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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2022

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Abstract

Litter comprises a major nutrient source when decomposed via soil microbes and functions as subtract that limits gas exchange between soil and atmosphere, thereby restricting methane (CH4) uptake in soils. However, the impact and inherent mechanism of litter and its decomposition on CH4 uptake in soils remains unknown in forest. Therefore, to declare the mechanisms of litter input and decomposition effect on the soil CH4 flux in forest, this study performed a litter-removal experiment in a tropical rainforest, and investigated the effects of litter input and decomposition on the CH4 flux among forest ecosystems through a literature review. Cumulative annual CH4 flux was −3.30 kg CH4-C ha−1 y−1. The litter layer decreased annual accumulated CH4 uptake by 8% which greater in the rainy season than the dry season in the tropical rainforest. Litter decomposition and the input of carbon and nitrogen in litter biomass reduced CH4 uptake significantly and the difference in CH4 flux between treatment with litter and without litter was negatively associated with N derived from litter input. Based on the literature review about litter effect on soil CH4 around world forests, the effect of litter dynamics on CH4 uptake was regulated by litter-derived nitrogen input and the amount soil inorganic nitrogen content. Our results suggest that nitrogen input via litter decomposition, which increased with temperature, caused a decline in CH4 uptake by forest soils, which could weaken the contribution of the forest in mitigating global warming.

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Abstract

The rapid conversion of tropical rainforests into monoculture plantations of rubber (Hevea brasiliensis) in Southeast Asia (SEA) necessitates understanding of rubber tree physiology under local climatic conditions. Frequent fog immersion in the montane regions of SEA may affect the water and carbon budgets of the rubber trees and the plantation ecosystems. We studied the effect of fog on various plant physiological parameters in a mature rubber plantation in southwest China over 3 years. During the study period, an average of 141 fog events occurred every year, and the majority occurred during the dry season, when the temperature was relatively low. In addition to the low temperature, fog events were also associated with low vapor pressure deficit, atmospheric water potential, relative humidity and frequent wet-canopy conditions. We divided the dry season into cool dry (November-February) and hot dry (March-April) seasons and classified days into foggy (FG) and non-foggy (non-FG) days. During the FG days of the cool dry season, the physiological activities of the rubber trees were suppressed where carbon assimilation and evapotranspiration showed reductions of 4% and 15%, respectively, compared to the cool dry non-FG days. Importantly, the unequal declines in carbon assimilation and evapotranspiration led to enhanced crop water productivity (WPc) on cool dry FG days but insignificant WPc values were found between FG and non-FG days of the hot dry season. Our results suggest that, by regulating plant physiology, fog events during the cool dry season significantly reduce water demand and alleviate water stress for the trees through improved WPc.

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Abstract

Peatlands have acted as net CO2 sinks over millennia, exerting a global climate cooling effect. Rapid warming at northern latitudes, where peatlands are abundant, can disturb their CO2 sink function. Here we show that sensitivity of peatland net CO2 exchange to warming changes in sign and magnitude across seasons, resulting in complex net CO2 sink responses. We use multiannual net CO2 exchange observations from 20 northern peatlands to show that warmer early summers are linked to increased net CO2 uptake, while warmer late summers lead to decreased net CO2 uptake. Thus, net CO2 sinks of peatlands in regions experiencing early summer warming, such as central Siberia, are more likely to persist under warmer climate conditions than are those in other regions. Our results will be useful to improve the design of future warming experiments and to better interpret large-scale trends in peatland net CO2 uptake over the coming few decades.

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Abstract

Production of biochar from corn cob and corn stalk has gained great interest for efficient waste management with benefits of improving soil properties, increasing crop productivity, and contributing to carbon sequestration. This study investigated slow pyrolysis of corn cob and corn stalk at 600 °C to characterize yields and properties of products, with focus on solid biochar. Spruce wood, a rather well studied woody biomass, was also included for comparison purposes. It was observed that yields of biochar and condensates from corn cob, corn stalk, and spruce wood were comparable. However, gas release profiles and yields from the three biomasses were quite different, which is mainly related to the different chemical compositions (i.e., hemicellulose, cellulose, lignin, and inorganic species) of the studied raw feedstocks. The produced biochars were analyzed for proximate analysis, CHNS-elemental analysis, specific surface area and specific pore volume for pores in the nm-range, inorganic composition, solid functional groups, and aromaticity. The corn cob and corn stalk biochar presented significantly higher concentration of inorganic elements, especially P and K, favoring soil application. The SEM analysis results showed that the spruce wood biochar has different microstructure than corn cob and corn stalk biochars. Condensates and light gases, as by-products from biochar production, contained over 50% of the energy and 40% of the total carbon of the initial biomass. Utilization of the condensates and light gases as valuable resources is therefore critical for improving environmental and energy benefits of the biochar production process.

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Abstract

Despite the availability of improved antiviral therapies, infection with Hepatitis B virus (HBV) remains a3 significant health issue, as a curable treatment is yet to be discovered. Current HBV vaccines relaying on the efficient expression of the small (S) envelope protein in yeast and the implementation of mass vaccination programs have clearly contributed to containment of the disease. However, the lack of an efficient immune response in up to 10% of vaccinated adults, the controversies regarding the seroprotection persistence in vaccine responders and the emergence of vaccine escape virus mutations urge for the development of better HBV immunogens. Due to the critical role played by the preS1 domain of the large (L) envelope protein in HBV infection and its ability to trigger virus neutralizing antibodies, including this protein in novel vaccine formulations has been considered a promising strategy to overcome the limitations of S only-based vaccines. In this work we aimed to combine relevant L and S epitopes in chimeric antigens, by inserting preS1 sequences within the external antigenic loop of S, followed by production in mammalian cells and detailed analysis of their antigenic and immunogenic properties. Of the newly designed antigens, the S/preS116–42 protein assembled in subviral particles (SVP) showed the highest expression and secretion levels, therefore, it was selected for further studies in vivo. Analysis of the immune response induced in mice vaccinated with S/preS116–42- and S-SVPs, respectively, demonstrated enhanced immunogenicity of the former and its ability to activate both humoral and cellular immune responses. This combined activation resulted in production of neutralizing antibodies against both wild-type and vaccine-escape HBV variants. Our results validate the design of chimeric HBV antigens and promote the novel S/preS1 protein as a potential vaccine candidate for administration in poor-responders to current HBV vaccines.