Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2020
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Abstract
Background The Siberian moth (Dendrolimus sibiricus) is a serious pest of conifers in Russia, Northern Kazakhstan, Mongolia and China. The western border of the pest’s distribution in Russia is disputed, but it is present west of the 60th meridian east. The pest has the potential to defoliate a wide range of conifers. Results The pest is not present in Norway or other European countries, except Russia. Natural spread and human mediated transport are potential pathways for the pest. Human mediated pathways considered are: Living trees for planting, coniferous wood in the rough and foliage and branches. There has been no import of living trees from Russia to Norway during the past 30 years, and there is currently no import of coniferous wood commodities containing bark from areas, where D. sibiricus occurs. Conclusions The probability of D. sibiricus entry by natural spread is unlikely, mainly because of the geographical distance and the partial sea barriers between Norway and the infested areas. The probability of entry by human mediated pathways is unlikely due to the very limited volume of the import. Should the pest enter Norway, the probability of establishment and spread is unlikely, due to the suboptimal climatic conditions, and the fact that the two dominant conifers in the country, Norway spruce (Picea abies) and Scots pine (Pinus sylvestris), are intermediate and poor hosts, respectively. The potential damage, should D. sibiricus enter Norway, is considered low. Keywords: Lepidoptera, Invasive species, Geographical distribution, Forest pest
Authors
Tuuli Haikonen Jorunn Børve Timo Kaukoranta Pernilla Gabrielsson Michael Perander Päivi ParikkaAbstract
No abstract has been registered
Authors
Tuuli Haikonen Jorunn Børve Timo Kaukoranta Pernilla Gabrielsson Michael Perander Päivi ParikkaAbstract
No abstract has been registered
Authors
Svein Øivind Solberg Guro Brodal Roland von Bothmer Eivind Meen Flemming Yndgaard Christian Andreasen Åsmund AsdalAbstract
More than 30 years ago, the Nordic Gene Bank established a long-term experiment on seeds stored under permafrost conditions in an abandoned mine corridor in Svalbard, as a tool to monitor storage life under these conditions. The study included seeds from 16 Nordic agricultural and horticultural crops, each represented by two or three cultivars (altogether 38 accessions). All seeds were ultra-dried to 3–5% moisture before being sealed in glass tubes. Germination tests were performed in accordance with the International Seed Testing Association (ISTA) protocols. At the initiation of the experiment, the samples showed good germination with the median value at 92%. The overall picture remained stable over the first twenty to twenty-five years. However, the variation became larger over time and at 30 years, the median value had dropped to 80%. At the lower end, with a high drop in germination, we found rye, wheat, and English ryegrass. At the upper end, we found Kentucky bluegrass and cucumber. The lowest germination was found in samples with the highest initial seed moisture levels. Pre-storage conditions are likely to be of major importance for longevity.
Abstract
During surveys of insect-associated mycobiomes in Norway, Poland, and Russia, isolates with affinity to Graphilbum (Ophiostomatales, Ascomycota) were recovered. In this study, eight known Graphilbum species as well as the newly collected isolates were compared based on morphology and DNA sequence data for four gene regions. The results revealed seven new species, described here as G. acuminatum, G. carpaticum, G. curvidentis, G. furuicola, G. gorcense, G. interstitiale, and G. sexdentatum. In addition to these species, G. crescericum and G. sparsum were commonly found in Norway. All new species were recovered from conifers in association with bark beetles, cerambycid beetles, and weevils and were morphologically similar, predominantly with pesotum-like asexual morphs. Where sexual morphs were present, these were small ascomata with short necks and rodshaped ascospores having hyaline sheaths. The results suggest that Graphilbum species are common members of the Ophiostomatales in conifer ecosystems.
Authors
Beata Strzałka Robert Jankowiak Piotr Bilański Nikita Patel Georg Hausner Riikka Linnakoski Halvor SolheimAbstract
Bark beetles belonging to the genus Dryocoetes (Coleoptera, Curculionidae, Scolytinae) are known vectors of fungi, such as the pathogenic species Grosmannia dryocoetidis involved in alpine fir (Abies lasiocarpa) mortality. Associations between hardwood-infesting Dryocoetes species and fungi in Europe have received very little research attention. Ectosymbiotic fungi residing in Ceratocystiopsis and Leptographium (Ophiostomatales, Sordariomycetes, Ascomycota) were commonly detected in previous surveys of the Dryocoetes alni-associated mycobiome in Poland. The aim of this study was to accurately identify these isolates and to provide descriptions of the new species. The identification was conducted based on morphology and DNA sequence data for six loci (ITS1-5.8S, ITS2-28S, ACT, CAL, TUB2, and TEF1-α). This revealed two new species, described here as Ceratocystiopsis synnemata sp. nov. and Leptographium alneum sp. nov. The host trees for the new species included Alnus incana and Populus tremula. Ceratocystiopsis synnemata can be distinguished from its closely related species, C. pallidobrunnea, based on conidia morphology and conidiophores that aggregate in loosely arranged synnemata. Leptographium alneum is closely related to Grosmannia crassivaginata and differs from this species in having a larger ascomatal neck, and the presence of larger club-shaped cells.
Authors
Carmen Morales-Rodriguez Sten Anslan Marie-Anne Auger-Rozenberg S. Augustin Yuri N. Baranchikov Amani Bellahirech Daiva Burokiene Dovilė Čepukoit Ejup Çota K Davydenko H.T. Doğmuş-Lehtijärvi R. Drenkhan T. Drenkhan Rene Eschen I. Franić M. Glavendekić MB De Groot Magdalena Kacprzyk Marc Kenis Natalia Kirichenko I. Matsiakh DL Musolin Justyna A. Nowakowska Richard O’Hanlon S. Prospero A Roques A Santini Venche Talgø Leho Tedersoo A Uimari A. Vannini J Witzell S Woodward Antonios Zambounis Michelle ClearyAbstract
No abstract has been registered
Authors
Ranjana Pathak Åshild Ergon Arne Stensvand Hans Ragnar Gislerød Knut Asbjørn Solhaug Lance Cadle-Davidson Aruppillai SuthaparanAbstract
Powdery mildews can be controlled by brief exposure to ultraviolet (UV) radiation with devastating effect on their developmental stages including conidia germination. The treatment effect can be impaired by subsequent exposure to UV-A/blue light. UV-A/blue light-activated photolyase may be responsible for this and therefore we tested the function of three cryptochrome/photolyase family (CPF)-like genes (OINE01015670_T110144, OINE01000912_T103440, and OINE01005061_T102555) identified in the obligate biotrophic fungus Pseudoidium neolycopersici, the cause of tomato powdery mildew. A photolyase-deficient mutant of Escherichia coli transformed with coding sequence of OINE01000912_T103440 and exposed to brief (UV)-C treatment (peak emission at 254 nm) showed photoreactivation and cell survival when exposed to subsequent blue light, indicating complementation of photolyase activity. In contrast, the same photolyase-deficient E. coli transformed with the coding sequences of other two CPF-like genes did not survive this treatment, even though their expression were confirmed at protein level. This confirmed that OINE01000912_T103440 is a gene encoding photolyase, here named PnPHR1, with functionality similar to the native photolyase in E. coli, and classified as a class I cyclobutane pyrimidine dimer (CPD) photolyase. Modeling of the 634-amino acid sequence of PnPHR1 suggested that it is capable of binding flavin adenine dinucleotide (FAD) and methenyltetrahydrofolate (MTHF). However, spectroscopic data of the protein produced in an E. coli expression system could only reveal the presence of a reduced form of FAD, i.e., FADH– as an intrinsic chromophore. Within the tested wavelength range of 365–525 nm, the survival of photolyase-deficient mutant E. coli transformed with PnPHR1 showed a broad action spectrum from 365 to 454 nm. This was very similar to the previously characterized action spectrum for survival of P. neolycopersici conidia that had been treated with UV-C. Quantitative RT-PCR revealed that the expression of PnPHR1 in P. neolycopersici conidia was induced by UV-C, and peak expression occurred 4 h after brief UV-C treatment. The expression of PnPHR1 was repressed when incubated in red light after the UV-C treatment, but not when incubated in UV-A/blue light. The results may explain why the disease-reducing effect of short wavelength UV is impaired by exposure to UV-A and blue light.