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NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2008

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Abstract

A 2 x 2 factorial continuous experiment was conducted with 28 Norwegian Red dairy cows in early lactation to compare milk content of phytoestrogens when feeding ad libitum white clover (WCS) or red clover (RCS) grass silages prepared from the second and third cut without and with 10 kg/d supplementation of a standard concentrate. The cows were offered either RCS or WCS for 88 d (period 1) and thereafter a mixed red clover-white clover-grass silage for 48 d (period 2). Total dry matter intake and milk yield were not affected by forage type but increased with concentrate supplementation. Intake of isoflavones was several times greater in RCS than in WCS, whereas intake of lignans was greater in WCS. Concentrate supplementation reduced the intake of most phytoestrogens. Compared with WCS, RCS diets yielded milk with a greater content of flavonoids, whereas milk from WCS diets had greater contents of the mammalian lignans enterodiol and enterolactone. The content of the isoflavan equol was particularly high in RCS diets. There was no apparent carryover effect of clover type on milk phytoestrogen content because there was no difference in content between the silage treatments 3 wk after the cows were transferred to the same silage diet (period 2). Concentrate supplementation reduced the milk contents of the flavonoids equol, biochanin A, and daidzein and increased the content of mammalian lignans. The effects of silage type and concentrate supplementation on milk contents of the individual phytoestrogens were related to the intake of the compound or its precursor, except for the effect of concentrate on mammalian lignans, for which the intake of the known precursors was also reduced. Overall, this study shows that feeding cows with silage containing red clover increases the milk content of flavonoids at both low and high concentrate supplementation levels, and decreases the content of nonflavonoids such as mammalian lignans, when compared with silage containing white clover. The increased content of phytoestrogens in milk may be important when the health benefits of milk are studied.

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Abstract

Red (RC) or white (WC) clover were grown in mixture with grasses, ensiled and offered to dairy cows in early lactation over two successive years (48 cows per year) to compare grassland yield, feed intake, milk production and milk quality. The crops were ensiled in round bales and proportional mixtures of the second and third cut prepared each year were used to ensure that the silage treatments were representative of the crop. In addition to silage type, concentrate supplementation, without and with (10 kg/day), was included as a factor in a 2 X 2 factorial, continuous experiment. Total dry matter (DM) yield, silage chemical composition and total DM intake was hardly affected by silage type. There was no effect of silage type on milk yield and milk constituents either, except for higher milk protein content (P < 0.05) on WC and higher milk fat content of C18:3n-3 (P < 0.001), C18:2n-6 (P < 0.05) fatty acids (FAs) and sum of polyunsaturated FA (P < 0.001) and lower n-6/n-3 FA ratio (P < 0.01) on RC. Concentrate supplementation increased total DM, N and net energy intakes (P < 0.001), milk yield (P < 0.001), milk fat (P < 0.01) and protein (P < 0.001) content, decreased the milk urea content (P < 0.001), and increased the milk fat content of short- and medium-chained FAs (< C16, P < 0.001), C18:0 (P < 0.01) and C18:2n-6 (P < 0.001), decreased the content of C16:0 (P < 0.05), C18:1t11 (P < 0.001) and C 18:3n-3 (P < 0.001), and increased then 6/n-3 FA ratio (P < 0.001). The effect of concentrate supplementation was not affected by silage type, except for milk protein content where the positive effect of supplementation was stronger on WC than on RC diets (P < 0.05). This study illustrates that the white- and red clover-grass mixtures investigated were widely similar with regard to their effects on grassland yield, silage intake and milk production and milk constituents, except for a higher milk fat content of C18:3n-3 and C18:2n-6 and lower n-6/n-3 FA ratio on red clover diets. Our findings also show that N conversion efficiency from feed to milk on pure forage diets is more sensitive to changes in dietary protein intake than silage diets containing cereal based concentrates. (C) 2008 Elsevier B.V. All rights reserved.

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Abstract

Background and aims: White clover (Trifolium repens) is an important component of sustainable livestock systems around the world. Its exploitation for agriculture in the northern, marginal areas, is, however, currently limited by the lack of cultivars that combine persistence and high production potential. The aims are to investigate whether it is feasible to create breeding material of white clover for these areas by combining winter hardiness of northerly populations with good yielding ability of more southerly cultivars. Methods: A total of 166 crosses of 14 different parental combinations between winter-hardy, low-yielding populations of northern origin and high-yielding commercial cultivars of more southerly origin were tested under field conditions in Iceland and Norway and the parental combinations were compared in Norway. Spaced plants were transplanted into a smooth meadow grass (Poa pratensis) sward. Dry matter yield was estimated for 2 years after planting in Norway and morphological characters associated with yielding capacity were measured at both sites. Key results: The results showed that southerly cultivars had larger leaves and higher yielding potential than northern types but suffered more winter damage. Significant variation was found between full-sib families within the different parental combinations for all morphological characteristics measured in all three trials. However, it was difficult to detect any consistens morphological patterns between progeny groups across trial sites. No significant correlations were found between leaflet area and survival. Conclusions: The present study has confirmed that it should be possible to simultaneously select for good winter survival and larger leaves and, hence, higher yielding ability under marginal conditions.