Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2003
Forfattere
Halvor Solheim Kåre Olav VennSammendrag
No abstract has been registered
Forfattere
Stephen Woodward Jan Stenlid Marco Michelozzi Halvor Solheim B. Karlsson Panaghiotis TsopelasSammendrag
No abstract has been registered
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Halvor SolheimSammendrag
No abstract has been registered
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M. Persson J. Lönnquist Bo Långström Halvor Solheim M. Kyto Anna-Karin Borg-KarlsonSammendrag
No abstract has been registered
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Halvor SolheimSammendrag
No abstract has been registered
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Øystein Johnsen Carl Gunnar Fossdal Rüdiger Baumann Jørgen A. Mølmann Ola Gram Dæhlen David ClaphamSammendrag
No abstract has been registered
Forfattere
Øystein Johnsen Carl Gunnar Fossdal Rüdiger Baumann Jørgen A. Mølmann Ola Gram Dæhlen David Clapham Tore SkrøppaSammendrag
No abstract has been registered
Sammendrag
A multiplex real-time PCR assay was developed to monitor the dynamics of the Picea abies-Heterobasidion annosum pathosystem. Tissue cultures and 32-year-old trees with low or high resistance to this pathogen were used as the host material. Probes and primers were based on a laccase gene for the pathogen and a polyubiquitin gene for the host.The real-time PCR procedure was compared to an ergosterol-based quantification method in a tissue culture experiment, and there was a strong correlation product moment correlation coefficient, 0.908) between the data sets. The multiplex real-time PCR procedure had higher resolution and sensitivity during the early stages of colonization and also could be used to monitor the host.In the tissue culture experiment, host DNA was degraded more rapidly in the clone with low resistance than in the clone with high resistance. In the field experiment, the lesions elicited were not strictly proportional to the area colonized by the pathogen.Fungal colonization was more restricted and localized in the lesion in the clone with high resistance, hereas in the clone with low resistance, the fungus could be detected until the visible end of the lesion. Thus, the real-time PCR assay gives better resolution than does the traditionally used lesion length measurement when screening host clones for resistance.
2002
Sammendrag
In 2001, a zone site C, was established as a circular areas with 50 km radius and centred in Greåker close to Sarpsborg in South-eastern Norway. Zone site C is complementary to the similar zone sites A and B established in 2000. From June 2001 to December 2002, 601 wood samples were collected from 66 forest blocks, all situated within the 3 existing zone sites A, B and C. The sampling was carried out in the provinces Akershus, Buskerud, Vestfold and Østfold, but was concentrated to the South-eastern region, where 399 samples were taken from 45 forest blocks in the province of Østfold. Samples from Scots pine (Pinus sylvestris) formed 96% of the collected material, while Norway spruce (Picea abies) was a minor fraction. Lying trees and various kinds of detached wood formed the main part of the objects sampled. The frequency of objects showing signs of attack by wood boring insects was 44%. Some samples were also taken in Porsgrunn in the province of Telemark from a consignment of spruce imported from Russia. Nematodes were often more common in samples from objects with signs of insect activity. In four samples, all from lying pine trees, nematodes belonging to the genus Bursaphelenchus were detected. Bursaphelenchus mucronatus was recorded for the second time in Norway, and occurred in a lying pine tree attacked by Monochamus at Ombudstvedt in the municipality of Våler in the province of Østfold. The pine wood nematode Bursaphelenchus xylophilus was not detected, nor in forests or in 5 samples from spruce imported from Russia
Sammendrag
No abstract has been registered