Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2015
Authors
Gage Koehler Jens Rohloff Robert Charles Wilson Joachim Kopka Alexander Erban Per Winge Atle M. Bones Jahn Davik Muath K Alsheikh Stephen K. RandallAbstract
To assess underlying metabolic processes and regulatory mechanisms during cold exposure of strawberry, integrative “omic” approaches were applied to F. ananassa ‘Korona’. Analysis of leaf tissue was emphasized because of its immediate and early responses in the cold acclimation processes. Levels of metabolites, proteins, and transcripts in tissues from plants grown at 20°C were compared to those following 1 to 10 days of cold exposure. When leaves and roots were subjected to GC/TOF-MS-based metabolite profiling, about 160 compounds comprising mostly structurally annotated primary metabolites and secondary metabolites, were found. Overall, ‘Korona’ showed a modest increase of protective metabolites such as amino acids (aspartic acid, leucine, isoleucine, and valine), pentoses, phosphorylated and non-phosphorylated hexoses, and distinct compounds of the raffinose pathway (galactinol and raffinose). Distinctive responses were observed in roots and leaves. By 2DE proteomics a total of 845 spots were observed in leaves; 4.6% changed significantly in response to cold. Twenty-one proteins were identified, many of which were associated with general metabolism or photosynthesis. Transcript levels in leaves were determined by microarray, where dozens of cold associated transcripts were quantitatively characterized, and levels of several potential key contributors (e.g., the dehydrin COR47 and GADb) to cold tolerance were confirmed by qRT-PCR. Cold responses are placed within the existing knowledge base of low temperature stress change in plants, allowing an evaluation of the uniqueness or generality of Fragaria responses in photosynthetic tissues. Overall, the cold response characteristics of ‘Korona’ are consistent with a moderately cold tolerant plant.
Authors
Abdelhameed Elameen Svein Stueland Sonja Klemsdal Ralf Kristensen Rosa Ferreira Fristad Trude Vrålstad Øystein Evensen Ida SkaarAbstract
No abstract has been registered
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Authors
Frederik T. Hansen Donald M. Gardiner Erik Lysøe Patricia Romans Fuertes Bettina Tudzynski Philipp Wiemann Teis Esben Sondergaard Henriette Giese Ditlev E. Brodersen Jens Laurids SørensenAbstract
No abstract has been registered
Authors
Erik Lysøe Rasmus J. N. Frandsen Hege Divon Valeria Terzi Luigi Orrù Antonella Lamontanara Anna-Karin Kolseth Kristian F. Nielsen Ulf ThraneAbstract
No abstract has been registered
Authors
Olav Aaseth Hegnar Gry Alfredsen Vincent Eijsink Carl Gunnar Fossdal Barry Goodell Aniko VarnaiAbstract
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Authors
Richard Strimbeck Paul G. Schaberg Carl Gunnar Fossdal Wolfgang Schröder Trygve Devold KjellsenAbstract
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Authors
Carl Gunnar FossdalAbstract
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Abstract
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Authors
Paal KrokeneAbstract
No abstract has been registered