achieved by Tetradesmus wisconsinensis H1 while its NH4+ uptake took 2 days longer. Both strains showed a higher specific growth rate (1.1 day-1) than Klebsormidium flaccidum NIVA-CHL80 (0.55 day-1). The highest biomass (1.276 ± 21 mg L-1) and carbohydrates content (40%) were achieved by T. wisconsinensis. K. flaccidum was characterized by superior protein content (53 ± 4%). In terms of total fatty acids production both K. flaccidum and L. segnis were favored (184 ± 6 and 193 ± 12 mg g-1 dry cells), especially with their high polyunsaturated fatty acid content (82 and 67%, respectively). The fatty acids of K. flaccidum consisted mainly C18:2 n-6 (73% of the total). L. segnis had a preferable n3 to n6 ratio (1.3) in their fatty acid profile. The proteins and carbohydrates content changed in all strains depending on the growth stage. Therefore, resource recovery scenarios could be further optimized for a specific cell component production combined with an appropriate strategy for nutrient removal from wastewater.
Authors
Ana-Maria Pantazica Mihaela-Olivia Dobrica Catalin Lazar Cristina Scurtu Catalin Tucureanu Iuliana Caras Irina Ionescu Adriana Costache Adrian Onu Jihong Liu Clarke Crina Stavaru Norica Branza-NichitaAbstract
Despite the availability of improved antiviral therapies, infection with Hepatitis B virus (HBV) remains a3 significant health issue, as a curable treatment is yet to be discovered. Current HBV vaccines relaying on the efficient expression of the small (S) envelope protein in yeast and the implementation of mass vaccination programs have clearly contributed to containment of the disease. However, the lack of an efficient immune response in up to 10% of vaccinated adults, the controversies regarding the seroprotection persistence in vaccine responders and the emergence of vaccine escape virus mutations urge for the development of better HBV immunogens. Due to the critical role played by the preS1 domain of the large (L) envelope protein in HBV infection and its ability to trigger virus neutralizing antibodies, including this protein in novel vaccine formulations has been considered a promising strategy to overcome the limitations of S only-based vaccines. In this work we aimed to combine relevant L and S epitopes in chimeric antigens, by inserting preS1 sequences within the external antigenic loop of S, followed by production in mammalian cells and detailed analysis of their antigenic and immunogenic properties. Of the newly designed antigens, the S/preS116–42 protein assembled in subviral particles (SVP) showed the highest expression and secretion levels, therefore, it was selected for further studies in vivo. Analysis of the immune response induced in mice vaccinated with S/preS116–42- and S-SVPs, respectively, demonstrated enhanced immunogenicity of the former and its ability to activate both humoral and cellular immune responses. This combined activation resulted in production of neutralizing antibodies against both wild-type and vaccine-escape HBV variants. Our results validate the design of chimeric HBV antigens and promote the novel S/preS1 protein as a potential vaccine candidate for administration in poor-responders to current HBV vaccines.
Authors
Kamilla SkaalsveenAbstract
No abstract has been registered