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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2022

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Abstract

Plant selection for rain gardens along streets and roads in cold climates can be complicated, as the plants are subjected to combined stresses including periodic inundation, de-icing salts, road dust, splashes of water from the road, freezing and thawing of soil, and periods with ice cover during the winter. The purpose of this study was to identify species suited to grow in these conditions and determine their optimal placement within roadside rain gardens. Thirty-one herbaceous perennial species and cultivars were planted in real-scale rain gardens in a street in Drammen (Norway) with supplemental irrigation, and their progress was recorded during the following three growing seasons. The study highlights considerable differences between species’ adaptation to roadside rain gardens in cold climates, especially closest to the road. Some candidate species/cultivars had a high survival rate in all rain garden positions and were developed well. These were: Amsonia tabernaemontana, Baptisia australis, Calamagrostis × acutiflora ‘Overdam’, Hemerocallis ‘Camden Gold Dollar’, Hemerocallis ‘Sovereign’, Hemerocallis lilioasphodelus, Hosta ‘Sum & Substance’, Iris pseudacorus and Liatris spicata ‘Floristan Weiss’. Other species/cultivars appeared to adapt only to certain parts of the rain garden or had medium tolerance. These were: Calamagrostis brachytricha, Carex muskingumensis, Eurybia × herveyi ‘Twilight’, Hakonechloa macra, Hosta ‘Francee’, Hosta ‘Striptease’, Liatris spicata ‘Alba’, Lythrum salicaria ‘Ziegeunerblut’, Molinia caerulea ‘Moorhexe’, Molinia caerulea ‘Overdam’, and Sesleria autumnalis. Species/cultivars that showed high mortality and poor development at all rain garden positions should be avoided in roadside cold climate rain gardens. These include Amsonia orientalis, Aster incisus ‘Madiva’, Astilbe chinensis var. tacquettii ‘Purpurlanze’, Chelone obliqua, Dryopteris filix-mas, Eurybia divaricata, Geranium ‘Rozanne’, Helenium ‘Pumilum Magnificum’, Luzula sylvatica, Polygonatum multiflorum and Veronicastrum virginicum ‘Apollo’. The study also found considerable differences between cultivars within the same species, especially for Hosta cvv. and Liatris spicata. Further investigations are needed to identify the cultivars with the best adaption to roadside rain gardens in cold climates.

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Abstract

The Pasvik River experiences chemical, physical, and biological stressors due to the direct discharges of domestic sewage from settlements located within the catchment and runoff from smelter and mine wastes. Sediments, as a natural repository of organic matter and associated contaminants, are of global concern for the possible release of pollutants in the water column, with detrimental effects on aquatic organisms. The present study was aimed at characterizing the riverine benthic microbial community and evaluating its ecological role in relation to the contamination level. Sediments were sampled along the river during two contrasting environmental periods (i.e., beginning and ongoing phases of ice melting). Microbial enzymatic activities, cell abundance, and morphological traits were evaluated, along with the phylogenetic community composition. Amplified 16S rRNA genes from bacteria were sequenced using a next-generation approach. Sediments were also analyzed for a variety of chemical features, namely particulate material characteristics and concentration of polychlorobiphenyls, polycyclic aromatic hydrocarbons, and pesticides. Riverine and brackish sites did not affect the microbial community in terms of main phylogenetic diversity (at phylum level), morphometry, enzymatic activities, and abundance. Instead, bacterial diversity in the river sediments appeared to be influenced by the micro-niche conditions, with differences in the relative abundance of selected taxa. In particular, our results highlighted the occurrence of bacterial taxa directly involved in the C, Fe, and N cycles, as well as in the degradation of organic pollutants and toxic compounds.

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Abstract

Production of biochar from corn cob and corn stalk has gained great interest for efficient waste management with benefits of improving soil properties, increasing crop productivity, and contributing to carbon sequestration. This study investigated slow pyrolysis of corn cob and corn stalk at 600 °C to characterize yields and properties of products, with focus on solid biochar. Spruce wood, a rather well studied woody biomass, was also included for comparison purposes. It was observed that yields of biochar and condensates from corn cob, corn stalk, and spruce wood were comparable. However, gas release profiles and yields from the three biomasses were quite different, which is mainly related to the different chemical compositions (i.e., hemicellulose, cellulose, lignin, and inorganic species) of the studied raw feedstocks. The produced biochars were analyzed for proximate analysis, CHNS-elemental analysis, specific surface area and specific pore volume for pores in the nm-range, inorganic composition, solid functional groups, and aromaticity. The corn cob and corn stalk biochar presented significantly higher concentration of inorganic elements, especially P and K, favoring soil application. The SEM analysis results showed that the spruce wood biochar has different microstructure than corn cob and corn stalk biochars. Condensates and light gases, as by-products from biochar production, contained over 50% of the energy and 40% of the total carbon of the initial biomass. Utilization of the condensates and light gases as valuable resources is therefore critical for improving environmental and energy benefits of the biochar production process.

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Abstract

Despite the availability of improved antiviral therapies, infection with Hepatitis B virus (HBV) remains a3 significant health issue, as a curable treatment is yet to be discovered. Current HBV vaccines relaying on the efficient expression of the small (S) envelope protein in yeast and the implementation of mass vaccination programs have clearly contributed to containment of the disease. However, the lack of an efficient immune response in up to 10% of vaccinated adults, the controversies regarding the seroprotection persistence in vaccine responders and the emergence of vaccine escape virus mutations urge for the development of better HBV immunogens. Due to the critical role played by the preS1 domain of the large (L) envelope protein in HBV infection and its ability to trigger virus neutralizing antibodies, including this protein in novel vaccine formulations has been considered a promising strategy to overcome the limitations of S only-based vaccines. In this work we aimed to combine relevant L and S epitopes in chimeric antigens, by inserting preS1 sequences within the external antigenic loop of S, followed by production in mammalian cells and detailed analysis of their antigenic and immunogenic properties. Of the newly designed antigens, the S/preS116–42 protein assembled in subviral particles (SVP) showed the highest expression and secretion levels, therefore, it was selected for further studies in vivo. Analysis of the immune response induced in mice vaccinated with S/preS116–42- and S-SVPs, respectively, demonstrated enhanced immunogenicity of the former and its ability to activate both humoral and cellular immune responses. This combined activation resulted in production of neutralizing antibodies against both wild-type and vaccine-escape HBV variants. Our results validate the design of chimeric HBV antigens and promote the novel S/preS1 protein as a potential vaccine candidate for administration in poor-responders to current HBV vaccines.