Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2020
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Tuuli Haikonen Jorunn Børve Timo Kaukoranta Pernilla Gabrielsson Michael Perander Päivi ParikkaAbstract
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Tuuli Haikonen Jorunn Børve Timo Kaukoranta Pernilla Gabrielsson Michael Perander Päivi ParikkaAbstract
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Authors
Svein Øivind Solberg Guro Brodal Roland von Bothmer Eivind Meen Flemming Yndgaard Christian Andreasen Åsmund AsdalAbstract
More than 30 years ago, the Nordic Gene Bank established a long-term experiment on seeds stored under permafrost conditions in an abandoned mine corridor in Svalbard, as a tool to monitor storage life under these conditions. The study included seeds from 16 Nordic agricultural and horticultural crops, each represented by two or three cultivars (altogether 38 accessions). All seeds were ultra-dried to 3–5% moisture before being sealed in glass tubes. Germination tests were performed in accordance with the International Seed Testing Association (ISTA) protocols. At the initiation of the experiment, the samples showed good germination with the median value at 92%. The overall picture remained stable over the first twenty to twenty-five years. However, the variation became larger over time and at 30 years, the median value had dropped to 80%. At the lower end, with a high drop in germination, we found rye, wheat, and English ryegrass. At the upper end, we found Kentucky bluegrass and cucumber. The lowest germination was found in samples with the highest initial seed moisture levels. Pre-storage conditions are likely to be of major importance for longevity.
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Carmen Morales-Rodriguez Sten Anslan Marie-Anne Auger-Rozenberg S. Augustin Yuri N. Baranchikov Amani Bellahirech Daiva Burokiene Dovilė Čepukoit Ejup Çota K Davydenko H.T. Doğmuş-Lehtijärvi R. Drenkhan T. Drenkhan Rene Eschen I. Franić M. Glavendekić MB De Groot Magdalena Kacprzyk Marc Kenis Natalia Kirichenko I. Matsiakh DL Musolin Justyna A. Nowakowska Richard O’Hanlon S. Prospero A Roques A Santini Venche Talgø Leho Tedersoo A Uimari A. Vannini J Witzell S Woodward Antonios Zambounis Michelle ClearyAbstract
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Authors
Ranjana Pathak Åshild Ergon Arne Stensvand Hans Ragnar Gislerød Knut Asbjørn Solhaug Lance Cadle-Davidson Aruppillai SuthaparanAbstract
Powdery mildews can be controlled by brief exposure to ultraviolet (UV) radiation with devastating effect on their developmental stages including conidia germination. The treatment effect can be impaired by subsequent exposure to UV-A/blue light. UV-A/blue light-activated photolyase may be responsible for this and therefore we tested the function of three cryptochrome/photolyase family (CPF)-like genes (OINE01015670_T110144, OINE01000912_T103440, and OINE01005061_T102555) identified in the obligate biotrophic fungus Pseudoidium neolycopersici, the cause of tomato powdery mildew. A photolyase-deficient mutant of Escherichia coli transformed with coding sequence of OINE01000912_T103440 and exposed to brief (UV)-C treatment (peak emission at 254 nm) showed photoreactivation and cell survival when exposed to subsequent blue light, indicating complementation of photolyase activity. In contrast, the same photolyase-deficient E. coli transformed with the coding sequences of other two CPF-like genes did not survive this treatment, even though their expression were confirmed at protein level. This confirmed that OINE01000912_T103440 is a gene encoding photolyase, here named PnPHR1, with functionality similar to the native photolyase in E. coli, and classified as a class I cyclobutane pyrimidine dimer (CPD) photolyase. Modeling of the 634-amino acid sequence of PnPHR1 suggested that it is capable of binding flavin adenine dinucleotide (FAD) and methenyltetrahydrofolate (MTHF). However, spectroscopic data of the protein produced in an E. coli expression system could only reveal the presence of a reduced form of FAD, i.e., FADH– as an intrinsic chromophore. Within the tested wavelength range of 365–525 nm, the survival of photolyase-deficient mutant E. coli transformed with PnPHR1 showed a broad action spectrum from 365 to 454 nm. This was very similar to the previously characterized action spectrum for survival of P. neolycopersici conidia that had been treated with UV-C. Quantitative RT-PCR revealed that the expression of PnPHR1 in P. neolycopersici conidia was induced by UV-C, and peak expression occurred 4 h after brief UV-C treatment. The expression of PnPHR1 was repressed when incubated in red light after the UV-C treatment, but not when incubated in UV-A/blue light. The results may explain why the disease-reducing effect of short wavelength UV is impaired by exposure to UV-A and blue light.
Authors
Ranjana Pathak Åshild Ergon Arne Stensvand Hans Ragnar Gislerød Knut Asbjørn Solhaug Lance Cadle-Davidson Aruppillai SuthaparanAbstract
No abstract has been registered
Authors
Arne StensvandAbstract
No abstract has been registered