Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2012
Authors
Muath Alsheikh-Yousef Gage Koehler Robert Wilson Jens Rohloff Anita Sønsteby Jahn Davik Stephen K, RandallAbstract
A crucial consideration for strawberry producers in Norway and other northern countries is winter freezing damage. A long-term goal of the Norwegian strawberry breeding is to increase winter hardiness and to improve fruit quality. Due to the complexity involved in regulating and enhancing freezing tolerance, the progress in the improvement of cultivars using traditional screening methods have had limited success. Thus, the development of molecular markers for freezing hardiness would facilitate the selection work for this trait. In this effort, we have developed and adopted state-of-art molecular tools to investigate cold response in strawberry plants during the acclimation phase resulting in the identification of a large number of genes, proteins, and distinct metabolites that correspond to cold/freezing tolerance in strawberry. To identify proteins responsible for freezing tolerance in strawberry we have examined alterations in protein levels in strawberry varieties that differ in cold tolerance using either 2-DE gel analysis followed by LC-MS/MS analysis or a shotgun MS/MS approach. Proteomic analysis suggested 30 potential biomarkers that showed significant changes in the cultivated strawberry in response to cold. In addition, GC-MS-based metabolite profiling revealed the up-regulation of carbohydrates, polyols, amino acids, TCA intermediates, and other distinct secondary metabolites after cold treatment. Transcriptional analysis of the cold acclimated samples also confirmed the regulation upon cold-treatment with varietal differences in strawberry. Moreover, several F2-populations from the model F. vesca parents diverging in cold tolerance have been developed in order to facilitate mapping of QTLs by performing GBS analyses. The knowledge attained from these endeavors is expected to expedite breeding of strawberries to achieve freezing tolerant lines and provide an integrative understanding of the molecular pathways that underlie this characteristic. * Rohloff et al. (2012) Metabolite profiling reveals novel multi-level cold responses in the model Fragaria vesca. Phytochemistry 79:99-109. * Koehler et al. (2012) Proteomic study of low temperature responses in strawberry cultivars (Fragaria x ananassa) that differ in cold tolerance. Plant Physiology 159:1787–1805 * Davik et al., (2012) Low temperature tolerance in diploid strawberry species (Fragaria ssp.) and its correlation to alcohol dehydrogenase levels, dehydrin levels, and central metabolism constituents. Planta (in press; DOI: 10.1007/s00425-012-1771-2).
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Authors
Anette SundbyeAbstract
Pest and disease management in organic greenhouse production in Norway Anette Sundbye1, Nina Svae Johansen2, Arne Stensvand3 1, 2, 3 Norwegian Institute for Agricultural and Environmental Research (Bioforsk) - Plant Health and Plant Protection, Email1: anette.sundbye@bioforsk.no Development of organic farming and marketing of organic products is a political priority in Norway, and the major goal is that 15% of the food production and consumption in year 2020 should be organic. This also relates to vegetables in greenhouses. The demand for organic vegetables is increasing and the consumers are increasingly more conscious of how their food is produced. Norwegian growers who choose to convert to organic cultivation have major challenges when it comes to marketing and keeping a stable production. However, guidance by the Norwegian agricultural extension service on organic production of cucumber has been successful (project “Organic cultivation of greenhouse vegetables and herbs” 2010-2012). Also growers of tomatoes, lettuce and herbs are in good progress in converting to organic production. According to current Norwegian regulations, the organic greenhouse production should mainly be based on natural light. The need for and use of artificial light should be documented and can only be used in certain periods of the cultivation time. The main goal of the project “Environmentally friendly development of Norwegian greenhouse industry (2009-2012)” is to reduce energy consumption in plant production. This is practiced by maximizing the utilization of natural radiation and manipulating the light with different greenhouse covering, shading materials and LED based lamps with specific wavelength spectrum. The effect of light quality on powdery mildew and pests is also studied. Experiments have shown that illumination with red or UV-B light some minutes a day can reduce powdery mildew significantly. Blue sticky traps equipped with low intensity LEDs have the potential to increase thrips catches on sticky traps, and lamps with repellent wavelengths may be used to confuse whiteflies in their host finding. Only a limited number of biological control agents (BCA) are currently registered in Norway. On the international market, a wide range of commercially products of BCA is available. Access to these products would benefit the Norwegian organic production. In order to increase the availability and use of BCA in Norway, two projects have been funded by the Ministry of Food and Agriculture. In the new project “Increasing the use of biological control agents of plant pests” (2012) bottlenecks will be identified and solution will be proposed to promote the registration and increase the use of BCA in Norway. The other project “Extension in greenhouse biological control” (2006-2012) has increased the implementation of biological control and IPM in Norwegian commercial greenhouses.
Authors
Zehra Sapci Maria Magdalena Estevez Roar Linjordet John MorkenAbstract
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Authors
Ricardo HolgadoAbstract
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Ricardo HolgadoAbstract
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Ricardo HolgadoAbstract
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Lise SkrettebergAbstract
No abstract has been registered