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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2021

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Abstract

The biosynthesis of anthocyanins has been shown to be influenced by light quality. However, the molecular mechanisms underlying the light-mediated regulation of fruit anthocyanin biosynthesis are not well understood. In this study, we analysed the effects of supplemental red and blue light on the anthocyanin biosynthesis in non-climacteric bilberry (Vaccinium myrtillus L.). After 6 days of continuous irradiation during ripening, both red and blue light elevated concentration of anthocyanins, up to 12- and 4-folds, respectively, compared to the control. Transcriptomic analysis of ripening berries showed that both light treatments up-regulated all the major anthocyanin structural genes, the key regulatory MYB transcription factors and abscisic acid (ABA) biosynthetic genes. However, higher induction of specific genes of anthocyanin and delphinidin biosynthesis alongside ABA signal perception and metabolism were found in red light. The difference in red and blue light signalling was found in 9-cis-epoxycarotenoid dioxygenase (NCED), ABA receptor pyrabactin resistance-like (PYL) and catabolic ABA-8'hydroxylase gene expression. Red light also up-regulated expression of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) domain transporters, which may indicate involvement of these proteins in vesicular trafficking of anthocyanins during fruit ripening. Our results suggest differential signal transduction and transport mechanisms between red and blue light in ABA-regulated anthocyanin and delphinidin biosynthesis during bilberry fruit ripening.

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Abstract

Compact and healthy seedlings of Brassica increase the crop production and improve vegetable quality. Different climatic conditions and extensive light can cause young plants to become elongated and spindly. We investigated the effects of end-of-day (EOD) treatment on plant height (cm) of the seedlings of three cultivars of each of the following species: cauliflower (‘Delfino’, ‘Freedom’ and ‘Momentum’), broccoli (‘Achilles’, ‘Ironman’ and ‘Steel’), Brussels sprouts (‘Gladius’, ‘Brigitte’ and ‘Maximus’) and swede (‘Vigod’, ‘Vige’ and ‘Neve’) using red light (RL) with wavelength of 600 nm and intensity of 10 μmol m2 s‑1 as a supplement to natural light. All seedlings were planted in an experimental field. Yield of the mentioned species and vitamin C content were measured after harvest. Night EOD treatment resulted in 7% shorter and more compact plants in broccoli (p=0.04). There were yield differences (31-44%) among cultivars within the cauliflower (p=0.001), broccoli (p=0.01) and Brussels sprouts (p=0.001). There were significant differences in vitamin C content among cultivars in all four Brassica species (p<0.01). This study provides new information about the effect of night break treatment with red light on seedling length in broccoli, yield differences in cultivars of cauliflower, broccoli and Brussels sprouts and on vitamin C content in different cultivars of four studied Brassica species.