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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2007

Abstract

The aim of this study was to use Energy-Dispersive X-ray Spectroscopy (EDS) to localize chitosan in the cell wall of chitosan impregnated Scots pine. It is of both general and specific interest to investigate the concentration of chitosan in the wood matrix to gain further knowledge and understanding of chitosan as a wood protective system.After deacetylation, chitosan was re-acetylated with chloroacetic anhydride to achieve a covalent bonding of chloride to the chitosan polymer. Chloride labeled chitosan was measured by EDS using a Scanning Electron Microscope (SEM) and described in apparent concentration of chloride. Analysis for free chloride anions was tested by dialysis and ion chromatography.There was a significant correlation between the molecular weight of chitosan and the apparent concentration of covalent bonded chloride to the chitosan polymer. High molecular weight chitosan showed a better interaction with the cell wall structure than low molecular chitosan.

Abstract

Histological properties of the graft union between a rootstock and scion may provide a mechanistic explanation why dwarfing rootstocks are able to reduce the growth of the tree. In order to investigate the relationship between growth and the amount of functional xylem tissue of potted sweet cherry trees, an experiment was conducted during 2002-2003. One year old field budded and bench grafted (from greenhouse) sweet cherry trees of the cultivars Van, Ulster and Lapins in all combinations of the three rootstocks Prunus avium seedling, Colt and Gisela 5 were grown in pots for two growing seasons. After shoot extension had terminated when trees were in full leaf the second year, the total growth of the different parts of the trees and the functional area using safranin staining were measured. The total mass production (tree dry weight, the length of two year old wood and number of leaves) was significantly larger in the budded trees. Similarly the trunk cross sectional area was significantly larger 10 cm above and in the middle of the graft union, but not 10 cm below it. The Colt trees were the most vigorous followed by the seedling and Gisela 5. Minor differences between the different cultivars were registered. Xylem staining with aqueous safranin combined with quantitative image analysis showed that the rootstock stem had a higher proportion of stained tissue than the scion stem. Sections taken in the middle of the graft tissue and 10 cm above showed that the proportion of stained tissue declined proportionally with the distance from the roots. The total area of stained stem xylem was larger for the two vigorous rootstocks compared to Gisela 5. Small differences were observed between cultivars and propagation methods.