NIBIO's Scientific Publications
This list contains articles, books and chapters that are published in authorised publication channels in The Norwegian Register for Scientific Journals, Series and Publishers. The register shows which scientific publications are recognized in the weighted funding model. The list is sorted by latest registered publication.
2018 (305)
Authors
Habtamu AlemAbstract
In this article, we estimate the progress of Total Factor Productivity (TFP) in the Norwegian grain production sector. Previous studies conducted in TFP estimation can be criticized for estimated production function relied on the assumption that the underlying technology is the same for all regions and firms face similar environmental conditions. In reality, agricultural firms in different regions resource endowment, adoption of new technology, and innovation might be different because of farmers face different production opportunities. For this study, we classified the country into two main grain producing regions with district level of development, and hence production technologies. We used farm level balanced panel data for 19 years (1996-2014) with 1463 observations from farms specialized in grain production. We applied the ‘true' fixed effect stochastic frontier model to estimate region level efficiency and source of productivity changes. The result of the analysis shows that there has been a productivity improvement in the sector, and technical change has had the main source of productivity change.
Authors
Sekhar Udaya NagothuAbstract
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Authors
Ketil HaarstadAbstract
Three treatment systems for wastewater from two landfills, one active and one closed, and an industrial location including a quarry have been monitored continuously for over a decade. The wastewater from the active landfill is infiltrated through an extensive unsaturated zone into groundwater and subsequently into a large river system. The wastewater from the closed landfill is treated in a constructed wetland (CW) and the industrial low-grade wastewater in filter dams. The treatment systems operate well with the specific wastewaters, high-concentration leachate from waste in infiltration systems, low-concentration leachate in constructed wetlands, and wastewater from inert waste in filter dams. The landfilling of organic waste was restricted to low limit values for more than a decade ago, but it is hard to see any changes in leachate due to changes in waste landfilling regulations. The heavy carbon stable isotope 13C is useful in tracing landfill leachate and to evaluate dilution into other water bodies. The adding of P to the aeration pond treating low-concentration leachate did not help in the removal of N; on the contrary, the concentration of ammonia was sharply decreased when the adding of P was discontinued.
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Authors
Ngo Tien Dung Sekhar Udaya Nagothu Alma Linda Morales-Abubakar Jan Willem Ketelaar Mehreteab TesfaiAbstract
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Authors
Isaiah Nyagumbo Mehreteab Tesfai Sekhar Udaya Nagothu Peter Setimela James K. Karanja Munyaradzi Mutenje Connie MademboAbstract
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Authors
Fernanda de Cássia Neves Esteca Luis Rodolfo Rodrigues Gilberto José de Moraes Italo, Júnior Delalibera Ingeborg KlingenAbstract
Mulching of soil beds of strawberry fields is usually done with polyethylene film in southern Minas Gerais state, Brazil. This material is relatively expensive and difficult to discard after use. In some countries, mulching is done with the use of organic material that could have an advantage over the use of plastic for its easier degradation after use, and for favoring edaphic beneficial organisms. Predatory mites (especially Gamasina, Mesostigmata) may be abundant in the soil and could conceivably move to the soil surface and onto the short-growing strawberry plants at night, helping in the control or pest arthropods. The two-spotted spider mite, Tetranychus urticae Koch, is considered an important strawberry pest in that region, where the fungus Neozygites floridana (Weiser and Muma) has been found to infect it. Different mulching types could affect the incidence of this pathogen. Dehydrated coffee husk and pulp (DCHP) is a byproduct readily available in southern Minas Gerais, where could be used as organic mulching in strawberry beds. The temporary contact of that material with the soil of a patch of natural vegetation could facilitate its colonization by edaphic predatory mites helpful in the control of strawberry pests. The objective of this work was to study the effect of mulching type on the population dynamics of the two-spotted spider mite, associate mites and N. floridana, in a greenhouse and in the field. The use of DCHP increased the number of edaphic Gamasina on strawberry plants—Proctolaelaps pygmaeus (Müller) (Melicharidae) and Blattisocius dentriticus (Berlese) (Blattisociidae) were observed on strawberry leaflets, mainly in nocturnal samplings, indicating their possible daily migration from soil to plants. Lower levels of two-spotted spider mite occurred on plants from pots or soil beds mulched with DCHP instead of polyethylene film, possibly because of the slightly higher levels of mites of the family Phytoseiidae and infection by N. floridana. Adding DCHP onto the floor of natural vegetation did not result in higher diversity or levels of gamasine mites on DCHP. Complementary studies should be conducted to find ways to increase diversity and density of those organisms in strawberry beds, in an attempt to improve biological control of strawberry pests. The decision to use DCHP for mulching should also take into account other factors such as strawberry yield, costs and efficiency of weed management, to be evaluated in subsequent studies.
Authors
Daniel R. Hirmas Daniel Gimenez Attila Nemes Ruth Kerry Nathaniel A. Brunsell Cassandra J. WilsonAbstract
Soil macroporosity affects field-scale water-cycle processes, such as infiltration, nutrient transport and runoff1,2, that are important for the development of successful global strategies that address challenges of food security, water scarcity, human health and loss of biodiversity3. Macropores—large pores that freely drain water under the influence of gravity—often represent less than 1 per cent of the soil volume, but can contribute more than 70 per cent of the total soil water infiltration4, which greatly magnifies their influence on the regional and global water cycle. Although climate influences the development of macropores through soil-forming processes, the extent and rate of such development and its effect on the water cycle are currently unknown. Here we show that drier climates induce the formation of greater soil macroporosity than do more humid ones, and that such climate-induced changes occur over shorter timescales than have previously been considered—probably years to decades. Furthermore, we find that changes in the effective porosity, a proxy for macroporosity, predicted from mean annual precipitation at the end of the century would result in changes in saturated soil hydraulic conductivity ranging from −55 to 34 per cent for five physiographic regions in the USA. Our results indicate that soil macroporosity may be altered rapidly in response to climate change and that associated continental-scale changes in soil hydraulic properties may set up unexplored feedbacks between climate and the land surface and thus intensify the water cycle.
Abstract
In cold-temperate climate with high soil water content in spring, the farmer often faces the choice between topsoil compaction during seedbed preparation and delayed sowing, both of which may reduce attainable cereal yield. The objective of this study was to explore whether future climate change with increasing precipitation would aggravate this dilemma. We generated weather based on historical and projected future climate in Southeastern and Central Norway. Using this weather data as input, we simulated spring workability, attainable yield, timeliness costs, and mechanization management with a workability model and a mechanization model. The projected climate changes resulted in improved workability for spring fieldwork and higher attainable yield in South-eastern Norway, and either positive or negative changes in Central Norway compared to historical conditions. We observed a general increase in variability of workability and attainable yield, and a larger risk of extremely unfavourable years in the most unfavourable scenarios in Central Norway. Changes in profitability and mechanization management were small, but followed the same pattern. The negative effects in the most unfavourable climate scenarios in Central Norway were in contrast to positive effects in earlier studies. We explained discrepancies by differences in research methods and purpose. However, simulated sowing dates of annual crops should consider workability of the soil, in terms of water content. Under worst-case conditions, in need of a certain time window to complete their spring fieldwork, farmers might adapt to impaired spring workability by working the soil at higher water content than simulated in our study. The consequence would be a larger loss of attainable yield and less profitability in the future. We anticipate that negative effects may also be expected in other northern cold-temperate regions with high soil water content in spring.
Authors
Mohsen Ghanbari Vahid AkbariAbstract
Increased discrimination capability provided by polarimetric synthetic aperture radar (PolSAR) sensors compared to single and dual polarization synthetic aperture radar (SAR) sensors can improve land use monitoring and change detection. This necessitates reliable change detection methods in multitemporal PolSAR datasets. This paper proposes an unsupervised change detection algorithm for multilook PolSAR data. In the first step of the method, the Hotelling-Lawley trace (HLT) statistic is applied to measure the similarity of two multilook covariance matrices. As a result of this step, a scalar test statistic image is generated. Then, in the second step, a generalized Kittler and Illingworth (K&I) minimum-error thresholding algorithm is developed to perform on the test statistic image and discriminate between changed and unchanged areas. The K&I thresholding algorithm is based on the generalized Gamma distribution for statistical modeling of change and no-change classes. The proposed methodology is tested on a simulated PolSAR data and two C-band fully PolSAR datasets acquired by the uninhabited aerial vehicle SAR and RADARSAT-2 SAR satellites. The experiments show that the proposed algorithm accurately discriminates between change and no-change areas providing detection results with noticeably lower error rates and higher detection accuracy values compared to those of a CFAR-type thresholding of the HLT statistic. Also, the performance of the HLT statistic compared to the other statistics applied on the multilook polarimetric SAR data is assessed. Goodness-of-fit test results prove that the estimated generalized Gamma class conditional models adequately fit the corresponding change and no-change classes.
Authors
Daniela Franz Manuel Acosta Nuria Altimir Nicola Arriga Dominique Arrouays Marc Aubinet Mika Aurela Edward Ayres Ana López-Ballesteros Mireille Barbaste Daniel Berveiller Sébastien Biraud Hakima Boukir Timothy Brown Christian Brümmer Nina Buchmann George Burba Arnaud Carrara Allessandro Cescatti Eric Ceschia Robert Clement Edoardo Cremonese Patrick Crill Eva Darenova Sigrid Dengel Petra D'Odorico Gianluca Filippa Stefan Fleck Gerardo Fratini Roland Fuß Bert Gielen Sébastien Gogo John Grace Alexander Graf Achim Grelle Patrick Gross Thomas Grünwald Sami Haapanala Markus Hehn Bernard Heinesch Jouni Heiskanen Mathias Herbst Christine Herschlein Lukas Hörtnagl Koen Hufkens Andreas Ibrom Claudy Jolivet Lilian Joly Michael Jones Ralf Kiese Leif Klemedtsson Natascha Kljun Katja Klumpp Pasi Kolari Olaf Kolle Andrew Kowalski Werner Kutsch Tuomas Laurila Anne de Ligne Sune Linder Anders Lindroth Annalea Lohila Bernhard Longdoz Ivan Mammarella Tanguy Manise Sara Maraňón Jiménez Giorgio Matteucci Matthias Mauder Philip Meier Lutz Merbold Simone Mereu Stefan Metzger Mirco Migliavacca Meelis Mölder Leonardo Montagnani Christine Moureaux David Nelson Eiko Nemitz Giacomo Nicolini Mats B. Nilsson Maarten op de Beeck Bruce Osborne Mikaell Ottosson Löfvenius Marian Pavelka Matthias Peichl Olli Peltola Mari Pihlatie Andrea Pitacco Radek Pokorny Jukka Pumpanen Céline Ratié Corinna Rebmann Marilyn Roland Simone Sabbatini Nicolas P.A. Saby Matthew Saunders Hans Peter Schmid Marion Schrumpf Pavel Sedlák Penelope Serrano Ortiz Lukas Siebicke Ladislav Šigut Hanna Marika Silvennoinen Guillaume Simioni Ute Skiba Oliver Sonnentag Kamel Soudani Patrice Soulé Rainer Steinbrecher Tiphaine Tallec Anne Thimonier Eeva-Stiina Tuittila Juha-Pekka Tuovinen Patrik Vestin Gaëlle Vincent Caroline Vincke Domenico Vitale Peter Waldner Per Weslien Lisa Wingate Georg Wohlfahrt Mark Zahniser Timo VesalaAbstract
Research infrastructures play a key role in launching a new generation of integrated long-term, geographically distributed observation programmes designed to monitor climate change, better understand its impacts on global ecosystems, and evaluate possible mitigation and adaptation strategies. The pan-European Integrated Carbon Observation System combines carbon and greenhouse gas (GHG; CO2, CH4, N2O, H2O) observations within the atmosphere, terrestrial ecosystems and oceans. High-precision measurements are obtained using standardised methodologies, are centrally processed and openly available in a traceable and verifiable fashion in combination with detailed metadata. The Integrated Carbon Observation System ecosystem station network aims to sample climate and land-cover variability across Europe. In addition to GHG flux measurements, a large set of complementary data (including management practices, vegetation and soil characteristics) is collected to support the interpretation, spatial upscaling and modelling of observed ecosystem carbon and GHG dynamics. The applied sampling design was developed and formulated in protocols by the scientific community, representing a trade-off between an ideal dataset and practical feasibility. The use of open-access, high-quality and multi-level data products by different user communities is crucial for the Integrated Carbon Observation System in order to achieve its scientific potential and societal value.
Authors
Marian Pavelka Manuel Acosta Ralf Kiese Núria Altimir Christian Brümmer Patrick Crill Eva Darenova Roland Fuß Bert Gielen Alexander Graf Leif Klemedtsson Annalea Lohila Bernhard Longdoz Anders Lindroth Mats Nilsson Sara Maraňón Jiménez Lutz Merbold Leonardo Montagnani Matthias Peichl Mari Pihlatie Jukka Pumpanen Penelope Serrano Ortiz Hanna Marika Silvennoinen Ute Skiba Patrik Vestin Per Weslien Dalibor Janous Werner KutschAbstract
Chamber measurements of trace gas fluxes between the land surface and the atmosphere have been conducted for almost a century. Different chamber techniques, including static and dynamic, have been used with varying degrees of success in estimating greenhouse gases (CO2, CH4, N2O) fluxes. However, all of these have certain disadvantages which have either prevented them from providing an adequate estimate of greenhouse gas exchange or restricted them to be used under limited conditions. Generally, chamber methods are relatively low in cost and simple to operate. In combination with the appropriate sample allocations, chamber methods are adaptable for a wide variety of studies from local to global spatial scales, and they are particularly well suited for in situ and laboratory-based studies. Consequently, chamber measurements will play an important role in the portfolio of the Pan-European long-term research infrastructure Integrated Carbon Observation System. The respective working group of the Integrated Carbon Observation System Ecosystem Monitoring Station Assembly has decided to ascertain standards and quality checks for automated and manual chamber systems instead of defining one or several standard systems provided by commercial manufacturers in order to define minimum requirements for chamber measurements. The defined requirements and recommendations related to chamber measurements are described here.
Authors
Andre Acksel Luise Giani Carolin Stasch Peter Kühn Sebastian Eiter Kerstin Potthoff Tom Regier Peter LeinweberAbstract
Some previous studies showed that the formation of several deep dark humus-rich topsoils in Northern Europe was strongly influenced by the application of different organic materials by anthropogenic activities in former times. Such topsoils classified as plaggic Anthrosols also occurred in the Jæren region in SW Norway. However, source material and formation time of these Plaggic Anthrosols have not yet been clarified. Close to this region we found further humus-rich topsoils in the Karmøy municipality (2 sites at main island of Karmøy and 1 site at Feøy). These soils show a thick humus-rich topsoil up to 30 cm, and their formation cannot only be explained by natural conditions. We analyzed the molecular signature of the soil organic matter (SOM) by benzene polycarboxylic acids (BPCA), non-targeted bulk SOM mass spectrometry, δ34S and 14C AMS dating in order to determine source materials and the age of the SOM. The black carbon (BC) contents of the plaggic soils in Jæren (mean 3.4 g kg−1) deliver clear evidence for inputs of combustion residues from ancient fire management and/or from settlements. The C-XANES and Py-FIMS-spectra reveal relative enrichments of aromatic C and heterocyclic N compounds in the plaggic soils corresponding to the BC contents. In contrast, the humus-rich topsoils in Karmøy seem to be unaffected by fire management due to the low BC contents (mean 0.6 g kg−1) and the relative low portions of aromatic C and heterocyclic N compounds from C-XANES and Py-FIMS. The δ34S isotope signature of the SOM ranged from 10.6 to 15.2‰ in the soils at the islands and 10.0 to 13.5‰ in Jæren, corresponding to the Anthrosols in the Baltic Sea region (Median: δ34S = 11.5‰) and suggest an input of marine biomass (δ34S of seaweed = 20‰). The AMS 14C dating and complementary archaeological literature implied that the soils in Jæren and Karmøy have been formed between the Roman Iron Age (500 BC to AD 500) and the Viking Age (AD 800 to AD 1,000). Our results provide strong evidence for an anthropo-pedogenesis of the humus-rich topsoils in Karmøy and indicate parallels to the plaggic soils in Jæren as well as to Anthrosols in the Baltic Sea region. Therefore, we propose to classify the humus-rich topsoils in Karmøy as Anthrosols.
Authors
Ramón Fernando Colmenares-Quintero Luis-Fernando Latorre-Noguera Juan-Carlos Colmenares-Quintero Janka DibdiakovaAbstract
The study carried out here aims to determine the advantage of using in-situ electricity generation facilities versus conventional generators, being evaluated from the environmental point of view. For this, an environmental analysis on the production of CO2 has been applied to two scenarios of electricity generation for a residential building in Medellin city (Colombia). The first one refers to La Sierra thermo-electric plant located in La Sierra, municipality of Puerto Nare, in the Antioquiashire, which is the most efficient plant in Colombian thermal generation. The second comparison scenario refers to the annual operation of a micro-cogeneration facility, which satisfies the building's hot water and electrical energy needs. Using the capabilities of the TRNSYS v17® energy simulation software and the emission equations available in the public domain, the comparative environmental analysis is carried out between one and the other for the same load. The losses in electric transmission are assumed to be 10%. This analysis has shown a difference of more than 50% in emissions generation, with the main cause being the amount of fuel used, which for both cases is natural gas. On the other hand, this study shows the environmental advantages in the use of in-situ generators, decreasing transmission losses.
Abstract
The hydrological processes associated with vegetation and their effect on slope stability are complex and so difficult to quantify, especially because of their transient effects (e.g. changes throughout the vegetation life cycle). Additionally, there is very limited amount of field based research focusing on investigation of coupled hydrological and mechanical influence of vegetation on stream bank behavior, accounting for both seasonal time scale and different vegetation types, and none dedicated to marine clay soils (typically soil type for Norway). In order to fill this gap we established hydrological and mechanical monitoring of selected test plots within a stream bank, covered with different types of vegetation, typical for Norwegian agricultural areas (grass, shrubs and trees). The soil moisture, groundwater level and stream water level were continuously monitored. Additionally, soil porosity and shear strength were measured regularly. Observed hydrological trends and differences between three plots (grass, tree and shrub) were analysed and formed the input base for stream bank stability modeling. We did not find particular differences between the grass and shrub plot but we did observe a significantly lower soil moisture content, lower soil porosity and higher shear strength within the tree plot. All three plots were stable during the monitoring period, however modeling scenarios made it possible to analyse potential differences in stream bank stability under different vegetation cover depending on root reinforcement and slope angle.
Authors
Monika Suškevičs Sebastian Eiter Stanislav Martinat Dina Stober Elis Vollmer Cheryl L. de Boer Matthias BucheckerAbstract
The successful transition towards renewable energy (RE) technologies is closely intertwined with various societal aspects. Wind energy (WE) is one of the most controversial RE-types, possibly due to the multiplicity of related public concerns. Although some European country-comparisons exist, research concerning acceptance factors in different political and cultural planning contexts is scarce, especially in Eastern and Southern Europe. This paper explores the variation of (1) acceptance issues across Europe, and (2) patterns of strategic and local planning in affecting WE acceptance. We conducted an expert survey among the members of the COST Action ‘Renewable Energy and Landscape Quality’ and the association Wind Energy Europe. We found that acceptance issues – as perceived by the experts – across different regions in Europe share certain similarities, such as concerns about landscape impacts. The priority-levels of acceptance issues are specific to each region and link to the planning quality in that context. Planners’ and decision-makers’ increased awareness about the diversity of acceptance issues would allow them to design more appropriate strategic and local planning processes.
Authors
Tomas Persson Mats Höglind Marcel van Oijen Panu Korhonen Taru Palosuo Guillaume Jégo Perttu Virkajärvi Gilles Bélanger Anne-Maj GustavssonAbstract
Different forage grass models are used to simulate forage yield and nutritive attributes, but these models are seldom compared, particularly those for timothy (Phleum pratense L.), a widely grown forage grass species in agricultural regions with a cold temperate climate. We compared the models BASGRA, CATIMO and STICS for their predictions of timothy crude protein (CP) concentration, neutral detergent fibre (NDF) concentration and NDF digestibility (dNDF), three important forage nutritive attributes. Data on CP and NDF concentrations, and dNDF and the associated weather and soil data for seven cultivars, taken from eight field experiments in Canada, Finland, Norway, and Sweden, were divided into calibration and validation datasets. Model parameters were estimated for each cultivar separately (cultivar-specific calibration) and for all cultivars together (generic calibration), using different methods for the three models. Normalized root mean square error (RMSE) in prediction of CP concentration varied between 16 and 26% for BASGRA, 45 and 101% for CATIMO and 23 and 40% for STICS across the two calibration methods and the calibration and validation datasets. Normalised RMSE in prediction of NDF concentration varied between 8 and 13% for BASGRA, 14 and 21% for CATIMO and 8 and 12% for STICS, while for dNDF it varied between 7 and 22% for BASGRA, 7 and 38% for CATIMO and 5 and 6% for STICS. Cultivar-specific calibration improved the performance of CATIMO and STICS, but not BASGRA, compared with generic calibration. The prediction accuracy for NDF concentration and dNDF with the three models was within the same range or better than that for forage dry matter (DM) yield of timothy. Overall, the three models performed well in predicting some nutritive attributes and yield in Northern Europe and Canada, but improvements are required, particularly to increase the prediction accuracy of CP concentration.
Authors
Eivind Vangdal Angela KoortAbstract
The major sources of nutrients to organic grown apple trees are fertilizers made from manure, compost, bone meal, etc. Depending on humidity and temperature in soil and air, the nutrients are dissolved or mineralized and made available to the trees during the growing season. In conventional apple growing, the trees are given mineral fertilizers in early spring to improve the nitrogen status in the trees during flowering for better fruit set. Is it possible in an organic production system to increase the plant available nitrogen in the flowering period by application of liquid N-fertilizers? The standard fertilizer in Norwegian organic fruit growing is dried and pelleted chicken manure with bone meal and vinasse (Marihøne plus; NPK 8-4-5). In these experiments, a liquid fertilizer (Pioner Hi-fruit; NPK 4-1-5) based on vegetable matter plus potassium-vinasse was compared to the standard fertilizer. The liquid fertilizer was applied to the soil as fertigation from 2 weeks before the estimated start of flowering. The dry product was applied 2 weeks prior to flowering. To incorporate the fertilizers into the soil, a mechanical hoer (Orizzonti, Italy) was run in all plots after the application of dry fertilizer. The nitrogen and mineral contents in soil, leaves and fruit were analyzed. The liquid fertilization applied on the soil in the spring gave higher N-contents in soil and trees compared to the dried manure product. However, the increase in N-content was not very strong in the leaf samples. Apples from trees given high doses of liquid fertilizers were greener with less cover colour and higher IAD-indexes. Still they were softer and had less starch than fruit from other treatments.
Abstract
This research note offers a critical-constructive discussion of the article ‘Class, Culture and Culinary Tastes: Cultural Distinctions and Social Class Divisions in Contemporary Norway’, written by Flemmen, Hjellbrekke and Jarness (FHJ) (Sociology, 2018(1)). Concerns are raised about the methods and the use of the data. A robustness analysis with alternative data and/or alternative methods is suggested. Conceptually, the analysis of FHJ is considered not to engage adequately with a more qualitative body of historical and ethnological literature, as well as the impact of Norwegian agricultural policy. To describe and understand the evolution of social meaning and social patterns of the consumption of ‘traditional’ Norwegian foodstuffs, a qualitative approach could have contributed constructively. Overall, wider implications for Bourdieu-inspired analyses of cultural consumption are addressed.
Abstract
We describe Arge bella Wei & Du sp. nov., a large and beautiful species of Argidae from south China, and report its mitochondrial genome based on high-throughput sequencing data. We present the gene order, nucleotide composition of proteincoding genes (PCGs), and the secondary structures of RNA genes. The nearly complete mitochondrial genome of A. bella has a length of 15,576 bp and a typical set of 37 genes (22 tRNAs, 13 PCGs, and 2 rRNAs). Three tRNAs are rearranged in the A. bella mitochondrial genome as compared to the ancestral type in insects: trnM and trnQ are shuffled, while trnW is translocated from the trnW -trnC-trnY cluster to a location downstream of trnI. All PCGs are initiated by ATN codons, and terminated with TAA, TA or T as stop codons. All tRNAs have a typical cloverleaf secondary structure, except for trnS1. H821 of rrnS and H976 of rrnL are redundant. A phylogenetic analysis based on mitochondrial genome sequences of A. bella, 21 other symphytan species, two apocritan representatives, and four outgroup taxa supports the placement of Argidae as sister to the Pergidae within the symphytan superfamily Tenthredinoidea.
Abstract
Degradation of organic chemicals in natural soils depends on oxidation-reduction conditions. To protect our groundwater resources we need to understand the degradation processes under anaerobic conditions. Available iron and manganese oxides are used as electron acceptors for anaerobic degradation and are reduced to the dissolved form of metallic cations in pore water. To monitor this process is a challenge, because anaerobic conditions are difficult to sample directly without introducing oxygen. A few studies have shown an impact of iron reduction on spectral induced polarisation (SIP) signature, often associated with bacterial growth. Our objective is to study the impact of iron and manganese oxide dissolution, caused by degradation of an organic compound, with spectral induced polarisation signatures. Twenty-six vertical columns (30 cm high, inner diameter 4.6 cm) were filled with a sand rich in oxides (manganese and iron) with a static water table in the middle. In half of the columns, a 2 cm high contaminated layer was installed just above the water table. As the contaminant degrades, the initial oxygen is consumed and anaerobic conditions form Every three days over a period of one month, spectral induced polarisation (twenty frequencies between 5mHz and 10 kHz) data were collected on six columns: three contaminated replicates and three control replicates. Chemical analysis was done on twenty columns assigned for destructive water sampling, ten contaminated columns and ten control. The results show an increase of the real conductivity associated with the degradation processes, independent of frequency. Compared with the pore water electrical conductivity in the saturated zone, the real conductivity measurement revealed the formation of surface conductivity before iron was released in the pore water. In parallel, we also observed an evolution of the imaginary conductivity in both saturated and unsaturated zones at frequencies below 1 Hz. Overall, the anaerobic reduction of iron and manganese oxide during the organic degradation increased both the conductive and polarisation component of the complex conductivity.
Abstract
The ericaceous shrub bilberry (Vaccinium myrtillus L.) is a keystone species of the Eurasian boreal forest. The most optimal light condition for this plant is partial shading. Shade from the forest canopy depends on the stand density, a forest attribute that can be manipulated by forest managers. Most previous studies of the relationship between bilberry abundance and forest density have not explored the potentially modifying impacts of factors like stand age, tree species composition, and the solar irradiation at the site, as determined by location and topography. Using data from the Norwegian National Forest Inventory, we developed a generalized linear model applicable to estimate local bilberry cover across a wide range of environmental conditions in Norway. The explanatory terms in the final model were stand density (basal area per ha), solar irradiation, stand age, percentages of deciduous, pine, and spruce trees, summer (June-August) mean temperature and precipitation sum, mean temperature in January, site index, and soil category, in addition to the two-way interactions between stand density and the following: solar irradiation, stand age, percentage of deciduous trees, and percentage of Norway spruce (Picea abies). The final model explained ca. 21% of the total variation in bilberry cover. We conclude that a stand density of c. 30 m2 ha−1 in general will create favourable conditions for bilberry. If the forest is younger than 80 years old, or dominated by Norway spruce or deciduous trees, the optimal stand density is reduced to around 20 m2 ha−1. In a forest dominated by Scots pine (Pinus sylvestris), basal areas up to 40 m2 ha−1 would be beneficial to bilberry abundance. Our results demonstrate the importance of considering interactions between stand density and other stand and site characteristics.
Authors
Victor Phani Vishal S. Somvanshi Rohit N. Shukla Keith Davies Uma RaoAbstract
Background: Southern root-knot nematode Meloidogyne incognita (Kofoid and White, 1919), Chitwood, 1949 is a key pest of agricultural crops. Pasteuria penetrans is a hyperparasitic bacterium capable of suppressing the nematode reproduction, and represents a typical coevolved pathogen-hyperparasite system. Attachment of Pasteuria endospores to the cuticle of second-stage nematode juveniles is the first and pivotal step in the bacterial infection. RNA-Seq was used to understand the early transcriptional response of the root-knot nematode at 8 h post Pasteuria endospore attachment. Results: A total of 52,485 transcripts were assembled from the high quality (HQ) reads, out of which 582 transcripts were found differentially expressed in the Pasteuria endospore encumbered J2 s, of which 229 were up-regulated and 353 were down-regulated. Pasteuria infection caused a suppression of the protein synthesis machinery of the nematode. Several of the differentially expressed transcripts were putatively involved in nematode innate immunity, signaling, stress responses, endospore attachment process and post-attachment behavioral modification of the juveniles. The expression profiles of fifteen selected transcripts were validated to be true by the qRT PCR. RNAi based silencing of transcripts coding for fructose bisphosphate aldolase and glucosyl transferase caused a reduction in endospore attachment as compared to the controls, whereas, silencing of aspartic protease and ubiquitin coding transcripts resulted in higher incidence of endospore attachment on the nematode cuticle. Conclusions: Here we provide evidence of an early transcriptional response by the nematode upon infection by Pasteuria prior to root invasion. We found that adhesion of Pasteuria endospores to the cuticle induced a downregulated protein response in the nematode. In addition, we show that fructose bisphosphate aldolase, glucosyl transferase, aspartic protease and ubiquitin coding transcripts are involved in modulating the endospore attachment on the nematode cuticle. Our results add new and significant information to the existing knowledge on early molecular interaction between M. incognita and P. penetrans.
Authors
Ivan N. Bolotov Alexander A. Makhrov Mikhail Yu. Gofarov Olga V. Aksenova Paul Eric Aspholm Yulia V. Bespalaya Mikhail B. Kabakov Yulia S. Kolosova Alexander V. Kondakov Thomas Ofenböck Andrew N. Ostrovsky Igor Yu. Popov Ted von Proschwitz Mudite Rudzite Maris Rudzitis Svetlana E. Sokolova Ilmari Valovirta Ilya V. Vikhrev Maxim V. Vinarski Alexey A. ZotinAbstract
The effects of climate change on oligotrophic rivers and their communities are almost unknown, albeit these ecosystems are the primary habitat of the critically endangered freshwater pearl mussel and its host fishes, salmonids. The distribution and abundance of pearl mussels have drastically decreased throughout Europe over the last century, particularly within the southern part of the range, but causes of this wide-scale extinction process are unclear. Here we estimate the effects of climate change on pearl mussels based on historical and recent samples from 50 rivers and 6 countries across Europe. We found that the shell convexity may be considered an indicator of the thermal effects on pearl mussel populations under warming climate because it reflects shifts in summer temperatures and is significantly different in viable and declining populations. Spatial and temporal modeling of the relationship between shell convexity and population status show that global climate change could have accelerated the population decline of pearl mussels over the last 100 years through rapidly decreasing suitable distribution areas. Simulation predicts future warming-induced range reduction, particularly in southern regions. These results highlight the importance of large-scale studies of keystone species, which can underscore the hidden effects of climate warming on freshwater ecosystems.
Authors
Gregory Taff Yang Shao Jie Ren Ruoyu ZhangAbstract
The accuracy in land-cover classification using remotely sensed imagery can be increased using Bayesian methods that incorporate prior probabilities of classes. However, estimating these prior probabilities can be expensive and data intensive. We propose methods to improve the classification accuracy using Bayesian methods to classify ambiguous (or low-confidence) pixels, using only the remotely sensed imagery or existing land-cover maps to estimate prior probabilities. We propose a spatial method that predicts prior probabilities from the original image, and a temporal method that incorporates land-cover maps from previous years. We illustrate our methods with a neural network (NN) classifier on the U.S. state of Iowa to classify crops into corn/soybean/other using moderate resolution imaging spectroradiometer (MODIS) data. USDA cropland data layers were aggregated to the 250-m resolution of MODIS and used as ground truth, based on a cropland mask from the National Land Cover Database. Results show that the spatial-prior-adjustment method, which predicts prior probabilities for low-confidence pixels based on class percentages of initial NN classification, increased overall accuracy of low-confidence pixels between 2% and 3.3% over the standard NN classification. The temporal-prior-adjustment method, which uses crop classes from the previous six years to estimate prior probabilities for the current year, shows significantly greater accuracy improvement for low-confidence pixels (almost 7%) over the standard NN classification. Increased benefit of the temporal-prior-adjustment method relative to the spatial-prior-adjustment method is likely due to increased information from more ground truth data (from previous years) than the spatial method.
Authors
Francesca Giampieri Sadia Afrin Derek Stewart Gordon J. McDougall Rex Brennan Lesley Blyth Massimiliano Gasparrini Luca Mazzoni Franco Capocasa José Miguel Alvarez-Suarez Stefano Bompadre Pedro Nogueira Brás de Oliveira Claudia N. Santos Manuel Masias Pablo Agudo Jorge Crespo Bruno Mezzetti Tamara Y. Forbes-Hernández Maurizio BattinoAbstract
Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p < 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion.
Authors
Monica Suarez Korsnes Reinert KorsnesAbstract
Long-term video-based tracking of single A549 lung cancer cells exposed to three different concentrations of the marine toxin yessotoxin (YTX) reveals significant variation in cytotoxicity, and it confirms the potential genotoxic effects of this toxin. Tracking of single cells subject to various toxic exposure, constitutes a conceptually simple approach to elucidate lineage correlations and sub-populations which are masked in cell bulk analyses. The toxic exposure can here be considered as probing a cell population for properties and change which may include long-term adaptation to treatments. Ranking of pedigree trees according to a measure of “size,” provides definition of sub-populations. Following single cells through generations indicates that signaling cascades and experience of mother cells can pass to their descendants. Epigenetic factors and signaling downstream lineages may enhance differences between cells and partly explain observed heterogeneity in a population. Signaling downstream lineages can potentially link a variety of observations of cells making resulting data more suitable for computerized treatment. YTX exposure of A549 cells tends to cause two main visually distinguishable classes of cell death modalities (“apoptotic-like” and “necrotic-like”) with approximately equal frequency. This special property of YTX enables estimation of correlation between cell death modalities for sister cells indicating impact downstream lineages. Hence, cellular responses and adaptation to treatments might be better described in terms of effects on pedigree trees rather than considering cells as independent entities.
Authors
Ahmad Hamidov Katharina Helming Gianni Bellocchi Waldemar Bojar Tommy Dalgaard Bhim Bahadur Ghaley Christian Hoffmann Ian Holman Annelie Holzkämper Dominika Krzeminska Sigrun Hjalmarsdottir Kværnø Heikki Lehtonen Georg Niedrist Lillian Øygarden Pytrik Reidsma Pier Paolo Roggero Teodor Rusu Cristina Santos Giovanna Seddaiu Eva Skarbøvik Domenico Ventrella Jacek Żarski Martin SchönhartAbstract
Soils are vital for supporting food security and other ecosystem services. Climate change can affect soil functions both directly and indirectly. Direct effects include temperature, precipitation, and moisture regime changes. Indirect effects include those that are induced by adaptations such as irrigation, crop rotation changes, and tillage practices. Although extensive knowledge is available on the direct effects, an understanding of the indirect effects of agricultural adaptation options is less complete. A review of 20 agricultural adaptation case‐studies across Europe was conducted to assess implications to soil threats and soil functions and the link to the Sustainable Development Goals (SDGs). The major findings are as follows: (a) adaptation options reflect local conditions; (b) reduced soil erosion threats and increased soil organic carbon are expected, although compaction may increase in some areas; (c) most adaptation options are anticipated to improve the soil functions of food and biomass production, soil organic carbon storage, and storing, filtering, transforming, and recycling capacities, whereas possible implications for soil biodiversity are largely unknown; and (d) the linkage between soil functions and the SDGs implies improvements to SDG 2 (achieving food security and promoting sustainable agriculture) and SDG 13 (taking action on climate change), whereas the relationship to SDG 15 (using terrestrial ecosystems sustainably) is largely unknown. The conclusion is drawn that agricultural adaptation options, even when focused on increasing yields, have the potential to outweigh the negative direct effects of climate change on soil degradation in many European regions.
Authors
Ravishankar Narayana Judith Fliegmann Ivan Paponov Massimo E. MaffeiAbstract
The Earth magnetic field (or geomagnetic field, GMF) is a natural component of our planet and variations of the GMF are perceived by plants with a still uncharacterized magnetoreceptor. The purpose of this work was to assess the effect of near null magnetic field (NNMF, ∼40 nT) on Arabidopsis thaliana Col0 root ion modulation. A time-course (from 10 min to 96 h) exposure of Arabidopsis to NNMF was compared to GMF and the content of some cations (NH4 +, K+, Ca2+ and Mg2+) and anions (Cl−, SO4 =, NO3 − and PO4 =) was evaluated by capillary electrophoresis. The expression of several cation and anion channel- and transporter-related genes was assessed by gene microarray. A few minutes after exposure to NNMF, Arabidopsis roots responded with a significant change in the content and gene expression of all nutrient ions under study, indicating the presence of a plant magnetoreceptor that responds immediately to MF variations by modulating channels, transporters and genes involved in mineral nutrition. The response of Arabidopsis to reduced MF was a general reduction of plant ion uptake and transport. Our data suggest the importance to understand the nature and function of the plant magnetoreceptor for future space programs involving plant growth in environments with a reduced MF.
Authors
Olav Aaseth Hegnar Dejan Petrovic Bastien Bissaro Gry Alfredsen Aniko Varnai Vincent EijsinkAbstract
Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that perform oxidative cleavage of recalcitrant polysaccharides. We have purified and characterized a recombinant family AA9 LPMO, LPMO9B, from Gloeophyllum trabeum (GtLPMO9B) which is active on both cellulose and xyloglucan. Activity of the enzyme was tested in the presence of three different reductants: ascorbic acid, gallic acid, and 2,3-dihydroxybenzoic acid (2,3-DHBA). Under standard aerobic conditions typically used in LPMO experiments, the first two reductants could drive LPMO catalysis whereas 2,3-DHBA could not. In agreement with the recent discovery that H2O2 can drive LPMO catalysis, we show that gradual addition of H2O2 allowed LPMO activity at very low, substoichiometric (relative to products formed) reductant concentrations. Most importantly, we found that while 2,3-DHBA is not capable of driving the LPMO reaction under standard aerobic conditions, it can do so in the presence of externally added H2O2. At alkaline pH, 2,3-DHBA is able to drive the LPMO reaction without externally added H2O2, and this ability overlaps entirely the endogenous generation of H2O2 by GtLPMO9B-catalyzed oxidation of 2,3-DHBA. These findings support the notion that H2O2 is a cosubstrate of LPMOs and provide insight into how LPMO reactions depend on, and may be controlled by, the choice of pH and reductant.
Authors
Jannis von Buttlar Jakob Zscheischler Anja Rammig Sebastian Sippel Markus Reichstein Alexander Knohl Martin Jung Olaf Menzer M. Altaf Arain Nina Buchmann Alessandro Cescatti Damiano Gianelle Gerard Kiely Beverly E. Law Vincenzo Magliulo Hank Margolis Harry McCaughey Lutz Merbold Mirco Migliavacca Leonardo Montagnani Walter Oechel Marian Pavelka Matthias Peichl Serge Rambal Antonio Raschi Russell L. Scott Francesco P. Vaccari Eva van Gorsel Andrej Varlagin Georg Wohlfahrt Miguel D. MahechaAbstract
Extreme climatic events, such as droughts and heat stress, induce anomalies in ecosystem–atmosphere CO2 fluxes, such as gross primary production (GPP) and ecosystem respiration (Reco), and, hence, can change the net ecosystem carbon balance. However, despite our increasing understanding of the underlying mechanisms, the magnitudes of the impacts of different types of extremes on GPP and Reco within and between ecosystems remain poorly predicted. Here we aim to identify the major factors controlling the amplitude of extreme-event impacts on GPP, Reco, and the resulting net ecosystem production (NEP). We focus on the impacts of heat and drought and their combination. We identified hydrometeorological extreme events in consistently downscaled water availability and temperature measurements over a 30-year time period. We then used FLUXNET eddy covariance flux measurements to estimate the CO2 flux anomalies during these extreme events across dominant vegetation types and climate zones. Overall, our results indicate that short-term heat extremes increased respiration more strongly than they downregulated GPP, resulting in a moderate reduction in the ecosystem's carbon sink potential. In the absence of heat stress, droughts tended to have smaller and similarly dampening effects on both GPP and Reco and, hence, often resulted in neutral NEP responses. The combination of drought and heat typically led to a strong decrease in GPP, whereas heat and drought impacts on respiration partially offset each other. Taken together, compound heat and drought events led to the strongest C sink reduction compared to any single-factor extreme. A key insight of this paper, however, is that duration matters most: for heat stress during droughts, the magnitude of impacts systematically increased with duration, whereas under heat stress without drought, the response of Reco over time turned from an initial increase to a downregulation after about 2 weeks. This confirms earlier theories that not only the magnitude but also the duration of an extreme event determines its impact. Our study corroborates the results of several local site-level case studies but as a novelty generalizes these findings on the global scale. Specifically, we find that the different response functions of the two antipodal land–atmosphere fluxes GPP and Reco can also result in increasing NEP during certain extreme conditions. Apparently counterintuitive findings of this kind bear great potential for scrutinizing the mechanisms implemented in state-of-the-art terrestrial biosphere models and provide a benchmark for future model development and testing.
Authors
Carrie Joy Andrew Einar Heegaard Alan C. Gange Beatrice Senn-Irlet Simon Egli Paul M. Kirk Ulf Büntgen Håvard Kauserud Lynne BoddyAbstract
As citizen science and digitization projects bring greater and larger datasets to the scientific realm, wemust address the comparability of results across varying sources and spatial scales. Independentlyassembled fungal fruit body datasets from Switzerland and the UK were available at large, national-scales and more intensively surveyed, local-scales. Phenology responses of fungi between these data-sets at different scales (national, intermediate and local) resembled one another. Consistently with time,the fruiting season initiated earlier and extended later. Phenology better correlated across data sourcesand scales in the UK, which contain less landscape and environmental heterogeneity than Switzerland.Species-specific responses in seasonality varied more than overall responses, but generally fruiting startdates were later for most Swiss species compared with UK species, while end dates were later for both.The coherency of these results, across the data sources, supports the use of presence-only data obtainedby multiple recorders, and even across heterogeneous landscapes, for global change phenology research.
Authors
Rene Schils Jørgen E. Olesen Kurt-Christian Kersebaum Bert Rijk Michael Oberforster Valery Kalyada Maksim Khitrykau Anne Gobin Hristofor Kirchev Vanya Manolova Ivan Manolov Mirek Trnka Petr Hlavinka Taru Palosuo Pirjo Peltonen-Sainio Lauri Jauhiainen Josiane Lorgeou Helene Marrou Nikos Danalatos Sotirios Archontoulis Nandor Fodor John Spink Pier Paolo Roggero Simona Bassu Antonio Pulina Till Seehusen Anne Kjersti Uhlen Katarzyna Zylowska Anna Nierobca Jerzy Kozyra Joao Vasco Silva Benvindo Martins Macas José Coutinho Viorel Ion Jozef Takác M. Ines Minguez Henrik Eckersten Lilia Levy Juan Manuel Herrera Jürg Hiltbrunner Oleksii Kryvobok Oleksandr Kryvoshein Roger Sylvester-Bradley Daniel Kindred Cairistiona F.E. Topp Hendrik Boogaard Hugo de Groot Jan Peter Lesschen Lenny van Bussel Joost Wolf Mink Zijlstra Marloes P. van Loon Martin K. van IttersumAbstract
Europe accounts for around 20% of the global cereal production and is a net exporter of ca. 15% of that production. Increasing global demand for cereals justifies questions as to where and by how much Europe’s production can be increased to meet future global market demands, and how much additional nitrogen (N) crops would require. The latter is important as environmental concern and legislation are equally important as production aims in Europe. Here, we used a country-by-country, bottom-up approach to establish statistical estimates of actual grain yield, and compare these to modelled estimates of potential yields for either irrigated or rainfed conditions. In this way, we identified the yield gaps and the opportunities for increased cereal production for wheat, barley and maize, which represent 90% of the cereals grown in Europe. The combined mean annual yield gap of wheat, barley, maize was 239 Mt, or 42% of the yield potential. The national yield gaps ranged between 10 and 70%, with small gaps in many north-western European countries, and large gaps in eastern and south-western Europe. Yield gaps for rainfed and irrigated maize were consistently lower than those of wheat and barley. If the yield gaps of maize, wheat and barley would be reduced from 42% to 20% of potential yields, this would increase annual cereal production by 128 Mt (39%). Potential for higher cereal production exists predominantly in Eastern Europe, and half of Europe’s potential increase is located in Ukraine, Romania and Poland. Unlocking the identified potential for production growth requires a substantial increase of the crop N uptake of 4.8 Mt. Across Europe, the average N uptake gaps, to achieve 80% of the yield potential, were 87, 77 and 43 kg N ha−1 for wheat, barley and maize, respectively. Emphasis on increasing the N use efficiency is necessary to minimize the need for additional N inputs. Whether yield gap reduction is desirable and feasible is a matter of balancing Europe’s role in global food security, farm economic objectives and environmental targets.
Authors
Wei He Weimin Ju Christopher R. Schwalm Sebastian Sippel Xiaocui Wu Qiaoning He Lian Song Chunhua Zhang Jing Li Stephen Sitch Nicolas Viovy Pierre Friedlingstein Atul K. JainAbstract
Recently, severe droughts that occurred in North America are likely to have impacted its terrestrial carbon sink. However, process‐based understanding of how meteorological conditions prior to the onset of drought, for instance warm or cold springs, affect drought‐induced carbon cycle effects remains scarce. Here we assess and compare the response of terrestrial carbon fluxes to summer droughts in 2011 and 2012 characterized by contrasting spring conditions. The analysis is based on a comprehensive ensemble of carbon cycle models, including FLUXCOM, TRENDY v5, SiBCASA, CarbonTracker Europe, and CarbonTracker, and emerging Earth observations. In 2011, large reductions of net ecosystem production (NEP; −0.24 ± 0.17 Pg C/year) are due to decreased gross primary production (−0.17 ± 0.18 Pg C/year) and slightly increased ecosystem respiration (+0.07 ± 0.17 Pg C/year). Conversely, in 2012, NEP reductions (−0.17 ± 0.25 Pg C/year) are attributed to a larger increase of ecosystem respiration (+0.48 ± 0.27 Pg C/year) than gross primary production (+0.31 ± 0.29 Pg C/year), induced predominantly by an extra warmer spring prior to summer drought. Two temperate ecoregions crops/agriculture and the grass/shrubs contribute largest to these reductions and also dominate the interannual variations of NEP during 2007–2014. Moreover, the warming spring compensated largely the negative carbon anomaly due to summer drought, consistent with earlier studies; however, the compensation occurred only in some specific ecoregions. Overall, our analysis offers a refined view on recent carbon cycle variability and extremes in North America. It corroborates earlier results but also highlights differences with respect to ecoregion‐specific carbon cycle responses to drought and heat.
Authors
C.A. Baroffio L. Sigsgaard E.J. Ahrenfeldt Anna-Karin Borg-Karlson Sara Andrea Bruun J.V. Cross M.T. Fountain D. Hall R. Mozuraitis B. Ralle Nina Trandem Atle WibeAbstract
Most horticultural crops are attacked by more than one insect pest. As broad-spectrum chemical control options are becoming increasingly restricted, there is a need to develop novel control methods. Semiochemical attrac- tants are available for three important horticultural pests, strawberry blossom weevil, Anthonomus rubi Herbst (Coleoptera: Curculionidae), European tarnished plant bug, Lygus rugulipennis Poppius (Hemiptera: Miridae) and raspberry beetle, Byturus tomentosus deGeer (Coleoptera: Byturidae). Traps targeting more than one pest species would be more practical and economical for both monitoring and mass trapping than traps for single-species. In this study we aimed to (1) improve the effectiveness of existing traps for insect pests in strawberry and raspberry crops by increasing catches of each species, and (2) test if attractants for two unrelated pest species could be combined to capture both in the same trap without decreasing the total catches. Field tests were carried out in four European countries and different combinations of semiochemicals were compared. A volatile from straw- berry flowers, 1,4 dimethoxybenzene (DMB), increased the attractiveness of the aggregation pheromone to both sexes of A. rubi. The host-plant volatile, phenylacetaldehyde (PAA), increased the attraction of female L. rugu- lipennis to the sex pheromone, and, in strawberry, there was some evidence that adding DMB increased catches further. Traps baited with the aggregation pheromone of A. rubi, DMB, the sex pheromone of L. rugulipennis and PAA attracted both target species to the same trap with no significant difference in catches compared to those single-species traps. In raspberry, catches in traps baited with a combination of A. rubi aggregation pheromone, DMB and the commercially available lure for B. tomentosus, based on raspberry flower volatiles, were similar to those in single-species traps. In both crops the efficiency of the traps still needs improvement, but the multi- species traps are adequate for monitoring and should not lead to confusion for the user as the target species are easy to distinguish from each other.
Abstract
The long history of human land use have had a strong influence on ecosystems and landscapes in the boreal forest region of Northern Europe and created semi-natural habitats of high conservation value. In this study, we quantify land-cover change and loss of semi-natural grassland in an agricultural landscape (6.2 km2 ) in the boreal region of Norway from 1960 to 2015, and document a 49.1% loss of area that was seminatural grassland in 1960. The remaining semi-natural grasslands became smaller and the connectivity between them decreased. Intensification and abandonment of agricultural land use were of approximately equal importance for the loss of semi-natural grassland although the relative contribution of these processes depended on the topography and distance to farmsteads. The study provides an example of how change in land cover can be estimated and key drivers identified on a scale that is relevant for implementation of management and conservation measures.
Abstract
Chipping of bulky biomass assortments at roadside landings is a common and costly step in the biomass-to-energy supply chain. This operation normally involves one chipping unit and one or several transport trucks working together for simultaneous chipping and chip transport to a terminal or end user. Reducing the delay factors in these operations is a relevant ambition for lowering supply costs. A method to estimate organizational delay based on: (1) the capacity ratio between the transport and the chipper, (2) the use of buffer storage, and (3) the number of transport units involved is suggested here. Other delays will also be present, and some of these may relate to the working conditions at the landing. A method to set a landing functionality index based on characteristics of the forest landing is also suggested. A total of 14 roadside chipping operations were assessed and the operators were interviewed to address the impact of machinery configuration and landing characteristics on machine utilization. At most sites, the chipper was the more productive part, and the chipper utilization was to a large extent limited by organizational delay. Still the utilization of the transport units varied between 37 and 97%, of which some 36% of the variation was explained by the landing functionality index. Knowledge from the work presented here should be a good starting point for improving biomass supply planning and supply chain configuration.
Authors
Dominique Gravel Benjamin Baiser Jennifer A. Dunne Jens-Peter Kopelke Neo D. Martinez Tommi Nyman Timothee Poisot Daniel B. Stouffer Jason M. Tylianakis Spencer A. Wood Tomas RoslinAbstract
Biogeography has traditionally focused on the spatial distribution and abundance of species. Both are driven by the way species interact with one another, but only recently community ecologists realized the need to document their spatial and temporal variation. Here, we call for an integrated approach, adopting the view that community structure is best represented as a network of ecological interactions, and show how it translates to biogeography questions. We propose that the ecological niche should encompass the effect of the environment on species distribution (the Grinnellian dimension of the niche) and on the ecological interactions among them (the Eltonian dimension). Starting from this concept, we develop a quantitative theory to explain turnover of interactions in space and time – i.e. a novel approach to interaction distribution modeling. We apply this framework to host–parasite interactions across Europe and find that two aspects of the environment (temperature and precipitation) exert a strong imprint on species co-occurrence, but not on species interactions. Even where species co-occur, interaction proves to be stochastic rather than deterministic, adding to variation in realized network structure. We also find that a large majority of host-parasite pairs are never found together, thus precluding any inferences regarding their probability to interact. This first attempt to explain variation of network structure at large spatial scales opens new perspectives at the interface of species distribution modeling and community ecology.
Authors
Eva Lieungh Eriksen Heidrun Asgeirsdatter Ullerud Rune Halvorsen Sigrun Aune Harald Bratli Peter Horvath Inger Kristine Volden Anders Kvalvåg Wollan Anders BrynAbstract
Questions: Substantial variation between observers has been found when comparing parallel land-cover maps, but how can we know which map is better? What magnitude of error and inter-observer variation is expected when assigning land-cover types and is this affected by the hierarchical level of the type system, observer characteristics, and ecosystem properties? Study area: Hvaler, south-east Norway. Methods: Eleven observers assigned mapping units to 120 stratified random points. At each observation point, the observers first assigned a mapping unit to the point independently. The group then decided on a ‘true’ reference mapping unit for that point. The reference was used to estimate total error. ‘Ecological distance’ to the reference was calculated to grade the errors. Results: Individual observers frequently assigned different mapping units to the same point. Deviating assignments were often ecologically close to the reference. Total error, as percentage of assignments that deviated from the reference, was 35.0% and 16.4% for low and high hierarchical levels of the land-covertype system, respectively. The corresponding figures for inter-observer variation were 42.8% and 19.4%, respectively. Observer bias was found. Particularly high error rates were found for land-cover types characterised by human disturbance. Conclusions: Access to a ‘true’ mapping unit for each observation point enabled estimation of error in addition to the inter-observer variation typically estimated by the standard pairwise comparisons method for maps and observers. Three major sources of error in the assignment of land-cover types were observed: dependence on system complexity represented by the hierarchical level of the land-cover-type system, dependence on the experience and personal characteristics of the observers, and dependence on properties of the mapped ecosystem. The results support the necessity of focusing on quality in land-cover mapping, among commissioners, practitioners and other end users.
Authors
Tao Zhao Dineshkumar Kandasamy Paal Krokene Jingyuan Chen Jonathan Gershenzon Almuth HammerbacherAbstract
The bark beetle Ips typographus carries numerous fungi that could be assisting the beetle in colonizing live Norway spruce (Picea abies) trees. Phenolic defenses in spruce phloem are degraded by the beetle's major tree-killing fungus Endoconidiophora polonica, but it is unknown if other beetle associates can also catabolize these compounds. We compared the ability of five fungi commonly associated with I. typographus to degrade phenolic compounds in Norway spruce phloem. Grosmannia penicillata and Grosmannia europhioides were able to degrade stilbenes and flavonoids faster than E. polonica and grow on minimal growth medium with spruce bark constituents as the only nutrients. Furthermore, beetles avoided medium amended with phenolics but marginally preferred medium colonized by fungi. Taken together our results show that different bark beetle-associated fungi have complementary roles in degrading host metabolites and thus might improve this insect's persistence in well defended host tissues.
Authors
Piotr Goliński Marek Czerwiński Marit Jørgensen Jørgen A.B. Mølmann Barbara Golińska Gregory TaffAbstract
We investigated climatic trends in two contrasting locations in Europe at a regional level and at two specific sites, and we analysed how these trends are associated with the dry matter yield (DMY) of agriculturally improved grasslands. Trends of different meteorological variables were evaluated for Wielkopolska province, central Poland (1985-2014) and Troms county, northern Norway (1989-2015), as well as for two research stations located in these regions. Significant trends of increased mean air temperatures annually, and in April, June, July, August and November were identified both at the regional and site levels in Wielkopolska. In addition, growing degree days were increasing in Wielkopolska. In Troms, the common trends for the region and site studied were increase in mean air temperature in May and decrease in January. Grassland DMY was subsequently regressed against those meteorological variables for which significant trends were detected. In the Wielkopolska region, yields were negatively associated with the increase in air temperature in June, August, and the annual air temperature. The last relationship was also detected at the site level. We did not find any significant effects of climate trends on grassland DMY in the Norwegian study site or region.
Abstract
This paper presents an optimization model designed to find productivity functions for timber forwarding. Timber forwarding or skidding has for some 25 years been calculated using shortest path formulations on grid networks. Unfortunately, few productivity studies relate to such grids. Here, an inverse shortest path problem is presented, basically panning out costs on the grid based on point cost estimates. The formulation is tested using point cost estimates from the national forest inventories of Norway, together with a terrain model and other public spatial data (e.g. roads, water). The problem is optimized using the metaheuristic variable neighborhood search. The results of the test cases were achieved in reasonable time, and indicate that part of the solution space might be convex. The productivity function found for one of the test cases was used to create a variable forwarding cost map of the case area.
Authors
Andre van Eerde Johanna Gottschamel Ralph Bock Kristine Eraker Aasland Hansen Hetron Mweemba Munangandu Henry Daniell Jihong Liu ClarkeAbstract
Dengue fever is a mosquito (Aedes aegypti) ‐transmitted viral disease that is endemic in more than 125 countries around the world. There are four serotypes of the dengue virus (DENV 1‐4) and a safe and effective dengue vaccine must provide protection against all four serotypes. To date, the first vaccine, Dengvaxia (CYD‐TDV), is available after many decades’ efforts, but only has moderate efficacy. More effective and affordable vaccines are hence required. Plants offer promising vaccine production platforms and food crops offer additional advantages for the production of edible human and animal vaccines, thus eliminating the need for expensive fermentation, purification, cold storage and sterile delivery. Oral vaccines can elicit humoral and cellular immunity via both the mucosal and humoral immune systems. Here, we report the production of tetravalent EDIII antigen (EDIII‐1‐4) in stably transformed lettuce chloroplasts. Transplastomic EDIII‐1‐4‐expressing lettuce lines were obtained and homoplasmy was verified by Southern blot analysis. Expression of EDIII‐1‐4 antigens was demonstrated by immunoblotting, with the EDIII‐1‐4 antigen accumulating to 3.45% of the total protein content. Immunological assays in rabbits showed immunogenicity of EDIII‐1‐4. Our in vitro gastrointestinal digestion analysis revealed that EDIII‐1‐4 antigens are well protected when passing through the oral and gastric digestion phases but underwent degradation during the intestinal phase. Our results demonstrate that lettuce chloroplast engineering is a promising approach for future production of an affordable oral dengue vaccine.
Authors
Marie-Laure Desprez-Loustau Marie Massot Maude Toïgo Tania Fort Ayse Gülden Aday Kaya Johanna Boberg Uwe Braun Xavier Capdevielle Thomas Cech Anne Chandelier Petya Christova Tamara Corcobado Tugba Dogmus Cyril Dutech Olivier Fabreguettes Julie Faivre d'Arcier Andrin Gross Marilia Horta Jung Eugenia Iturritxa Thomas Jung Corina Junker Levente Kiss Kaloyan Kostov Asko Lehtijarvi Aneta Lyubenova Benoit Marçais Jonas Oliva Funda Oskay Martin Pastirčák Katarína Pastircakova Dominique Piou Gilles Saint-Jean Arnaud Sallafranque Slavtcho Slavov Jan Stenlid Venche Talgø Susumu Takamatsu Ayco JM. TackAbstract
The spatial distribution and niche differentiation of three closely related species (Erysiphe alphitoides, Erysiphe quercicola and Erysiphe hypophylla) causing oak powdery mildew was studied at scales ranging from the European continent, where they are invasive, to a single leaf. While E. alphitoides was dominant at all scales, E. quercicola and E. hypophylla had restricted geographic, stand and leaf distributions. The large-scale distributions were likely explained by climatic factors and species environmental tolerances, with E. quercicola being more frequent in warmer climates and E. hypophylla in colder climates. The extensive sampling and molecular analyses revealed the cryptic invasion of E. quercicola in nine countries from which it had not previously been recorded. The presence of the three species was also strongly affected by host factors, such as oak species and developmental stage. Segregation patterns between Erysiphe species were observed at the leaf scale, between and within leaf surfaces, suggesting competitive effects.
Authors
Habtamu AlemAbstract
Previous studies estimating TFP and its components have been criticized for not considering farm heterogeneity in their model. Moreover, the studies focused on the technical evaluation of a sector. However, the technical evaluation alone reveals how well farmers use the physical production process. There is a need to closely examine the cost efficiency of the farmers. In this study, we used a cost function (dual) approach to facilitating the decomposition and estimation of TFP components. Using a translog stochastic cost function, we estimated the level and source of productivity and profitability change for crop producing family firms in Norway. We used the true random effect to account for farm heterogeneity. The analysis is based on 23 years unbalanced panel data (1991-2013) from 455 crop- producing firms with a total of 3885 observations. The result indicates that average annual productivity growth rate in grain and forage production was - 0.11 % per annum during the period 1991-2013. The profit change was 0.14 % per annum.
Authors
Zbyněk Hrkal Ketil Haarstad David Rozman Jan Těšitel Drahomira Kušová Eva Novotná Miroslav VáňaAbstract
The fast development of laboratory methods has revealed increased amounts of trace concentrations of pharmaceuticals and personal care products (PPCP) in waste waters in the Czech Republic. This paper focuses on the expected costs to solve this problem by quaternary treatment of waste water based on activated carbon filtration. The one-time investment costs in 155 wastewater treatment plants (WWTPs) with a capacity of over 10 000 population equivalent (PE) would represent an amount of around 300 million EUR. The increase in end-user operat-ing costs would be 0.4 EUR/m3, which would mean a 15% increase in water and sewage costs. A sociological survey showed that most respondents (65%) would agree with an increase in price but only by 10%. Currently the cost of the qua-ternary treatment of wastewater is based primarily on estimates. Therefore changes in legislation leading to stricter limits and an increase in the efficiency of wastewater treatment should be preceded by additional applied research.
Authors
Anna Bucharova Oliver Bossdorf Norbert Hölzel Johannes Kollmann Rüdiger Prasse Walter DurkaAbstract
One of the main questions in ecosystem restoration is where to obtain the seeds to re-establish plant communities. While the most commonly advocated approach is to use seeds from local sources, some experts argue against this because local populations may harbour little genetic variability for the restored populations to be able to adapt to and survive global change. Instead, they propose alternative strategies such as mixing seeds from various sources to increase genetic variability and adaptive potential, or using seeds from populations that have a similar climate as predicted for the target locality in the future. All these alternative seed-sourcing strategies have in common that they involve a transplanting of plant ecotypes, sometimes over large spatial scales. This is risky because plants from distant origins may be maladapted to the current local abiotic and biotic environment. In addition, introduction of non-local provenances will disrupt natural patterns of withinspecies biodiversity and will affect ecological networks, with unpredictable consequences. To balance the value of local adaptation with the need for future adaptation potential, we propose ‘regional admixture provenancing’ as a compromise strategy. Here seeds are sourced from multiple populations within the same region as the target locality and mixed prior to use. The mixing of seeds will increase the genetic diversity necessary for future adaptation, while restricting seed origins to a regional scale will maintain regional adaptation and reduce the risk of unintended effects on other biota. This approach is feasible in practice and has recently been implemented in Germany. We believe that it represents a compromise to reconcile opposing views on ecological restoration.
Abstract
No abstract has been registered
Authors
Mart-Jan Schelhaas Geerten M. Hengeveld Nanny Heidema Esther Thürig Brigitte Rohner Giorgio Vacchiano Jordi Vayreda John Redmond Jaroslaw Socha Jonas Fridman Stein Michael Tomter Heino Polley Susana Barreiro Gert-Jan NabuursAbstract
Background: Over the last decades, many forest simulators have been developed for the forests of individual European countries. The underlying growth models are usually based on national datasets of varying size, obtained from National Forest Inventories or from long-term research plots. Many of these models include country- and location-specific predictors, such as site quality indices that may aggregate climate, soil properties and topography effects. Consequently, it is not sensible to compare such models among countries, and it is often impossible to apply models outside the region or country they were developed for. However, there is a clear need for more generically applicable but still locally accurate and climate sensitive simulators at the European scale, which requires the development of models that are applicable across the European continent. The purpose of this study is to develop tree diameter increment models that are applicable at the European scale, but still locally accurate. We compiled and used a dataset of diameter increment observations of over 2.3 million trees from 10 National Forest Inventories in Europe and a set of 99 potential explanatory variables covering forest structure, weather, climate, soil and nutrient deposition. Results: Diameter increment models are presented for 20 species/species groups. Selection of explanatory variables was done using a combination of forward and backward selection methods. The explained variance ranged from 10% to 53% depending on the species. Variables related to forest structure (basal area of the stand and relative size of the tree) contributed most to the explained variance, but environmental variables were important to account for spatial patterns. The type of environmental variables included differed greatly among species. Conclusions: The presented diameter increment models are the first of their kind that are applicable at the European scale. This is an important step towards the development of a new generation of forest development simulators that can be applied at the European scale, but that are sensitive to variations in growing conditions and applicable to a wider range of management systems than before. This allows European scale but detailed analyses concerning topics like CO2 sequestration, wood mobilisation, long term impact of management, etc.
Authors
Donghai Wu Philippe Ciais Nicolas Viovy Alan K. Knapp Kevin Wilcox Michael Bahn Melinda D. Smith Sara Vicca Simone Fatichi Jakob Zscheischler Yue He Xiangyi Li Akihito Ito Almuth Arneth Anna Harper Anna Ukkola Athanasios Paschalis Benjamin Poulter Changhui Peng Daniel Ricciuto David Reinthaler Guangsheng Chen Hanqin Tian Helene Genet Jiafu Mao Johannes Ingrisch Julia E.S.M. Nabel Julia Pongratz Lena R. Boysen Markus Kautz Michael Schmitt Patrick Meir Qiuan Zhu Roland Hasibeder Sebastian Sippel Shree R.S. Dangal Stephen Sitch Xiaoying Shi Yingping Wang Yiqi Luo Yongwen Liu Shilong PiaoAbstract
Field measurements of aboveground net primary productivity (ANPP) in temperate grasslands suggest that both positive and negative asymmetric responses to changes in precipitation (P) may occur. Under normal range of precipitation variability, wet years typically result in ANPP gains being larger than ANPP declines in dry years (positive asymmetry), whereas increases in ANPP are lower in magnitude in extreme wet years compared to reductions during extreme drought (negative asymmetry). Whether the current generation of ecosystem models with a coupled carbon– water system in grasslands are capable of simulating these asymmetric ANPP responses is an unresolved question. In this study, we evaluated the simulated responses of temperate grassland primary productivity to scenarios of altered precipitation with 14 ecosystem models at three sites: Shortgrass steppe (SGS), Konza Prairie (KNZ) and Stubai Valley meadow (STU), spanning a rainfall gradient from dry to moist. We found that (1) the spatial slopes derived from modeled primary productivity and precipitation across sites were steeper than the temporal slopes obtained from interannual variations, which was consistent with empirical data; (2) the asymmetry of the responses of modeled primary productivity under normal inter-annual precipitation variability differed among models, and the mean of the model ensemble suggested a negative asymmetry across the three sites, which was contrary to empirical evidence based on filed observations; (3) the mean sensitivity of modeled productivity to rainfall suggested greater negative response with reduced precipitation than positive response to an increased precipitation under extreme conditions at the three sites; and (4) gross primary productivity (GPP), net primary productivity (NPP), aboveground NPP (ANPP) and belowground NPP (BNPP) all showed concave-down nonlinear responses to altered precipitation in all the models, but with different curvatures and mean values. Our results indicated that most models overestimate the negative drought effects and/or underestimate the positive effects of increased precipitation on primary productivity under normal climate conditions, highlighting the need for improving eco-hydrological processes in those models in the future.
Authors
Daine Hernández-Ochandia Mayra G. Rodríguez Hernández Ricardo HolgadoAbstract
El frijol común (Phaseolus vulgaris L.) constituye un valioso alimento para la población de Latinoamérica; entre las numerosas plagas que lo afectan en la región están los nematodos parásitos de plantas (NPP) o fitonematodos. Los objetivos de este trabajo fueron analizar y resumir la información disponible acerca de las relaciones nematodos parásitos de plantas (NPP) - P. vulgaris en América Latina, profundizando en los avances, problemas y perspectivas del tema en Cuba, como un aporte a la preparación del personal vinculado a la producción y al manejo de nematodos en los frijoles que se producen para ser consumidos como grano seco. Se analizó la información contenida en las bases de datos SciELO, Sciencedirect y otros repositorios, donde se constató que en la región parasitan el frijol Meloidogyne arenaria (Neal) Chitwood, Meloidogyne decalineata Whitehead, Meloidogyne ethiopica Whitehead (syn. jun. Meloidogyne brasiliensis), Meloidogyne hapla Chitwood, Meloidogyne incognita (Kofoid y White) Chitwood, Meloidogyne inornata Lordello, Meloidogyne javanica (Treub) Chitwood, Meloidogyne luci Carneiro et al., Meloidogyne phaseoli Charchar et al. y Meloidogyne spp., Aphelenchoides besseyi Christie, Nacobbus aberrans (Thorne) Thorne y Allen y Pratylenchus spp. En los estudios recibieron la mayor atención los aspectos relacionados con la evaluación de cultivares y otras tácticas como la preparación del suelo, el intercalamiento y el uso de agentes biológicos. La información analizada indicó que más de 45 genotipos de frijol mostraron algún nivel de resistencia a Meloidogyne spp. (nematodos agalleros), sobresaliendo el cultivar Aporé en estudios en Brasil y Triunfo-70 en Cuba, lo que revela la existencia de materiales promisorios para programas de mejoramiento del frijol que se ejecutan en esos países. La importancia estratégica de este cultivo para Cuba, considerado como prioritario para la seguridad alimentaria, impone la necesidad de profundizar en los estudios de la interacción NPP-P. vulgaris, como elemento básico para el adecuado manejo del cultivo.
Authors
Carrie Joy Andrew Einar Heegaard Klaus Høiland Beatrice Senn-Irlet Thomas W. Kuyper Irmgard Krisai-Greilhuber Paul M. Kirk Jacob Heilmann-Clausen Alan C. Gange Simon Egli Claus Bässler Ulf Büntgen Lynne Boddy Håvard KauserudAbstract
Here we assess the impact of geographically dependent (latitude, longitude, and altitude) changes in bioclimatic (temperature, precipitation, and primary productivity) variability on fungal fruiting phenology across Europe. Two main nutritional guilds of fungi, saprotrophic and ectomycorrhizal, were further separated into spring and autumn fruiters. We used a path analysis to investigate how biogeographic patterns in fungal fruiting phenology coincided with seasonal changes in climate and primary production. Across central to northern Europe, mean fruiting varied by approximately 25 d, primarily with latitude. Altitude affected fruiting by up to 30 d, with spring delays and autumnal accelerations. Fruiting was as much explained by the effects of bioclimatic variability as by their large‐scale spatial patterns. Temperature drove fruiting of autumnal ectomycorrhizal and saprotrophic groups as well as spring saprotrophic groups, while primary production and precipitation were major drivers for spring‐fruiting ectomycorrhizal fungi. Species‐specific phenology predictors were not stable, instead deviating from the overall mean. There is significant likelihood that further climatic change, especially in temperature, will impact fungal phenology patterns at large spatial scales. The ecological implications are diverse, potentially affecting food webs (asynchrony), nutrient cycling and the timing of nutrient availability in ecosystems.
Abstract
The Baltic region includes in this report Fennoscandia (Norway, Sweden, Finland, Denmark), the Baltic states (Estonia, Latvia, Lithuania) and Poland. This region is fairly heterogeneous as regards forest history, forest policy, forest economy as well as climate and conditions for forest growth. The climate of the Baltic region is cool, but still drastically modified by the Gulfstream which skirts the western coast of Scandinavia, giving rise to much warmer summers and milder winters than expected based on the latitude. The warming associated with climate change is expected to be particularly pronounced in winter and at high latitudes. In coastal areas precipitation may increase notably. With elevated temperature, the frequency of both spring frost and drought events is predicted to increase in continental parts. The vegetation and forest types are heterogeneous. Fennoscandia has a large proportion of boreal vegetation where coniferous forests dominate and many broadleaves common in Central Europe are rare and scattered. In the Baltic region the most distinct marginal populations are those at the northern fringe of their distribution. The distribution ranges are limited by a combination of different factors such as low winter temperatures, short growing season either for growth or for seed maturation, soil types and human influence. Fragmentation may limit gene flow between stands, and some populations also show slight inbreeding. The countries in the region have protected jointly 4,9 M ha in the main MCPFE categories. The northern part of the region seems to put more weight on nature conservation through no intervention whereas the southern part emphasizes conservation through active management. The countries of the Baltic region have uploaded altogether 1'172 in situ genetic conservation units in the European Information System on Forest Genetic Resources (EUFGIS).
Authors
Thomas Dirnböck Gisela Pröll Kari Austnes Jelena Beloica Burkhard Beudert Roberto Canullo Alessandra De Marco Maria Francesca Fornasier Martyn Futter Klaus Goergen Ulf Grandin Maria Holmberg Antti-Jussi Lindroos Michael Mirtl Johan Neirynck Tomasz Pecka Tiina Maileena Nieminen Jørn-Frode Nordbakken Maximilian Posch Gert-Jan Reinds Edwin C. Rowe Maija Salemaa Thomas Scheuschner Franz Starlinger Aldona Katarzyna Uzieblo Salar Valinia James Weldon Wieger G W Wamelink Martin ForsiusAbstract
Atmospheric nitrogen (N) pollution is considered responsible for a substantial decline in plant species richness and for altered community structures in terrestrial habitats worldwide. Nitrogen affects habitats through direct toxicity, soil acidification, and in particular by favoring fast-growing species. Pressure from N pollution is decreasing in some areas. In Europe (EU28), overall emissions of NO x declined by more than 50% while NH3 declined by less than 30% between the years 1990 and 2015, and further decreases may be achieved. The timescale over which these improvements will affect ecosystems is uncertain. Here we use 23 European forest research sites with high quality long-term data on deposition, climate, soil recovery, and understory vegetation to assess benefits of currently legislated N deposition reductions in forest understory vegetation. A dynamic soil model coupled to a statistical plant species niche model was applied with site-based climate and deposition. We use indicators of N deposition and climate warming effects such as the change in the occurrence of oligophilic, acidophilic, and cold-tolerant plant species to compare the present with projections for 2030 and 2050. The decrease in N deposition under current legislation emission (CLE) reduction targets until 2030 is not expected to result in a release from eutrophication. Albeit the model predictions show considerable uncertainty when compared with observations, they indicate that oligophilic forest understory plant species will further decrease. This result is partially due to confounding processes related to climate effects and to major decreases in sulphur deposition and consequent recovery from soil acidification, but shows that decreases in N deposition under CLE will most likely be insufficient to allow recovery from eutrophication.
Authors
Shota Masumoto Motoaki Tojo Satoshi Imura Maria-Luz Herrero Masaki UchidaAbstract
The parasitic fungus Rhytisma polare is a common parasite on leaves of the polar willow (Salix polaris) in the high-Arctic polar semi-desert of Spitsbergen, Norway. Because Rhytisma spp. generally requires saturation with free water to develop ascospores, it is unclear how R. polare has ecologically adapted to the Arctic desert, where such water is very limited. In this study, the response of R. polare to diferent water conditions on Spitsbergen was investigated during the summer months of June–August in 2012. Field and laboratory experiments demonstrated that free water availability from rainfall or snowmelt is essential to facilitate ascostromal maturation and ascospore dispersal in R. polare. The feld experiments also revealed that the dispersal of ascospores produced on fallen leaves did not extend beyond a few meters. These results suggest that the free water requirement combined with the short spore-dispersal distance constrains the local occurrence of R. polare in the Arctic desert to locations where free water from rainfall and snowmelt is present.
Authors
Florencia A. Yannelli Gerhard Karrer Rea Hall Johannes Kollmann Tina HegerAbstract
Question Disturbed areas offer great opportunities for restoring native biodiversity, but they are also prone to invasion by alien plants. Following the limiting similarity hypothesis, we address the question of whether or not similarity of plant functional traits helps developing seed mixtures of native communities with high resistance to invasive species at an early stage of restoration. Location Centre of Greenhouses and Laboratories Dürnast, Technische Universität München, Freising, Germany. Methods Using a system of linear equations, we designed native communities maximizing the similarity between the native and two invasive species according to ten functional traits. We used native grassland plants, two invasive alien species that are often problematic in disturbed areas (i.e., Ambrosia artemisiifolia and Solidago gigantea) and trait information obtained from databases. The two communities were then tested for resistance against establishment of the two invaders separately in a greenhouse experiment. We measured height of the invasive species and above‐ground biomass, along with leaf area index, 4 and 8 months after sowing respectively. Results Both invasive species were successfully reduced by the native community designed to suppress S. gigantea dominated by small‐seeded species. These results could be considered as partial support for the limiting similarity hypothesis. However, given the success of this mixture against both invasive species, suppression was better explained by a seed density effect resulting from the smaller seed mass of the native species included in this mixture. Further, the dominance of a fast‐developing competitive species could also contribute to its success. Conclusions There was no unequivocal support for the limiting similarity hypothesis in terms of the traits selected. Instead we found that increasing seeding density of native species and selecting species with a fast vegetative development is an effective way to suppress invasive plants during early stages of restoration. If limiting similarity is used to design communities for restoration, early life‐history traits should be taken into account.
Authors
Marion Lang Julia Prestele Klaus Wiesinger Johannes Kollmann Harald AlbrechtAbstract
Diversity of arable plants in Europe has markedly declined during the past decades and many species have become threatened. Low‐intensity farming can offer potential retreats for these species, while spontaneous dispersal between such fields is unlikely. Thus, reintroduction of endangered species is necessary to restore agrobiodiversity. To test the applicability under real farm conditions, we seeded a mixture of three winter annuals (Legousia speculum‐veneris, Consolida regalis, Lithospermum arvense) at 850 seeds/m2 on four organic farms near Munich, Germany, in autumn 2011. Seed production and soil seed banks were investigated on four plots within one field on each farm for 3 years. In addition, we evaluated seed dispersal caused by arable management along the main machining direction. In the first year, winter cereals were cultivated and the study species emerged at all sites with a seed production mostly exceeding the initial sowing rates. In the third year, species establishment varied depending on crop rotations. Seed production of L. speculum‐veneris was higher than in the two other species, and exceeded the number of originally sown seeds up to 20 times. While L. speculum‐veneris became very common in the seed bank, C. regalis was less abundant and L. arvense hardly developed a soil seed bank. Seeds of L. speculum‐veneris and L. arvense were found up to 15 m and seeds of C. regalis up to 13 m away from the sown plots. We suggest using seed production, seed bank, and dispersal as key indicators to evaluate establishment of reintroduced arable plants.
Authors
Caroline Brophy John A. Finn Andreas Lüscher M. Suter Laura Kirwan Maria-Teresa Sebastià Aslaug Helgadottir Ole Hans Baadshaug G. Bélanger Alistair Black Rosemary P. Collins Jure Čop Sigridur Dalmannsdottir Ignacio Delgado Anjo Elgersma Mick Fothergill Bodil E. Frankow-Lindberg An Ghesquiere Barbara Golinska Piotr Golinski Philippe Grieu Anne-Maj Gustavsson Mats Höglind Olivier Huguenin-Elie Marit Jørgensen Zydre Kadziuliene Päivi Kurki Rosa Llurba Tor Lunnan Claudio Porqueddu Ulrich Thumm John ConnollyAbstract
Increasing species diversity often promotes ecosystem functions in grasslands, but sward diversity may be reduced over time through competitive interactions among species. We investigated the development of species’ relative abundances in intensively managed agricultural grassland mixtures over three years to identify the drivers of diversity change. A continental-scale field experiment was conducted at 31 sites using 11 different four-species mixtures each sown at two seed abundances. The four species consisted of two grasses and two legumes, of which one was fast establishing and the other temporally persistent. We modelled the dynamics of the four-species mixtures over the three-year period. The relative abundances shifted substantially over time; in particular, the relative abundance of legumes declined over time but stayed above 15% in year three at many sites. We found that species’ dynamics were primarily driven by differences in the relative growth rates of competing species and secondarily by density dependence and climate. Alongside this, positive diversity effects in yield were found in all years at many sites.
Abstract
Knowledge about the botanical composition of grassland for silage is important regarding composition of seed mixtures, control of weeds, choice of harvest times and feeding strategies. The botanical composition of 185 fields in the mountain regions of southern Norway was examined using the dryweight rank method. The survey shows that the youngest grasslands (age 1 - 3 years) were dominated by the sown species with Phleum pratense L. the species with the highest proportion in the sward. In 4 - 6 year old grasslands, the proportion of sown species was reduced with the exception of Poa pratensis L., and Elytrigia repens L. had the highest proportion of unsown species. The proportion of Festuca pratensis (Huds.) was reduced at the same rate as Phleum pratense L. In grasslands of greater age (> 6 years) Poa pratensis L. and Elytrigia repens L. had the highest occurrence. The content of herbs increased with age, and Ranunculus repens L. and Taraxacum officinale F.H. Wigg were the most frequent species. The average clover content was < 6% of DM yield. The impact of Elytrigia repens L. on forage yield and quality should be further examined due to the high occurrence. Poa pratensis L. or other long-lasting grass species should be included in seed mixtures for this region when the grassland is intended to last more than three years.
Authors
Sabine Rosner Notburga Gierlinger Matthias Klepsch Bo Karlsson Rob Evans Sven-Olof Lundqvist Jan Svetlik Isabella Børja Lise Dalsgaard Kjell Andreassen Svein Solberg Steven JansenAbstract
Projected climate change scenarios such as frequently occurring dry summer spells are an enormous threat to the health of boreal conifer forests. We identified visible features indicating wood with tracheids predisposed for hydraulic and mechanical dysfunction in Norway spruce, suggest why this is formed during severe summer drought and hypothesised on mechanism that would cause tracheid collapse and stem cracks. Trees from southern Sweden that showed signs of severe reaction to drought, i.e. stem cracks along the trunk, were compared to healthy, undamaged trees. Rings investigated included those formed in 2006, a year with an extremely dry summer season in the study region. In southern Norway, we investigated trees with and without drought-induced top dieback symptoms. We analysed anatomical features such as tracheid lumen diameter, thickness of cell wall and its various layers (S1, S2 and S3), applied Raman imaging in order to get information on the lignin distribution in the cell wall and the compound middle lamellae and performed hydraulic flow and shrinkage experiments. Although tracheids in annual rings with signs of collapse had higher tangential lumen diameters than those in “normal” annual rings, we conclude that collapse of tracheid walls depends mainly on wall thickness, which is genetically determined to a large extent. Spruce trees that produce earlywood with extremely thin cell walls can develop wall collapse and internal cracks under the impact of dry spells. We also present a new diagnostic tool for detecting individuals that are prone to cell wall collapse and stem cracks: Lucid bands, i.e. bands in the fresh sapwood with very thin cell walls and inhomogeneous lignin distribution in the S-layers and the compound middle lamellae that lost their hydraulic function due to periods of severe summer drought. The detection of genotypes with lucid bands could be useful for an early selection against individuals that are prone to stem cracks under the impact of severe summer drought, and also for early downgrading of logs prone to cracking during industrial kiln drying
Abstract
In order to establish the relationship between spectral reflectance and grass yield, we used a UAV-based hyperspectral camera and ground-based spectroradiometry to image a number of cultivated grasslands of different age and productivity in northern Norway. In addition, samples were taken to determine biomass and grass species composition. We investigated a number of vegetation indices as well as regression analysis to identify which spectral reflectance features can be used to map crop yield. We found poor relationships between NDVI and yield, but were able to obtain an acceptable relationship using all 15 available bands in the visible-near infrared range. Bands in the near infrared appear to contain most of the information related to yield.
Authors
Mark McMullan Maryam Rafiqi Gemy Kaithakottil Bernardo J. Clavijo Lorelei Bilham Elizabeth Orton Lawrence Percival-Alwyn Ben J. Ward Anne Edwards Diane G.O. Saunders Gonzalo Garcia Accinelli Jonathan Wright Walter Verweij Georgios Koutsovoulos Kentaro Yoshida Tsuyoshi Hosoya Louisa Williamson Philip Jennings Renaud Ioos Claude Husson Ari Hietala Adam Vivian-Smith Halvor Solheim Dan MaClean Christine Fosker Neil Hall James K.M. Brown David Swarbreck Mark Blaxter J. Allan Downie Matthew D. ClarkAbstract
Accelerating international trade and climate change make pathogen spread an increasing concern. Hymenoscyphus fraxineus, the causal agent of ash dieback, is a fungal pathogen that has been moving across continents and hosts from Asian to European ash. Most European common ash trees (Fraxinus excelsior) are highly susceptible to H.fraxineus, although a minority (~5%) have partial resistance to dieback. Here, we assemble and annotate a H.fraxineus draft genome, which approaches chromosome scale. Pathogen genetic diversity across Europe and in Japan, reveals a strong bottleneck in Europe, though a signal of adaptive diversity remains in key host interaction genes. We find that the European population was founded by two divergent haploid individuals. Divergence between these haplotypes represents the ancestral polymorphism within a large source population. Subsequent introduction from this source would greatly increase adaptive potential of the pathogen. Thus, further introgression of H.fraxineus into Europe represents a potential threat and Europe-wide biological security measures are needed to manage this disease.
Authors
Gudrun Schwilch Tatenda Lemann Örjan Berglund Carlo Camarotto Artemi Cerdà Ioannis N. Daliakopoulos Silvia Kohnová Dominika Krzeminska Teodoro Maranon René Rietra Grzegorz Siebielec Johann Thorsson Mark Tibbett Sandra Valente Hedwig van Delden Jan van den Akker Simone Verzandvoort Nicoleta Olimpia Vrînceanu Christos Zoumides Rudi HesselAbstract
Only a few studies have quantified and measured ecosystem services (ES) specifically related to soil. To address this gap, we have developed and applied a methodology to assess changes in ecosystem services, based on measured or estimated soil property changes that were stimulated by soil management measures (e.g., mulching, terracing, no-till). We applied the ES assessment methodology in 16 case study sites across Europe representing a high diversity of soil threats and land use systems. Various prevention and remediation measures were trialled, and the changes in manageable soil and other natural capital properties were measured and quantified. An Excel tool facilitated data collection, calculation of changes in ecosystem services, and visualization of measured short-term changes and estimated long-term changes at plot level and for the wider area. With this methodology, we were able to successfully collect and compare data on the impact of land management on 15 different ecosystem services from 26 different measures. Overall, the results are positive in terms of the impacts of the trialled measures on ecosystem services, with 18 out of 26 measures having no decrease in any service at the plot level. Although methodological challenges remain, the ES assessment was shown to be a comprehensive evaluation of the impacts of the trialled measures, and also served as an input to a stakeholder valuation of ecosystem services at local and sub-national levels.
Authors
Panu Korhonen Taru Palosuo Tomas Persson Mats Höglind Guillaume Jégo Marcel Van Oijen Anne-Maj Gustavsson Gilles Bélanger Perttu VirkajärviAbstract
During the past few years, several studies have compared the performance of crop simulation models to assess the uncertainties in model-based climate change impact assessments and other modelling studies. Many of these studies have concentrated on cereal crops, while fewer model comparisons have been conducted for grasses. We compared the predictions for timothy grass (Phleum pratense L.) yields for first and second cuts along with the dynamics of above-ground biomass for the grass simulation models BASGRA and CATIMO, and the soil-crop model STICS. The models were calibrated and evaluated using field data from seven sites across Northern Europe and Canada with different climates, soil conditions and management practices. Altogether the models were compared using data on timothy grass from 33 combinations of sites, cultivars and management regimes. Model performances with two calibration approaches, cultivar-specific and generic calibrations, were compared. All the models studied estimated the dynamics of above-ground biomass and the leaf area index satisfactorily, but tended to underestimate the first cut yield. Cultivar-specific calibration resulted in more accurate first cut yield predictions than the generic calibration achieving root mean square errors approximately one third lower for the cultivar-specific calibration. For the second cut, the difference between the calibration methods was small. The results indicate that detailed soil process descriptions improved the overall model performance and the model responses to management, such as nitrogen applications. The results also suggest that taking the genetic variability into account between cultivars of timothy grass also improves the yield estimates. Calibrations using both spring and summer growth data simultaneously revealed that processes determining the growth in these two periods require further attention in model development.
Abstract
No abstract has been registered
Authors
Leonardo Ciaccheri Lorenza Tuccio Andrea A. Mencaglia Anna G. Mignani Ewelina Hallmann Kalina Sikorska-Zimny Stanislaw Kaniszewski Michel Verheul Giovanni AgatiAbstract
Non-destructive tools for evaluating the lycopene content in tomatoes are of great interest to the entire fruit chain because of an increasing demand for beneficial health products. With the aim of developing compact low-cost reflectance sensors for lycopene determination, we compared Partial Least Squares (PLS) prediction models by using either directional or total reflectance in the 500–750 nm range. Directional reflectance at 45° with respect to the LED lighting direction was acquired by means of a compact spectrometer sensor. Total reflectance was acquired through a 50-mm integrating sphere connected to a spectrometer. The analysis was conducted on two hydroponic greenhouse cultivated red tomato varieties, namely the large round ‘Dometica’ (average diameter: 57 mm) and the small cherry ‘Juanita’ (average diameter: 26 mm). For both varieties, the spectral variance of directional reflectance was well correlated to that of total reflectance. The performances of the PLS prediction models were also similar, with R2 of cross-validation between 0.73 and 0.81. The prediction error, relative to the mean lycopene content of full ripe tomatoes, was similar: i.e. around 16–17% for both varieties and sensors. Our results showed that directional reflectance measured by means of portable, low-cost and compact LED-based sensors can be used with an adequate precision for the non-destructive assessment of lycopene in tomatoes.
Abstract
Climate change could increase fire risk across most of the managed boreal forest. Decreasing this risk by increasing the proportion of broad-leaved tree species is an overlooked mitigation–adaption strategy with multiple benefits.
Authors
Krishna Reddy Kakumanu Yella Reddy Kaluvai M. Balasubramanian Sekhar Udaya Nagothu Gurava Reddy Kotapati Sunitha KaranamAbstract
Climate change adversely affects the determinants of agriculture. Adaptation serves as an important strategy to reduce the adverse effects of climate change (variability) and vulnerability of the people. Adaptation through an innovation programme was implemented for 4 years during 2012–2016 to improve the adaptive capacity in agriculture and the water sectors through capacity building and implementation in the Krishna River Basin, India. Primary data were collected from 178 farm households of the Nagarjuna Sagar Project command area covering both adopters and non‐adopters of water‐saving interventions from the study area. The double difference method was used to analyse the impact of adaptation through capacity building and implementation. The water‐saving interventions include alternate wetting and drying (AWD) in rice, a modified system of rice intensification (MSRI) and direct seeding of rice (DSR). The capacity building and water saving increased crop yields by 0.96, 0.93 and 0.77 t ha−1 through AWD, MSRI and DSR respectively. The three practices have increased farmers’ income and decreased the cost of cultivation in DSR by Rs.11 000 (US$169) ha−1. The methods can be more focused in canal commands on a larger scale for equal distribution of water to all the head, middle and tail‐end regions.
Abstract
Purpose: Due to environmental concerns, efforts are made to replace the use of peat in horticultural growth media by organic wastes. Four growth media were prepared with the purpose of achieving adequate physical and chemical properties for plant production. Materials and methods: Growth media prepared from mixtures of coir (C) and paper sludge (P), respectively, with two biogas digestates from food waste (D1 and D2), were tested. These mixtures, 20% D1 or D2 + 80% C or P (v/v), were evaluated as growth media for tomato (Solanum lycopersicum L.) and lettuce (Lactuca sativa L.). Results and conclusion: The growth media were all physically stable during the growing period, provided all the macronutrients and most of the micronutrients necessary for plant growth, adequate pH conditions, as well as an adequate electrical conductivity. The mixture of D2 and P produced the highest biomass compared to a mineral fertilised peat (control), with a biomass production of 76% of the control for lettuce and 54% for tomato. Causes for the biomass reduction relative to the control may be related to ammonium toxicity effects, and/or limited plant-available water. The digestates, particularly D1, seemed also to have a phytotoxic effect on the germination.
Abstract
The awareness of sediment and nutrient loss from non-point sources are of increasing environmental concern as measures to reduce point source inputs to surface waters have been introduced. Mitigation efforts to reduce loss of particles and nutrients from agriculture in Norway and other countries have mainly focused on surface runoff, whereas sub-surface drainage has received little attention. However, research has shown that the sub-surface field drains are transporting both sediment and nutrients rapidly to the watercourses. Despite these established facts there has been little development of measures to reduce these losses. This article describes how Lightweight Aggregates (LWA), Leca®, can mitigate some of the environmental challenges connected to sub-surface field drains. A field experimental project was performed to assess the effects on drainage water quality hydrological performance and functionality of drainage systems based on Lightweight Aggregates compared to traditional pipe drains. Registrations of the performance of the systems were done in two separate periods, 1992–1993 and 1999–2000. After 2000 no measurement programme has run. The functionality of the drainage systems was registered in connection to ordinary farming activity. In 1999–2000 LWA drains showed particularly good performance with regard to reducing the content of Phosphorus, 40–90 % reduction in Total-P. The drainage water from the LWA drains contained less than half the amount of suspended solids compared to traditional pipe drains. The results from 1993 showed no significant difference between LWA drains and pipe drains with respect to Nitrogen. The results from 1999/2000 showed higher loss of Nitrogen through pipe drains with no envelope compared to all other systems. LWA drains may be particularly useful in reducing particles and nutrient loads from cultivated flat drained areas adjacent to environmentally sensitive and ecologically important water ecosystems. Further investigations are recommended to optimise the design of LWA drains.
Authors
Krishna Reddy Kakumanu Yella Reddy Kaluvai Sekhar Udaya Nagothu Narayan Reddy Lati Gurava Reddy Kotapati Sunitha KaranamAbstract
Climate change characterized by global warming has become a hotspot of research in recent years for water resources, agriculture,ecology and other disciplines. In India, studies have shown an increasing trend in surface temperature, with decreasing trends inrainfall. Farmers are also more affected by the climate variability which has a serious influence on their production and income.The climate change and adaptation (ClimaAdapt) programme was implemented from 2012 to 2016 to build farm-level capacitiesand enhance the adaptive capacity of the agricultural and water sectors in the Krishna basin of Andhra Pradesh and Telanganastates. Water-saving interventions such as direct seeded rice, a modified system of rice intensification and alternate wetting anddrying (AWD) of rice were implemented in a cluster approach and enhanced water productivity. The training and implementationprogrammes increased the adaptation and awareness of farmers. Water measurements were carried out by usingflumes andultrasonic sensors. The area under direct seeded rice has increased to 64% in the study district and 77% of the trained farmersare adopting the practice. Capacity building, implementation and science–policy linkages are the key pillars of the programmeto improve the adaptive capacity and scaling-up of water management practices.
Abstract
Sewage sludge is a significant phosphorus resource that should be better utilized in the plant production than today. However, phosphorus in sludge produced after precipitation with aluminium and iron coagulants have low plant availability. This article presents results from plant growth experiments where plant available phosphorus in lime precipitated sewage sludge from Bokerøya and Skådevika wastewater treatment plants was studied. Both wastewater treatment plants normally use iron as coagulant aid, but Skådevika produced in addition a sludge to the experiments without using iron. The growth experiments included a pot experiment in greenhouse with three harvests of Italian ryegrass (Lolium multiflorum) and a two-year field experiment in cereals in Southeast Norway. The results showed that precipitation with lime gives a sludge with higher plant availability of phosphorus than what is earlier found for aluminium/ iron precipitated sludge. This was special the case with moderate use of iron as coagulant aid.
Authors
Xiao Huang Shaoqiang Ni Chaoqing Yu Jim Hall Conrad Zorn Xiaomeng HuangAbstract
Precipitation is an important source of soil water, which is critical to crop growth, and is therefore an important input when modelling crop growth. Although advances are continually being made in predicting and recording precipitation, input uncertainty of precipitation data is likely to influence the robustness of parameter estimate and thus the predictive accuracy in soil water and crop modelling. In this study, we use the Bayesian total error analysis (BATEA) method for the water-oriented crop model AquaCrop to identify the input uncertainty from multiple precipitation products respectively, including gauge-corrected grid dataset CPC, remote sensing based TRMM and reanalysis based ERA-Interim. This methodology uses latent variables to correct the input data errors. Adopting a single-multiplier method for precipitation correction, we simulate maize growth in both field and regional levels in China for a range of different possible climatic scenarios. Meanwhile, we use the average of multiple products for model driving in comparison. The results show that the BATEA method can consistently reduce uncertainty for crop growth prediction among different precipitation products. In regional simulation, the improvements for the three products are 1%, 7.3% and 2.8% on average in drought scenarios. These results imply the BATEA approach can be of great assistance for crop modeling studies and agricultural assessments under future changing climates.
Authors
Xiao Huang Chaoqing Yu Jiarui Fang Guorui Huang Shaoqiang Ni Jim Hall Conrad Zorn Xiaomeng Huang Wenyuan ZhangAbstract
Crop models are widely used to evaluate the response of crop growth to drought. However, over large geographic regions, the most advanced models are often restricted by available computing resource. This limits capacity to undertake uncertainty analysis and prohibits the use of models in real-time ensemble forecasting systems. This study addresses these concerns by presenting an integrated system for the dynamic prediction and assessment of agricultural yield using the top-ranked Sunway TaihuLight supercomputer platform. This system enables parallelization and acceleration for the existing AquaCrop, DNDC (DeNitrification and DeComposition) and SWAP (Soil Water Atmosphere Plant) models, thus facilitating multi-model ensemble and parameter optimization and subsequent drought risk analysis in multiple regions and at multiple scales. The high computing capability also opens up the possibility of real-time simulation during droughts, providing the basis for more effective drought management. Initial testing with varying core group numbers shows that computation time can be reduced by between 2.6 and 3.6 times. Based on the powerful computing capacity, a county-level model parameter optimization (2043 counties for 1996–2007) by Bayesian inference and multi-model ensemble using BMA (Bayesian Model Average) method were performed, demonstrating the enhancements in predictive accuracy that can be achieved. An application of this system is presented predicting the impacts of the drought of May–July 2017 on maize yield in North and Northeast China. The spatial variability in yield losses is presented demonstrating new capability to provide high resolution information with associated uncertainty estimates.
Authors
Trygve S. Aamlid Oiva Niemelainen Klaus Paaske David Widmark Pentti Ruuttunen Auli KedonperaAbstract
Greenkeepers are looking for alternatives to fungicides for control of turfgrass diseases. Our objective was to evaluate a petroleum- derived spray oil with a blue-green pigment for control of Microdochium patch/pink snow mold (Microdochium nivale) on golf course putting greens with various durations of snow cover. The spray oil was applied at rates 27 or 54 L ha–1 every third week from late August or September to December, either alone, in tank mixture with potassium phosphite (3 kg PO3 ha–1) or in tank mixture with half rate of fungicides approved for turf, in five 1-yr trials in the Nordic countries. The oil was as effective or more effective than fungicides and gave, on average, 94 and 98% disease control at rates 27 and 54 L ha–1, respectively. Tank mixtures with half rate of prochloraz + propioconazole and fludioxonil did not increase disease suppression in a trial with 79 d snow cover. Phosphite reduced disease severity in one trial only and did not improve disease control or turfgrass quality when tank-mixed with the oil. The pigment in the spray oil was highly persistent and improved turfgrass greenness except in a trial where the combination of oil and ice cover gave a transitory black color at ice melt. Another trial with long snow cover showed a drop in turfgrass quality in spring as the spray oil prevented normal green-up. In conclusion, this research shows that a spray oil has the potential to reduce fungicide use on Nordic golf courses.
Abstract
Carbonic anhydrase (CA) plays an important physiological role in all biological systems by accelerating the interconversion of CO2 and HCO3 −. In algae, CA is essential for photosynthesis: external CA (CAext) dehydrates HCO3 −, enhancing the supply of CO2 to the cell surface, and internal CA (CAint) interconverts HCO3 − and CO2 to maintain the inorganic carbon (Ci) pool and supply CO2 to RuBisCO. We frst conducted a literature review comparing the conditions in which CA extraction and measurement have been carried out, using the commonly used Wilbur–Anderson method. We found that the assay has been widely modifed since its introduction in 1948, mostly without being optimized for the species tested. Based on the review, an optimized protocol for measuring CA in Macrocystis pyrifera was developed, which showed that the assay conditions can strongly afect CA activity. Tris–HCl bufer gave the highest levels of CA activity, but phosphate bufer reduced activity signifcantly. Bufers containing polyvinylpyrrolidone (PVP) and dithiothreitol (DTT) stabilized CA. Using the optimized assay, CAext and CAint activities were readily measured in Macrocystis with higher precision compared to the non-optimized method. The CAint activity was 2×higher than CAext, which is attributed to the Ci uptake mechanisms of Macrocystis. This study suggests that the CA assay needs to be optimized for each species prior to experimental work to obtain both accurate and precise results.
Authors
Kaishan Song Zhidan Wen Yijun Xu Hong Yang Lili Lyu Ying Zhao Chong Fang Yingxin Shang Jia DuAbstract
Dissolved carbon in lakes play a vital role in the global carbon cycling. The concentration and dynamics of lake dissolved carbon can be influenced by both the surrounding landscape and a combination of physical, chemical and biological processes within the lakes themselves. From 2009 to 2016, we conducted a large-scale assessment of dissolved organic carbon (DOC) and dissolved inorganic carbon (DIC) in 249 lakes across a diverse range of climatic, geopedologic, topographical and hydrological conditions in five Chinese limnetic regions: the East Limnetic Region (ELR), the Northeast Limnetic Region (NLR), the Inner Mongolia-Xinjiang Limnetic Region (MXR), the Yungui Limnetic Region (YGR), and the Tibet-Qinghai Limnetic Region (TQR). We found that the density of the organic matter in the soil in the surrounding landscape plays an important role in the DOC and DIC in lake water, as was evidenced by the high DOC and DIC levels in the NLR, where the soil is respectively organically rich. Conditions in the arid and semi-arid environments (i.e. TQR and MXR) have created a number of brackish/saline lakes and here we found that, DOC and DIC levels (median: 21.79 and 93.72 mg/L, respectively) are significantly higher than those in the freshwater lakes (median: 5.80 and 29.38 mg/L). It also appears to be the case that the trophic state of freshwater lakes influences the spatial variation of DOC. This can be seen in the relationships between DOC and trophic state index (TSI) in agriculturally-dominated regions such as the ELR (R2 = 0.59, p < 0.01), NLR (R2 = 0.65, p < 0.001), and YGR (R2 = 0.78, p < 0.001). Additionally, a close relationship between DOC and DIC can be found in lake waters with different trophic states (eutrophic: slp = 0.63, R2= 0.69; mesotrophic: slp = 1.03, R2 = 0.65; oligotrophic: slp = 1.00, R2 = 0.64). This indicates that human activities influence the quantity and quality of dissolved carbon in inland water across China. This study is able to provide insights regarding the potential effects of climate change and changes in land-use upon the amount of dissolved carbon in lake water.
Authors
Stef Bokhorst Matty P. Berg Guro Kristine Edvinsen Jacintha Ellers Amber Heitman Laura Jaakola Hanne Mæhre Gareth K. Phoenix Hans Tømmervik Jarle W. BjerkeAbstract
Climate change is one of many ongoing human-induced environmental changes, but few studies consider interactive effects between multiple anthropogenic disturbances. In coastal sub-arctic heathland, we quantified the impact of a factorial design simulating extreme winter warming (WW) events (7 days at 6–7∘C) combined with episodic summer nitrogen (+N) depositions (5 kg N ha-1) on plant winter physiology, plant community composition and ecosystem CO2 fluxes of an Empetrum nigrum dominated heathland during 3 consecutive years in northern Norway. We expected that the +N would exacerbate any stress effects caused by the WW treatment. During WW events, ecosystem respiration doubled, leaf respiration declined (-58%), efficiency of Photosystem II (Fv/Fm) increased (between 26 and 88%), while cell membrane fatty acids showed strong compositional changes as a result of the warming and freezing. In particular, longer fatty acid chains increased as a result of WW events, and eicosadienoic acid (C20:2) was lower when plants were exposed to the combination of WW and +N. A larval outbreak of geometrid moths (Epirrita autumnata and Operophtera brumata) following the first WW led to a near-complete leaf defoliation of the dominant dwarf shrubs E. nigrum (-87%) and Vaccinium myrtillus (-81%) across all experimental plots. Leaf emergence timing, plant biomass or composition, NDVI and growing season ecosystem CO2 fluxes were unresponsive to the WW and +N treatments. The limited plant community response reflected the relative mild winter freezing temperatures (-6.6∘C to -11.8∘C) recorded after the WW events, and that the grazing pressure probably overshadowed any potential treatment effects. The grazing pressure and WW both induce damage to the evergreen shrubs and their combination should therefore be even stronger. In addition, +N could have exacerbated the impact of both extreme events, but the ecosystem responses did not support this. Therefore, our results indicate that these sub-arctic Empetrum-dominated ecosystems are highly resilient and that their responses may be limited to the event with the strongest impact.
Authors
David Kopecký Joost Baert Susanne Barth J Bartos Vladimir Cernoch J. Dolezel Dermot Grogan John Harper M. Humphreys T. Ksiazczyk Liv Østrem E. Paszkowski Dejan Sokolovič Zbigniew Zwierzykowski Marc GhesquièreAbstract
A comprehensive set of Festulolium cultivars from on-going field trials in the Eucarpia network was characterised at the chromosome level using genomic in situ hybridization (GISH) and by Diversity Array Technology (DArT) markers. Both technologies were found to be complementary in describing the breeding history of the plant material. The genomic composition of the Lolium X Festuca cultivars varied from those that comprised equivalent proportions of their parental genomes to introgression lines where small chromosome segments of Festuca had been translocated onto Lolium chromosomes. The breadth of genotype combinations found within the grass cultivars described represents an important resource of genetic variations necessary to combat the diverse abiotic stresses encountered within Europe, including safeguards against prolonged exposure to harsh weather conditions. It is likely that in future plant breeding, genotyping will contribute to precision-transfers of targeted Festuca genes into Lolium germplasm in order to enhance resilience to climate change.
Authors
Liv Østrem Torben Asp Marc Ghesquière Yoshinori Sanada Odd Arne RognliAbstract
Norwegian cultivars and breeding materials of perennial ryegrass and Festulolium were planted at three locations in Denmark, France and Japan for test-ing of resistance against leaf diseases. In general, all plant materials were susceptible to crown rust. The highest incidence of rust attack occurred at the French site, which due to its climatic conditions might be the most suitable testing site for future scoring of similar plant material. Entries based on introgressed genetic materials from UK were most resistant towards crown rust. Crown rust resistance needs increased focus as a breeding objective in the Nordic region due to climate changes, which will most likely lead to increased infection of leaf diseases.
Abstract
We examined the influence of fertigation on vegetative and generative parameters of strawberry plants (Fragaria × ananassa Duch.) and evaluated rapid analysing tools for N and K in leaf tissue. The experiments were undertaken in an open polytunnel on “table top” with ‘Sonata’ and ‘Korona’ grown in 2-L pots filled with a peat-based soil mixture. The experimental design was a randomized plot with three replications. Plants were fertigated with EC levels of 0.5, 1.0, 1.5 and 2.0 mS cm-1, based on two stock solutions of 7.5 kg YaraLiva™ Calcinit and 7.5 kg Kristalon™ Indigo, both dissolved in 100 L of water. Percentage N and K in leaves differed between analysing methods, cultivars, EC and date. We found interactions between the cultivar and EC level and between date and cultivar for N and K in leaf. Analysing NO3- by a photometric method (PM) in a lab, and by Laqua twin (LT), showed significant interaction with N% of leaf dry matter (DM) only for LT (r2=0.36). N% increased with higher EC level, more for ‘Korona’ than for ‘Sonata’. LT K+ did not correlate with K% (r2=0.014). The number of crowns and runners increased for both cultivars up to EC 1.5, while the number of leaves was unaffected. Petioles were the shortest at the lowest EC. Flower initiation was earlier at low EC in both cultivars. In the following spring, the time to flowering and first harvest was reduced with the decreasing EC. The number of flowers per plant increased up to EC 1.5, but dropped strongly at EC 2.0 for ‘Korona’, while ‘Sonata’ had a gradual increase of flowers with the increasing EC, but the number was only a third of ‘Korona’, except at EC 2.0, where the amount was equal for both cultivars. The conclusion can be drawn that LT correlated better than ChlDualex with N in strawberry leaves. However, r2 was only 0.36 indicating that LT NO3- is a coarse management tool. LT K+ was not a promising tool for rapid K+ test in these experiments. ‘Korona’ seemed to benefit of higher N levels for both vegetative growth and generative development than ‘Sonata’ up to EC 1.5, but ‘Sonata’ reached a higher floral primordia development stage in early October.
Authors
Liviu Nichiforel Kevin Keary Philippe Deuffic Gerhard Weiss Bo Jellesmark Thorsen Georg Winkel Mersudin Avdibegović Zuzana Dobsinska Diana Feliciano Paola Gatto Elena Górriz Mifsud Marjanke Hoogstra-Klein Michal Hrib Teppo Hujala Laszlo Jager Vilém Jarský Krzysztof Jodlowski Anna Lawrence Diana Lukmine Špela Pezdevšek Malovrh Jelena Nedeljkovic Dragan Nonic Silvija Krajter Ostoic Klaus Pukall Jacques Rondeux Theano Samara Zuzana Sarvašová Ramona Elena Scriban Rita Šilingienė Milan Sinko Makedonka Stojanovska Vladimir Stojanovski Nickola Stoyanov Meelis Teder Birger Vennesland Lelde Vilkriste Erik Wilhelmsson Jerylee Wilkes-Allemann Laura BouriaudAbstract
Private forests are widespread in Europe providing a range of ecosystem services of significant value to society, and there are calls for novel policies to enhance their provision and to face the challenges of environmental changes. Such policies need to acknowledge the importance of private forests, and importantly they need to be based on a deep understanding of how property rights held by private forest owners vary across Europe. We collected and analysed data on the content of property rights based on formal legal requirements existing in 31 European jurisdictions. To allow a comparison across jurisdictions, we constructed an original Property Rights Index for Forestry encompassing five rights domains (access, withdrawal, management, exclusion and alienation). We documented substantial variation of the private forest owners’ rights, and notably to i) make decisions in operational management and the formulation of management goals, ii) withdraw timber resources from their forest, and iii) exclude others from the use of forest resources. We identified broad relations between the scope for decision making of private forest owners and jurisdictions’ former socio-political background and geographical distribution. The variation in the content of property rights has implications for the implementation of international environmental policies, and stresses the need for tailored policy instruments, when addressing European society’s rural development, the bioeconomy, climate change mitigation measures and nature protection strategies.
Authors
Roland Bol Gerard Gruau Per-Erik Mellander Rémi Dupas Marianne Bechmann Eva Skarbøvik Magdalena Bieroza Faruk Djodjic Miriam Glendell Philip Jordan Bas van der Grift Michael Rode Erik Smolders Mieke Verbeeck Sen Gu Erwin Klumpp Ina Pohle Maelle Fresne Chantal Gascuel-OdouxAbstract
In this paper, we outline several recent insights for the priorities and challenges for future research for reducing phosphorus (P) based water eutrophication in the agricultural landscapes of Northwest Europe.We highlight that new research efforts best be focused on headwater catchments as they are a key influence on the initial chemistry of the larger river catchments, and here many management interventions are most effectively made. We emphasize the lack of understanding on how climate change will impact on P losses from agricultural landscapes. Particularly, the capability to disentangle current and future trends in P fluxes, due to climate change itself, from climate driven changes in agricultural management practices and P inputs. Knowing that, future climatic change trajectories for Western Europe will accelerate the release of the most bioavailable soil P. We stress the ambiguities created by the large varieties of sources and storage/transfer processes involved in P emissions in landscapes and the need to develop specific data treatment methods or tracers able to circumvent them, thereby helping catchment managers to identify the ultimate P sources that most contribute to diffuse P emissions. We point out that soil and aqueous P exist not only in various chemical forms, but also in range of less considered physical forms e.g., dissolved, nanoparticulate, colloidal and other particulates, all affected differently by climate as well as other environmental factors, and require bespoke mitigation measures. We support increased high resolution monitoring of headwater catchments, to not only help verify the effectiveness of catchments mitigation strategies, but also add data to further develop new water quality models (e.g., those include Fe-P interactions) which can deal with climate and land use change effects within an uncertainty framework. We finally conclude that there is a crucial need for more integrative research efforts to deal with our incomplete understanding of the mechanisms and processes associated with the identification of critical source areas, P mobilization, delivery and biogeochemical processing, as otherwise even highintensity and high-resolution research efforts will only reveal an incomplete picture of the full global impact of the terrestrial derived P on downstream aquatic and marine ecosystems.
Authors
Kristi Parro Kajar Köster Kalev Jogiste Katrin Seglinš Allan Sims John A. Stanturf Marek MetslaidAbstract
Boreal forests are an important carbon (C) sink and fire is the main natural disturbance, directly affecting the Ccycle via emissions from combustion of biomass and organic matter and indirectly through long-term changes in C-dynamics including soil respiration. Carbon dioxide (CO2) emission from soil (soil respiration) is one of the largest fluxes in the global C-cycle. Recovery of vegetation, organic matter and soil respiration may be influenced by the intensity of post-fire management such as salvage logging. To study the impact of forest fire, fire and salvage, and recovery time on soil respiration and soil C and N content, we sampled two permanent research areas in north-western Estonia that were damaged by fire: Vihterpalu (59°13′ N 23°49′ E) in 1992 and Nõva (59°10′ N 23°45′ E) in 2008. Three types of sample plots were established: 1) unburned control with no harvesting (CO); 2) burned and uncleared (BU); and 3) burned and cleared (BC). Measurements were made in 2013, 21 years after wildfire in Vihterpalu and 5 years after wildfire in Nõva. Soil respiration ranged from 0.00 to 1.38 g CO2 m−2 h−1. Soil respiration in the burned and cleared areas (BC) was not reduced compared to burned and uncleared (BU) areas but the average soil respiration in unburned control areas was more than twice the value in burned areas (average soil respiration in CO areas was 0.34 CO2 m−2 h−1, versus 0.16 CO2 m−2 h−1, the average soil respiration of BC and BU combined). Recovery over 20 years was mixed; respiration was insignificantly lower on younger than older burned sites (when BC and BU values were combined, the average values were 0.15 vs. 0.17 g CO2 m−2 h−1, respectively); soil-C was greater in the older burned plots than the younger (when BC and BU values were combined, the average values were 9.71 vs. 5.99 kgm−2, respectively); but root biomass in older and recently burned areas was essentially the same (average 2.23 and 2.11 kgm−2, respectively); soil-N was highest on burned areas 20 years after fire. Twenty years post-fire may be insufficient time for carbon dynamics to fully recover on these low productivity sandy sites.
Authors
Eivind Vangdal Iren Lunde Knutsen Kristin Kvamm-LichtenfeldAbstract
European plums are susceptible to fruit cracking close to harvest. Heavy rainfall may lead to extensive damages leaving open wounds in the fruit flesh. In addition, cuticular fractures were found. Plum cultivar and stage of maturity are two major factors affecting the susceptibility to cracking. In order to reduce the plums’ susceptibility to cracking plum trees were treated with foliar fertilization during the growing season. Experiments included treatment with boron, calcium and nitrogen. Experiments including treatments with different levels of foliar fertilization did not show clear correlations between treatments and cracking in all cultivars. However, in some cultivars, more cuticular fractures were observed in fruits from nitrogen treated trees and less fractures in fruit from calcium or boron treated trees. In these experiments foliar fertilization with nitrogen, calcium or boron did not affect the amount of visible cracks in fruit significantly. Foliar fertilization is often shown to delay ripening. Even though fruit samples were picked at the same maturity stage, the effect of reduced cracking due to boron and calcium treatments could be partly an effect of differences in maturity. To make sure the fruits would develop fractures, unripe plum fruits on the trees were kept in a plastic bag with zip-lock and a few mL of water (to obtain 100% RH) for one week (from two to one week prior to estimated harvest date). In this way, the susceptibility of fruits on trees treated differently could be observed.
Authors
Sam Steyaert Shane Frank Stefano Puliti Rudy Badia Mie P. Arnberg Jack Beardsley Asle Økelsrud Rakel BlaalidAbstract
Cadaver decomposition islands around animal carcasses can facilitate establishment of various plant life. Facultative scavengers have great potential for endozoochory, and often aggregate around carcasses. Hence, they may disperse plant seeds that they ingest across the landscape towards cadaver decomposition islands. Here, we demonstrate this novel mechanism along a gradient of wild tundra reindeer carcasses. First, we show that the spatial distribution of scavenger faeces (birds and foxes) was concentrated around carcasses. Second, faeces of the predominant scavengers (corvids) commonly contained viable seeds of crowberry, a keystone species of the alpine tundra with predominantly vegetative reproduction. We suggest that cadaver decomposition islands function as endpoints for directed endozoochory by scavengers. Such a mechanism could be especially beneficial for species that rely on small-scale disturbances in soil and vegetation, such as several Nordic berry-producing species with cryptic generative reproduction.
Abstract
Inspired by the success of deep learning techniques in dense-label prediction and the increasing availability of high precision airborne light detection and ranging (LiDAR) data, we present a research process that compares a collection of well-proven semantic segmentation architectures based on the deep learning approach. Our investigation concludes with the proposition of some novel deep learning architectures for generating detailed land resource maps by employing a semantic segmentation approach. The contribution of our work is threefold. (1) First, we implement the multiclass version of the intersection-over-union (IoU) loss function that contributes to handling highly imbalanced datasets and preventing overfitting. (2) Thereafter, we propose a novel deep learning architecture integrating the deep atrous network architecture with the stochastic depth approach for speeding up the learning process, and impose a regularization effect. (3) Finally, we introduce an early fusion deep layer that combines image-based and LiDAR-derived features. In a benchmark study carried out using the Follo 2014 LiDAR data and the NIBIO AR5 land resources dataset, we compare our proposals to other deep learning architectures. A quantitative comparison shows that our best proposal provides more than 5% relative improvement in terms of mean intersection-over-union over the atrous network, providing a basis for a more frequent and improved use of LiDAR data for automatic land cover segmentation.
Abstract
Due to their Mendelian inheritance, microsatellites or SSRs (simple sequence repeats) can readily be used for parentage analyses of pear seedlings, thus revealing the female and male parents of the plant. However, in cases where obtained pear seeds display low viability, conducting a parentage analyses on the pear seeds themselves could be used in order to identify the male parent (successful pollinator). In this study, batches of seeds were extracted from fruits of the triploid ‘Ingeborg’ pear (‘Conference’ × ‘Bonne Louise’), the main commercial pear cultivar in Norway. Mature pears were collected from eight commercial pear orchards located in Ullensvang, at 60° North and seeds were collected. Genomic DNA was subsequently isolated from the obtained seed batches and genotyped using 12 microsatellite markers. The same markers were also used for the genetic characterization of ‘Ingeborg’ and five pear genotypes used as pollinators in the sampled orchards (‘Clara Frijs’, ‘Herzogine Elsa’, ‘Anna’, ‘Colorée de Juillet’ and ‘Belle lucrative’). The obtained SSR profiles were used in paternity analyses, as well as for gene assignment analyses. Both approaches identified pear ‘Clara Frijs’ to be the most successful pollinator in most of the sampled orchards. Subsequent S allele genotyping of ‘Ingeborg’ and five pear genotypes used as pollinators in the sampled orchards revealed that the preferential pollination by individual genotypes was not caused by gametophytic incompatibility or semi-compatibility.
Abstract
Climate change is expected to have a vigorous impact on soils and ecosystems due to elevated temperature and changes in precipitation (amount and frequency), thereby altering biogeochemical and hydrological cycles. Several phenomena associated with climate change and anthropogenic activity affect soils indirectly via ecosystem functioning (such as higher atmospheric CO2 concentration and N deposition). Continuous interactions between climate and soils determine the transformation and transport processes. Long-term gradual changes in abiotic environmental factors alter naturally occurring soil forming processes by modifying the soil water regime, mineral composition evolution, and the rate of organic matter formation and degradation. The resulting physical and chemical soil properties play a fundamental role in the productivity and environmental quality of cultivated land, so it is crucial to evaluate the potential outcomes of climate change and soil interactions. This paper attempts to review the underlying long-term processes influenced by different aspects of climate change. When considering major soil forming factors (climate, parent material, living organisms, topography), especially climate, we put special attention to soil physical properties (soil structure and texture, and consequential changes in soil hydrothermal regime), soil chemical properties (e.g. cation exchange capacity, soil organic matter content as influenced by changes in environmental conditions) and soil degradation as a result of longterm soil physicochemical transformations. The temperate region, specifically the Carpathian Basin as a heterogeneous territory consisting of different climatic and soil zones from continental to mountainous, is used as an example to present potential changes and to assess the effect of climate change on soils. The altered physicochemical and biological properties of soils require accentuated scientific attention, particularly with respect to significant feedback processes to climate and soil services such as food security.
Abstract
The Norwegian newly bred pear cultivar, Celina/QTee®, which was launched in 2010, has been released from the Norwegian breeding program that was initiated in 1983. It was derived from the combination ‘Colorée de Juillet’ × ‘Williams’. In Norway the flowering is medium to late and it ripens in the beginning of September. It has large attractive fruits with a red blush. It has a good fruit quality, storability and shelf life. Cross pollination is necessary in order to have high yields of this diploid cultivar. Pollination of ‘Celina’ with pollen of four donors (‘Conference’, ‘Kristina’, ‘Anna’ and ‘Fritjof’), together with open- and self-pollination were studied in this experiment during the 2016 season in Norway. The dynamics of the pollen tube growth (third, sixth and ninth day after anthesis) in style (upper, middle and lower third) and parts of the ovary in all crossing combination, were observed by fluorescent microscopy. Besides giving the best results regarding the average number of pollen tubes in different parts of pistils and the dynamics of pollen tube growth, ‘Conference’ was the only one of which the pollen tubes didn’t show any incompatible signs while growing through the transmitting tissue of the ‘Celina’ style. According to those preliminary results, ‘Conference’ was the best pollenizer, followed by ‘Kristina’. The study has to be repeated for another season.
Abstract
It is widely acknowledged that interdisciplinary research is required for adequately addressing global challenges. This article explores what interdisciplinary research implies for research libraries assisting such work, and for researchers receiving suppo rt. The main research question is: In what manner is interdisciplinary research support shaped and constructed as a result of contact and collaboration between researchers and the research library? Along with document studies, 15 semi - structured interviews have been conducted involving academic staff at the University of Oslo (UiO) and librarians at the UiO research library. Theoretical insight from the fields of Library and Information Science and Science and Technology Studies are combined using Boundary Objects (BO) as an analytical concept. In analysing empirical data, two dual - level competencies and library practices are identified: those that are technical and librarian, and those that are academically - professional and socio - emotional. In the junctions between these, interdisciplinary research support appears as a boundary object characterized as SubjectSocioTechnical. Collaboration and support for interdisciplinary research call for a complex of competencies, primarily because various support practices must be tailored to fit researchers’ disciplines and needs.
Authors
Shinsaku Sasai Keisuke Tamura Motoaki Tojo Maria-Luz Herrero Tamotsu Hoshino Satoshi T. Ohki Tomofumi MochizukiAbstract
We investigated virus infection in the oomycete Pythium polare from the Arctic. From 39 isolates investigated, 14 contained virus-like double-stranded RNA (dsRNA). Next generation sequencing revealed that the P. polare isolate OPU1176 contained three different virus-like sequences. We determined the full-length genome sequence of one of them. The 5397 nt-length genome had two overlapped open reading frames (ORFs) consistent with a toti and toti-like viruses, that we named Pythium polare RNA virus 1 (PpRV1). The ORF2 encoded an RNAdependent RNA polymerase (RdRp). The shifty heptamer motif and RNA pseudoknot were predicted near the stop codon of ORF1, implying that the RdRp could be translated as a fusion protein with the ORF1 protein. Phylogenetic analysis with deduced RdRp amino acid sequences indicated that oomycete virus PpRV1 was closely related to the unclassified arthropod toti-like viruses. The comparison of PpRV1-free and -infected lines suggested that PpRV1 infected in a symptomless manner.
Authors
Aaron M. Shew L. Lanier Nalley Heather A. Snell Rodolfo M. Jr. Nayga Bruce L. DixonAbstract
CRISPR gene-editing has major implications for agriculture and food security. However, no studies have evaluated the public acceptance and valuation of CRISPR-produced food. As such, we conducted a multi-country assessment of consumers’ willingness-to-consume (WTC) and willingness-to-pay (WTP) for CRISPR-produced food compared to conventional and genetically modified (GM) foods, respectively. In the USA, Canada, Belgium, France, and Australia, 56, 47, 46, 30, and 51% of respondents, respectively, indicated they would consume both GM and CRISPR food. We also found that biotechnology familiarity and perceptions of safety were the primary drivers for WTC CRISPR and GM food. Moreover, respondents valued CRISPR and GM food similarly – substantially less than conventional food – which could be detrimental for meeting future food demand.
Authors
Jarle W. Bjerke Ellen Elverland Laura Jaakola Leidulf Lund Bogdan Zagajewski Zbigniew Bochenek Andrzej Kłos Hans TømmervikAbstract
Climate change-induced snow thaw and subsequent accumulation of ice on the ground is a potential, major threat to snow-dominated ecosystems. While impacts of ground-ice on arctic wildlife are well explored, the impacts on tundra vegetation is far from understood. We therefore tested the vulnerability of two high-arctic plants, the prostrate shrub Salix polaris and the graminoid Luzula confusa, to ice encasement for 60 days under full environmental control. Both species were tolerant, showing only minor negative responses to the treatment. Subsequent exposure to simulated late spring frost increased the amount of damaged tissue, particularly in S. polaris, compared to the pre-frost situation. Wilting shoot tips of S. polaris increased nearly tenfold, while the proportion of wilted leaves of L. confusa increased by 15%. During recovery, damaged plants of S. polaris responded by extensive compensatory growth of new leaves that were much smaller than leaves of non-damaged shoots. The results suggest that S. polaris and L. confusa are rather tolerant to arctic winter-spring climate change, and this may be part of the reason for their wide distribution range and abundance in the Arctic.
Abstract
Effects of mitigation measures in agriculture on abating eutrophication are difficult to evaluate by assessments of catchment monitoring data. Estimates of improved water quality by specific agricultural Best Management Practices (BMPs) are therefore often dependent on simulation modeling. A main objective was thus to assess the probable reductions in total phosphorus (TP) loading achieved by implemented agricultural mitigation measures. The case-study site was a catchment in southeastern Norway. Simulation modeling was conducted by use of The Soil and Water Assessment Tool (SWAT). The aim of this present study was to understand the model uncertainty associated both with calibration/validation (baseline) and TP loading scenarios based on BMP. The modeled decrease in TP loading by the set of implemented BMPs was assessed by comparing simulated baseline output with output where the set of abatement actions were removed. The model was set up for the years 2006–2010 and calibrated against observed monitoring data, including daily discharge, sediment- and TP fluxes. Model simulations were performed including and excluding the implemented set of mitigation measures. The simulated set of mitigation measures include decrease in amount of phosphorus fertilization, establishment of vegetated buffer strips along streams and constructed wetlands in the water courses, no autumn tilling and removal of point TP sources from scattered dwellings. Model calibration and uncertainty estimation are performed using an algorithm for Sequential Uncertainty Fitting (SUFI2; ver. 2). Probabilistic risk for given magnitudes of increased TP loading if existing BMPs were not implemented was assessed. Using this novel approach it was possible to state, with a 80th percentile confidence level, that the average annual TP loading would have been about 26% higher if no mitigation measures were implemented in the catchment. This was possible to assess even though the difference between baseline and BMP scenario was not significant.
Authors
Calle Nilsson Henrik Ramebäck Callum Aidan Stephen Hill Mehrdad ArshadiAbstract
The self-heating of wood fuel pellets is a well-recognised problem causing fire incidents in the storage of the pellets as well as severe intoxication of workers by elevated carbon monoxide and carbon dioxide levels and oxygen depletion. Possible factors contributing to the self-heating are considered to be autoxidation and microbiological activity, while the role and contribution to the temperature rise caused by the heat of condensation from water vapour condensing during fast changes in the relative air humidity is less investigated. Using Dynamic Vapour Sorption, the water uptake was measured at 25 °C when increasing the RH from 40 to 80% using 35 fuel pellet samples covering a broad variation in pellet raw materials and process equipment found in Europe (both pilot and industrial scale). The equilibrium total water uptake and speed of the uptake were determined. Total water uptake was 4.56% (range 3.69–6.86%) with no systematic difference found related to the scale of production (industry as compared to pilot plant). In addition, the variations within larger groups of raw material (pine, spruce and pine/spruce mixtures) were relatively small, and the mean water uptake did not differ significantly between these groups. An estimation of the overall potential heat release (when raising the RH% from 40 to 80%) made from the experimental results, taking the early fast water uptake process into consideration (2 h counting for half the total uptake), showed that a heat release of 47 kJ/kg of pellets (range 12–63 kJ/kg) and a potential temperature increase of 45 °C is possible. This estimation clearly demonstrates that the heat of condensation released during water condensation in a pellets silo or in a pellets pile should be expected to be a major contributing factor to initiating temperature rise incidents. In addition, such a temperature increase is expected to assist the initiation of, and to increase the speed of autoxidation of fatty acids and other compounds in the material that will further contribute to a temperature rise. Thus, the results in this study have the potential to improve the basis for modelling the self-heating process in pellet silos/storage and to predict the status of a certain pellet batch by presenting a broad basis for expected variation in the important parameters (specific heat capacity CP and thermal conductivity λeff) influencing the process, and thus aid in taking preventive actions like venting or shifting the pellets to another silo/pile to reduce risk for self-heating and possible fire.
Authors
Björn Ringselle Therese With Berge Daniel Stout Tor Arvid Breland Paul E. Hatcher Espen Haugland Matthias Koesling Kjell Mangerud Tor Lunnan Lars Olav BrandsæterAbstract
Docks (Rumex spp.) are a considerable problem in grassland production worldwide. We investigated how different cultural management techniques affected dock populations during grassland renewal: (I) renewal time, (II) companion crop, (III) false seedbed, (IV) taproot cutting (V), plough skimmer and (VI) ploughing depth. Three factorial split-split plot experiments were carried out in Norway in 2007–2008 (three locations), 2008–2009 (one location) and 2009 (one location). After grassland renewal, more dock plants emerged from seeds than from roots. Summer renewal resulted in more dock seed and root plants than spring renewal. Adding a spring barley companion crop to the grassland crop often reduced dock density and biomass. A false seedbed resulted in 71% fewer dock seed plants following summer renewal, but tended to increase the number of dock plants after spring renewal. In some instances, taproot cutting resulted in less dock biomass, but the effect was weak and inconsistent, and if ploughing was shallow (16 cm) or omitted, it instead increased dock root plant emergence. Fewer root plants emerged after deep ploughing (24 cm) compared to shallow ploughing, and a plough skimmer tended to reduce the number further. We conclude that a competitive companion crop can assist in controlling both dock seed and root plants, but it is more important that the renewal time is favourable to the main crop. Taproot cutting in conjunction with ploughing is not an effective way to reduce dock root plants, but ploughing is more effective if it is deep and a skimmer is used.
Abstract
Beta-keratin in poultry feathers is a structural protein that is resistant to degradation due to disulfide and hydrogen bonds. Feather meal can be a valuable feed compound if the digestibility can be increased. The objective of the present study was to analyze the effects of chemical, enzymatic, and pressure-thermic treatments for chicken feathers on solubility, in vitro protein digestibility (IVPD), and amino acid composition of solubilized and residual fractions. Two experiments were conducted. In experiment 1, models for solubility and IVPD were developed including the above factors applying a central composite face-centered design. Addition of sodium hydroxide (NaOH) and sodium sulfite (Na2SO3), and autoclaving time affected solubility and IVPD of the feather hydrolysates, but not addition of keratinolytic enzyme. In experiment 2, 7 combinations of the hydrolysis factors NaOH, Na2SO3, and autoclaving time with a predicted IVPD of 900 g/kg of DM, calculated for the sum of solubilized and residual feather fractions, were included to measure effects on IVPD and amino acid composition in each fraction. The IVPD values were higher for solubilized than residual fractions when treated with NaOH and autoclaving, but no differences were found when treated with Na2SO3 and autoclaving. Losses of cystine were substantial for all treatments, but lower for Na2SO3 than for NaOH. Furthermore, use of lower Na2SO3 concentration and longer autoclaving time reduced losses of cystine. Compared with NaOH treatments, Na2SO3 gave lower losses of threonine, arginine, serine, and tyrosine. With reference to the ideal protein profile for Atlantic salmon (Salmo salar L.), the treatments with 60 or 90 min autoclaving and 0.36 or 0.21% Na2SO3 had the highest chemical scores. The scores were generally higher for amino acids in residual than solubilized fractions, but with 90 min autoclaving and 0.21% Na2SO3 differences were small. In conclusion, hydrolysis of chicken feathers with low concentrations of Na2SO3 combined with autoclaving results in feather meal with high nutritional value for Atlantic salmon; separation of solubilized and residual fractions is not necessary.
Authors
Francisco Javier Ancin Murguzur Aitor Barbero-Lopez Sari Kontunen-Soppela Antti HaapalaAbstract
Microbial growth on culture media is a commonplace technique to estimate the growth rate and virulence ofmicrobes, assess inhibitory effects of compounds and estimate potential damages of plant pathogens in agri-culture. Growth area measurement of solid cultures is still commonly performed as a manual process that re-quires skilled technicians and substantial time, thus warranting an automated system to reduce the workload andincrease measurement efficiency. A machine learning approach (Support Vector Machines) was developed tofully automate the area measurement process. We developed a functional model that processes images andreturns the microbial area coverage considerably faster than a manual measurement method, with minimal userinput and highly comparable results (R2= 0.88, kappa = 0.88) applicable over large datasets.
Authors
Alexandre Foito Derek StewartAbstract
Plants and crops contain a staggering diversity of compounds, many of which have pharmacological activity towards a variety of diseases. These properties have been exploited by traditional and modern medicine providing important sources of healthcare to this day. The contribution of natural products (such as plant-derived) to the modern pharmacopeia is indeed significant; however, the process of identifying novel bioactive compounds from biological sources has been a central challenge in the discovery of natural products. The resolution of these challenges relied extensively on the use of hyphenated Mass Spectrometry (MS) and Nuclear Magnetic Resonance (NMR)-based analytical technologies for the structural elucidation and annotation of novel compounds. Technical developments in instrumentation and data processing have fostered the development of the field of metabolomics which provides a wealth of tools with the huge potential for application in the process of drug/bioactive discovery from plant tissues. This manuscript provides an overview of the metabolomics toolbox available for the discovery of novel bioactive compounds and the integration of these tools in the bioprospection and drug discovery workflows.
Authors
Victor Phani Tagginahalli N. Shivakumara Keith Davies Uma RaoAbstract
Mucins are highly glycosylated polypeptides involved in many host–parasite interactions, but their function in plant-parasitic nematodes is still unknown. In this study, a mucin-like gene was cloned from Meloidogyne incognita (Mi-muc-1, 1125 bp) and characterized. The protein was found to be rich in serine and threonine with numerous O-glycosylation sites in the sequence. Quantitative real-time polymerase chain reaction (qRT-PCR) showed the highest expression in the adult female and in situhybridization revealed the localization of Mi-muc-1 mRNA ex-pression in the tail area in the region of the phasmid. Knockdown of Mi-muc-1 revealed a dual role: (1) immunologically, there was a significant decrease in attachment of Pasteuria penetrans en-dospores and a reduction in binding assays with human red blood cells (RBCs), suggesting that Mi-MUC-1 is a glycoprotein present on the surface coat of infective second-stage juveniles (J2s) and is involved in cellular adhesion to the cuticle of infective J2s; pretreatment of J2s with different carbohydrates indicated that the RBCs bind to J2 cuticle receptors different from those involved in the interaction of Pasteuria endospores with Mi-MUC-1; (2) the long-term effect of RNA interference (RNAi)-mediated knockdown of Mi-muc-1 led to a significant reduction in nematode fecundity, suggesting a possible function for this mucin as a mediator in the interaction between the nematode and the host plant.
Authors
Angela Koort Ulvi Moor Priit Poldma Clive Kaiser Marge StarastAbstract
The aim of the study was to determine the effect of modified atmosphere (MA) packages on the external quality of organically grown lowbush blueberry and half-highbush blueberry (’Northblue’) and the nutritional value of the fruits. Fruits were divided into plastic punnets and stored as follows: regular atmosphere (RA), punnets without packing; punnets sealed in a low-density polyethylene (LDPE, Estiko) bag; punnets sealed in an Xtend® blueberry bag (Stepac). Fruits were stored at 3 ± 1 ◦C. Compared to RA conditions, the Xtend® package prolonged the postharvest life for 15 days for lowbush and 9 days for half-highbush blueberries. Fruit dry matter (DM) and titratable acidity (TA) were higher in the Xtend® package. Fruit SSC decreased in the LDPE packages and increased in the Xtend® packages during storage. Based on the decreased soluble solids content (SSC) and titratable acidity (TA) ratio (SSC:TA) values during storage, it can be concluded that the taste of the fruits became sourer in all packages. Anthocyanin biosynthesis of lowbush blueberries was suppressed in MA, but this effect was not noticed for ‘Northblue’. Regarding fruit firmness, shrivelling, and decay, there were significant differences between the MA packages, but the genetic differences were more important: half-highbush blueberry fruits were firmer and less shrivelled.
Abstract
The fungus Neonectria fuckeliana has become an increasing problem on Norway spruce (Picea abies) in the Nordic countries during recent years. Canker wounds caused by the pathogen reduce timber quality and top-dieback is a problem for the Christmas tree industry. In this study, four inoculation trials were conducted to examine the ability of N. fuckeliana to cause disease on young Norway spruce plants and determine how different wound types would affect the occurrence and severity of the disease. Symptom development after 8–11 months was mainly mild and lesion lengths under bark were generally minor. However, N. fuckeliana could still be reisolated and/or molecularly detected. Slow disease development is in line with older studies describing N. fuckeliana as a weak pathogen. However, the results do not explain the serious increased damage by N. fuckeliana registered in Nordic forests and Christmas tree plantations. Potential management implications, such as shearing Christmas trees during periods of low inoculum pressure, cleaning secateurs between trees, and removal and burning of diseased branches and trees to avoid inoculum transfer and to keep disease pressure low, are based on experiments presented here and experiences with related pathogens.
Abstract
Phytophthora cryptogea, P. gonapodyides, P. lacustris, P. megasperma, P. plurivora, P. taxon paludosa and an unknown Phytophthora species were isolated from waterways and soil samples in Christmas tree fields in southern Sweden. In addition, P. megasperma was isolated from a diseased Norway spruce (Picea abies) plant from one of the fields in Svalöv. Inoculation tests were sequentially carried out with one isolate from each of the three species P. cryptogea, P. megasperma, and P. plurivora, all known pathogens on conifers. The same three isolates were used to study a few morphological features to confirm the identification, and temperature-growth relationships were carried out to see how well the organisms fit into Swedish climatic conditions. Seedlings of Norway spruce and Nordmann fir (Abies nordmanniana) were inoculated in the roots and the stems. None of the isolates caused extensive root rot under the experimental conditions, but all three species could be re-isolated from both Norway spruce and Nordmann fir. Phytophthora root rot is currently of minor concern for Christmas tree growers in Sweden. However, the Phytophthora isolations from soil and water indicate the presence of this damaging agent, which may lead to future problems.
Authors
Kaishan Song Zhidan Wen Yingxing Shang Hong Yang Lili Lyu Ge Liu Chong Fang Jia Du Ying ZhaoAbstract
As a major fraction of carbon in inland waters, dissolved organic carbon (DOC) plays a crucial role incarbon cycling on a global scale. However, the quantity of DOC stored in lakes and reservoirs was notclear to date. In an attempt to examine the factors that determine the DOC storage in lakes and reservoirsacross China, we assembled a large database (measured 367 lakes, and meta-analyzed 102 lakes from fivelimnetic regions; measured 144 reservoirs, and meta-analyzed 272 reservoirs from 31 provincial units) ofDOC concentrations and water storages for lakes and reservoirs that are used to determine DOC storagein static inland waters. We found that DOC concentrations in saline waters (Mean/median ± S.D: 50.5/30.0 ± 55.97 mg/L) are much higher than those in fresh waters (8.1/5.9 ± 6.8 mg/L), while lake DOCconcentrations (25.9/11.5 ± 42.04 mg/L) are much higher than those in reservoirs (5.0/3.8 ± 4.5 mg/L). Interms of lake water volume and DOC storage, the Tibet-Qinghai lake region has the largest water volume(552.8 km3), 92% of which is saline waters, thus the largest DOC (13.39 Tg) is stored in these alpine lakeregion; followed by the Mengxin lake region, having a water volume of 99.4 km3in which 1.75 Tg DOCwas stored. Compared to Mengxin lake region, almost the same amount of water was stored in East Chinalake region (91.9 km3), however, much less DOC was stored in this region (0.43 Tg) due to the lower DOCconcentration (Ave: 3.45 ± 2.68 mg/L). According to our investigation, Yungui and Northeast lake regionshad water storages of 32.14 km3and 19.44 km3respectively, but relatively less DOC was stored in Yungui(0.13 Tg) than in Northeast lake region (0.19 Tg). Due to low DOC concentration in reservoirs, especiallythese large reservoirs having lower DOC concentration (V > 1.0 km3: 2.31 ± 1.48 mg/L), only 1.54 Tg wasstored in a 485.1 km3volume of water contained in reservoirs across the entire country.
Abstract
Olfaction is the most important sensory mechanism by which many insects interact with their environment and a wind tunnel is an excellent tool to study insect chemical ecology. Insects can locate point sources in a three-dimensional environment through the sensory interaction and sophisticated behavior. The quantification of this behavior is a key element in the development of new tools for pest control and decision support. A wind tunnel with a suitable flight section with laminar air flow, visual cues for in-flight feedback and a variety of options for the application of odors can be used to measure complex behaviour which subsequently may allow the identification of attractive or repellent odors, insect flight characteristics, visual-odor interactions and interactions between attractants and odors lingering as background odors in the environment. A wind tunnel holds the advantage of studying the odor mediated behavioural repertoire of an insect in a laboratory setting. Behavioural measures in a controlled setting provide the link between the insect physiology and field application. A wind tunnel must be a flexible tool and should easily support the changes to setup and hardware to fit different research questions. The major disadvantage to the wind tunnel setup described here, is the clean odor background which necessitates special attention when developing a synthetic volatile blend for field application.
Authors
Ievina Sturite Arta Kronberga Vija Strazdina Aina Kokare Mauritz Åssveen Anne Kari Bergjord Olsen Vita Sterna Evita StraumiteAbstract
Multilocation testing remains the main tool for understanding varietal responses to the environment. Here, Latvian and Norwegian hull-less and hulled barley varieties were tested in field experiments in Latvia and Norway in order to assess the varieties adaptability across environments (sites). Two Latvian (cv Irbe and cv Kornelija) and one Norwegian hull-less barley variety (cv Pihl) were tested along with one Latvian (cv Rubiola) and one Norwegian hulled barley variety (cv Tyra) under conventional and organic management systems. The grain yield, together with physical and chemical grain parameters were compared, and variety yield and protein stability detemined. Overall, grain yield of hull-less barley varieties was significantly lower than for hulled barley varieties regardless of climatic conditions and management system. However, in the organic farming systems this difference between barley types was less pronounced. The hull-less barley varieties cv Pihl and cv Irbe, along with both hulled varieties, had good yield stability across environments and were well adapted to both cropping systems. Hull-less barley varieties tended to contain more protein and β -glucans than hulled barley varieties. Despite being bred for local conditions in Norway and Latvia, our study shows that all the varieties used may be successfully transferred across countries.
Abstract
There is increasing evidence that the impact of climate change on the productivity of grasslands will at least partly depend on their biodiversity. A high level of biodiversity may confer stability to grassland ecosystems against environmental change, but there are also direct effects of biodiversity on the quantity and quality of grassland productivity. To explain the manifold interactions, and to predict future climatic responses, models may be used. However, models designed for studying the interaction between biodiversity and productivity tend to be structurally different from models for studying the effects of climatic impacts. Here we review the literature on the impacts of climate change on biodiversity and productivity of grasslands. We first discuss the availability of data for model development. Then we analyse strengths and weaknesses of three types of model: ecological, process-based and integrated. We discuss the merits of this model diversity and the scope for merging different model types.
Abstract
Yunnan pine is the most important tree species in SW China in both economical and ecological terms, but it is often killed by pine shoot beetles (Tomicus spp.). Tomicus beetles are secondary pests in temperate regions and the aggressiveness of the beetles in SW China is considered to be due to the warm subtropical climates as well as the beetles’ virulent fungal associates. Here, we assessed the virulence of three blue-stain fungi (Leptographium wushanense, L. sinense and Ophiostoma canum) associated with pine shoot beetles to Yunnan pine (Pinus yunnanensis) in SW China. Following fungal inoculation, we measured necrotic lesion lengths, antioxidant enzyme activities and monoterpene concentrations in the stem phloem of Yunnan pine. Leptographium wushanense induced twice as long lesions as L. sinense and O. canum, and all three fungi induced significantly longer lesions than sterile agar control inoculations. The activity of three tested antioxidant enzymes (peroxidase, polyphenol oxidase, and superoxide dismutase) increased after both fungal inoculation and control inoculation. However, L. wushanense and L. sinense generally caused a greater increase in enzyme activities than O. canum and the control treatment. Fungal inoculation induced stronger increases in six major monoterpenes than the control treatment, but the difference was significant only for some fungus-monoterpene combinations. Overall, our results show that L. wushanense and L. sinense elicit stronger defense responses and thus are more virulent to Yunnan pine than O. canum. The two Leptographium species may thus contribute to the aggressiveness of their beetle vectors and could damage Yunnan pine across SW China if they spread from the restricted geographical area they have been found in so far.
Abstract
The aim of this research is to determine if the polyesterification of sorbitol and citric acid in wood has a future potential as a wood modification process. Pine wood was impregnated with an aqueous solution containing citric acid and sorbitol and was thereafter cured at 103 or 140°C for 18 hours. The dimensional stability and leaching resistance were studied for both modification temperatures. The leachates from the modified wood samples were analysed by HPLC and the susceptibility to decay and staining fungi were studied. Impregnated samples cured at 140°C showed a permanent (leach-resistant) increased dimensional change, but samples treated at 103°C were not stable to leaching. Treated samples cured at 103 and 140°C showed significant resistance to white-rot (Trametes versicolor) and brown-rot decay (Postia placenta) after a leaching procedure. Furthermore, samples cured at 103 and 140°C (leached and unleached) were significantly less susceptible to blue-stain fungi than the untreated controls.
Authors
Di Zeng Michael R. Thomsen Rodolfo M. Jr. Nayga Heather L. RouseAbstract
The association between the commercial food environment and childhood obesity is increasingly assessed in the literature, but little is known about the role of convenience stores, an important food retail format worldwide. This study helps bridge the gap using individual-level data containing measured body mass index (BMI) for public schoolchildren and geo-coded residence and store locations in Arkansas, United States. The distance from residence to the nearest highway is employed to instrument neighbourhood convenience store exposure, while controlling for possible confounding effects of other food stores. We find that exposure to at least one convenience store exposure is associated with a BMI z-score increase of 0.162 SD, and exposure to each additional convenience store is associated with a BMI increase of 0.071 SD. There is no evidence for a larger association among children from low-income families or those with limited access to healthy foods.
Abstract
Increasing abundance of Juncus effusus (soft rush) and Juncus conglomeratus (compact rush) in pastures and meadows in western Norway has caused reductions in forage yield and quality in recent decades. Understanding plant development and regrowth following cutting is essential in devising cost-effective means to control rushes. In a field experiment in western Norway, we investigated development of above- and below-ground fractions of rush from seedlings to three-year-old plants, including the impact on vigour of disturbing growth by different cutting frequencies during the period 2009–2012. Each year, the plants were exposed to one or two annual cuts or left untreated and five destructive samplings were performed from March to early December. Juncus effusus showed significantly more vigorous growth than Juncus conglomeratus in the last two years of the study period. The above-ground:below-ground biomass ratio of both species increased mainly in spring and early summer and was reduced in late summer and autumn. Removal of aerial shoots also reduced the below-ground fraction of both species. One annual cut in July effectively reduced biomass production in both species by 30–82%, which was only a slightly smaller reduction than with two annual cuts, in June and August. Mechanical control measures such as cutting can thus effectively reduce rush vigour when performed late in the growing season.
Abstract
This study examines the relationships between profitability, nitrogen (N) surplus, greenhouse gas emissions (GHG), and energy intensity and factors influencing these relationships in dairy farming. In-depth data from 10 conventional and 8 organic dairy farms in Western Norway were analyzed. Organic farms had lower N surplus per hectare (local, onfarm) and per unit output (global, cradle-to-farm-gate), and lower estimated GHG emissions and energy intensity per unit output, whereas labor input and farm profits did not differ. Higher profitability tended to be associated with improved performance of the environmental indicators examined. Intensification through increased use of concentrates tended to improve profit and reduce N surplus, GHG emissions, and energy intensity per unit output within each farming system while N surplus per hectare could be negatively affected. To ensure a balanced representation of the environmental consequences of both organic and conventional farming systems,our results give support to extensive examination of both area and product-based environmental performance indicators.
Authors
John Koestel Annette Dathe Todd H. Skaggs Ove Mindor Klakegg Muhammad Arslan Ahmad Maryia Babko Daniel Giménez Csilla Farkas Attila Nemes Nicholas JarvisAbstract
The saturated hydraulic conductivity of soil, Ks, is a critical parameter in hydrological models that remains notoriously difficult to predict. In this study, we test the capability of a model based on percolation theory and critical path analysis to estimate Ks measured on 95 undisturbed soil cores collected from contrasting soil types. One parameter (the pore geometry factor) was derived by model fitting, while the remaining two parameters (the critical pore diameter, dc, and the effective porosity) were derived from X‐ray computed tomography measurements. The model gave a highly significant fit to the Ks measurements (p < 0.0001) although only ~47% of the variation was explained and the fitted pore geometry factor was approximately 1 to 2 orders of magnitude larger than various theoretical values obtained for idealized porous media and pore network models. Apart from assumptions in the model that might not hold in reality, this could also be attributed to experimental error induced by, for example, air entrapment and changes in the soil pore structure occurring during sample presaturation and the measurement of Ks. Variation in the critical pore diameter, dc, was the dominant source of variation in Ks, which suggests that dc is a suitable length scale for predicting soil permeability. Thus, from the point of view of pedotransfer functions, it could be worthwhile to direct future research toward exploring the correlations of dc with basic soil properties and site attributes.
Authors
Mélanie Harzé Arnaud Monty Sylvain Boisson Carline Pitz Julia-Maria Hermann Johannes Kollmann Grégory MahyAbstract
Persistence of restored populations depends on growth, reproduction, dispersal, local adaptation, and a suitable landscape pattern to foster metapopulation dynamics. Although the negative effects of habitat fragmentation on plant population dynamics are well understood, particularly in grasslands, the population traits that control grassland restoration are less known. We reviewed the use of population traits for evaluating grassland restoration success based on 141 publications (1986–2015). The results demonstrated that population demography was relatively well‐assessed but detailed studies providing information on key stages of the life cycle were lacking despite their importance in determining population viability. Vegetative and generative performances have been thoroughly investigated, notably the components of plant fitness, such as reproductive output, while genetic and spatial population structures were largely ignored. More work on the population effects of ecological restoration would be welcomed, particularly with a focus on population genetics. Targeted species were principally common and dominant natives, or invasive plants while rare or threatened species were poorly considered. Evaluation of ecological restoration should be conducted at different scales of ecological complexity, but so far, communities and ecosystems are over represented, and more focus should be directed towards a population approach as population traits are essential indicators of restoration success.
Authors
Katharina Strobl Claudia Schmidt Johannes KollmannAbstract
Phytometers are indicator transplants that provide information on site conditions based on plant survival,growth and reproduction. Since this is a relatively new approach, standards for its implementation remain to bedefined, for example, during peatland restoration. Peatland restoration frequently aims at recovering char-acteristic communities, and a key attribute of successfully restored ecosystems is their capacity to sustain viablepopulations of target species. When not actively introduced, these species are expected to establish on their ownafter improving site conditions, for example by rewetting. Assessments to determine whether this goal is metrequire the long-term monitoring of species’ presence, whereas the underlying causes of these observations, i.e.site or dispersal limitation, often remain unknown. Using phytometers within ecological restoration helps ad-dressing this question. The goal of this study is to compare the responses of several species and traits to en-vironmental conditions in restored peatlands. Three target species (Drosera rotundifolia, Eriophorum vaginatum,Vaccinium oxycoccos) were planted in restored montane peatlands in central Germany, while in a greenhouseexperiment, the same species were grown on peat from the field sites and exposed to two water levels. Severalplant traits were measured and compared with variation in light, water and soil conditions. The response tohabitat conditions was species-specific, indicating that the use of different phytometers increases the reliabilityof monitoring. Survival and growth traits were suitable to assess a wide range of abiotic conditions, whiledifferences in reproductive output were more time-consuming to measure. Survival provided the most conclusiveresults for species sensitive to stressful habitat conditions. Biomass and other size metrics of the phytometers, aswell as growth and reproductive traits were partly redundant. Thus, we suggest recording survival and biomassand use non-destructive growth measurements for repeated assessments, while the choice of the most suitablesize trait should depend on the growth form. Our study stresses the potential of phytometers for monitoring therestoration outcome, while highlighting the importance of species and trait selection.
Abstract
Purpose – The purpose of this paper is to explore the economic performance of Norwegian crop farms using a stochastic frontier analysis. Design/methodology/approach – The analysis was based on a translog cost function and unbalanced farm-level panel data for 1991–2013 from 455 Norwegian farms specialized in crop production in eastern and central regions of Norway. Findings – The results of the analysis show that the mean efficiency was about 78–81 percent. Farm management practices and socioeconomic factors were shown to significantly affect the economic performance of Norwegian crop farms. Research limitations/implications – Farmers are getting different types of support from the government and the study does not account for the different effects of different kinds of subsidy on cost efficiency. Different subsidies might have different effects on farm performance. To get more informative and useful results, it would be necessary to repeat the analysis with less aggregated data on subsidy payments. Practical implications – One implication for farmers (and their advisers) is that many of them are less efficient than the estimated benchmark (best performing farms). Thus, those lagging behind the best performing farms need to look at the way they are operating and to seek out ways to save costs or increase crop production. Perhaps there are things for lagging farmers to learn from their more productive farming neighbors. For instance, those farmers not practicing crop rotation might be well advised to try that practice. Social implications – For both taxpayers and consumers, one implication is that the contributions they pay that go to subsidize farmers appear to bring some benefits in terms of more efficient production that, in turn, increase the supply of some foods so possibly making food prices more affordable. Originality/value – Unlike previous performance studies in the literature, the authors estimated farm-level economic performance accounting for the contribution of both an important farm management practice and selected socioeconomic factors. Good farm management practices, captured through crop rotation, land tenure, government support and off-farm activities were found to have made a positive and statistically significant contribution to reducing the cost of production on crop-producing farms in the Central and Eastern regions of Norway.
Abstract
The effect of controlled nutrient feeding during the period of short day (SD) induction of flowering has been studied in three SD berry crops. An experimental system with standardized plant material grown with trickle fertigation in controlled environments was used. In strawberry, flowering was advanced and increased when an additional N pulse was given 1-2 weeks after commencement of a 4-week SD induction period, while the opposite resulted when the treatment was applied 2 weeks before start of SD. In blackcurrant, the highest flowering and yield were obtained when fertilization was applied shortly after the natural photoperiod had declined to the inductive length in September. While generous nutrient supply during spring and summer reduced berry soluble solids in blackcurrant, this was not observed with autumn fertilization. Autumn fertilization did not adversely affect plant winter survival or growth vigour in spring. Withdrawal of fertilization prior to, or at various stages during floral induction, did not significantly affect flowering and yield in raspberry, but marginally advanced flowering and fruit ripening.
Abstract
The amount of rented farmland in Norway has increased steadily since the 1950s. Concerns have been raised questioning whether farmland is treated less well by tenants compared to landowners. This study aims to investigate how farmers perceive their treatment of rented farmland, which factors impact their decisionmaking related to this and if farmers are concerned about farmland elements that are less important for productivity but mainly of interest for cultural heritage or environmental management reasons. Semi-structured interviews with a group of randomly selected farmers were carried out in an area dominated by intensive agriculture. Independent of, for example, amount of rented land or duration of the rental agreement, all farmers agreed that rented land was treated well. A strong competition for farmland in combination with farmers being dependent on renting land was the most important reason. Results from this study may be transferrable to other farming areas, at least where competition for farmland is comparable. We do suggest, however, that any further research on treatment of rented farmland in Norway should take a regional approach, since national statistics may cover significant regional differences.
Abstract
A large proportion of global agricultural soils contain suboptimal available phosphorus (P) for the growth of many plant species. Boron (B) plays important roles in plant growth and development, but limited research has been conducted to study B uptake under low P availability. This study comprised a hydroponic and a mini-rhizobox experiment with canola (Brassica napus L.), potato (Solanum tuberosum L.) and wheat (Triticum aestivum L.) under P sufficient and deficient conditions. Boron concentrations, rhizosphere soil pH, and gene expression of BnBOR1 in canola were determined. Shoot B concentrations were found significantly increased (11–149%) by low P availability in potato and canola but not in wheat. Reverse transcription polymerase chain reaction (RT-PCR) indicated that BnBOR1;2a, BnBOR1;2c, and BnBOR1;3c were up-regulated after seven days of low P treatment in canola roots. Our results indicate that plant shoot B concentration was dramatically influenced by P availability, and dicots and monocots showed a contrasting B concentration response to low P availability.
Abstract
In spite of its important role as predator of small game species, estimating the density of red fox Vulpes vulpes has been hampered by the species’ highly variable ranging pattern and elusive behavior. DNA analysis from scats combined with spatially explicit capture–recapture (SECR) modeling might remedy this. In a 50-km2 coniferous forest in southeast Norway, we collected scats on logging roads in late winter. DNA was extracted, amplified, and genotyped using 11 microsatellite markers. Of 184 samples collected, 126 were genotyped successfully, of which 46 (36.5%) produced individual genetic profiles. Twenty-five of these were different individuals: 13 females and 12 males. Nine of them were identified in multiple scats; mean recapture rate among all was 1.8/animal. Applying a conventional capture–recapture model (CAPWIRE) to the genotyped samples, 36 (95% CI 26–52) different individuals were estimated to have been present in the area during the sampling period. For estimating population density, we constructed three differently sized occupancy areas based on distances between recaptures, viz. ½ and 1/1 mean maximum distance moved (MMDM) and the local convex hull home range method (LoCoH). Areas varied from 60 km2 (½MMDM) to 112 km2 (MMDM), producing density estimates of 0.60 and 0.32 foxes/km2, respectively; the 95% LoCoH range method produced an estimate of 0.44 animals/km2 . Based on SECR modeling, the density was estimated at 0.38 (95% CI 0.21–0.70) animals/km2 . Smaller confidence intervals are expected with more appropriate sampling design than used in this pilot study.
Authors
Habtamu AlemAbstract
This paper examines the recent advances in stochastic frontier models and its implications for the performance of the Norwegian crop producing farms. Unlike the previous studies, we used a cost function in multiple input-output frameworks to estimate both long-run (persistent) and short-run (transient) inefficiency. The empirical analysis is based on unbalanced farm-level panel data for 1991-2013 from 455 Norwegian farms specialized in crop production with 3885 observations. We estimated seven SF panel data models grouped into four categories regarding the assumptions used to the nature of inefficiency. The estimated cost efficiency scores varied from 53 % to 95%, showing that the results are sensitive to how the inefficiency is modeled and interpreted. Keywords: cost function, short and long-run inefficiency, agriculture, panel data
Authors
Roger Holten Frederik Bøe Marit Almvik Sheela Katuwal Marianne Stenrød Mats Larsbo Nicholas Jarvis Ole Martin EkloAbstract
Limited knowledge and experimental data exist on pesticide leaching through partially frozen soil. The objective of this study was to better understand the complex processes of freezing and thawing and the effects these processes have on water flow and pesticide transport through soil. To achieve this we conducted a soil column irrigation experiment to quantify the transport of a non-reactive tracer and the herbicide MCPA in partially frozen soil. In total 40 intact topsoil and subsoil columns from two agricultural fields with contrasting soil types (silt and loam) in South-East Norway were used in this experiment. MCPA and bromide were applied on top of all columns. Half the columns were then frozen at −3 °C while the other half of the columns were stored at +4 °C. Columns were then subjected to repeated irrigation events at a rate of 5 mm artificial rainwater for 5 h at each event. Each irrigation was followed by 14-day periods of freezing or refrigeration. Percolate was collected and analysed for MCPA and bromide. The results show that nearly 100% more MCPA leached from frozen than unfrozen topsoil columns of Hov silt and Kroer loam soils. Leaching patterns of bromide and MCPA were very similar in frozen columns with high concentrations and clear peaks early in the irrigation process, and with lower concentrations leaching at later stages. Hardly any MCPA leached from unfrozen topsoil columns (0.4–0.5% of applied amount) and concentrations were very low. Bromide showed a different flow pattern indicating a more uniform advective-dispersive transport process in the unfrozen columns with higher con- centrations leaching but without clear concentration peaks. This study documents that pesticides can be pre- ferentially transported through soil macropores at relatively high concentrations in partially frozen soil. These findings indicate, that monitoring programs should include sampling during snow melt or early spring in areas were soil frost is common as this period could imply exposure peaks in groundwater or surface water.
Authors
Maryia Khomich Filipa Cox Carrie Joy Andrew Tom Andersen Håvard Kauserud Marie Louise DaveyAbstract
Insufficient reference database coverage is a widely recognized limitation of molecular ecology ap-proaches which are reliant on database matches for assignment of function or identity. Here, we use datafrom 65 amplicon high-throughput sequencing (HTS) datasets targeting the internal transcribed spacer(ITS) region of fungal rDNA to identify substrates and geographic areas whose underrepresentation in theavailable reference databases could have meaningful impact on our ability to draw ecological conclu-sions. A total of 14 different substrates were investigated. Database representation was particularly poorfor the fungal communities found in aquatic (freshwater and marine) and soil ecosystems. Aquaticecosystems are identified as priority targets for the recovery of novel fungal lineages. A subset of the datarepresenting soil samples with global distribution were used to identify geographic locations andterrestrial biomes with poor database representation. Database coverage was especially poor in tropical,subtropical, and Antarctic latitudes, and the Amazon, Southeast Asia, Australasia, and the Indian sub-continent are identified as priority areas for improving database coverage in fungi.
Authors
Marcin Strozecki Hanna Marika Silvennoinen Pawel Strzelinski Bogdan Heronim ChojnickiAbstract
It is important to quantify carbon decomposition to assess the reforestation impact on the forest floor C stocks. Estimating the loss of C stock in a short-term perspective requires measuring changes in soil respiration. This is not trivial due to the contribution of both soil microbes and vegetation to the measured CO2 flux. However, C stable isotopes can be used to partition the respiration and potentially to assess how much of the recalcitrant C stock in the forest floor is lost. Here, we measured the soil respiration at two forest sites where different regeneration methods were applied, along with an intact forest soil for reference. In so doing, we used a closed dynamic chamber for measuring respiration and the 13C composition of the emitted CO2. The chamber measurements were then supplemented with the soil organic carbon analysis and its δ13C content. The mean δ13C-CO2 estimates for the source of the CO2 were -26.4, -27.9 and -29.5‰, for the forest, unploughed and ploughed, respectively. The 13C of the soil organic carbon did, not differ significantly between sites. The higher soil respiration rate at the forest, as compared to the unploughed site, could be attributed to the autotrophic respiration by the forest floor vegetation.
Abstract
The present work focuses on an assessment of the applicability of groundwater table (GWT) measures in the modelling of soil water retention characteristics (SWRC) using artificial neural network (ANN) methods. Model development, testing, validation and verification were performed using data collected across two decades from soil profiles at full-scale research objects located in Southwest Poland. A positive effect was observed between the initial GWT position data and the accuracy of soil water reserve estimation. On the other hand, no significant effects were observed following the implementation of GWT fluctuation data over the entire growing season. The ANN tests that used data of either soil water content or GWT position gave analogous results. This revealed that the easily obtained data (temperature, precipitation and GWT position) are the most accurate modelling parameters. These outcomes can be used to simplify modelling input data/parameters/variables in the practical implementation of the proposed SWRC modelling variants.
Abstract
Uganda designated 16% of its land as Protected Area (PA). The original goal was natural resources, habitat and biodiversity conservation. However, PAs also offer great potential for carbon conservation in the context of climate change mitigation. Drawing on a wall-to-wall map of forest carbon change for the entire Uganda, that was developed using two Digital Elevation Model (DEM) datasets for the period 2000–2012, we (1) quantified forest carbon gain and loss within 713 PAs and their external buffer zones, (2) tested variations in forest carbon change among management categories, and (3) evaluated the effectiveness of PAs and the prevalence of local leakage in terms of forest carbon. The net annual forest carbon gain in PAs of Uganda was 0.22 ± 1.36 t/ha, but a significant proportion (63%) of the PAs exhibited a net carbon loss. Further, carbon gain and loss varied significantly among management categories. About 37% of the PAs were “effective”, i.e., gained or at least maintained forest carbon during the period. Nevertheless, carbon losses in the external buffer zones of those effective PAs significantly contrast with carbon gains inside of the PA boundaries, providing evidence of leakage and thus, isolation. The combined carbon losses inside the boundaries of a large number of PAs, together with leakage in external buffer zones suggest that PAs, regardless of the management categories, are threatened by deforestation and forest degradation. If Uganda will have to benefit from carbon conservation from its large number of PAs through climate change mitigation mechanisms such as REDD+, there is an urgent need to look into some of the current PA management approaches, and design protection strategies that account for the surrounding landscapes and communities outside of the PAs.
Abstract
The long-term success of sites selected for species conservation depends on the persistence of target species. Red List species or threatened species lists are frequently defined as target species, but when Red Lists are updated, their species composition may change. Here we investigate the effects of Red List updates on the long-term robustness of fine-scale site selection. We used records of red-listed species (vascular plants, bryophytes, macrolichens, and polypore fungi) recorded in 1997–1998 in 1058 sample plots (50 × 50 m) from six forest landscapes in Norway, and four consecutive issues of the Norwegian Red List for species (1998, 2006, 2010, 2015). Sites were selected based on the first issue (1998) using both a scoring (“hotspot”) approach and a complementarity approach, and the ability of selected sites to include red-listed species of later issues was measured. In four boreal forests the mean proportion of red-listed species included in selected sites were reduced by18% during the study period, whereas no such effect was found in two hemiboreal forests, where increased clustering of red-listed species in sites compensated for target species changes. Changing target species adds to earlier documented challenges caused by population dynamics, and we suggest that alternatives to using occurrences of target species in site selection should be considered, and particularly at finer spatial scales.
Authors
Jing-Wei Li Min-Rui Wang Hai-Yan Chen Lei Zhao Zhen-Hua Cui Zhibo Hamborg Dag-Ragnar Blystad Qiao-Chun WangAbstract
Availability of and easy access to diverse plant viruses and viroids is a prerequisite in applied and basic studies related to viruses and viroids. Long-term preservation of viruses and viroids is difficult. A protocol was described for long-term preservation of potato leafroll virus (PLRV), potato virus S (PVS), and potato spindle tuber viroid (PSTVd) in cryopreserved shoot tips of potato cv. Zihuabai. Shoot regrowth levels following cryopreservation were higher in 1.5 mm-shoot tips (58–60%) than in 0.5-mm-ones (30–38%). All shoots recovered from 0.5-mm-shoot tips were PVS- and PSTVd-preserved, but none of them were PLRVpreserved. Cryopreservation of 1.5-mm-shoot tips resulted in 35% and 100% of PLRV- and PVS- and PSTVd-preserved shoots. Studies on cell survival patterns and virus localization provided explanations to the varying PLRV-preservation frequencies produced by cryopreservation of the two sizes of shoot tips. Although micropropagation efficiencies were low after 12 weeks of subculture following cryopreservation, similar efficiencies were obtained after 16 weeks of subculture in pathogen-preserved shoots recovered from cryopreservation, compared with the diseased in vitro stock shoots (the control). Pathogen concentrations in the three pathogens-preserved shoots analyzed by qRT-PCR were similar to those in micropropagated shoots. The three pathogens cryopreserved in shoot tips were readily transmitted by grafting and mechanical inoculation to potato plants. PLRV, PVS, and PSTVd represent a diverse range of plant viruses and viroid in terms of taxonomy and infectious ability. Therefore, shoot tip cryopreservation opens a new avenue for long-term preservation of the virus and viroid.
Authors
Kalev Jogiste Lee E. Frelich Diana Laarmann Floortje Vodde Endijs Baders Janis Donis Aris Jansons Ahto Kangur Henn Korjus Kajar Köster Jürgen Kusmin Timo Kuuluvainen Vitas Marozas Marek Metslaid Sandra Metslaid Olga Polyachenko Anneli Poska Sille Rebane John A. StanturfAbstract
In the Baltic States region, anthropogenic disturbances at different temporal and spatial scales mostly determine dynamics and development phases of forest ecosystems. We reviewed the state and condition of hemiboreal forests of the Baltic States region and analyzed species composition of recently established and permanent forest (PF). Agricultural deforestation and spontaneous or artificial conversion back to forest is a scenario leading to ecosystems designated as recent forest (RF, age up to two hundred years). Permanent forest (PF) was defined as areas with no records of agricultural activity during the last 200 yr, including mostly forests managed by traditional even-aged (clear-cut) silviculture and salvage after natural disturbances. We hypothesized that RF would have distinctive composition, with higher dominance by hardwoods (e.g., aspen and birch), compared to PF. Ordination revealed divergence in the RF stands; about half had the hypothesized composition distinct from PF, with a tight cluster of stands in the part of the ordination space with high hardwood dominance, while the remaining RF stands were scattered throughout the ordination space occupied by PF with highly variable species composition. Planting of conifers, variability in site quality, and variability in spatial proximity to PF with relatively natural ecosystem legacies likely explained the variable compositions of this latter group of RF. We positioned the observations of RF in a classic quantification of site type conditions (based on Estonian forest vegetation survey previously carried out by L~ohmus), which indicated that RF was more likely to occur on areas of higher soil fertility (in ordination space). Climatic and anthropogenic changes to RF create complex dynamic trends that are difficult to project into the future. Further research in tracing land use changes (using pollen analysis and documented evidence) should be utilized to refine the conceptual framework of ecosystem legacy and memory. Occurrence and frequency of deforestation and its characteristics as a novel disturbance regime are of particular interest.
Authors
Kar H. Lim Wuyang Hu Rodolfo M. Jr. NaygaAbstract
The Marine Stewardship Council's (MSC) sustainable seafood ecolabel covers about 10% of total seafood catch globally. Despite its prevalence, consumer willingness to pay (WTP) for MSC-certified imported seafood is not well understood. Using a choice experiment conducted with an American-consumers sample, this study measures the differences in WTP for American, Ecuadorian, and Vietnamese canned tuna. The results noted two things. First, the ecolabel induces country-specific effects, where the marginal WTP for the MSC label is higher for the imported products than for the domestic product; second, consumers prefer domestic products ceteris paribus, nevertheless, the premium of the ecolabel—when attached to the imported products—may partially eclipse preference for domestic products without the ecolabel. The results imply that the MSC ecolabel may generate a more favorable effect when applied to products from developing countries.
Authors
M. Bauer R. Harzer K. Strobl Johannes KollmannAbstract
River restoration is widely applied, although its effects are poorly understood, and degraded habitats might be difficult to improve. Moreover, there is a lack of monitoring as well as few systematic comparisons of restoration methods. This study presents results of a 4‐year monitoring on River Inn (southern Germany) investigating restoration by gravel or sand addition or embankment removal. The results were compared with reference sites that represent the pre‐restoration conditions. At the landscape scale, we analysed vegetation types based on aerial photographs, whereas at a smaller scale, we undertook vegetation surveys and evaluated species composition, growth, and life form, as well as the proportion of the target vegetation. After 4 years, the data indicated a “negative resilience” of the vegetation back to the state prior to restoration. The structural analysis revealed an extensive spread of reed at expense of bare soil. Thus, the species composition largely regressed to the pre‐restoration conditions, and neither annuals nor other pioneer species showed a long‐term benefit of river restoration. There were differences among the three restoration treatments after 2 years, but no longer after 4 years. However, the river restoration had three positive outcomes: (a) There was a temporary benefit for pioneer vegetation that most likely replenished the seed bank of the respective species, (b) the valuable reed communities showed resilience, and (c) the measures allowed some practical learning as expected for adaptive restoration.
Abstract
European plums (Prunus domestica L.) blossom abundantly most years and often set too many flowers. If these excessive numbers of fruitlets remain on the trees until harvest, the crop would consist of small, unmarketable fruits of low fruit quality. Thinning agents like ammoniumthiosulphate (ATS), sulphur and soya oil desiccate flowers, especially stigma, which is the most sensitive tissue part of the flower. This way, the main effect of blossom thinning treatments is the disruption of pollination and fertilization. Thinning trials were conducted at a commercial orchard near the shore of the Hardangerfjord near Nibio Ullensvang, western Norway (60.2°N) on mature ‘Jubileum’ trees, all grafted on ‘St. Julien A’ rootstock. The trees were treated with 0.4, 0.8, 2 and 4% sulphur; a mixture of 0.4% sulphur + 2% soya oil and 1.5% ATS (liquid and powder formulations), at full bloom. All treatments were compared with hand-thinned and unthinned trees which were used as a control. Experimental trees were sprayed to the point of run-off with a hand sprayer during May 2008 and 2009 at full bloom. Flower thinners were efficient at relatively low temperatures which is a benefit in a cooler climate. In 2008 all thinning treatments reduced fruit set compared to unthinned controls for all cultivars. Sulphur and soya oil, both alone and in combination, were significantly less effective than ATS. In 2009, fruit set was higher and the effects of all thinning agents were lower. Fruit set decreased with increasing sulphur concentrations, but fruit thinning was not sufficient, even at the highest concentration. Both the liquid and powder formulations of ATS gave the same thinning effects. For all thinning treatments, both significant yield reductions and fruit weight increment were noticed during the experimental period. Fruit over color and soluble solids were generally higher and increased significantly with lower crop load, while fruit firmness (Durofel) and total acidity were less affected. In conclusion, different concentrations of sulphur had a moderate thinning effect and are not recommended for use as plum thinners under these conditions. Instead, 1.5% ATS application, (liquid and powder) applied at full bloom, resulted in adequate thinning of ’Jubileum’ plums under cool mesic northern climatic conditions.
Abstract
The forest understory is often associated with rapid rates of carbon and nutrient cycling, but cost-efficient quantification of its biomass remains challenging. We tested a new field technique for understory biomass assessment using an off-the-shelf handheld laser rangefinder. We conducted laser sampling in a pine forest with an understory dominated by invasive woody shrubs, especially Rhamnus frangula L. Laser sampling was conducted using a rangefinder, mounted on a monopod to provide a consistent reference height, and pointed vertically downward. Subsequently, the understory biomass was measured with destructive sampling. A series of metrics derived from the airborne LiDAR literature were evaluated alone and in combination for prediction of understory biomass using best-subsets regression. Resulting fits were good (r2 = 0.85 and 0.84 for the best single metric and best additive metric, respectively, and R2 = 0.93 for the best multivariate model). The results indicate that laser sampling could substantially reduce the need for costly destructive sampling within a double-sampling context.
Authors
Antonio Carlos Ferraz Filho Blas Mola-Yudego José Ramón González-Olabarria José Roberto Soares ScolforoAbstract
This study focuses on the effects of different thinning regimes on clonal Eucalyptus plantations growth. Four different trials, planted in 1999 and located in Bahia and Espírito Santo States, were used. Aside from thinning, initial planting density, and post thinning fertilization application were also evaluated. Before canopy closure, and therefore before excessive competition between trees took place, it was found that stands planted under low densities (667 trees per hectare) presented a lower mortality proportion when compared to stand planted under higher densities (1111 trees per hectare). However, diameter growth prior to thinning operations was not statistically different between these two densities, presenting an overall mean of 4.9 cm/year. After canopy closure and the application of the thinning treatments, it was found that thinning regimes beginning early in the life of the stand and leaving a low number of residual trees presented the highest diameter and height growth. Unthinned treatments and thinning regimes late in the life of the stand (after 5.5 years), leaving a large number of residual trees presented the highest values of basal area production. The choice of the best thinning regime for Eucalyptus clonal material will vary according to the plantation objective.
Abstract
In the Nordic-Baltic region, there has been a growing concern about an increasing occurrence of multiple tops in young stands of Norway spruce. There is however a lack of documentation on the amount of such damages, and the causal agents involved. In two separate studies in SE Norway, we assessed the frequency of multiple tops in young sapling-sized stands, and studied the relationship between the occurrence of multiple tops and lammas growth the previous growing season on the sample trees. Study 1 included 44 planted and 10 naturally regenerated stands, while Study 2 included 68 planted stands with information on seed source. Among sample trees with multiple tops, 57% (Study 1) and 32% (Study 2) had signs of lammas growth the previous autumn, either in the form of an extended leading shoot or swollen bud. Site index as well as sample tree height were positively correlated to the occurrence of both lammas growth and multiple tops in both studies. In Study 1 we show that the probability of lammas growth was significantly higher in planted than in naturally regenerated stands. In Study 2 we show that it was higher in stands planted with seedlings grown from stand-origin seeds compared with improved seed material. Furthermore, the results of both studies show that lammas growth occurs most frequently among the dominant trees in the stand.
Abstract
Ophiostoma spp. (Ophiostomatales, Ascomycota) are well-known fungi associated with bark and ambrosia beetles (Curculionidae: Scolytinae, Platypodinae). Fungi in the Ophiostomatales include serious tree pathogens as well as agents of timber blue-stain. Although these fungi have been extensively studied in the northern hemisphere, very little is known regarding their occurrence on hardwoods in Europe. The aims of the present study were to identify and characterize new Ophiostoma spp. associated with bark and ambrosia beetles infesting hardwoods in Norway and Poland, and to resolve phylogenetic relationships of Ophiostoma spp. related to the Norwegian and Polish isolates, using multigene phylogenetic analyses. Results obtained from five gene regions (ITS, LSU, b-tubulin, calmodulin, translation elongation factor 1-a) revealed four new Ophiostoma spp. These include Ophiostoma hylesinum sp. nov., O. signatum sp. nov., and O. villosum sp. nov. that phylogenetically are positioned within the Ophiostoma ulmi complex. The other new species, Ophiostoma pseudokarelicum sp. nov. reside along with Ophiostoma karelicum in a discrete, well-supported phylogenetic group in Ophiostoma s. stricto. The results of this study clearly show that the diversity and ecology of Ophiostoma spp. on hardwoods in Europe is poorly understood and that further studies are required to enrich our knowledge about these fungi.
Authors
Milan Flach Sebastian Sippel Fabian Gans Ana Bastos Alexander Brenning Markus Reichstein Miguel D. MahechaAbstract
Combined droughts and heatwaves are among those compound extreme events that induce severe impacts on the terrestrial biosphere and human health. A record breaking hot and dry compound event hit western Russia in summer 2010 (Russian heatwave, RHW). Events of this kind are relevant from a hydrometeorological perspective, but are also interesting from a biospheric point of view because of their impacts on ecosystems, e.g., reductions in the terrestrial carbon storage. Integrating both perspectives might facilitate our knowledge about the RHW. We revisit the RHW from both a biospheric and a hydrometeorological perspective. We apply a recently developed multivariate anomaly detection approach to a set of hydrometeorological variables, and then to multiple biospheric variables relevant to describe the RHW. One main finding is that the extreme event identified in the hydrometeorological variables leads to multidirectional responses in biospheric variables, e.g., positive and negative anomalies in gross primary production (GPP). In particular, the region of reduced summer ecosystem production does not match the area identified as extreme in the hydrometeorological variables. The reason is that forest-dominated ecosystems in the higher latitudes respond with unusually high productivity to the RHW. Furthermore, the RHW was preceded by an anomalously warm spring, which leads annually integrated to a partial compensation of 54% (36% in the preceding spring, 18% in summer) of the reduced GPP in southern agriculturally dominated ecosystems. Our results show that an ecosystem-specific and multivariate perspective on extreme events can reveal multiple facets of extreme events by simultaneously integrating several data streams irrespective of impact direction and the variables' domain. Our study exemplifies the need for robust multivariate analytic approaches to detect extreme events in both hydrometeorological conditions and associated biosphere responses to fully characterize the effects of extremes, including possible compensatory effects in space and time.
Authors
Maria Hayes Leen Bastiaens Luisa Gouveia Spyros Gkelis Hanne Skomedal Kari Skjånes Patrick Murray Marco García-Vaquero Muge Isleten Hosoglu John Dodd Despoina Konstantinou Ivo Safarik Graziella Chini Zittelli Vytas Rimkus Victόria del Pino Koenraad Muylaert Christine Edwards Morten Laake Joana Gabriela Laranjeira da Silva Hugo Pereira Joana AbelhoAbstract
No abstract has been registered
Authors
Dainé Hernández-Ochandia Mayra G. Rodríguez-Hernández Ileana Miranda-Cabrera Ernesto Moreno-León Iván Castro-Lizazo Belkis Peteira Delgado-Oramas Ricardo HolgadoAbstract
El objetivo del estudio fue determinar el factor de reproducción (FR) de Meloidogyne incognita (Kofoid y White) Chitwood y el efecto nocivo del nematodo sobre parámetros seleccionados del desarrollo de las plantas de frijol (Phaseolus vulgaris L.) cultivar ‛Cuba Cueto 25-9’. El experimento se realizó, en condiciones semicontroladas, durante 35 días, con niveles poblacionales iniciales (Pi) de 1,5; 2,5 y 5 juveniles de segundo estadio (J2) y huevos del nematodo por gramo de sustrato y plantas de frijol sin nematodos (testigos). Se determinó el Factor de Reproducción (FR) del nematodo y en la planta se evaluaron el Índice de Agallamiento (IA), la altura de las plantas, el diámetro del tallo, el número de hojas, flores y legumbres. Los datos se analizaron y compararon (ANOVA, Duncan (p<0,05)) y la relación entre la altura de las plantas y Pi se determinó a través de análisis de regresión, empleando el Paquete Estadístico SAS, Versión 9.0. El nematodo parasitó y se reprodujo en el cultivar de frijol ‛CC- 25-9’ con valores de FR=1,6; 6,1 y 6,2 y del IA=3; 3,6 y 3,8 en los niveles de Pi=1,5; 2,5 y 5 J2-huevos x gramo de sustrato-1, respectivamente. Con el incremento de la Pi del nematodo se redujo, significativamente, la altura de las plantas del cultivar ‛CC 25-9’ (R2=0.70). El nematodo produjo disminuciones, no significativas, del diámetro del tallo, número de hojas, flores y legumbres; sin embargo, se evidenció una tendencia a que los mayores valores de los parámetros evaluados se presentaron en las plantas sanas. Se discute la importancia de utilizar plantas de la misma familia al momento de evaluar resistencia/susceptibilidad de cultivares frente a Meloidogyne spp.
Authors
Hella Ellen Ahrends Werner Eugster Thomas Gaiser Victor Rueda-Ayala H. Hüging F. Ewert Stefan SiebertAbstract
For highly productive regions such as Germany, the increase of wheat grain yields observed throughout the 20th century is largely attributed to the progress in crop breeding and agronomic management. However, several studies indicate a strong variability of the genetic contribution across locations that further varies with experimental design and variety selection. It is therefore still unclear to which extent management conditions have promoted the realization of the breeding progress in Germany over the last 100+ years. We established a side-by-side cultivation experiment over two seasons(2014/2015 and 2015/2016)including 16 winter wheat varieties released in Germany between 1895 and 2007. The varieties were grown using 24 different long-term fertilization treatments established since 1904 (Dikopshof, Germany). Averaged over all cultivars and treatments mean yields of 6.88 t ha−1 and 5.15 t ha−1were estimated in 2015 and 2016, respectively. A linear mixed effects analysis was performed to study the treatment-specific relation between grain yields and year of variety release. Results indicate a linear increase in grain yields ranging from 0.025 to 0.032 t ha−1 yr−1 (0.304 to 0.387% yr−1 )in plots that were treated with combined synthetic-organic fertilizers without signs of a leveling-off. Yields from low or unfertilized plots do not show a significant progress in yield. Responsiveness of mean yields to fertilizer management increases with year of release and indicates small yield penalties under very low nutrient supply. Results highlight the need to consider the importance of long-term soil fertilization management for the realization of genetic gains and the value of long-term fertilization experiments to study interactions between genetic potential and management.
Abstract
In cold climate regions a significant fraction of annual soil erosion in agricultural land occurs during snowmelt and rain on partially frozen soils. Physically based and spatially distributed soil erosion models have proved to be good tools for understanding the processes occurring at catchment scale during rainfall erosion. However, most existing erosion models do not account for snow in a suitable way. A combination of the UEBGrid snow pack model and the LISEM erosion model was therefore used in this study. The aim was to test and validate this model combination and to assess its utility in relation to quantification and process understanding. Applying this model combination to simulate surface runoff and soil erosion showed that, in principle, it is possible to satisfactorily simulate surface runoff and observed soil erosion patterns during winter. The values for the calibration parameters were similar for the two chosen winter periods when the rainfall and snowmelt episodes occurred. However, the calibration procedure showed that the utility of this combination had several limitations. It is hoped that this study can help to improve existing models and trigger new developments in including snow pack dynamics and soil freezing and thawing in soil erosion models.
Authors
Adam O'Toole Christophe Moni Simon Weldon Anne Schols Monique Carnol Bernard Bosman Daniel RasseAbstract
The application of biochar to soils is a promising technique for increasing soil organic C and offsetting GHG emissions. However, large-scale adoption by farmers will likely require the proof of its utility to improve plant growth and soil quality. In this context, we conducted a four-year field experiment between October 2010 to October 2014 on a fertile silty clay loam Albeluvisol in Norway to assess the impact of biochar on soil physical properties, soil microbial biomass, and oat and barley yield. The following treatments were included: Control (soil), miscanthus biochar 8 t C ha1 (BC8), miscanthus straw feedstock 8 t C ha1 (MC8), and miscanthus biochar 25 t C ha1 (BC25). Average volumetric water content at field capacity was significantly higher in BC25 when compared to the control due to changes in BD and total porosity. The biochar amendment had no effect on soil aggregate (2–6 mm) stability, pore size distribution, penetration resistance, soil microbial biomass C and N, and basal respiration. Biochar did not alter crop yields of oat and barley during the four growing seasons. In order to realize biochar’s climate mitigation potential, we suggest future research and development efforts should focus on improving the agronomic utility of biochar in engineered fertilizer and soil amendment products.
Authors
Klaus Mittenzwei Wolfgang BritzAbstract
Norway maintains a complex system of activity or type specific coupled paymentswhich account for a large share of farm income. Most of the payment rates arenegatively related to farm size and are higher in remote areas compared to centralregions. We present and use a newly developed recursive-dynamic multi-commoditymodel (Agrispace) with CES production functions depicting regional farm clustersderived from the full farm population. Using this model, we simulate impacts ofcurrent and alternative subsidy policies on production, prices, input use, incomeand farm structural change. Mapping cluster results to each farm along with beha-vioural rules allows estimation of individual profits and farm exits. Our results indi-cate that, in the short run, the current policy regime seems to support the policyobjective of maintaining a variety of farms in all parts of Norway. In the long run,farm structural change is less affected by a policy reform that leaves total supportlevels unchanged.
Authors
Manuel J. Steinbauer John-Arvid Grytnes Gerald Jurasinski Aino Kulonen Jonathan Lenoir Harald Pauli Christian Rixen Manuela Winkler Manfred Bardy-Durchhalter Elena Barni Anne D. Bjorkman Frank T. Breiner Sarah Burg Patryk Czortek Melissa A. Dawes Anna Delimat Stefan Dullinger Brigitta Erschbamer Vivian Astrup Felde Olatz Fernández-Arberas Kjetil Farsund Fossheim Daniel Gómez-García Damien Georges Erlend T. Grindrud Sylvia Haider Siri Vatsø Haugum Hanne Henriksen Maria J. Herreros Bogdan Jaroszewicz Francesca Orinda Holl Jaroszynska Robert Kanka Jutta Kapfer Kari Klanderud Ingolf Kühn Andrea Lamprecht Magali Matteodo Umberto Morra di Cella Signe Normand Arvid Odland Siri Lie Olsen Sara Palacio Martina Petey Veronika Piscová Blazena Sedlakova Klaus Steinbauer Veronika Stöckli Jens-Christian Svenning Guido Teppa Jean-Paul Theurillat Pascal Vittoz Sarah J. Woodin Niklaus E. Zimmermann Sonja WipfAbstract
Globally accelerating trends in societal development and human environmental impacts since the mid-twentieth century1–7 are known as the Great Acceleration and have been discussed as a key indicator of the onset of the Anthropocene epoch6 . While reports on ecological responses (for example, changes in species range or local extinctions) to the Great Acceleration are multiplying8,9 , it is unknown whether such biotic responses are undergoing a similar acceleration over time. This knowledge gap stems from the limited availability of time series data on biodiversity changes across large temporal and geographical extents. Here we use a dataset of repeated plant surveys from 302 mountain summits across Europe, spanning 145 years of observation, to assess the temporal trajectory of mountain biodiversity changes as a globally coherent imprint of the Anthropocene. We find a continent-wide acceleration in the rate of increase in plant species richness, with five times as much species enrichment between 2007 and 2016 as fifty years ago, between 1957 and 1966. This acceleration is strikingly synchronized with accelerated global warming and is not linked to alternative global change drivers. The accelerating increases in species richness on mountain summits across this broad spatial extent demonstrate that acceleration in climate-induced biotic change is occurring even in remote places on Earth, with potentially far-ranging consequences not only for biodiversity, but also for ecosystem functioning and services.
Authors
Jian Liu Peter J. A. Kleinman Helena Aronsson Don Flaten Richard W. McDowell Marianne Bechmann Douglas B. Beegle Timothy P. Robinson Ray B. Bryant Hongbin Liu Andrew N. Sharpley Tamie L. VeithAbstract
Winter manure application elevates nutrient losses and impairment of water quality as compared to manure applications in other seasons. In conjunction with reviewing global distribution of animal densities, we reviewed worldwide mandatory regulations and voluntary guidelines on efforts to reduce off-site nutrient losses associated with winter manure applications. Most of the developed countries implement regulations or guidelines to restrict winter manure application, which range from a regulative ban to guidelines based upon weather and field management conditions. In contrast, developing countries lack such official directives, despite an increasing animal production industry and concern over water quality. An analysis of five case studies reveals that directives are derived from a common rationale to reduce off-site manure nutrient losses, but they are also affected by local socioeconomic and biophysical considerations. Successful programs combine site-specific management strategies along with expansion of manure storage to offer farmers greater flexibility in winter manure management.
Abstract
Finding efficient ways to decrease wood decay caused by fungi is an important issue in the timber construction. A possible way to avoid wood decay by fungi is by reducing the water content of wood, since water is a primary condition for fungal growth. Bulking of the wood cell wall by chemical reagents occupies the space where water normally occurs. This also improves the dimensional stability of the modified wood. The aim of the work was to react non-toxic reagents using a Maillard type of reaction in the wood cell wall. Wood was soaked in different aqueous solutions with a primary amine and a sugar as the main constituents. The wood was thereafter cured in an oven at 120°C. The preliminary results showed that the use of the Maillard reaction for wood modification is a promising method and is worth further research.
Authors
Katja Hannele Karppinen Pinja Tegelberg Hely Häggman Laura JaakolaAbstract
Ripening of non-climacteric bilberry (Vaccinium myrtillus L.) fruit is characterized by a high accumulation of health-beneficial anthocyanins. Plant hormone abscisic acid (ABA) and sucrose have been shown to be among the central signaling molecules coordinating non-climacteric fruit ripening and anthocyanin accumulation in some fruits such as strawberry. Our earlier studies have demonstrated an elevation in endogenous ABA level in bilberry fruit at the onset of ripening indicating a role for ABA in the regulation of bilberry fruit ripening. In the present study, we show that the treatment of unripe green bilberry fruits with exogenous ABA significantly promotes anthocyanin biosynthesis and accumulation both in fruits attached and detached to the plant. In addition, ABA biosynthesis inhibitor, fluridone, delayed anthocyanin accumulation in bilberries. Exogenous ABA also induced the expression of several genes involved in cell wall modification in ripening bilberry fruits. Furthermore, silencing of VmNCED1, the key gene in ABA biosynthesis, was accompanied by the down-regulation in the expression of key anthocyanin biosynthetic genes. In contrast, the treatment of unripe green bilberry fruits with exogenous sucrose or glucose did not lead to an enhancement in the anthocyanin accumulation neither in fruits attached to plant nor in post-harvest fruits. Moreover, sugars failed to induce the expression of genes associated in anthocyanin biosynthesis or ABA biosynthesis while could elevate expression of some genes associated with cell wall modification in post-harvest bilberry fruits. Our results demonstrate that ABA plays a major role in the regulation of ripening-related processes such as anthocyanin biosynthesis and cell wall modification in bilberry fruit, whereas sugars seem to have minor regulatory roles in the processes. The results indicate that the regulation of bilberry fruit ripening differs from strawberry that is currently considered as a model of nonclimacteric fruit ripening. In this study, we also identified transcription factors, which expression was enhanced by ABA, as potential regulators of ABA-mediated bilberry fruit ripening processes.
Authors
Greeley Beck Emil Engelund Thybring Lisbeth Garbrecht ThygesenAbstract
Earlywood samples of unmodified and acetylated radiata pine were exposed to the brown-rot fungus Rhodonia placenta for 1, 2, 3 and 4 weeks for unmodified samples and 10, 16, 24 and 28 weeks for acetylated samples. Longer incubation periods were used for acetylated samples based on the hypothesis that given enough time under favourable conditions the fungus would eventually degrade the wood. After exposure, samples were weighed and chemically characterized by ATR-FTIR analysis, acetyl content by saponification, and hydroxyl (OH) accessibility by deuterium exchange. Longer incubation times for acetylated samples led to comparable levels of mass loss between unmodified and acetylated wood. Initial brown-rot decay in acetylated wood exhibited a different trend compared to unmodified wood, with an increased OH accessibility and a significant reduction in acetyl content. This was followed by a stable, low OH accessibility and plateau in acetyl content above 10% mass loss in acetylated wood. In unmodified wood, the OH accessibility was nearly constant throughout decay, while the initially low acetyl content decreased linearly with mass loss. ATR-FTIR analysis confirmed the differences in acetyl removal between unmodified and acetylated wood. Wood-water relations before and after brown-rot decay were determined with low-field nuclear magnetic resonance (LFNMR) relaxometry on water saturated samples. For the decayed acetylated wood, the behaviour of the water corresponded well with de-acetylation observed by chemical characterization. The results show that after removal of acetyl groups, degradation of acetylated wood by R. placenta occurred at a similar rate to that of unmodified wood.
Authors
Carrie Joy Andrew Rune Halvorsen Einar Heegaard Thomas W. Kuyper Jacob Heilmann-Clausen Irmgard Krisai-Greilhuber Claus Bässler Simon Egli Alan C. Gange Klaus Høiland Paul M. Kirk Beatrice Senn-Irlet Lynne Boddy Ulf Büntgen Håvard KauserudAbstract
Aim:Macroecological scales of species compositional trends are well documentedfor a variety of plant and animal groups, but remain sparse for fungi, despite theirecological importance in carbon and nutrient cycling. It is, thus, essential to under-stand the composition of fungal assemblages across broad geographical scales andthe underlying drivers. Our overall aim was to describe these patterns for fungiacross two nutritional modes (saprotrophic and ectomycorrhizal). Furthermore, weaimed to elucidate the temporal component of fruiting patterns and to relate theseto soil carbon and nitrogen deposition. Location:Central and Northern Europe.Methods:A total of 4.9 million fungal fruit body observations throughout Europe,collected between 1970 and 2010, were analysed to determine the two main envi-ronmental and geographical gradients structuring fungal assemblages for two mainnutritional modes, saprotrophic and ectomycorrhizal fungi. Results:Two main gradients explaining the geography of compositional patternswere identified, for each nutritional mode. Mean annual temperature (and relatedcollinear, seasonal measures) correlated most strongly with the first gradient forboth nutritional modes. Soil organic carbon was the highest correlate of the second compositional gradient for ectomycorrhizal fungi, suspected as an indicator of vege-tation- and pH-related covariates. In contrast, nitrogen deposition constituted asecond gradient for saprotrophic fungi, likely a proxy for anthropogenic pollution.Compositional gradients and environmental conditions correlated similarly whenthe data were divided into two time intervals of 1970–1990 and 1991–2010.Evidence of compositional temporal change was highest with increasing elevationand latitude. Main conclusions:Fungal assemblage patterns demonstrate clear biogeographicalpatterns that relate the nutritional modes to their main environmental correlates oftemperature, soil organic carbon and nitrogen deposition. With respect to globalchange impacts, the highest rates of compositional change by time suggest targetinghigher latitudes and elevations for a better understanding of fungal dynamics. We,finally, suggest further examination of the ranges and dispersal abilities of fungi tobetter assess responses to global change.
Abstract
Acetylation of wood can provide protection against wood deteriorating fungi, but the exact degradation me- chanism remains unclear. The aim of this study was to determine the effect of acetylation of Pinus radiata wood (weight percent gain 13, 17 and 21%) on the expression of genes involved in decay by brown-rot fungus Rhodonia placenta. Gene expression analysis using qRT-PCR captured incipient to advanced decay stages. As expected the initiation of decay was delayed as a result the degree of acetylation. However, once decay was established, the rate of degradation in acetylated samples was similar to that of unmodi fied wood. This suggests a delay in decay rather than an absolute protection threshold at higher acetylation levels. In accordance with previous studies, the oxidative system of R. placenta was more active in wood with higher degrees of acetylation and expression of cellulose active enzymes was delayed for acetylated samples compared to untreated samples. The reason for the delay in the latter might be because of the slower diffusion rate in acetylated wood or that partially acetylated cellobiose may be less effective in triggering production of saccharification enzymes. Enzymes involved in hemicellulose and pectin degradation have previously not been focused on in studies of degradation of acetylated wood. Surprisingly, CE16 carbohydrate esterase, assumed to be involved in deace- tylation of carbohydrates, was expressed significantly more in untreated samples compared to highly acetylated samples. We hypothesise that this enzyme might be regulated through a negative feedback system, where acetic acid supresses the expression. The up-regulation of two expansin genes in acetylated samples suggests that their function, to loosen the cell wall, is needed more in acetylated wood due the physical bulking of the cell wall. In this study, we demonstrate that acetylation affects the expression of specific target genes not previously re- ported, resulting in delayed initiation of decay. Thus, targeting these degradation mechanisms can contribute to improving wood protection systems.
Abstract
The potential impact of silver nanoparticles (Ag NPs) on aquatic organisms is to a large extent determined by theirbioavailability through different routes of exposure. In the present study juvenile Atlantic salmon (Salmo salar) were exposed todifferent sources of radiolabeled Ag (radiolabeled110mAg NPs and110mAgNO3). After 48 h of waterborne exposure to 3mg/Lcitrate stabilized110mAg NPs or110mAgNO3, or a dietary exposure to 0.6mg Ag/kg fish (given as citrate stabilized or uncoated110mAg NPs, or110mAgNO3), Ag had been taken up in fish regardless of route of exposure or source of Ag (Ag NPs or AgNO3).Waterborne exposure led to high Ag concentrations on the gills, and dietary exposure led to high concentrations in thegastrointestinal tract. Silver distribution to the target organs was similar for both dietary and waterborne exposure, with the liveras the main target organ. The accumulation level of Ag was 2 to 3 times higher for AgNO3than for Ag NPs when exposure wasthrough water, whereas no significant differences were seen after dietary exposure. The transfer (Bq/g liver/g food or water)from exposure through water was 4 orders of magnitude higher than from feed using the smallest, citrate-stabilized Ag NPs(4 nm). The smallest NPs had a 5 times higher bioavailability in food compared with the larger and uncoated Ag NPs (20 nm).Despite the relatively low transfer of Ag from diet to fish, the short lifetime of Ag NPs in water and their transfer to sediment,feed, or sediment-dwelling food sources such as larvae and worms could make diet a significant long-term exposure route.
Abstract
Large terrestrial carnivores can sometimes display strong family bonds affecting the spatial distribution of related individuals. We studied the spatial genetic relatedness and family structure of female Eurasian lynx, continuously distributed in southern Finland. We hypothesized that closely related females form matrilineal assemblages, clustering together with relatives living in the neighboring areas. We evaluated this hypothesis using tissue samples of 133 legally harvested female lynx (from year 2007 to 2015), genotyped with 23 microsatellite markers, and tested for possible spatial genetic family structure using a combination of Bayesian clustering, spatial autocor ‐ relation, and forensic genetic parentage analysis. The study population had three potential family genetic clusters, with a high degree of admixture and geographic overlap, and showed a weak but significant negative relationship between pairwise genetic and geographic distance. Moreover, parentage analysis indicated that 64% of the females had one or more close relatives (sister, mother, or daughter) within the study population. Individuals identified as close kin consistently assigned to the same putative family genetic cluster. They also were sampled closer geographically than females on average, although variation was large. Our results support the possibility that Eurasian lynx forms matrilineal assemblages, and comparisons with males are now required to further assess this hypothesis.
Authors
Pablo P. Leal Catriona L. Hurd Sylvia G. Sander Evelyn Armstrong Pamela A. Fernández Tim J. Suhrhoff Michael RoledaAbstract
Ocean warming (OW), ocean acidification (OA) and their interaction with local drivers, e.g., copper pollution, may negatively affect macroalgae and their microscopic life stages. We evaluated meiospore development of the kelps Macrocystis pyrifera and Undaria pinnatifida exposed to a factorial combination of current and 2100-predicted temperature (12 and 16 °C, respectively), pH (8.16 and 7.65, respectively), and two copper levels (no-added-copper and species-specific germination Cu- EC50). Meiospore germination for both species declined by 5–18% under OA and ambient temperature/ OA conditions, irrespective of copper exposure. Germling growth rate declined by >40%·day−1, and gametophyte development was inhibited under Cu-EC50 exposure, compared to the no-added-copper treatment, irrespective of pH and temperature. Following the removal of copper and 9-day recovery under respective pH and temperature treatments, germling growth rates increased by 8–18%·day−1. The exception was U. pinnatifida under OW/OA, where growth rate remained at 10%·day−1 before and after copper exposure. Copper-binding ligand concentrations were higher in copper-exposed cultures of both species, suggesting that ligands may act as a defence mechanism of kelp early life stages against copper toxicity. Our study demonstrated that copper pollution is more important than global climate drivers in controlling meiospore development in kelps as it disrupts the completion of their life cycle.
Authors
Pauline Bristiel Lauren Gillespie Liv Østrem Jennifer Balachowski Cyrille Violle Florence VolaireAbstract
1. A core tenet of functional ecology is that the vast phenotypic diversity observed in the plant kingdom could be partly generated by a trade-off between the ability of plants to grow quickly and acquire resources in rich environments vs. the ability to conserve resources and avoid mortality under stress. However, experimental demonstrations remain scarce and potentially blurred by phylogenetic constraints in cross-species analyses. Here, we experimentally decoupled growth potential and stress survival by applying an off-season stress on contrasting populations of the perennial grass Dactylis glomerata exhibiting a range of seasonal dormancy.2. Seventeen populations of D. glomerata, originating from a latitudinal gradient from Norway to Morocco, were subjected to three types of dehydration stress: winter frost in Norway, and summer drought and early spring (off-season) drought stress in the south of France. Growth rate and two leaf traits (leaf width and leaf dry matter content) suspected to be involved in the adaptation to dehydration stress were monitored under optimal conditions. We quantified plant dehydration survival as the amount of plant recovery after a severe stress.3. Nordic populations were found to be winter-dormant. Winter- and summer-dor-mant populations better survived frost and summer drought, respectively. However, no trade-off between growth potential and dehydration survival was detected in non-dormant plants in early spring when dehydration occurred unsea-sonably for all populations. Furthermore, Mediterranean populations better survived an early spring drought.4. Our results highlight the importance of assessing plant growth potential as a re-sponse to seasonal environmental cues. They suggest that growth potential and stress survival trade off when plants exhibit seasonal dormancy but can be func-tionally independent at other seasons. Consequently, the growth–stress survival relationship could be better described as a dynamic linkage rather than a constant and general trade-off. Moreover, leaf trait values, such as thinner and more ligni-fied leaves reflecting drought adaptation, may have contributed to the improved drought-stress survival without resulting in a cost to growth. 5. Further exploration of the growth–stress survival relationship should permit deci-phering the suite of plant traits and trait covariations involved in plants’ responses to increasing stress.
Abstract
We investigated the ability of the fungal entomopathogen Beauveria bassiana strain GHA to endophytically colonize sugarcane (Saccharum officinarum) and its impact on plant growth. We used foliar spray, stem injection, and soil drench inoculation methods. All three inoculation methods resulted in B. bassiana colonizing sugarcane tissues. Extent of fungal colonization differed significantly with inoculation method (χ2 = 20.112, d. f. = 2, p < 0.001), and stem injection showed the highest colonization level followed by foliar spray and root drench. Extent of fungal colonization differed significantly with plant part (χ2 = 33.072, d. f. = 5, p < 0.001); stem injection resulted in B. bassiana colonization of the stem and to some extent leaves; foliar spray resulted in colonization of leaves and to some extent, the stem; and soil drench resulted in colonization of roots and to some extent the stem. Irrespective of inoculation method, B. bassiana colonization was 2.8 times lower at 14–16 d post inoculation (DPI) than at 7–10 DPI (p = 0.020). Spraying leaves and drenching the soil with B. bassiana significantly (p = 0.01) enhanced numbers of sett roots. This study demonstrates for the first time that B. bassiana can endophytically colonize sugarcane plants and enhance the root sett and it provides a starting point for exploring the use of this fungus as an endophyte in management of sugarcane pests.
Authors
Robert Jankowiak Agnieszka Ostafińska Truls Aas Halvor Solheim Piotr Bilański Riikka Linnakoski Georg HausnerAbstract
Species of Leptographium are characterized by mononematous or synnematous conidiophores and are commonly associated with different arthropods. Some of them also produce a sexual state characterised by globose ascomata with elongated necks. Compared to investigations on coniferous trees, the occurrence of Leptographium species on hardwood trees has been poorly studied in Europe. During a survey of ophiostomatoid fungi on various hardwood tree species in Norway and Poland, three unusual species, which fit in the broader morphological description of Leptographium spp., were found in association with Trypodendron domesticum, Trypodendron signatum and Dryocoetes alni, and from wounds on a variety of hardwoods. Phylogenetic analyses of sequence data for six different loci (ITS1–5.8 S–ITS2, ITS2-LSU, ACT, b-tubulin, CAL, and TEF-1a) showed that these Leptographium species are phylogenetically closely related to the species of the Grosmannia olivacea complex. The first species forms a well-supported lineage that includes Ophiostoma brevicolle, while the two other new taxa resided in a separate lineage; possibly affiliated with Grosmannia francke-grosmanniae. All the new species produce perithecia with necks terminating in ostiolar hyphae and orange-section shaped ascospores with cucullate, gelatinous sheaths. These species also produce dark olivaceous mononematous asexual states in culture. In addition, two of the newly described species have a second type of conidiophore with a short and non-pigmented stipe. The new Leptographium species can be easily distinguished from each other by their appearance and growth in culture. Based on novel morphological characters and distinct DNA sequences, these fungi were recognised as new taxa for which the names Leptographium tardum sp. nov., Leptographium vulnerum sp. nov., and Leptographium flavum sp. nov. are provided.
Authors
Haakon Helland A Leufvén Gunnar Bengtsson Hanne Larsen Elin Merete Nicolaisen Mette Thomsen Arne Hermansen Anne-Berit Wold Liv BergeAbstract
Preliminary results on aroma profiles (GC-MS) related to storage conditions (temperature, time and packaging atmosphere) are presented. The vegetables used in the experiments were rutabaga, carrot and turnip, which were peeled and cut before packaging, and stored at two different temperatures. O2 and CO2 concentrations in the packaging atmosphere were measured during the storage period to calculate the respiration rates of the produce. Cubed carrot showed a higher respiration rate than cubed turnip and rutabaga. Samples for analysis of volatiles were taken after 0 and 7 or 10 days. This type of analysis could be used as a complement to sensory analysis.
Authors
Andrew D. Armitage Erik Lysøe Charlotte F. Nellist Laura A. Lewis Liliana M. Cano Richard J. Harrison May Bente BrurbergAbstract
The oomycete pathogen Phytophthora cactorum causes crown rot, a major disease of cultivated strawberry. We report the draft genome of P. cactorum isolate 10300, isolated from symptomatic Fragaria x ananassa tissue. Our analysis revealed that there are a large number of genes encoding putative secreted effectors in the genome, including nearly 200 RxLR domain containing effectors, 77 Crinklers (CRN) grouped into 38 families, and numerous apoplastic effectors, such as phytotoxins (PcF proteins) and necrosis inducing proteins. As in other Phytophthora species, the genomic environment of many RxLR and CRN genes differed from core eukaryotic genes, a hallmark of the two-speed genome. We found genes homologous to known Phytophthora infestans avirulence genes including Avr1, Avr3b, Avr4, Avrblb1 and AvrSmira2 indicating effector sequence conservation between Phytophthora species of clade 1a and clade 1c. The reported P. cactorum genome sequence and associated annotations represent a comprehensive resource for avirulence gene discovery in other Phytophthora species from clade 1 and, will facilitate effector informed breeding strategies in other crops.
Authors
Jürgen Dengler Viktoria Wagner Iwona Dembicz Itziar García-Mijangos Alireza Naqinezhad Steffen Boch Alessandro Chiarucci Timo Conradi Goffredo Filibeck Riccardo Guarino Monika Janišová Manuel J. Steinbauer Svetlana Aćić Alicia T.R. Acosta Munemitsu Akasaka Marc-Andre Allers Iva Apostolova Irena Axmanová Branko Bakan Alina Baranova Manfred Bardy-Durchhalter Sándor Bartha Esther Baumann Thomas Becker Ute Becker Elena Belonovskaya Karin Bengtsson José Luis Benito Alonso Asun Berastegi Ariel Bergamini Ilaria Bonini Hans Henrik Bruun Vasyl Budzhak Alvaro Bueno Juan Antonio Campos Laura Cancellieri Marta Carboni Cristina Chocarro Luisa Conti Marta Czarniecka-Wiera Pieter De Frenne Balázs Deák Yakiv P. Didukh Martin Diekmann Christian Dolnik Cecilia Duprè Klaus Ecker Nikolai Ermakov Brigitta Erschbamer Adrián Escudero Javier Etayo Zuzana Fajmonová Vivian Astrup Felde Maria Rosa Fernández Calzado Manfred Finckh Georgios Fotiadis Mariano Fracchiolla Anna Ganeva Daniel García-Magro Rosario G. Gavilán Markus Germany Itamar Giladi François Gillet Gian Pietro Giusso del Galdo Jose M. González John-Arvid Grytnes Michal Hájek Petra Hájková Aveliina Helm Mercedes Herrera Eva Hettenbergerová Carsten Hobohm Elisabeth M. Hüllbusch Nele Ingerpuu Ute Jandt Florian Jeltsch Kai Jensen Anke Jentsch Michael Jeschke Borja Jiménez-Alfaro Zygmunt Kacki Kaoru Kakinuma Jutta Kapfer Ali Kavgaci András Kelemen Kathrin Kiehl Asuka Koyama Tomoyo F. Koyanagi Łukasz Kozub Anna Kuzemko Magni Olsen Kyrkjeeide Sara Landi Nancy Langer Lorenzo Lastrucci Lorenzo Lazzaro Chiara Lelli Jan Lepš Swantje Löbel Arantzazu L. Luzuriaga Simona Maccherini Martin Magnes Marek Malicki Corrado Marcenó Constantin Mardari Leslie Mauchamp Felix May Ottar Michelsen Joaquín Molero Mesa Zsolt Molnár Ivan Y. Moysiyenko Yuko K. Nakaga Rayna Natcheva Jalil Noroozi Robin J. Pakeman Salza Palpurina Meelis Pärtel Ricarda Pätsch Harald Pauli Hristo Pedashenko Robert K. Peet Remigiusz Pielech Nataša Pipenbaher Chrisoula Pirini Zuzana Plesková Mariya A. Polyakova Honor C. Prentice Jennifer Reinecke Triin Reitalu Maria Pilar Rodríguez-Rojo Jan Roleček Vladimir Ronkin Leonardo Rosati Ejvind Rosén Eszter Ruprecht Solvita Rusina Marko Sabovljević Ana María Sánchez Galina Savchenko Oliver Schuhmacher Sonja Škornik Marta Gaia Sperandii Monika Staniaszek-Kik Zora Stevanović-Dajić Marin Stock Sigrid Suchrow Laura M. E. Sutcliffe Grzegorz Swacha Martin Sykes Anna Szabó Amir Talebi Cătălin Tănase Massimo Terzi Csaba Tölgyesi Marta Torca Péter Török Béla Tóthmérész Nadezda Tsarevskaya Ioannis Tsiripidis Rossen Tzonev Atushi Ushimaru Orsolya Valkó Eddy van der Maarel Thomas Vanneste Iuliia Vashenyak Kiril Vassilev Daniele Viciani Luis Villar Risto Virtanen Ivana Vitasović Kosić Yun Wang Frank Weiser Julia Went Karsten Wesche Hannah White Manuela Winkler Piotr T. Zaniewski Hui Zhang Yaron Ziv Sergey Znamenskiy Idoia BiurrunAbstract
GrassPlot is a collaborative vegetation-plot database organised by the Eurasian Dry Grassland Group (EDGG) and listed in the Global Index of Vegetation-Plot Databases (GIVD ID EU-00-003). GrassPlot collects plot records (relevés) from grasslands and other open habitats of the Palaearctic biogeographic realm. It focuses on precisely delimited plots of eight standard grain sizes (0.0001; 0.001; ... 1,000 m²) and on nested-plot series with at least four different grain sizes. The usage of GrassPlot is regulated through Bylaws that intend to balance the interests of data contributors and data users. The current version (v. 1.00) contains data for approximately 170,000 plots of different sizes and 2,800 nested-plot series. The key components are richness data and metadata. However, most included datasets also encompass compositional data. About 14,000 plots have near-complete records of terricolous bryophytes and lichens in addition to vascular plants. At present, GrassPlot contains data from 36 countries throughout the Palaearctic, spread across elevational gradients and major grassland types. GrassPlot with its multi-scale and multi-taxon focus complements the larger international vegetationplot databases, such as the European Vegetation Archive (EVA) and the global database “sPlot”. Its main aim is to facilitate studies on the scale- and taxon-dependency of biodiversity patterns and drivers along macroecological gradients. GrassPlot is a dynamic database and will expand through new data collection coordinated by the elected Governing Board. We invite researchers with suitable data to join GrassPlot. Researchers with project ideas addressable with GrassPlot data are welcome to submit proposals to the Governing Board.
Authors
Markus Berger Stephanie Eisner Ruud van der Ent Martina Flörke Andreas Link Joseph Poligkeit Vanessa Bach Matthias FinkbeinerAbstract
Due to the increasing relevance of analyzing water consumption along product life cycles, the water accounting and vulnerability evaluation model (WAVE) has been updated and methodologically enhanced. Recent data from the atmospheric moisture tracking model WAM2-layers is used to update the basin internal evaporation recycling (BIER) ratio, which denotes atmospheric moisture recycling within drainage basins. Potential local impacts resulting from water consumption are quantified by means of the water deprivation index (WDI). Based on the hydrological model WaterGAP3, WDI is updated and methodologically refined to express a basin’s vulnerability to freshwater deprivation resulting from the relative scarcity and absolute shortage of water. Compared to the predecessor version, BIER and WDI are provided on an increased spatial and temporal (monthly) resolution. Differences compared to annual averages are relevant in semiarid and arid basins characterized by a high seasonal variation of water consumption and availability. In order to support applicability in water footprinting and life cycle assessment, BIER and WDI are combined to an integrated WAVE+ factor, which is provided on different temporal and spatial resolutions. The applicability of the WAVE+ method is proven in a case study on sugar cane, and results are compared to those obtained by other impact assessment methods.
Abstract
Forest management affects the distribution of tree species and the age class of a forest, shaping its overall structure and functioning and in turn the surface–atmosphere exchanges of mass, energy, and momentum. In order to attribute climate effects to anthropogenic activities like forest management, good accounts of forest structure are necessary. Here, using Fennoscandia as a case study, we make use of Fennoscandic National Forest Inventory (NFI) data to systematically classify forest cover into groups of similar aboveground forest structure. An enhanced forest classification scheme and related lookup table (LUT) of key forest structural attributes (i.e., maximum growing season leaf area index (LAImax), basal-area-weighted mean tree height, tree crown length, and total stem volume) was developed, and the classification was applied for multisource NFI (MSNFI) maps from Norway, Sweden, and Finland. To provide a complete surface representation, our product was integrated with the European Space Agency Climate Change Initiative Land Cover (ESA CCI LC) map of present day land cover (v.2.0.7). Comparison of the ESA LC and our enhanced LC products (https://doi.org/10.21350/7zZEy5w3) showed that forest extent notably (κ = 0.55, accuracy 0.64) differed between the two products. To demonstrate the potential of our enhanced LC product to improve the description of the maximum growing season LAI (LAImax) of managed forests in Fennoscandia, we compared our LAImax map with reference LAImax maps created using the ESA LC product (and related cross-walking table) and PFT-dependent LAImax values used in three leading land models. Comparison of the LAImax maps showed that our product provides a spatially more realistic description of LAImax in managed Fennoscandian forests compared to reference maps. This study presents an approach to account for the transient nature of forest structural attributes due to human intervention in different land models.
Abstract
Although pasture is low-cost feed, many farmers find it difficult to maintain high milk yield when using pasturefor high-yielding dairy cows in automatically milked herds. Therefore, a seven-week experiment with 40 cows inearly to mid-lactation was performed to evaluate a management model for including pasture in the diet withoutjeopardizing milk production. Within a part-time grazing system with morning and evening outdoor access, wecompared a group with ad libitum grass silage indoors combined with access to a small grass-covered permanentpaddock for exercise and recreation (group EX) with a group offered production pasture at a high allowance percow and day combined with restricted grass silage allowance at night (group PROD). Both groups had the sameoutdoor access times and the same concentrate allowance based on pre-experimental milk yield. Milk yield andmilking frequency were recorded daily in the automatic milking unit. Milk recordings and samplings for de-termination of milk composition took place weekly and outdoor behaviour of cows was recorded during pastureaccess hours on six observation days, evenly distributed over the experimental period. During the experiment,average metabolisable energy concentration was higher in the grass silage offered both groups than in pastureherbage. However, our results showed no significant difference in daily milk yield between treatments.Furthermore, no signifi cant differences between treatments were found in energy-corrected milk, milk fatproduction, or body weight change. Milk protein production was, however, significantly higher in group PROD.In early lactation, no difference in milking frequency was observed between treatments while for cows in mid- tolate lactation, milking frequency was significantly higher in group EX than group PROD. Over the entire ex-periment, group EX cows spent significantly less time outdoors than group PROD. In conclusion, offering highyielding dairy cows in automatic milking systems high-quality pasture at a high allowance for a few hours inmorning and afternoon appears to be an interesting alternative to exercise paddock with full indoor feeding, as itcan reduce costs for supplementary silage, facilitate natural behaviour, and encourage cows to spend more timeoutdoors, while maintaining milk production at a level comparable to that of full indoor feeding.
Authors
Liv Østrem Marcin Rapacz Arild Larsen Petter Marum Odd Arne RognliAbstract
In a ×Festulolium population (FuRs0357) of parental origin Lolium perenne × Festuca pratensis, selection of freezing tolerance by freezing tests on whole plants (FT) and chlorophyll a (Chl-a) fluorimetry on frozen detached leaves (CF) was assessed in high and low directions during two cycles of selection. The original population went through two cycles of random mating. All selections and non-selected intercrossed generations of the original population were established in field trials at a coastal site and a continental site in Norway. At the coastal site, analyses of Chl-a fluorimetry parameters and leaf growth on individual plants in autumn and winter hardiness observed in field plots in spring showed that the first-generation selections for high freezing tolerance were associated with winter hardiness and early growth cessation. The second-generation FT-selections for high freezing tolerance were also associated with winter hardiness, whereas the CF-high selections diverged toward high photosynthetic activity. Both low selections were correlated with high photosynthetic activity. There were smaller variations between generations in unselected generations of the original population. Low accumulated leaf growth and early growth cessation were observed in the secondgeneration FT-selection for high freezing tolerance, whereas high normalized difference vegetation index (NDVI) were seen in Chl-a selections. Both selection methods distinguished diverging selections with significantly different high and low freezing tolerance, but selection efficiency was comparable only for the first selection cycle. Moreover, due to mixed ploidy level in the original population, selection by FT and CF generated diploid and tetraploid plants, respectively, which intensified the response of selection, particularly in the diploid selections. Total dry matter yield (DMY) (mean of three annual cuts for 3 years) of the FT-high selections was lower than for the CF-selections. At coastal sites, selection intensity using freezing tests on whole plants should be adapted to actual climate conditions, to obtain genotypes that balance photosynthetic activity during autumn and good winter hardiness, making them persistent and high yielding.
Authors
Rachel Cassidy Philip Jordan Marianne Bechmann Brian Kronvang Katarina Kyllmar Mairead ShoreAbstract
Achieving an operational compromise between spatial coverage and temporal resolution in national scale river water quality monitoring is a major challenge for regulatory authorities, particularly where chemical concentrations are hydrologically dependent. The efficacy of flow-weighted composite sampling (FWCS) approaches for total phosphorus (TP) sampling (n = 26–52 analysed samples per year), previously applied in monitoring programmes in Norway, Sweden and Denmark, and which account for low to high flow discharges, was assessed by repeated simulated sampling on high resolution TP data. These data were collected in three research catchments in Ireland over the period 2010–13 covering a base-flow index range of 0.38 to 0.69. Comparisons of load estimates were also made with discrete (set time interval) daily and sub-daily sampling approaches (n = 365 to >1200 analysed samples per year). For all years and all sites a proxy of the Norwegian sampling approach, which is based on re-forecasting discharge for each 2-week deployment, proved most stable (median TP load estimates of 87–98%). Danish and Swedish approaches, using long-term flow records to set a flow constant, were only slightly less effective (median load estimates of 64–102% and 80–96%, respectively). Though TP load estimates over repeated iterations were more accurate using the discrete approaches, particularly the 24/7 approach (one sample every 7 h in a 24 bottle sampler - median % load estimates of 93–100%), composite load estimates were more stable, due to the integration of multiple small samples (n = 100–588) over a deployment.
Abstract
Recent technological breakthroughs of optical sensors and analysers have enabled matching the water quality measurement interval to the time scales of stream flow changes and led to an improved understanding of spatially and temporally heterogeneous sources and delivery pathways for many solutes and particulates. This new ability to match the chemograph with the hydrograph has promoted renewed interest in the concentration-discharge (c-q) relationship and its value in characterizing catchment storage, time lags and legacy effects for both weathering products and anthropogenic pollutants. In this paper we evaluated the stream c-q relationships for a number of water quality determinands (phosphorus, suspended sediments, nitrogen) in intensively managed agricultural catchments based on both high-frequency (sub-hourly) and long-term low-frequency (fortnightly-monthly) routine monitoring data. We used resampled high-frequency data to test the uncertainty in water quality parameters (e.g. mean, 95th percentile and load) derived from low-frequency sub-datasets. We showed that the uncertainty in water quality parameters increases with reduced sampling frequency as a function of the c-q slope. We also showed that different sources and delivery pathways control c-q relationship for different solutes and particulates. Secondly, we evaluated the variation in c-q slopes derived from the long-term low-frequency data for different determinands and catchments and showed strong chemostatic behaviour for phosphorus and nitrogen due to saturation and agricultural legacy effects. The c-q slope analysis can provide an effective tool to evaluate the current monitoring networks and the effectiveness of water management interventions. This research highlights how improved understanding of solute and particulate dynamics obtained with optical sensors and analysers can be used to understand patterns in long-term water quality time series, reduce the uncertainty in the monitoring data and to manage eutrophication in agricultural catchments.
Authors
Per-Erik Mellander Philip Jordan Marianne Bechmann Ophélie Fovet Mairead M. Shore Noeleen T. McDonald Chantal Gascuel-OdouxAbstract
Management of agricultural diffuse pollution to water remains a challenge and is influenced by the complex interactions of rainfall-runoff pathways, soil and nutrient management, agricultural landscape heterogeneity and biogeochemical cycling in receiving water bodies. Amplified cycles of weather can also influence nutrient loss to water although they are less considered in policy reviews. Here, we present the development of climate-chemical indicators of diffuse pollution in highly monitored catchments in Western Europe. Specifically, we investigated the influences and relationships between weather processes amplified by the North Atlantic Oscillation during a sharp upward trend (2010– 2016) and the patterns of diffuse nitrate and phosphorus pollution in rivers. On an annual scale, we found correlations between local catchment-scale nutrient concentrations in rivers and the influence of larger, oceanic-scale climate patterns defined by the intensity of the North Atlantic Oscillation. These influences were catchment-specific showing positive, negative or no correlation according to a typology. Upward trends in these decadal oscillations may override positive benefits of local management in some years or indicate greater benefits in other years. Developing integrated climatechemical indicators into catchment monitoring indicators will provide a new and important contribution to water quality management objectives.
Abstract
The estimated potential yield losses caused by plant pathogens is up to 16% globally (Oerke 2006) and most research in plant pathology aims to reduce yield loss in our crops directly or indirectly. Yield losses caused by a certain disease depend not only on disease severity, but also on the weather factors, the pathogen’s aggressiveness, and the ability of the crop to compensate for reduced photosynthetic area. The yield loss-disease relationship in a certain host-pathogen system might therefore change from year to year, making predictions for yield loss very difficult at the regional or even at the farmer’s level. However, estimating yield losses is essential to determine disease management thresholds at which acute control measures such as fungicide applications, or strategic measures such as crop rotation or use of resistant cultivars are economically and environmentally sensible. Legislation in many countries enforces implementation of integrated pest management (IPM), based on economic thresholds at which the costs due to a disease justify the costs for its management. Without a better understanding of the relationship between disease epidemiology and yield loss, we remain insufficiently equipped to design adequate IPM strategies that will be widely adapted in agriculture. Crop loss studies are resource demanding and difficult to interpret for one particular disease, as crops are usually not invaded by only one pest or pathogen at a time. Combining our knowledge on disease epidemiology, crop physiology, yield development, damage mechanisms involved, and the effect of management practices can help us to increase our understanding of the disease-crop loss relationship. The main aim of this paper is to review and analyze the literature on a representative host-pathogen relationship in an important staple food crop to identify knowledge gaps and research areas to better assess yield loss and design management strategies based on economic thresholds. Wheat is one of the most important staple foods worldwide and is susceptible to several important plant diseases. In our article, we focus on Septoria nodorum blotch (SNB) or Glume blotch of wheat as an example for a stubble-borne, seed-transmitted disease with a worldwide distribution causing considerable and regular yield losses. In their review on yield losses due to wheat pathogens in Australia, Murray and Brennan (2009) estimated the current annual economic loss due to SNB as high as $108 × 106, with potential costs as high as $230 × 106. The causal fungus, Parastagonospora nodorum, is currently serving as a model organism for molecular studies of the intimate relationship between necrotic effector-producing fungal strains and their corresponding susceptibility genes present in wheat cultivars (Oliver et al. 2012). In this paper, we analyze the literature on the biology of this common wheat pathogen, the yield loss it reportedly has caused, and the effect of control strategies to reduce this loss. Based on this analysis, we will evaluate the use of common management practices to reduce disease-related yield loss and identify related research needs.
Abstract
This article studies the implementation of the European Union (EU)’s Patients’ Rights Directive in Germany and Norway. The objective of the Directive was to allow EU member states to have a say in the regulatory work, ensure predictability and uniformity in the application of EU rules on cross-border care, and enhance a move towards EU harmonisation in this area. So far, the implementation processes in Norway and Germany have mixed results regarding the likelihood of achieving uniformity and harmonisation. Although the Directive has had convergent effects on certain areas of cross-border care, such as setting up National Contact Points and providing patients with the basic right to treatment abroad, implementation also shows divergent patterns. In both countries, adapting to EU rules has strengthened patients’ rights to choose freely among health-service providers in a wider European healthservice market. However, due to legal discretion and country-specific institutions within which the new rules are applied, divergent patterns prevail.
Abstract
Acetophenones are phenolic metabolites of plant species. A metabolic route for the biosynthesis and release of 2 defence‐related hydroxyacetophenones in white spruce (Picea glauca) was recently proposed to involve 3 phases: (a) biosynthesis of the acetophenone aglycons catalysed by a currently unknown set of enzymes, (b) formation and accumulation of the corresponding glycosides catalysed by a glucosyltransferase, and (c) release of the aglycons catalysed by a glucosylhydrolase (PgβGLU‐1). We tested if this biosynthetic model is conserved across Pinaceae and land plant species. We assayed and surveyed the literature and sequence databases for possible patterns of the presence of the acetophenone aglycons piceol and pungenol and their glucosides, as well as sequences and expression of Pgβglu‐1 orthologues. In the Pinaceae, the 3 phases of the biosynthetic model are present and differences in expression of Pgβglu‐1 gene orthologues explain some of the interspecific variation in hydroxyacetophenones. The phylogenetic signal in the metabolite phenotypes was low across species of 6 plant divisions. Putative orthologues of PgβGLU‐1 do not form a monophyletic group in species producing hydroxyacetophenones. The biosynthetic model for acetophenones appears to be conserved across Pinaceae, whereas convergent evolution has led to the production of acetophenone glucosides across land plants.
Abstract
Question In recent decades, high‐latitude climate has shown regionally variable trends towards warmer and moister conditions. These changes have been predicted to cause afforestation or shrubification of open tundra, increases of warmth‐demanding southern species and plant groups favoured by increased moisture, and decline of species and habitats that are dependent on snow cover. In this study, we explore temporal changes in northern tundra upland plant communities along regional gradients and in local habitats. We ask how vegetation changes are linked with long‐term trends in regional climate and grazing pressure. Location Northern Europe. Methods In 2013–2014, we resurveyed a total of 108 vegetation plots on wind‐exposed and snow‐protected tundra habitats in three subareas along a bioclimatic gradient from the northern boreal to the arctic zone. Vegetation plots were originally sampled in 1964–1967. We related observed vegetation changes to changes in temperature, precipitation and grazing pressure, which all showed regionally variable increases over the study period. Results We found a significant increase of the evergreen dwarf shrub Empetrum nigrum subsp. hermaphroditum in snow‐protected communities and a prominent decrease of lichens throughout the study area. No evidence for extensive tree or larger shrub (Betula spp., Salix spp. or Juniperus communis) encroachment despite climatic warming trends was found. Among studied communities, most pronounced changes in vegetation were observed in snow‐protected boreal heaths on small isolated uplands, where community composition showed low resemblance to the original composition described decades ago. Changes in plant communities correlated with changes in summer and winter temperatures, summer precipitation and reindeer grazing pressure, yet correlations varied depending on region and habitat. Conclusions Northern tundra uplands vary in their resistance to on‐going climate change and reindeer grazing. Isolated treeless heaths of boreal forest–tundra ecotone appear least resistant to climate change and have already shifted towards new community states.
Abstract
The Svalbard Global Seed Vault was opened in 2008. The aim was to secure genetic diversity of crop plants important to future food production. The Seed Vault has the capacity to store 4.5 million seed samples, each containing on average 500 seeds sealed in airtight aluminum bags. By the end of 2016, the Vault had approximately 880,000 accessions representing more than 5000 plant species. The samples, originating from 71 gene banks and research institutes from all across the world, include major food crops such as wheat, rice, barley, sorghum, maize, legumes and forage crops, and vegetables. The seed samples are duplicates (backups) of seed stored in national, regional and international gene banks. Deposits can only be made by following a depositor agreement and the seed samples in the Vault remain the property of the depositing gene bank. The Vault is situated in permafrost at -3 to -4°C, but artificial cooling maintains a temperature of -18°C inside the Vault. Management of the Vault is secured through an agreement between the Norwegian Ministry of Agriculture and Food, the Crop Trust and the Nordic Genetic Resource Centre (NordGen). Secure storage of gene bank seeds in Svalbard was initiated during the 1980s, when the Nordic Gene Bank placed a collection of seed duplicates in an abandoned coal mine outside Longyearbyen in Svalbard. In addition to the secure storage of the base collection, a study of the longevity (germination and seed health) in long-term storage (100 years) in permafrost was started in 1986. A total of 42 seed samples of 16 common agricultural and horticultural Nordic species were included in the study. A set of sub-samples has been taken out for analyses every two and a half years during the first 20 years, and are taken out every five years for the next 80 years.
Abstract
Several studies have shown the positive effect of nitrogen fertilization on conifer growth. In young Norway spruce (Picea abies) stands, an additional effect of including a mixture of other nutrients has often, but not always, been found. We studied effects of repeated fertilization in 28 stands with young Norway spruce in central Norway. The treatments consisted of plots without nutrient addition (Control), fertilization with 150 kg N ha−1 (150 N), and fertilization with 150 kg N plus addition of P, K, Mg, B, Mn and Cu (150 N + mix), repeated three times with approximately eight years interval. There was a clear positive effect on volume increment of the 150 N and 150 N + mix treatments compared to Control, and the effect was significantly higher for 150 N + mix than for 150 N. Fertilization had a stronger effect in the first fertilization period than in the second, while the third period was intermediate. The effect of 150 N + mix was strongest at plots > 300 m a.s.l. However, this correlation may be due to geological conditions rather than elevation. Further studies are needed to find out under which edaphic conditions a nutrient mixture will increase growth substantially in young spruce stands.
Authors
Johannes Deelstra Sekhar Udaya Nagothu Krishna R. Kakumanu Yella R. Kaluvai Surresh R. KallamAbstract
Saving water in irrigated agriculture is a high priority in areas with scarce water resources and impacted by climate change. This paper presents results of measurements on water Productivity (WP) under alternative rice growing practices such as alternating wetting and drying,direct seeded rice, modified systems of rice intensification and conventional paddy rice (NI)in two selected districts (Guntur in Andhra Pradesh and Nalgonda in Telangana, India). Under alternative practices, the yields varied from 5.72 to 6.11 t/ha compared with 4.71 t/ha under paddy rice. The average water application varied from 991 to 1494 mm under alternative practices while average application in conventional paddy rice was 2242 mm. Higher yield and lower water application led to an increase in WP varying from 0.45 to 0.59 kg/m3 under alternative practices compared with 0.22 kg/m3 under conventional paddy rice. The measurements showed that less water can be used to produce more crop under alternative rice growing practices. The results are important for water-scarce areas, providing useful information to policy makers, farmers, agricultural departments and water management boards in devising future climate-smart adaptation and mitigation strategies.
Authors
Kris Verheyen Martin Bažány Ewa Chećko Markéta Chudomelová Déborah Closset-Kopp Patryk Czortek Guillaume Decocq Pieter De Frenne Luc De Keersmaeker Cecilia Enriquez Garcia Martina Fabšičová John-Arvid Grytnes Lucia Hederová Radim Hédl Thilo Heinken Fride Høistad Schei Soma Horváth Bogdan Jaroszewicz Edyta Jermakowicz Terezá Klinerova Jens Kolk Martin Kopecký Iwona Kuras Jonathan Lenoir Martin Macek František Máliš Tone Constance Martinessen Tobias Naaf László Papp Ágnes Papp-Szákaly Paweł Pech Petr Petřík Jindřich Prach Kamila Reczýnska Magne Sætersdal Fabien Spicher Tibor Standovár Krzysztof Świerkosz Ewa Szczęśniak Zoltán Tóth Karol Ujházy Mariana Ujházyová Pieter Vangansbeke Ondřej Vild Dan Wołkowycki Monika Wulf Lander BaetenAbstract
Aim: Revisits of non-permanent, relocatable plots first surveyed several decades ago offer a direct way to observe vegetation change and form a unique and increasingly used source of information for global change research. Despite the important insights that can be obtained from resurveying these quasi-permanent vegetation plots, their use is prone to both observer and relocation errors. Studying the combined effects of both error types is important since they will play out together in practice and it is yet unknown to what extent observed vegetation changes are influenced by these errors. Methods: We designed a study that mimicked all steps in a resurvey study and that allowed determination of the magnitude of observer errors only vs the joint observer and relocation errors. Communities of vascular plants growing in the understorey of temperate forests were selected as study system. Ten regions in Europe were covered to explore generality across contexts and 50 observers were involved, which deliberately differed in their experience in making vegetation records. Results: The mean geographic distance between plots in the observer+relocation error data set was 24 m. The mean relative difference in species richness in the observer error and the observer+relocation data set was 15% and 21%, respectively. The mean “pseudo-turnover” between the five records at a quasi-permanent plot location was on average 0.21 and 0.35 for the observer error and observer+relocation error data sets, respectively. More detailed analyses of the compositional variation showed that the nestedness and turnover components were of equal importance in the observer data set, whereas turnover was much more important than nestedness in the observer+relocation data set. Interestingly, the differences between the observer and the observer+relocation data sets largely disappeared when looking at temporal change: both the changes in species richness and species composition over time were very similar in these data sets. Conclusions: Our results demonstrate that observer and relocation errors are nonnegligible when resurveying quasi-permanent plots. A careful interpretation of the results of resurvey studies is warranted, especially when changes are assessed based on a low number of plots. We conclude by listing measures that should be taken to maximally increase the precision and the strength of the inferences drawn from vegetation resurveys.
Authors
Callum Aidan Stephen Hill Andrew Norton Janka DibdiakovaAbstract
More than sixty environmental product declarations of insulation materials (glass wool, mineral wool, expanded polystyrene, extruded polystyrene, polyurethane, foam glass and cellulose) have been examined and the published information for global warming potential (GWP) and for embodied energy (EE) has been analysed and is presented. A peer-review literature survey of the data for GWP and EE associated with the different insulation products is also included. The data for GWP (kg carbon dioxide equivalents) and EE (megajoules) is reported in terms of product mass or as a functional unit (FU) (1 m2 of insulation with R = 1 m2 K/W). Data for some classes of insulation material (such as glass wool) exhibit a relatively narrow range of values when reported in terms of weight of product or as a functional unit. Other classes of insulation material exhibit much wider distributions of values (e.g., expanded polystyrene). When reported per weight of product, the hydrocarbon-based insulation materials exhibit higher GWP and EE values compared to inorganic or cellulosic equivalents. However, when compared on an FU basis this distinction is no longer apparent and some of the cellulosic based materials (obtained by refining of wood chips) show some of the highest EE values. The relationship between the EE and GWP per kg of insulation product has also been determined as being 15.8 MJ per kg CO2 equivalents.
Authors
Matthias Buchecker Sebastian Eiter Dina Stober Monika Suškevičs Cheryl de Boer Berthe JongejanAbstract
No abstract has been registered
Authors
Dina Stober Matthias Buchecker Berthe Jongejan Sebastian Eiter Monika Suškevičs Stanislav Martinat Igor KuvačAbstract
No abstract has been registered
Authors
Monika Suškevičs Matthias Buchecker Sebastian Eiter Dina Stober Igor Kuvač Berthe Jongejan Stanislav Martinat Cheryl de BoerAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Ingeborg Callesen Nicholas Clarke Andis Lazdinš Iveta Varnagiryte-Kabasinskiene Karsten Raulund-RasmussenAbstract
The long-term carrying capacity for biomass production is highly dependent on available soil resources. A soil test method for potential nutrient release capability was applied to 23 Nordic and Baltic forest soil profiles. The soils had coarse (10), medium (12) and fine (1) soil texture and most were podsolising. Extraction with dilute (0.1 M, 1:50 sample:solution ratio) nitric acid for 2 h was followed by 48 h and 168 h of extraction in soil samples from pedogenetic horizons. Dilute nitric acid solution was replaced after each step and release of mineral nutrient elements in solution was determined. C-horizon nutrient release (µmol g−1 fine earth, 0–218 h) was negatively correlated with mean annual temperature (MAT 0.5–8.5 °C) and for potassium (K) also mean annual precipitation (MAP 523–1440 mm y−1) suggesting a gradient in the mineralogy of the parent material that sediment transports during Pleistocene glaciations have not distorted. In B-horizons of sandy parent materials with felsic mineralogy cumulative nutrient release was positively correlated with pH and with Al and Fe release suggesting accumulation and stabilisation of nutrients in pedogenic products. E-horizons had less nutrient release capability than C-horizons, indicating a more weathered state of E-horizon parent material. Soil formation due to mineral dissolution and leaching of base cations and the gradient in parent material origin and weathering state both affected the observed pattern of nutrient release. On soils with very low mineral P resources (e.g. < 250 kg P ha−1 to 50 cm) by repeated dilute acid extraction, harvest of nutrient rich biomass will not be sustainable. However, it can’t be concluded that sites with high P availability by 0.1 M HNO3 can support an intensive harvest without compensation of P (and Ca) by fertilisation. Due to buffering of removed base cations in B-horizons, nutrient export with biomass may not be traceable as pH decline at decadal time scale. Therefore, the direct measurement of nutrient stocks by the extraction procedure (or other similar assessment of nutrient reserves by strong acid) is suggested as indicative for the mineral weathering capability of forest soils to recover from P and base cation depletion by biomass harvest.
Abstract
Land use change can affect biodiversity, and this has an impact on ecosystem services (ESs), but the relationships between biodiversity and ESs are complex and poorly understood. Biodiversity is declining due to the abandonment of extensively grazed semi-natural grasslands. We therefore aim to explore relationships between biodiversity and ESs provided by extensively managed semi-natural grasslands. Focusing on vascular plant species richness, as well as the ESs fodder quantity, quality, and stability, allergy control, climate regulation, nutrient cycling, pollination, and aesthetic appreciation, we carried out botanical field surveys of 28 paired extensively grazed and abandoned semi-natural grassland plots, with four subplots of 4m2 in each plot. The management of the semi-natural grasslands is and has been at low intensity. We calculated the influence of abandonment on the ES indicators, measured the correlation between the biodiversity measure of vascular plant species richness and ES indicators, and finally determined how the relationships between plant species richness and the ES indicators were affected by the cessation of the extensive management. ES indicators are often, but not always, positively correlated with species richness. Cessation of extensive grazing has both negative and positive effects on ES indicators but the relationships between species richness and ES indicators are often different in extensively managed and abandoned semi-natural grasslands. The relationships between species richness and ES indicators are less pronounced in the extensively managed semi-natural grassland than for the abandoned. One possible reason for this outcome is high functional redundancy in the extensively managed semi-natural grasslands.
Authors
Jens Peter Skovsgaard Jacob Johan Mohr Markmann Giulia Attocchi Bruce TalbotAbstract
The objective of this study was to establish an operational model of productive work time per tree (work efficiency) for high-pruning of young European beech and pedunculate oak depending on tree species, pruning height, branch characteristics, pole saw type and operator. The final model included all of these independent variables with branch characteristics specified in terms of number of live branches and cross-sectional area of the thickest branch at the cut. Work time increased with increasing values of each of the three numeric variables. For a given pruning height the size of the largest branch was for all practical purposes more influential than the number of live branches. Beech took 28% longer to prune than oak. The German Ergo-Schnitt saw was 21% slower than the Japanese Silky Hayauchi saw. The variation in worker performance within our study was larger than that attributed to tree species and pruning equipment.
Academic – Robotic in-row weed control in vegetables
Trygve Utstumo, Frode Urdal, Anders Brevik, ...
Authors
Trygve Utstumo Frode Urdal Anders Brevik Jarle Dørum Jan Netland Øyvind Overskeid Therese With Berge Jan Tommy GravdahlAbstract
Vegetables and other row-crops represent a large share of the agricultural production. There is a large variation in crop species, and a limited availability in specialized herbicides. The robot presented here utilizes systematic growing techniques to navigate and operate in the field. By the use of machine vision it separates seeded vegetable crops from weed. Each weed within the row is treated with individual herbicide droplets, without affecting the crop. This results in a significant reduction in herbicide use, and allows for the use of herbicides that would otherwise harm the crop. The robot is tailored to this purpose with cost, maintainability, efficient operation and robustness in mind. The three-wheeled design is unconventional, and the design maintains maneuverability and stability with the benefit of reduced weight, complexity and cost. Indoor pot trials with four weed species demonstrated that the Drop-on-Demand system (DoD) could control the weeds with as little as 7.6 μg glyphosate or 0.15 μg iodosulfuron per plant. The results also highlight the importance of liquid characteristics for droplet stability and leaf retention properties. The common herbicide glyphosate had no effect unless mixed with suitable additives. A field trial with the robot was performed in a carrot field, and all the weeds were effectively controlled with the DoD system applying 5.3 μg of glyphosate per droplet. The robot and DoD system represent a paradigm shift to the environmental impact and health risks of weed control, while providing a valuable tool to the producers.
Abstract
The key factor for autonomous navigation is efficient perception of the surroundings,while being able to move safely from an initial to a final point. We deal in this paper with a wheeled mobile robot working in a GPS-denied environment typical for a greenhouse. The Hector Simultaneous Localization and Mapping (SLAM) approach is used in order to estimate the robots’ pose using a LIght Detection And Ranging (LIDAR) sensor. Waypoint following and obstacle avoidance are ensured by means of a new artificial potential field (APF) controller presented in this paper. The combination of the Hector SLAMand the APF controller allows themobile robot to performperiodic tasks that require autonomous navigation between predefined waypoints. It also provides themobile robot with a robustness to changing conditions thatmay occur inside the greenhouse, caused by the dynamic of plant development through the season. In this study, we show that the robot is safe to operate autonomously with a human presence, and that in contrast to classical odometrymethods, no calibration is needed for repositioning the robot over repetitive runs. We include here both hardware and software descriptions, as well as simulation and experimental results.
Abstract
In anaerobic digestion, studies of feeding frequency have produced conflicting results. Hence, the effect of feeding frequency on process variables and microbial community structure was investigated by comparing a laboratory-scale digester fed steam exploded food waste 10 times daily vs. one fed an equivalent amount once daily. The Frequently Fed Digester (FFD) produced on average 20% more methane and had lower effluent concentrations of long-chain fatty acids. Greater daily fluctuations in acetate, pH and biogas production rate could explain the lower specific methane yield and β-oxidation. Feeding frequency also influenced the microbial community whereby Tenericutes (42%) dominated in FFD but Firmicutes (31%) was most abundant in the Daily Fed Digester (DFD). Feeding frequency effects are therefore postulated to occur more often in digesters fed labile feedstocks at high organic loading rates.
Authors
Mihaela-Olivia Dobrica Catalin Lazar Lisa Paruch Andre van Eerde Jihong Liu Clarke Catalin Tucureanu Iuliana Caras Sonya Ciulean Adrian Onu Vlad Tofan Alexandru Branzan Stephan Urban Crina Stavaru Norica Branza-NichitaAbstract
Hepatitis B Virus (HBV) infection can be prevented by vaccination. Vaccines containing the small (S)envelope protein are currently used in universal vaccination programs and achieve protective immuneresponse in more than 90% of recipients. However, new vaccination strategies are necessary for successfulimmunization of the remaining non- or low-responders. We have previously characterized a novel HBVchimeric antigen, which combines neutralization epitopes of the S and the preS1 domain of the large (L)envelope protein (genotype D). The S/preS121–47chimera produced in mammalian cells and Nicotianabenthamiana plants, induced a significantly stronger immune response in parenterally vaccinated micethan the S protein. Here we describe the transient expression of the S/preS121–47antigen in an edibleplant, Lactuca sativa, for potential development of an oral HBV vaccine. Our study shows that oral admin-istration of adjuvant-free Lactuca sativa expressing the S/preS121–47antigen, three times, at 1lg/dose,was sufficient to trigger a humoral immune response in mice. Importantly, the elicited antibodies wereable to neutralize HBV infection in an NTCP-expressing infection system (HepG2-NTCP cell line) moreefficiently than those induced by mice fed on Lactuca sativa expressing the S protein. These results sup-port the S/preS121–47antigen as a promising candidate for future development as an edible HBV vaccine.
Authors
Jussi Vuorenmaa Algirdas Augustaitis Burkhard Beudert Witold Bochenek Nicholas Clarke Heleen A de Wit Thomas Dirnböck Jane Frey Hannele Hakola Sirpa Kleemola Johannes Kobler Pavel Krám Antti-Jussi Lindroos Lars Lundin Stefan Löfgren Aldo Marchetto Tomasz Pecka Hubert Schulte-Bisping Krzysztof Skotak Anatoly Srybny Józef Szpikowski Liisa Ukonmaanaho Milan Váňa Staffan Åkerblom Martin ForsiusAbstract
The international Long-Term Ecological Research Network (ILTER) encompasses hundreds of long-term research/monitoring sites located in a wide array of ecosystems that can help us understand environmental change across the globe. We evaluated long-term trends (1990–2015) for bulk deposition, throughfall and runoff water chemistry and fluxes, and climatic variables in 25 forested catchments in Europe belonging to the UNECE International Cooperative Programme on Integrated Monitoring of Air Pollution Effects on Ecosystems (ICP IM). Many of the IM sites form part of the monitoring infrastructures of this larger ILTER network. Trends were evaluated for monthly concentrations of non-marine (anthropogenic fraction, denoted as x) sulphate (xSO4) and base cations x(Ca + Mg), hydrogen ion (H+), inorganic N (NO3 and NH4) and ANC (Acid Neutralising Capacity) and their respective fluxes into and out of the catchments and for monthly precipitation, runoff and air temperature. A significant decrease of xSO4 deposition resulted in decreases in concentrations and fluxes of xSO4 in runoff, being significant at 90% and 60% of the sites, respectively. Bulk deposition of NO3 and NH4 decreased significantly at 60–80% (concentrations) and 40–60% (fluxes) of the sites. Concentrations and fluxes of NO3 in runoff decreased at 73% and 63% of the sites, respectively, and NO3 concentrations decreased significantly at 50% of the sites. Thus, the LTER/ICP IM network confirms the positive effects of the emission reductions in Europe. Air temperature increased significantly at 61% of the sites, while trends for precipitation and runoff were rarely significant. The site-specific variation of xSO4 concentrations in runoff was most strongly explained by deposition. Climatic variables and deposition explained the variation of inorganic N concentrations in runoff at single sites poorly, and as yet there are no clear signs of a consistent deposition-driven or climate-driven increase in inorganic N exports in the catchments.
Abstract
Virus diseases have been a great threat to production of economically important crops. In practice, the use of virus-free planting material is an effective strategy to control viral diseases. Cryotherapy, developed based on cryopreservation, is a novel plant biotechnology tool for virus eradication. Comparing to the traditional meristem culture for virus elimination, cryotherapy resulted in high efficiency of pathogen eradication. In general, cryotherapy includes seven major steps: (1) introduction of infected plant materials into in vitro cultures, (2) shoot tip excision, (3) tolerance induction of explants to dehydration and subsequent freezing in liquid nitrogen (LN), (4) a short-time treatment of explants in LN, (5) warming and post-culture for regeneration, (6) re-establishment of regenerated plants in greenhouse conditions, and (7) virus indexing.
Abstract
This paper compares technical efficiencies (TEs) and technological gap ratios (TGRs) for dairy farms in regions of Norway, accounting for differences in working environments. We used the state-of-the-art stochastic meta-frontier approach to estimate TEs and TGRs to account for regional heterogeneity, and the ‘true’ random-effect model to account for farm effects. The dataset used was farm-level balanced panel data for 24 years (1992–2014), with 5442 observations from 731 dairy farms. The results of the analysis provide empirical evidence of small regional differences in TEs, TGRs, and input use. Furthermore, the results may provide support for the more regionally specific agricultural policy, in terms of support schemes and structural regulations.
Abstract
Viral diseases (a biotic stress) and salinity (an abiotic stress) have been/are the two major constraints for sustainable development of the world’s agricultural production including potato. Crops grown in field are often exposed simultaneously to abiotic and biotic stress, and responses of plants to co-stress by two or more factors may differ from those to each of the multiple stresses. Using in vitro cultures, we demonstrated that virus infection (singly and in combination) or salt, and co-stress by virus infection (singly and in combination) and salt significantly reduced growth and microtuber production, and caused severely oxidative cell damage determined by levels of O2·− and methane dicarboxylic aldehyde, and H2O2 localization in situ. Alterations in physiological metabolism by increasing total soluble sugar and free proline, and by decreasing chlorophyll content are responses of potato plantlets to virus infection (singly and in combination) or salt stress and co-stress by virus infection (singly and in combination) and salt. Oxidative cell damage and reduced chlorophyll content caused by virus and/or salt are believed to be responsible for the reduced growth, eventually resulting in decreased tuber yield. Results reported here would help us to better understand possible mechanism of reduced tuber yield by virus infection and/or salt stress.
Abstract
Blue and yellow sticky traps equipped with blue light emitting diodes (LEDs) were evaluated for their attractiveness to the western flower thrips (Frankliniella occidentalis Pergande) and compared to similar traps without light in two greenhouses with commercial production of either mixed herbs or Alstroemeria cut flowers. Blue traps were more attractive to F. occidentalis than the yellow traps in both crops, regardless of whether they were equipped with light or not. In herbs, the blue light equipped traps caught 1.7 to 2.5 times more thrips compared to blue traps without light, and 1.7 to 3.0 times more thrips than yellow traps with light. Blue light on both blue and yellow traps increased thrips catches in one out of two experiments in Alstroemeria. The blue light equipped traps caught 3.4 and 4.0 times more thrips than blue traps without light in coloured and white Alstroemeria cultivars, respectively, whereas yellow light equipped traps increased thrips catches 4.5 times compared to yellow traps without light in both coloured and white cultivars. The yellow light equipped traps caught, however, only equal to or only slightly more thrips than blue traps without light, and caught fewer thrips than the light equipped blue traps. The relative trapping efficiency of the different combinations of trap colour and light varied with experiment, crop and Alstroemeria cultivars. This suggests that factors other than merely the addition of light influenced the thrips' phototactic response to the traps. Such factors could be differences in the relative strength of the competition between attractive signals from traps and plants between the two crops and Alstroemeria cultivars, thrips density, seasonal lighting conditions or different pest management strategies and other operational procedures in the greenhouses. The light from the traps did not increase the thrips population on the plants below the traps. The implications of the results for thrips control and suggestions for further studies are discussed.
Authors
Svetlana Pakhomova Evgeniy Yakushev Elizaveta Protsenko Sylvain Rigaud Daniel Cossa Joel Knoery Raoul-Marie Couture Olivier Radakovitch Shamil Yakubov Dominika Krzeminska Alice NewtonAbstract
This study presents a specifically designed Mercury module in a coupled benthic-pelagic reactive-transport model - Bottom RedOx Model (BROM) that allows to study mercury (Hg) biogeochemistry under different conditions. This module considers the transformation of elemental mercury (Hg(0)), divalent mercury (Hg(II)) and methylmercury (MeHg). The behavior of mercury species in the model is interconnected with changes of oxygen, hydrogen sulfide, iron oxides, organic matter, and biota. We simulated the transformation and transport of Hg species in the water column and upper sediment layer under five different scenarios, combining various levels of oxygenation and trophic state in the Berre lagoon, a shallow eutrophic lagoon of the French Mediterranean coast subjected to seasonal anoxia. The first scenario represents the conditions in the lagoon that are compared with experimental data. The four other scenarios were produced by varying the biological productivity, using low and high nutrient (N and P) concentrations, and by varying the redox conditions using different intensity of vertical mixing in the water column. The results of the simulation show that both oxidized and reduced sediments can accumulate Hg, but any shifts in redox conditions in bottom water and upper sediment layer lead to the release of Hg species into the water column. Eutrophication and/or restricted vertical mixing lead to reducing conditions and intensify MeHg formation in the sediment with periodic release to the water column. Oxygenation of an anoxic water body can lead to the appearance of Hg species in the water column and uptake by organisms, whereby Hg may enter into the food web. The comparison of studied scenarios shows that a well-oxygenated eutrophic system favors the conditions for Hg species bioaccumulation with a potential adverse effect on the ecosystem. The research is relevant to the UN Minimata convention, EU policies on water, environmental quality standards and Mercury in particular.
Abstract
No abstract has been registered
Authors
Harrison Mburu Laura Cortada Grace Mwangi Kelvin Gitau Agnes Kiriga Zachary Kinyua George Ngundo Wilson Ronno Daniel Leigh Coyne Ricardo Holgado Solveig HaukelandAbstract
No abstract has been registered
Authors
Lise Tingstad John-Arvid Grytnes Vivian Astrup Felde Aino Juslén Esko Hyvärinen Anders DahlbergAbstract
Loss of biodiversity is a pressing global issue, hence it is vital to facilitate informed and effective conservation. As conservation mainly operates at the level of habitats, aiming for species of conservation interest, conservation and management require adequate ecological knowledge of prioritized species for the geographic and environmental setting considered. Our aim was to investigate if ecological documentation in national Red Lists could be combined and used to identify important forest habitats and ecological variables for red-listed forest species in Fennoscandia, and whether this knowledge could be arranged at different geographical scales and for various selections of species of conservation interest. We compiled the national Red Lists of Finland, Norway and Sweden and extracted ecological information for all red-listed forest species (n = 4830). We used a principal component analysis to investigate variation in distribution of species and their habitat associations and taxonomical groups, and to group species of similar associations. We further used the listed species in Sweden as an example, and compared the proportions of species associated to the ecological variables dead wood, living trees or merely the “forest floor and understory” a) at larger and smaller scale (Fennoscandia – county in Sweden), b) in regions with contrasting biomes (nemoral and boreal), and c) in two more limited selections of species of conservation interest; Fennoscandian and globally red-listed species also red-listed in Sweden. Ecological information could be extracted for 96% of the species, albeit with a low resolution; i.e. overall forest habitats, associated tree species, lifeforms and six other ecological variables selected based on their frequent appearance in the Red List documentation. Using this information, we identified five large-scale patterns for Fennoscandian red-listed species; the majority of red-listed species is associated with coniferous forest. The number of red-listed species associated with specific tree species was poorly correlated with the amount of each tree species in Fennoscandia. Dead wood was one of the most important habitat features in terms of number of associated red-listed species, and the proportion of species associated to dead wood was similar in coniferous, boreal and nemoral broadleaved forests types. We demonstrate that ecological documentation in national Red Lists can be used to identify general ecological variables at varying geographical scales and for different selections of species, albeit not with sufficient resolution to provide detailed local conservation guidelines.
Abstract
The prevalence of livestock grazing in wildlife area s is increasing. This transformation of ecosys- tems into agroecosystems is concerning because the intr oduction of new species may cause niche displacement of the functionally related native species. We used a la rge-scale fence scheme and f ecal analyses to study the in fl uence of free-ranging livestock on moose diet on thr ee boreal forest ranges. We found low interspeci fi cdiet overlap between moose and livestock (mean Pianka ’ s O across ranges = 0.21, SD = 0.104), and the diet overlap with livestock did not differ between moose in areas with livestock and in adjacent control areas without live- stock. Still, moose sympatric with livestock had less fe cal nitrogen (a proxy for diet quality) than moose in the control areas. Our fi ndings suggest that interspeci fi c interactions other than direct food competition contributed to reduce the moose ’ foraging opportunities, such as altered forag e abundance and composition, or behavioral avoidance of livestock. We caution that displacement in the foodscape (i.e., spati otemporal use of food) can occur through pathways not evident in niche indices based on composition of plant species in the diet.
Abstract
Background and aims Layered profiles of designed soils may provide long-term benefits for green roofs, provided the vegetation can exploit resources in the different layers. We aimed to quantify Sedum root foraging for water and nutrients in designed soils of different texture and layering. Methods In a controlled pot experiment we quantified the root foraging ability of the species Sedum album (L.) and S. rupestre (L.) in response to substrate structure (fine, coarse, layered or mixed), vertical fertiliser placement (top or bottom half of pot) and watering (5, 10 or 20 mm week−1 ). Results Water availability was the main driver of plant growth, followed by substrate structure, while fertiliser placement only had marginal effects on plant growth. Root foraging ability was low to moderate, as also reflected in the low proportion of biomass allocated to roots (5–13%). Increased watering reduced the proportion of root length and root biomass in deeper layers. Conclusions Both S. album and S. rupestre had a low ability to exploit water and nutrients by precise root foraging in substrates of different texture and layering. Allocation of biomass to roots was low and showed limited flexibility even under water-deficient conditions.
Abstract
PREMISE OF THE STUDY: Genetic differentiation in plant species may result from adaptation to environmental conditions, but also from stochastic processes. The drivers selecting for local adaptation and the contribution of adaptation to genetic differentiation are often unknown. Restoration and succession studies have revealed different colonization patterns for Brachypodium retusum, a common Mediterranean grass. In order to understand these patterns, we tested population differentiation and adaptation to different environmental factors. METHODS: Structured sampling of 12 populations from six sites and two soil types within site was used to analyze the spatial and environmental structure of population differentiation. Sampling sites differ in grazing intensity and climate. We tested germination and growth in a common garden. In subsets, we analyzed the differential response to stone cover, grazing and soil moisture. KEY RESULTS: We found significant differences among populations. The site explained population differentiation better than soil, suggesting a dominant influence of climate and/or genetic drift. Stone cover had a positive influence on seedling establishment, and populations showed a differential response. However, this response was not related to environmental differences between collection sites. Regrowth after clipping was higher in populations from the more intensively grazed Red Mediterranean soils suggesting an adaptation to grazing. Final germination was generally high even under drought, but germination response to differences in soil moisture was similar across populations. CONCLUSIONS: Adaptive population differentiation in germination and early growth may have contributed to different colonization patterns. Thus, the provenance of B. retusum needs to be carefully considered in ecological restoration.
Authors
Michael Roleda Hélène Marfaing Natasa Desnica Rósa Jónsdóttir Jorunn Skjermo Celine Rebours Udo NitschkeAbstract
Seaweeds are increasingly used in European cuisines due to their nutritional value. Many algal constituents, such as polyphenols, are important antioxidants and thus considered beneficial to humans. However, many seaweed species can accumulate heavy metals and exhibit potential health risks upon ingestion. We investigated temporal and spatial variations in polyphenol and heavy metal (As, Cd, Hg, Pb) concentrations of three edible seaweed species. The brown algae Saccharina latissima and Alaria esculenta, and the red alga Palmaria palmata were sourced from natural populations and aquaculture in the NE Atlantic and processed as bulk biomass mimicking industrial scales. The mean polyphenol content was species-specific (Alaria > Saccharina > Palmaria), and highest in winter (for Alaria and Saccharina) and spring (for Palmaria); inter-annual and spatial variations were marginal. Heavy metal concentrations varied between species and depended on collection site, but seasonal variations were minimal. Our data suggest that all three species are good sources of antioxidants, and the heavy metal concentrations are below the upper limits set by the French recommendation and the EU Commission Regulation on contaminants in foodstuffs. A health risk assessment indicated that consumption of these seaweed species poses a low risk for humans with regard to heavy metals. However, an EU-wide regulation on maximal concentration of heavy metals in seaweeds should be established.
Abstract
Sclerotinia stem rot (SSR) is the most important disease of oilseed Brassica crops in Norway. Fungicide applications should be aligned with the actual need for control, but the SSR prediction models used lack accuracy. We have studied the importance of precipitation, and the role of petal and leaf infection for SSR incidence by using data from Norwegian field and trap plant trials over several years. In the trials, SSR incidence ranged from 0 to 65%. Given an infection threshold of 25% SSR, regression and Receiver Operating Characteristics (ROC) analysis were used to evaluate different precipitation thresholds. The sum of precipitation two weeks before and during flowering appeared to be a poor predictor for SSR infection in our field and trap plant trials (P = 0.24, P = 0.11, respectively). Leaves from three levels (leaf one, three, five), and petals were collected at three to four different times during flowering from nine field sites over two years and tested for SSR infection with real-time PCR. Percentage total leaf and petal infection explained 57 and 45% of variation in SSR incidence, respectively. Examining the different leaves and petals separately, infection of leaf three sampled at full flowering showed the highest explanation of variation in later SSR incidence (R2 = 65%, P < 0.001). ROC analysis showed that given an infection threshold of 45%, both petal and leaf infection recommended spraying when spraying was actually needed. Combining information on petal and leaf infection during flowering with relevant microclimate factors in the canopy, instead of the sum of precipitation might improve prediction accuracy for SSR.
Authors
Ari Hietala Isabella Børja Hugh Cross Nina Elisabeth Nagy Halvor Solheim Volkmar Timmermann Adam Vivian-SmithAbstract
European ash (Fraxinus excelsior), a keystone species with wide distribution and habitat range in Europe, is threatened at a continental scale by an invasive alien ascomycete, Hymenoscyphus fraxineus. In its native range of Asia, this fungus is a leaf endophyte with weak parasitic capacity and robust saprobic competence in local ash species that are closely related to European ash. In European ash, H. fraxineus has a similar functional role as in Asia, but the fungus also aggressively kills shoots, resulting in crown dieback and tree death. H. fraxineus is a typical invasive species, as its spread relies on high propagule pressure. While crown dieback of European ash is the most obvious symptom of ash dieback, the annual colonization of ash leaves is a crucial key dependency for the invasiveness of H. fraxineus, since its fruiting bodies are formed on overwintered leaf vein tissues in soil debris. Leaves of European ash host a wide range of indigenous epiphytes, endophytes, facultative parasites and biotrophic fungi, including Hymenoscyphus albidus, a relative of H. fraxineus that competes for the same sporulation niche as the invader. At face value, leaves of European ash are colonized by a large and diverse indigenous mycobiome. In order to understand why this invader became successful in Europe, we discuss and summarize the current knowledge of diversity, seasonal dynamics and traits of H. fraxineus and indigenous fungi associated with leaves of European ash.
Authors
Anita Nussbaumer Peter Waldner Vladislav Apuhtin Fatih Aytar Sue Benham Filippo Bussotti Johannes Eichhorn Nadine Eickenscheidt Petr Fabianek Lutz Falkenried Stefan Leca Martti Lindgren María José Manzano Serrano Stefan Neagu Seppo Nevalainen Jozef Pajtik Nenad Potočić Pasi Rautio Geert Sioen Vidas Stakėnas Celal Tasdemir Iben Margrete Thomsen Volkmar Timmermann Liisa Ukonmaanaho Arne Verstraeten Sören Wulff Arthur GesslerAbstract
Mast seeding, the synchronised occurrence of large amounts of fruits and seeds at irregular intervals, is a reproductive strategy in many wind-pollinated species. Although a series of studies have investigated mast year (MY) patterns in European forest tree species at the regional scale, there are few recent evaluations at a European scale on the impact of weather variables (weather cues) and resource dynamics on mast behaviour. Thus the main objective of this study is to investigate the impact of specific weather conditions, as environmental drivers for MYs, on resources in Fagus sylvatica L., Quercus petraea (Matt.)Liebl., Quercus robur L., Picea abies (L.) Karst. and Pinus sylvestris L. at a European level and to explore the robustness of the relationships in smaller regions within Europe. Data on seed production originating from the International Co-operative Programme on Assessment and Monitoring of Air Pollution Effects on Forests (ICP Forests) were analysed. Three beta regression models were applied to investigate the impact of seasonal weather variables on MY occurrence, as well as the influence of fruiting intensity levels in the years prior to MYs. Resource dynamics are analysed at three different spatial scales (continent, countries and ecoregions). At a European scale, important weather cues for beech MYs were a cold and wet summer two years before a MY, a dry and warm summer one year before a MY and a warm spring in the MY. For spruce, a cold and dry summer two years prior to a MY and a warm and dry summer in the year before the MY showed the strongest associations with the MY. For oak, high spring temperature in the MY was the most important weather cue. For beech and spruce, and to some extent also for oak species, the best fitting models at European scale were well reflected by those found at smaller scales. For pine, best fitting models were highly diverse concerning weather cues. Fruiting levels were high in all species two years before the MY and also high one year before the MY in the oak species and in pine. In beech, fruiting levels one year before the MY were not important and in spruce, they were inconsistent depending on the region. As a consequence, evidence of resource depletion could only be seen in some regions for spruce.
Authors
Anestis Karkanis Georgia Ntatsi Liga Lepse Juan A. Fernández Ingunn M. Vågen Boris Rewald Ina Alsina Arta Kronberga Astrit Balliu Margit Olle Gernot Bodner Laila Dubova Eduardo Rosa Dimitrios SavvasAbstract
Faba beans are highly nutritious because of their high protein content: they are a good source of mineral nutrients, vitamins, and numerous bioactive compounds. Equally important is the contribution of faba bean in maintaining the sustainability of agricultural systems, as it is highly efficient in the symbiotic fixation of atmospheric nitrogen. This article provides an overview of factors influencing faba bean yield and quality, and addresses the main biotic and abiotic constraints. It also reviews the factors relating to the availability of genetic material and the agronomic features of faba bean production that contribute to high yield and the improvement of European cropping systems. Emphasis is to the importance of using new high-yielding cultivars that are characterized by a high protein content, low antinutritional compound content, and resistance to biotic and abiotic stresses. New cultivars should combine several of these characteristics if an increased and more stable production of faba bean in specific agroecological zones is to be achieved. Considering that climate change is also gradually affecting many European regions, it is imperative to breed elite cultivars that feature a higher abiotic–biotic stress resistance and nutritional value than currently used cultivars. Improved agronomical practices for faba bean crops, such as crop establishment and plant density, fertilization and irrigation regime, weed, pest and disease management, harvesting time, and harvesting practices are also addressed, since they play a crucial role in both the production and quality of faba bean.
Authors
Nga Nguyen Marko Suokas Katja Karppinen Jaana Vuosku Laura Jaakola Hely HäggmanAbstract
Bilberry (Vaccinium myrtillus L.) fruits are an excellent natural resource for human diet because of their special favor, taste and nutritional value as well as medical properties. Bilberries are recognized for their high anthocyanin content and many of the genes involved in the anthocyanin biosynthesis have been characterized. So far, neither genomic nor RNA-seq data have been available for the species. In the present study, we de novo sequenced two bilberry fruit developmental stages, unripe green (G) and ripening (R). A total of 57,919 unigenes were assembled of which 80.2% were annotated against six public protein databases. The transcriptome served as exploratory data to identify putative transcription factors related to fruit ripening. Diferentially expressed genes (DEGs) between G and R stages were prominently upregulated in R stage with the functional annotation indicating their main roles in active metabolism and catalysis. The unigenes encoding putative ripening-related regulatory genes, including members of NAC, WRKY, LOB, ERF, ARF and ABI families, were analysed by qRTPCR at fve bilberry developmental stages. Our de novo transcriptome database contributes to the understanding of the regulatory network associated with the fruit ripening in bilberry and provides the frst dataset for wild Vaccinium species acquired by NGS technology.
Academic – Contributors to faecal water contamination in urban environments
Lisa Paruch, Adam Paruch
Abstract
Faecal contamination of water has both anthropogenic and zoogenic origins that can shade various point and nonpoint/diffuse sources of pollution. Due to the dual origin and number of sources of faecal contamination, there are immense challenges in the implementation of effective measures to protect water bodies from pollution that poses threats to human and environmental health. The main health threats refer to infections, illnesses and deaths caused by enteric pathogenicmicrobes, in particular those responsible for waterborne zoonoses. To detect and identify the origins and sources of faecal pollution simultaneously, various methods and indicators have been compiled into a comprehensivemeasuring toolbox. Molecular diagnostics using genetic markers derived from Bacteroidales 16S rRNA gene sequences are quite prevalent in the current methodological implementation for the identification of faecal contamination sources in water. For instance, a culture- and library-independent microbial source tracking toolbox combining micro- and molecular biology tests run as a three-step procedure has been implemented in Norway. Outcomes from the Norwegian studies have identified two general trends in dominance of contributors to faecal water contamination in urban environments. Firstly, there is a tendency of higher contributions from anthropogenic sources during the cold season. Secondly, the identification of the dominance of zoogenic sources in faecalwater contamination during warm periods of the year.
Abstract
Horizontal Visibility Graphs (HVGs) are a recently developed method to construct networks from time series. The values of the time series are considered as the nodes of the network and are linked to each other if there is no larger value between them, such as they can “see” each other. The network properties reflect the nonlinear dynamics of the time series. For some classes of stochastic processes and for periodic time series, analytical results can be obtained for network-derived quantities such as the degree distribution, the local clustering coefficient distribution, the mean path length, and others. HVGs have the potential to discern between deterministic-chaotic and correlated-stochastic time series. Here, we investigate the sensitivity of the HVG methodology to properties and pre-processing of real-world data, i.e., time series length, the presence of ties, and deseasonalization, using a set of around 150 runoff time series from managed rivers at daily resolution from Brazil with an average length of 65 years. We show that an application of HVGs on real-world time series requires a careful consideration of data pre-processing steps and analysis methodology before robust results and interpretations can be obtained. For example, one recent analysis of the degree distribution of runoff records reported pronounced sub-exponential “long-tailed” behavior of North American rivers, whereas another study of South American rivers showed hyper-exponential “short-tailed” behavior resembling correlated noise.We demonstrate, using the dataset of Brazilian rivers, that these apparently contradictory results can be reconciled by minor differences in data-preprocessing (here: small differences in subtracting the seasonal cycle). Hence, data-preprocessing that is conventional in hydrology (“deseasonalization”) changes long-term correlations and the overall runoff dynamics substantially, and we present empirical consequences and extensive simulations to investigate these issues from a HVG methodological perspective. After carefully accounting for these methodological aspects, the HVG analysis reveals that the river runoff dataset shows indeed complex behavior that appears to stem from a superposition of short-term correlated noise and “long-tailed behaviour,” i.e., highly connected nodes. Moreover, the construction of a dam along a river tends to increase short-term correlations in runoff series. In summary, the present study illustrates the (often substantial) effects of methodological and data-preprocessing choices for the interpretation of river runoff dynamics in the HVG framework and its general applicability for real-world time series.
Authors
Matthew Schwarzkopf Michael Burnard Viacheslav Tverezovskiy Andreas Treu Miha Humar Andreja KutnarAbstract
Within the Slovenian region of Istria, the olive growing and oil production industry is strong. This industry has a long history and the olives grown here have high levels of biologically active compounds including a variety of phenolic compounds. Using residual materials generated by this industry in potential wood protection systems would not only valorise low-value materials and stimulate rural economies but would also provide an alternative to currently used oil-based protection systems. The objective of this study was to produce an oil treatment for wood protection and assess its efficacy in reducing leaching, weathering effects, and fungal decay. Two maleinisation techniques were used to chemically modify low-value lampante oil in an attempt to limit leaching when impregnated in wood. Pinus sylvestris (Scots pine) and Fagus sylvatica (European beech) were treated with the modified oils and underwent leaching, accelerated weathering, and decay tests. Leaching of the treatment oils was relatively low compared with other experiments and beech wood specimens treated with a direct maleinisation treatment showed improvement in performance compared to control specimens. In addition, it was found that the modified oils were not completely removed from the wood after solvent extraction indicating that they could potentially be used as an immobilisation agent in combination with other treatments thereby reducing the amount of active component of the protective agent.
Abstract
Root rot in Norway spruce (Picea abies (L.) Karst.) causes substantial economic losses to the forestry sector. In this study, we developed a probability model for decay at breast height utilizing 18,141 increment cores sampled on temporary plots of the Norwegian National Forest Inventory. The final model showed a good fit to the data and retained significant relationships between decay and a suite of tree, stand and site variables, including diameter at breast height, stand age, altitude, growing season temperature sum (threshold 5°C), and vegetation type. By comparing model predictions with recorded decay at stump height in an independent data set, we estimated a proportionality function to adjust for the inherent underestimation of total rot that will be obtained by applying a probability model derived from increment cores sampled at breast height. We conclude that the developed model is appropriate for national and regional scenario analyses in Norway, and could also be useful as a tool for operational forestry planning. This would however require further testing on independent data, to assess how well the new model predicts decay at local scales.
Authors
Shahid Nadeem Håvard Steinshamn Elin Halvorsen Sikkeland Anne-Maj Gustavsson Anne Kjersti BakkenAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Yanliang Wang Erik Lysøe Tegan Armarego-Marriott Alexander Erban Lisa Paruch Andre van Eerde Ralph Bock Jihong Liu ClarkeAbstract
No abstract has been registered
Abstract
Dieback of European ash, caused by the ascomycete Hymenoscyphus fraxineus originating from Asia, has rapidly spread across Europe, and is threatening this keystone tree at a continental scale. High propagule pressure is characteristic to invasive species. Consistently, the enormous production of windborne ascospores by H. fraxineus in an ash forest with epidemic level of disease obviously facilitates its invasiveness and long distance spread. To understand the rate of build-up of propagule pressure by this pathogen following its local introduction, during 2011–2017 we monitored its sporulation at a newly infested ash stand in south-western Norway characterized with mild winters and cool summers. We also monitored the propagule pressure by Hymenoscyphus albidus, a non-pathogenic native species that competes for the same sporulation niche with H. fraxineus. During the monitoring period, crown condition of ash trees had impaired, and 20% of the dominant trees were severely damaged in 2017. H. fraxineus showed an exponential increase in spore production between 2012 and 2015, followed by drastic decline in 2016 and 2017. During 2011–2013, the two Hymenoscyphus species showed similar sporulation level, but thereafter spores of H. albidus were no longer detected. The data suggest that following local introduction, the population of H. fraxineus reaches rapidly an exponential growth stage if the local weather conditions are favorable for ascomata maturation across years. In the North Atlantic climate, summer temperatures critically influence the pathogen infection pressure, warm summers allowing the population to grow according to its biotic potential, whereas cold summers can cause a drastic decline in propagule pressure.
Authors
Jonathan Rizzi Irene Brox Nilsen James Howard Stagge Kjersti Gleditsch Gisnås Lena M. TallaksenAbstract
Northern latitudes are experiencing faster warming than other regions in the world, which is partly explained by the snow albedo feedback. In Norway, mean temperatures have been increasing since the 1990s, with 2014 being the warmest year on record, 2.2 °C above normal (1961–1990). At the same time, a concurrent reduction in the land area covered by snow has been reported. In this study, we present a detailed spatial and temporal (monthly and seasonal) analysis of trends and changes in snow indices based on a high resolution (1 km) gridded hydro-meteorological dataset for Norway (seNorge). During the period 1961–2010, snow cover extent (SCE) was found to decrease, notably at the end of the snow season, with a corresponding decrease in snow water equivalent except at high elevations. SCE for all Norway decreased by more than 20,000 km2 (6% of the land area) between the periods 1961–1990 and 1981–2010, mainly north of 63° N. Overall, air temperature increased in all seasons, with the highest increase in spring (particularly in April) and winter. Mean monthly air temperatures were significantly correlated with the monthly SCE, suggesting a positive land–atmosphere feedback enhancing warming in winter and spring.
Abstract
Over their life course, people change their consumption habits when prices, income, tastes or nutritional needs change. The time period during which an individual grew up is often reflected in his or her consumption of different types of food. To investigate the possible links between demographic changes and food consumption, we constructed two-step censored demand systems for different groups of foods. We estimated the systems using Norwegian data for the 1986 – 2012 period. In the systems, age, period, cohort, other demographic and economic variables are included. The estimated systems are used to construct a long-run forecasting model for meat and dairy products. In this model, younger cohorts replace older cohorts with a different consumption pattern. The total purchases of beef, lamb, pork and fluid milk are predicted to decrease, while the total purchases of chicken, yoghurt and cheese are predicted to increase towards 2027.
Abstract
Purpose Treelines and forest lines (TFLs) have received growing interest in recent decades, due to their potential role as indicators of climate change. However, the understanding of TFL dynamics is challenged by the complex interactions of factors that control TFLs. The review aims to provide an overview over the trends in the elevational dynamics of TFLs in Norway since the beginning of the 20th century, to identify main challenges to explain temporal and spatial patterns in TFL dynamics, and to identify important domains for future research. Method A systematic search was performed using international and Norwegian search engines for peer-reviewed articles, scientific reports, and MA and PhD theses concerning TFL changes. Results Most articles indicate TFL rise, but with high variability. Single factors that have an impact on TFL dynamics are well understood, but knowledge gaps exist with regard to interactions and feedbacks, especially those leading to distributional time lags. Extracting the most relevant factors for TFL changes, especially with regard to climate versus land-use changes, requires more research. Conclusions Existing data on TFL dynamics provide a broad overview of past and current changes, but estimations of reliable TFL changes for Norway as a whole is impossible. The main challenges in future empirically-based predictions of TFLs are to understand causes of time lags, separate effects of contemporary processes, and make progress on the impacts of feedback and interactions. Remapping needs to be continued, but combined with both the establishment of representative TFL monitoring sites and field experiments.
Authors
Alan C. Gange Einar Heegaard Lynne Boddy Carrie Joy Andrew Paul M. Kirk Rune Halvorsen Thomas W. Kuyper Claus Bässler Jefferey M. Diez Jacob Heilman-Clausen Klaus Høiland Ulf Büntgen Håvard KauserudAbstract
Despite the dramatic phenological responses of fungal fruiting to recent climate warming, it is unknown whether spatial distributions of fungi have changed and to what extent such changes are influenced by fungal traits, such as ectomycorrhizal (ECM) or saprotrophic lifestyles, spore characteristics, or fruit body size. Our overall aim was to understand how climate and fungal traits determine whether and how species‐specific fungal fruit body abundances have shifted across latitudes over time, using the UK national database of fruiting records. The data employed were recorded over 45 yr (1970–2014), and include 853 278 records of Agaricales, Boletales and Russulales, though we focus only on the most common species (with more than 3000 records each). The georeferenced observations were analysed by a Bayesian inference as a Gaussian additive model with a specification following a joint species distribution model. We used an offset, random contributions and fixed effects to isolate different potential biases from the trait‐specific interactions with latitude/climate and time. Our main aim was assessed by examination of the three‐way‐interaction of trait, predictor (latitude or climate) and time. The results show a strong trait‐specific shift in latitudinal abundance through time, as ECM species have become more abundant relative to saprotrophic species in the north. Along precipitation gradients, phenology was important, in that species with shorter fruiting seasons have declined markedly in abundance in oceanic regions, whereas species with longer seasons have become relatively more common overall. These changes in fruit body distributions are correlated with temperature and rainfall, which act directly on both saprotrophic and ECM fungi, and also indirectly on ECM fungi, through altered photosynthate allocation from their hosts. If these distributional changes reflect fungal activity, there will be important consequences for the responses of forest ecosystems to changing climate, through effects on primary production and nutrient cycling.
Authors
Bianca CavicchiAbstract
This paper analyses the case of bioenergy development in Norway – drawing on Hedmark county located on the borders with Sweden – from a social, economic and environmental perspective (triple bottom line). Since 2008, the number of forest-based bioenergy plants increased rapidly, following the introduction of the wood-chips scheme and the high local expectations of its benefits for rural development. Obstacles to its continuous sustainable development have subsequently been increasing. Therefore, the goal of the study is to investigate the causal processes of bioenergy development to understand what threatens its triple bottom line sustainability. The study does so by employing qualitative system dynamics (i.e. causal loop diagram) and using interviews with local actors to elaborate on studies that look at the influence of power, institutions and expectations on the transition processes. Results show that the local actors’ positive perceptions of the benefits of bioenergy mainly drove its initial development, but that conflicting local interests, power relations, and market dynamics now threaten these initially positive perceptions.
Authors
Daniel Girma Mulat Janka Dibdiakova Svein Jarle HornAbstract
Background: The emerging cellulosic bioethanol industry will generate huge amounts of lignin-rich residues that may be converted into biogas by anaerobic digestion (AD) to increase the output of energy carriers from the biorefnery plants. The carbohydrates fraction of lignocellulosic biomass is degradable, whereas the lignin fraction is generally considered difcult to degrade during AD. The objective of this study was to investigate the feasibility of biogas production by AD from hydrolysis lignin (HL), prepared by steam explosion (SE) and enzymatic saccharifcation of birch. A novel nylon bag technique together with two-dimensional nuclear magnetic resonance spectroscopy, pyrolysis–gas chromatography–mass spectrometry (Py-GC/MS), and Fourier transform infrared (FTIR) spectroscopy was used to identify recalcitrant and degradable structures in the lignin during AD. Results: The HL had a lignin content of 80% which included pseudo-lignin and condensed-lignin structures resulting from the SE pretreatment. The obtained methane yield from HL was almost twofold higher than the theoretical methane from the carbohydrate fraction alone, indicating that part of the lignin was converted to methane. Characterization of the undegradable material after AD revealed a substantial loss of signals characteristic for carbohydrates and lignin–carbohydrate complexes (LCC), indicating conversion of these chemical components to methane during AD. The β-O-4′ linkage and resinol were not modifed as such in AD, but major change was seen for the S/G ratio from 5.8 to 2.6, phenylcoumaran from 4.9 to 1.0%, and pseudo-lignin and condensed-lignin were clearly degraded. Scanning electron microscopy and simultaneous thermal analysis measurements demonstrated changes in morphology and thermal properties following SE pretreatment and AD. Our results showed that carbohydrate, LCC, pseudo-lignin, and condensed-lignin degradation had contributed to methane production. The energy yield for the combined ethanol production and biogas production was 8.1 MJ fuel per kg DM of substrate (4.9 MJ/kg from ethanol and 3.2 MJ/kg from methane). Conclusion: This study shows the beneft of using a novel bag technique together with advanced analytical techniques to investigate the degradation mechanisms of lignin during AD, and also points to a possible application of HL produced in cellulosic bioethanol plants.
Abstract
Runoff prediction in ungauged catchments has been a challenging topic over recent decades. Much research have been conducted including the intensive studies of the PUB (Prediction in Ungauged Basins) Decade of the International Association for Hydrological Science. Great progress has been made in the field of regionalization study of hydrological models; however, there is no clear conclusion yet about the applicability of various methods in different regions and for different models. This study made a comprehensive assessment of the strengths and limitations of existing regionalization methods in predicting ungauged stream flows in the high latitudes, large climate and geographically diverse, seasonally snow-covered mountainous catchments of Norway. The regionalization methods were evaluated using the water balance model – WASMOD (Water And Snow balance MODeling system) on 118 independent catchments in Norway, and the results show that: (1) distance-based similarity approaches (spatial proximity, physical similarity) performed better than regression-based approaches; (2) one of the combination approaches (combining spatial proximity and physical similarity methods) could slightly improve the simulation; and (3) classifying the catchments into homogeneous groups did not improve the simulations in ungauged catchments in our study region. This study contributes to the theoretical understanding and development of regionalization methods.
Abstract
Improved waterlogging tolerance of wheat and barley varieties may alleviate yield constraints caused by heavy or long-lasting precipitation. The waterlogging tolerance of 181 wheat and 210 barley genotypes was investigated in field trials between 2013 and 2014. A subset of wheat genotypes were selected for yield trials in 2015 and 2016. Our aim was to: (1) characterize the waterlogging tolerance of genotypes with importance for Norwegian wheat and barley breeding, and (2) identify which phenotypic traits that most accurately determine the waterlogging tolerance of wheat in our field trials. Waterlogging tolerance was determined by principal component analysis (PCA) where best linear unbiased predictors (BLUPs) of the traits chlorosis, relative plant height, heading delay, relative spike number, relative biomass and an overall condition score were used as input variables. Six wheat and five barley genotypes were identified as consistently more tolerant in 2013 and 2014. This included the waterlogging tolerant CIMMYT line CETA/Ae. tauschii (895). Chlorosis and the overall condition score were the traits that best explained the yield response of the genotypes selected for the yield trials. Our results show that early stress symptoms did not necessarily reflect the ability to recover post treatment. Thus, records from full crop cycles appear as fundamental when screening populations with unknown tolerance properties.
Abstract
It is recognised that flame retardant chemicals degrade and leach out of flame-protected wood claddings when exposed to natural weathering. However, the ability to survey the current state of a flame retardant treatment applied to a wood cladding, an arbitrary length of time after the initial application, is limited today. In this study, hyperspectral imaging in the near infrared to short-wavelength infrared region is used to quantify the amount of flame retardant present on wooden surfaces. Several sets of samples were treated with various concentrations of a flame retardant chemical and scanned with a push broom hyperspectral camera. An inductively coupled plasma (ICP) spectroscopy analysis of the outermost layer of the treated samples was then carried out in order to determine each sample’s phosphorus content, the active ingredient in the flame retardant. Spectra from the hyperspectral images were pre-processed with extended multiplicative scatter correction, and the phosphorus content was modelled using a partial least squares (PLS) regression model. The PLS regression yielded robust predictions of surface phosphorus content with a coefficient of determination, R2, between 0.8 and 0.9 on validation data regardless of whether the flame retardant chemical had been applied to the surface of the wood or pressure-impregnated into it. The result from the study indicates that spectral imaging around the 2400–2531nm wavelength region is favourable for quantifying the amount of phosphorus-based flame retardant contained in the outermost layer of non-coated wooden claddings. The results also reveal that the uptake of phosphorus-based flame retardant does not occur uniformly throughout the wood surface, but is to a larger extent concentrated in the earlywood regions than in the latewood.
Authors
Trust Kasambala Donga Ole Martin EkloAbstract
The sugarcane industry is the third largest user of pesticides in Malawi. Our aim with this study was to document pesticide use and handling practices that influence pesticide exposure among sugarcane farmers in Malawi. A semi-structured questionnaire was administered to 55 purposively selected sugarcane farmers and 7 key informants representing 1474 farmers in Nkhata Bay, Nkhotakota and Chikwawa Districts in Malawi. Our results indicate that herbicides and insecticides were widely used. Fifteen moderately and one extremely hazardous pesticide, based on World Health Organization (WHO) classification, were in use. Several of these pesticides: ametryn, acetochlor, monosodium methylarsonate and profenofos are not approved in the European Union because of their toxicity to terrestrial and aquatic life, and/or persistence in water and soil. Farmers (95%) knew that pesticides could enter the human body through the skin, nose (53%) and mouth (42%). They knew that pesticide runoff (80%) and leaching (100%) lead to contamination of water wells. However, this knowledge was not enough to motivate them to take precautionary measures to reduce pesticide exposure. Farmers (78%) had experienced skin irritation, 67% had headache, coughing and running nose during pesticide handling. Measures are in place to reduce pesticide exposure in the large estates and farms operated by farmer associations. Smallholder farmers acting independently do not have the resources and capacity to minimize their exposure to pesticides. There is need to put in place pesticide residue monitoring programs and farmer education on commercial sugarcane production and safe pesticide use as ways of reducing pesticide exposure.
Abstract
The research literature on food selection by large herbivores is extensive. Still, we are generally lacking in our knowledge of the influence of potentially interacting chemical contents of the food. We made a qualitative review of a systematic literature search of studies that empirically link chemical contents of food to the food selection by northern cervids (genera Alces, Capreolus, Cervus, Dama, Odocoileus, Rangifer). We found that although the majority of the 98 relevant studies measuring any given food constituent (energy, protein, fiber, minerals, plant secondary metabolites) provided support for it acting as a driver of food selection (in either a negative or positive way), there was little support for the traditional hypotheses of maximization or limitation of any single constituent. Rather, because of the animals’ need to acquire an appropriate intake of several constituents at the same time, our review highlights how new empirical stud- ies need to focus on several food constituents in synchrony: (1) Study designs should capture sufficient variation in the content of food constituents in order to tease apart their many co-variations; and (2) insights about nutritional drivers may be lost if one uses only composite currencies such as crude energy, crude fiber, ash, or tannins, which may mask contrasting selection patterns of the lumped constituents. Season had an apparent influence on the selection of some food constituents, particularly various fiber frac- tions. In contrast, our review revealed a lack of evidence that cervids more strongly select for protein in summer than they do in winter. Our overall conclusion of the review is that interacting chemical contents of food make the nutritional value of a given food type into a varying entity. To better elucidate this varia- tion, we need new technologies that non-invasively capture nutrient intake of free-ranging animals, across seasons.
Abstract
Small mammals, especially microtine rodents, play an important role in the dynamics of boreal forest ecosystems. Even-aged forest management, in which old, semi-natural forests are converted to clear-cuts and culturally regenerated stands, is expected to have pronounced impact on the abundance and composition of this group of animals due to changes in the understory vegetation. During a 39 year-period we sampled autumn numbers of small mammals in uncut, semi-natural old forest and in recent clearcuts, supplemented by a 7-year sample from middle-aged plantations. Field voles Microtus agrestis were almost exclusively trapped in clearcuts. Bank voles Myodes glareolus dominated in the old forest, but reached equal or higher densities than field voles in clearcuts. Here, their combined abundance exceeded that of bank voles in old forest. Some years, wood lemmings Myopus schisticolor contributed significantly to vole abundance in old forest. Other rodents Apodemus spp. were rarely captured, mainly in clearcuts, and shrews Sorex spp. numbered < 15 percent of the total number of captured animals. Throughout the whole period we discerned 11 vole cycles, with highest peaks in bank voles in old forest. After high numbers during the 1980s, abundances of all species fell markedly during the 1990s, most distinctively in clearcuts, where the field vole almost totally disappeared. From the late 2000s, abundances of all species returned to pre-1990 levels and beyond. In the early and late periods, combined vole numbers were 26% higher in clearcuts compared to old forest, whereas the opposite was true in the middle period. In middle-aged plantations, bank voles numbered only one third of what it was in clearcuts and old forest, and other voles were rarely trapped. The results support the general notion that bank voles thrive in bilberry-rich, older forest and field voles in grass-dominated habitat. Contrary to general assertions, bank vole was abundant also in clearcuts, possibly due to invasion from surrounding old forest, but peak densities were lower than in old forest, possibly due to suppression by field voles. The variation of small mammals in forest age classes concurred closely with recent results reported from Finland. On a landscape scale, the results from these two and other studies predict that the total biomass of small rodents will be reduced by even-aged forest management, not because of conversion of older, semi-natural forest to clearcuts, but because of a decline in numbers in middle-aged and older, secondary forests.
Abstract
The aim of the investigation was to assess and compare the environmental limits for growth cessation and floralinitiation in a range of new and established biennial-fruiting red raspberry (Rubus idaeus L.) cultivars of diverseorigin under phytotron and field conditions. The results confirmed that growth cessation and floral initiation inbiennial-fruiting red raspberry are jointly controlled by the interaction of low temperature and short days (SD).When transferred from non-inductive high temperature and long day (LD) conditions to naturally decreasingautumn daylengths at varying phytotron temperatures on 18 August, growth immediately levelled off and ceasedcompletely within 2 weeks in all cultivars at 9 °C. Serial dissections of lateral buds revealed that floral initiationsimultaneously took place. At 15 °C on the other hand, the plants continued growing and remained vegetativeuntil around 15 September when the daylength had decreased to approximately 13 h. The change to 9 °C resultedin an immediate but short-lasting floral induction response that did not bring about initiation in buds situatednear the base of the canes, as was the case at 15 °C. At 18 °C, marginal floral induction took place only in thecultivars ‘Glen Ample’, ‘Balder’ and ‘Vene’, even at photoperiods down to 10 h, whereas at 21 °C, all cultivarsgrew vegetatively regardless of daylength conditions. However, exceptions were some plants of ‘Vene’ and‘Anitra’ that initiated terminal flowers at 18 and 21 °C and flowered directly without chilling (so-called tipflowering). Although some cultivars of Northern origin ceased growing and initiated floral primordia somewhatearlier (at longer photoperiods) than those of more southerly origin, the differences were relatively minor andnot consistent (no latitudinal cline). Results obtained in the field under decreasing autumn temperature anddaylength conditions agreed closely with the results in the phytotron. We therefore conclude that results ob-tained with raspberry in properly controlled daylight phytotron experiments are generally applicable to fieldconditions.
Authors
Trine Aulstad Sogn Ivan Dragicevic Roar Linjordet Tore Krogstad Vincent Eijsink Susanne Eich-GreatorexAbstract
Purpose The main purposes of the study were to assess the NPK fertilizer value of biogas digestates in different soils and to evaluate the risk of unwanted nutrient leaching. Methods The fertilizer value of digestates from anaerobic digesters was investigated in a greenhouse pot experiment with wheat in three different soils; silt, loam and sand. The digestates were based on different feedstock and had a low, dry matter content. The fertilizing effect of digestates was compared to mineral fertilizer and manure. To investigate the fate of excess nutrients in soil after the growing season, the pots were leached after harvest. A complementary soil column leaching experiment without plants was carried out in the laboratory. Results The concentration of ammonium in digestates provided a good indicator of the nitrogen fertilizer value of the digestates. In the silt and loam, the ammonium N fraction in digestates had a fertilizer replacement value equal to that of mineral fertilizer N, whereas the replacement value was higher in the nutrient poor sandy soil. Digestates often have a ratio between nitrogen, phosphorus and potassium which is not favourable for plant growth. However, the suboptimal balance did not result in reduced plant growth or unwanted leaching from soil. Conclusions The results show that digestates from biogas production based on fundamentally different feedstock are promising as NPK fertilizers. The N fertilization can simply be based on the digestate NH4+ concentration and, at least for wheat production, considerable variation in the concentrations of K and P can be tolerated.
Abstract
We investigated whether diversification and/or structural change would improve Norwegian agriculture. Using a flexible technology approach to account for different technologies, we assessed economies of scope and scale of dairy and cropping farms, including regional differences. We fitted translog cost functions to farm-level panel data for the period 1991–2014. We found both economies of scope and scale on the farms. Dairy farms have an economic incentive to integrate dairying with cropping in all regions of Norway, and vice versa. Thus, policy makers should eschew interventions that inhibit diversification or structural change and that increase the costs of food production.
Authors
Arild AndersenAbstract
The present paper is the last in a series of four on the fauna of Agromyzidae in Norway, and deals with the genera Melanagromyza Hendel, 1920, Ophiomyia Braschnikov, 1897, Amauromyza Hendel, 1931, Aulagromyza Enderlein, 1936, Cerodontha Rondani, 1861, Chromatomyia Hardy, 1849, Liriomyza Mik, 1894, Metopomyza Enderlein, 1936, Napomyza Westwood, 1840 and Phytomyza Fallén, 1810. Ninety-six species are reported of which seventeen are reported new to the Norwegian fauna: Melanagromyza aeneoventris (Fallén, 1823), M. cunctans (Meigen, 1830), M. pubescens Hendel, 1923, M. submetallescens Spencer, 1966, Ophiomyia curvipalpis (Zetterstedt, 1848), O. ranunculicaulis Hering, 1949, Chromatomyia syngenesiae Hardy, 1849, Metopomyza interfrontalis Melander, 1913, M. xanthaspioides (Frey, 1946) , Phytomyza cecidonomia Hering, 1937, P. cirsii Hendel, 1923, P. clematidis Kaltenbach, 1859, P. fennoscandiae Spencer, 1976, P. isais Hering, 1937, P. origani Hering, 1931, P. pulsatillae Hering, 1924 and P. socia Brischke, 1881. In addition, new regional data is given for eighty species previously reported from Norway. The biology of the larva, when known, and the distribution in Norway and Europe are commented on species new to Norway. The Norwegian checklist for Agromyzidae now consist of 256 species.
Abstract
Given the compound differences between tris(2-butoxyethyl)- and tris(2-cloroethyl) phosphate (TBOEP and TCEP, respectively), we hypothesized that exposure of juvenile salmon to TBOEP and TCEP will produce compound-specific differences in uptake and bioaccumulation patterns, resulting in potential formation of OHmetabolites. Juvenile salmon were exposed to waterborne TCEP or TBOEP (0.04, 0.2 and 1 mg/L) for 7 days. The muscle accumulation was measured and bioconcentration factor (BCF) was calculated, showing that TCEP was less accumulative and resistant to metabolism in salmon than TBOEP. Metabolite formations were only detected in TBOEP-exposed fish, showing seven phase I biotransformation metabolites with hydroxylation, ether cleavage or combination of both reactions as important metabolic pathways. In vitro incubation of trout S9 liver fraction with TBOEP was performed showing that the generated metabolite patterns were similar to those found in muscle tissue exposed in vivo. However, another OH-TBOEP isomer and an unidentified metabolite not present in in vivo exposure were observed with the trout S9 incubation. Overall, some of the observed metabolic products were similar to those in a previous in vitro report using human liver microsomes and some metabolites were identified for the first time in the present study. Toxicological analysis indicated that TBOEP produced less effect, although it was taken up faster and accumulated more in fish muscle than TCEP. TCEP produced more severe toxicological responses in multiple fish organs. However, liver biotransformation responses did not parallel the metabolite formation observed in TBOEP-exposed fish.
Authors
Sebastian Sippel Markus Reichstein Xuanlong Ma Miguel D. Mahecha Holger Lange Milan Flach Dorothea FrankAbstract
Purpose of the Review Weather and climate extremes substantially affect global- and regional-scale carbon (C) cycling, and thus spatially or temporally extended climatic extreme events jeopardize terrestrial ecosystem carbon sequestration. We illustrate the relevance of drought and/or heat events (“DHE”) for the carbon cycle and highlight underlying concepts and complex impact mechanisms. We review recent results, discuss current research needs and emerging research topics. Recent Findings Our review covers topics critical to understanding, attributing and predicting the effects of DHE on the terrestrial carbon cycle: (1) ecophysiological impact mechanisms and mediating factors, (2) the role of timing, duration and dynamical effects through which DHE impacts on regional-scale carbon cycling are either attenuated or enhanced, and (3) large-scale atmospheric conditions under which DHE are likely to unfold and to affect the terrestrial carbon cycle. Recent research thus shows the need to view these events in a broader spatial and temporal perspective that extends assessments beyond local and concurrent C cycle impacts of DHE. Summary Novel data streams, model (ensemble) simulations, and analyses allow to better understand carbon cycle impacts not only in response to their proximate drivers (drought, heat, etc.) but also attributing them to underlying changes in drivers and large-scale atmospheric conditions. These attribution-type analyses increasingly address and disentangle various sequences or dynamical interactions of events and their impacts, including compensating or amplifying effects on terrestrial carbon cycling.
Authors
Thiago Castro Rafael de Andrade Moral Clarice Garcia Borges Demétrio Italo Jr. Delalibera Ingeborg KlingenAbstract
Neozygites floridana is a pathogenic fungus and natural enemy of the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae), which is an important polyphagous plant pest. The aim of this study was to reveal and predict what combination of temperature, relative humidity (RH), and time that enables and promotes primary conidia production and capilliconidia formation in N. floridana (Brazilian isolate ESALQ 1420), in both a detached leaf assay mimicking climatic conditions in the leaf boundary layer and in a semi-field experiment. In the detached leaf assay, a significant number of conidia were produced at 90% RH but the highest total number of primary conidia and proportion of capilliconidia was found at 95 and 100% RH at 25 °C. Positive temperature and RH effects were observed and conidia production was highest in the 8 to 12 h interval. The semi-field experiment showed that for a >90% probability of N. floridana sporulation, a minimum of 6 h with RH >90% and 10 h with temperatures >21 °C, or 6 h with temperatures >21 °C and 15 h with RH >90% was needed. Our study identified suitable conditions for primary- and capilliconidia production in this Brazilian N. floridana isolate. This information provides an important base for building models of a Decision Support System (DSS) where this natural enemy may be used as a tool in Integrated Pest Management (IPM) and a base for developing in vivo production systems of N. floridana.
Authors
Ryan Bright Stephanie Eisner Marianne Tronstad Lund Titta Majasalmi Gunnar Myhre Rasmus AstrupAbstract
Predicting the surface albedo of a forest of a given species composition or plant functional type is complicated by the wide range of structural attributes it may display. Accurate characterizations of forest structure are therefore essential to reducing the uncertainty of albedo predictions in forests, particularly in the presence of snow. At present, forest albedo parameterizations remain a nonnegligible source of uncertainty in climate models, and the magnitude attributable to insufficient characterization of forest structure remains unclear. Here we employ a forest classification scheme based on the assimilation of Fennoscandic (i.e., Norway, Sweden, and Finland) national forest inventory data to quantify the magnitude of the albedo prediction error attributable to poor characterizations of forest structure. For a spatial domain spanning ~611,000 km2 of boreal forest, we find a mean absolute wintertime (December–March) albedo prediction error of 0.02, corresponding to a mean absolute radiative forcing ~0.4 W/m2. Further, we evaluate the implication of excluding albedo trajectories linked to structural transitions in forests during transient simulations of anthropogenic land use/land cover change. We find that, for an intensively managed forestry region in southeastern Norway, neglecting structural transitions over the next quarter century results in a foregone (undetected) radiatively equivalent impact of ~178 Mt‐CO2‐eq. year−1 on average during this period—a magnitude that is roughly comparable to the annual greenhouse gas emissions of a country such as The Netherlands. Our results affirm the importance of improving the characterization of forest structure when simulating surface albedo and associated climate effects.
Authors
Irene Biancarosa Ikram Belghit Christian Guido Bruckner Nina Sylvia Liland Rune Waagbø Heidi Amlund Svenja Heesch Erik-Jan LockAbstract
BACKGROUND: In the past few years, much effort has been invested into developing a new blue economy based on harvesting, cultivating and processing marine macroalgae in Norway. Macroalgae have high potential for a wide range of applications, e.g. as source of pharmaceuticals, production of biofuels or as food and feed. However, data on the chemical composition of macroalgae from Norwegian waters are scant. This study was designed to characterize the chemical composition of 21 algal species. Both macro- and micronutrients were analysed. Concentrations of heavy metals and the metalloid arsenic in the algae were also quantified. RESULTS: The results confirm that marine macroalgae contain nutrients which are relevant for both human and animal nutrition, the concentrations whereof are highly dependent on species. Although heavy metals and arsenic were detected in the algae studied, concentrations were mostly below maximum allowed levels set by food and feed legislation in the EU. CONCLUSION: This study provides chemical data on a wide range of algal species covering the three taxonomic groups (brown, red and green algae) and discusses both benefits of and potential limitations to their use for food and feed purposes.
Abstract
The apple fruit moth Argyresthia conjugella (Lepidoptera, Yponomeutidae) is a seed predator of rowan (Sorbus aucuparia) and is distributed in Europe and Asia. In Fennoscandia (Finland, Norway and Sweden), rowan fruit production is low every 2–4 years, and apple (Malus domestica) functions as an alternative host, resulting in economic loss in apple crops in inter-mast years. We have used Illumina MiSeq sequencing to identify a set of 19 novel tetra-nucleotide short tandem repeats (STRs) in Argyresthia conjugella. Such motifs are recommended for genetic monitoring, which may help to determine the eco-evolutionary processes acting on this pest insect. The 19 STRs were optimized and amplified into five multiplex PCR reactions. We tested individuals collected from Norway and Sweden (n = 64), and detected very high genetic variation (average 13.6 alleles, He = 0.75) compared to most other Lepidoptera species studied so far. Spatial genetic differentiation was low and gene flow was high in the test populations, although two non-spatial clusters could be detected. We conclude that this set of genetic markers may be a useful resource for population genetic monitoring of this economical important insect species.
Authors
Anne Linn Hykkerud Eivind Uleberg Espen Hansen Marieke Vervoort Jørgen A.B. Mølmann Inger MartinussenAbstract
Cloudberry (Rubus chamaemorus L.) is a wild perennial shrub growing on peatland with a circumpolar distribution. The combined berries have a high polyphenol content comprised primarily of ellagitannins. A few commercial cultivars are available, and pre-breeding trials on clonal material from different geographical origins are in progress. The objective of this study was to investigate how the content of polyphenols of four different cloudberry cultivars were affected by harvesting time and climatic variations during a 3-year-period. Plants were grown outside in plots and berries were harvested when mature. Berries were analyzed for total polyphenols and total anthocyanins by spectrophotometer. Total ellagic acid was identified and quantified using HPLC-MS after hydrolysis of the extracts. Results showed that all measured parameters; total anthocyanins, total polyphenols and ellagic acid are strongly influenced by the genetic background. Although low anthocyanin contents were present in all genotypes, they were highly affected by climatic conditions, being highest at low temperatures. However, the content of ellagic acid was less affected by environmental conditions and showed little response to changing temperatures. In conclusion, ellagitannin content was the most dominating polyphenol group observed in this study and was affected by genetics and is therefore a good breeding criterion for increased health benefit of cloudberry.
Abstract
In temperate forests, red wood ants (Formica aquilonia) are considered ecosystem engineers affecting ecosystem properties and functions. Possible effects of F. aquilonia ants on species communities of invertebrates and plants were studied in the pine-dominated Geitaknottane forest reserve, Norway. Species richness of carabids, lichens and epiphytes (tree-living lichens and bryophytes) was negatively affected by ant mound density. Species of all groups, except for lichens and snails, were affected either positively or negatively by ant presence. Food availability and interference competition are plausible explanations of decreased species richness and negative species associations in carabids; while collecting, foraging and changed chemical environment may explain decreased species richness in lichens and epiphytes. Thirteen out of 15 plant and invertebrate species were weakly associated with ant mound density. Associations of only two species (Carabus violaceus and Drusilla canaliculata) were negative, while Pella humeralis and Agroeca proxima were associated positively and very strongly with ant mounds. Positive associations with ants of those invertebrates may be a response to excessive abundance of food and chemical mimicry.
Abstract
Lunch canteens and their salad bars are an important arena for sales and consumption of vegetables including herbs. One major Norwegian canteen operator had a turnover of more than seven thousand tons of fresh vegetables in 2016, with lettuce, tomato, potato, cucumber and bell pepper being the most important species. A typical lunch meal included about 260 g vegetables including potatoes. Vegetables used in 450 canteens were either green, yellow, orange, red, purple/dark or colorless, and consisted of pigments of chlorophylls, carotenoids, anthocyanins and betalains. The total pigment content in the 60 most abundant vegetables was calculated to be 14.5-28.3 mg 100 g-1 FW. Of all vegetables in the canteens, 60% were found to be green. The intake of chlorophyll through one lunch meal was estimated to be 46 mg. Lettuce was found to be the single most important source of chlorophylls as this species was consumed in high amounts and made up 20% of the vegetables in a lunch meal. Carotenoids was found in all colored vegetables except the purple/dark ones and an estimate revealed an intake of 15 mg total carotenoids from lunch vegetables. Tomato was found to be the most important carotenoid source representing 44% of the total intake. Due to high pigment concentrations and popularity of red beets in the salad bars, the intake of betalains through a lunch meal was estimated to be 3 mg, similar to the total intake of anthocyanins from vegetable species.
Authors
Pierrick Francois Denis Stévant Erlend Indergård Aðalheiður Ólafsdóttir Hélène Marfaing Wenche Merete Emblem Larssen Joël Fleurence Michael Roleda Turid Rustad Rasa Slizyte Tom Ståle NordtvedtAbstract
The effects of convective air-drying at 25, 40, and 70 °C and freeze-drying on the quality of the edible kelp Saccharina latissima to be used for food were investigated. Based on the analysis of the carbohydrate and amino acid profiles, as well as polyphenol, fucoxanthin, and ash contents, no significant differences were detected among sample groups, and air-drying up to 70 °C results in equally nutritious products at shorter processing times. Only the iodine content was found lower in freeze-dried compared to air-dried samples. The swelling capacity of the air-dried samples was significantly lower than in freeze-dried samples, particularly at high temperatures (40 and 70 °C), reflecting alteration of the physico-chemical properties of the seaweed during air-drying (attributed to product shrinkage) and reduced capacity of the final product to rehydrate. Structural differences between air-dried products at 25 and 70 °C may explain the differences in mouthfeel perception (dissolving rate) among the two sample groups observed during a sensory evaluation. Overall, the drying temperature within this range did not alter neither the aroma (i.e. odor) nor the flavor intensity of the product. In food applications where the product’s mechanical properties (e.g. porosity) are essential, freeze-drying, and to a lesser extent, air-drying at low temperatures, will result in higher quality products than air-drying at higher temperatures.
Abstract
Throughout history, man has strongly utilized and affected forest genetic resources in Europe. From an evolu-tionary perspective deforestation/fragmentation (→genetic drift), transfer of seeds and plants to new environ-ments (→mainly gene flow) and selective logging (→selection) are most relevant and have been particularlyaddressed in this review. In contrast to most conifers, broadleaved tree populations have been especially reducedby historic fragmentation, and consequently, the related genetic effects have been possibly more pronounced.Widespread wind-pollinated species with wind/animal dispersed seeds appear to be more resilient to frag-mentation than species with e.g. small geographic ranges and gravity dispersed seeds. In addition, naturallyfragmented populations in the range margins may be more vulnerable than central populations as conditions forgene flow are generally impaired in peripheral areas. Traits important for adaptation (e.g. bud burst, bud set) arecontrolled by many genes, and as a corollary of fragmentation such genes are lost at a low rate. Large scalecommercial translocation of seeds and plants for forestry purposes applies mostly to conifers and dates backabout two centuries. Although many translocations have been successful in a forestry perspective, exposure tonew selective regimes has sometimes challenged the adaptive limits of populations and caused setbacks or evendiebacks of populations, as well as influencing neighbouring populations with maladapted genes (e.g. Scots pine,maritime pine, larch). Many tree species have substantial plasticity in fitness-related traits, which is vital forsurvival and viability following translocations. Selective logging has been practiced in Europe over the last twocenturies and implies removal of superior trees with respect to growth and quality. Such traits are partly undergenetic control. Consequent removal of superior trees may therefore have negative effects on the remaining genepool, but this effect will also be counteracted by extensive gene flow. Although humans have strongly affectedEuropean forest trees over the last millennia, we argue that they are still resilient from an evolutionary perspective.
Authors
Patryk Czortek Jutta Kapfer Anna Delimat Amy Eycott John-Arvid Grytnes Anna Orczewska Halina Ratyńska Antoni Zięba Bogdan JaroszewiczAbstract
Mountain vegetation is often considered highly sensitive to climate and land-use changes due to steep environmental gradients determining local plant species composition. In this study we present plant species compositional shifts in the Tatra Mts over the past 90 years and discuss the potential drivers of the changes observed. Using historical vegetation studies of the region from 1927, we resurveyed 76 vegetation plots, recording the vascular flora of each plot using the same methodology as in the original survey. We used an indirect method to quantify plant species compositional shifts and to indicate which environmental gradients could be responsible for these shifts: by calculating shifts in estimated species optima as reflected in shifts in the ecological indicator values of co-occurring species. To find shifts in species composition, focusing on each vegetation type separately, we used ordination (DCA). The species optimum changed significantly for at least one of the tested environmental gradients for 26 of the 95 plant species tested; most of these species changed in terms of the moisture indicator value. We found that the strongest shifts in species composition were in mylonite grassland, snowbed and hygrophilous tall herb communities. Changes in precipitation and increase in temperature were found to most likely drive compositional shifts in vegetation resurveyed. It is likely that the combined effect of climate change and cessation of sheep grazing has driven a species composition shift in granite grasslands communities.
Abstract
Effects of annual versus biennial cropping with varying shoot densities on plant structure, berry yield and quality were studied in ‘Glen Ample’ raspberry over a period of four seasons (two cropping years). In the vegetative phase, primocane height and internode length were larger in the annual than in the biennial cropping system. These parameters as well as Botrytis infestation increased with increasing shoot density. In both cropping years, berry yields per unit area were about 20% higher in the biennial cropping system, whereas yields per shoot were not significantly different in the two systems. In both cropping systems, yields per shoot strongly declined with increasing shoot density, while yields per metre row increased slightly. Regardless of cropping system, yields per metre row did not increase with increasing shoot density beyond eight shoots per metre. The concentrations of dry matter, soluble solids, titratable acidity and ascorbic acid as well as the intensity of juice colour all declined with increasing shoot density. We conclude that under controlled shoot density conditions, there is little scope for biennial yield increases that fully compensates for the lost crops every second year. However, the system greatly facilitates berry harvest and eases plant disease pressure.
Abstract
The Norwegian area frame survey of land cover and outfield land resources (AR18X18), completed in 2014, provided unbiased statistics of land cover in Norway. The article reports the new statistics, discusses implications of the data set, and provides potential value in terms of research, management, and monitoring. A gridded sampling design for 1081 primary statistical units of 0.9 km2 at 18 km intervals was implemented in the survey. The plots were mapped in situ, aided by aerial photos, and all areas were coded following a vegetation type system. The results provide new insights into the cover and distribution of vegetation and land cover types. The statistic for mire and wetlands, which previously covered 5.8%, has since been corrected to 8.9%. The survey results can be used for environmental and agricultural management, and the data can be stratified for regional analyses. The survey data can also serve as training data for remote sensing and distribution modelling. Finally, the survey data can be used to calibrate vegetation perturbations in climate change research that focuses on atmospheric–vegetation feedback. The survey documented novel land cover statistics and revealed that the national cover of wetlands had previously been underestimated.
Authors
Solveig Marie Stubsjøen Kristin Sørheim Matteo Chincarini Jon Bohlin Emma Brunberg Boris Fuchs Rupert Palme Lise GrøvaAbstract
Tick-borne fever (TBF), caused by the bacterium Anaplasma phagocytophilum and transmitted by the tick Ixodes ricinus, has considerable consequences for animal welfare and economy in the sheep industry. Non-invasive, objective methods to quantify chronic stress are needed in order to evaluate the welfare impact of disease. The aim of this study was 1) to evaluate hair cortisol (HC) and hair cortisone (HCn) as biomarkers of chronic stress in sheep with TBF and 2) to test whether there was an association between the development of TBF and con- centrations of HC, HCn and faecal cortisol metabolites (FCM) and body weight. The experiment took place in an area with a high prevalence of TBF, and thirty lambs were used in the study. Wool samples were collected in Week 0, in Week 3 (before turn out on homeland spring pasture), in Week 6 (before turn out on summer rangeland pasture) and at the end of the summer (Week 15). Faecal samples were collected every week (ie. Week 0–6 and Week 15). Symptoms of TBF developed in 15 lambs, of which all recovered from the disease after treatment with antibiotics. HC levels decreased progressively, and significantly, between Week 0, 3, 6 and 15 (p < 0.001), while HCn only decreased from Week 0 to Week 3 (p < 0.001) and then remained stable between Week 3–15. FCM increased between Week 0 and 5 (p = 0.027), and a significant association was found between increased FCM levels in Week 5 and lambs developing clinical signs of TBF (p = 0.022). We also found an association between lambs developing clinical signs of TBF and elevated HCn levels in Week 6 (p = 0.013). A slightly lower weight gain at later time points (Week 6 and 15) were found in the affected lambs compared to clinically healthy lambs. Our results indicate local production and/or metabolism of glucocorticoids in the hair follicles. This study strengthens our previous finding of a potential merit of hair cortisone as a biomarker of chronic stress in sheep.
Authors
E. Stefanczyk M. Brylinska May Bente Brurberg Ragnhild Nærstad Abdelhameed Elameen S. Sobkowiak J. SliwkaAbstract
The oomycete Phytophthora infestans, the cause of late blight, is one of the most important potato pathogens. During infection, it secretes effector proteins that manipulate host cell function, thus contributing to pathogenicity. This study examines sequence differentiation of two P. infestans effectors from 91 isolates collected in Poland and Norway and five reference isolates. A gene encoding the Avr-vnt1 effector, recognized by the potato Rpi-phu1 resistance gene product, is conserved. In contrast, the second effector, AvrSmira1 recognized by Rpi-Smira1, is highly diverse. Both effectors contain positively selected amino acids. A majority of the polymorphisms and all selected sites are located in the effector C-terminal region, which is responsible for their function inside host cells. Hence it is concluded that they are associated with a response to diversified target protein or recognition avoidance. Diversification of the AvrSmira1 effector sequences, which existed prior to the large-scale cultivation of plants containing the Rpi-Smira1 gene, may reduce the predicted durability of resistance provided by this gene. Although no isolates virulent to plants with the Rpi-phu1 gene were found, the corresponding Avr-vnt1 effector has undergone selection, providing evidence for an ongoing ‘arms race’ between the host and pathogen. Both genes remain valuable components for resistance gene pyramiding.
Authors
Van Minh Dang Stephen Joseph Huu Tap Van Thi Lan Anh Mai Thi Minh Hoa Duong Simon Weldon Paul Munroe David Mitchell Sarasadat TaherymoosaviAbstract
Heavy metal contamination of crop lands surrounding mines in North Vietnam is a major environmental issue for both farmers and the population as a whole. Technology for the production of biochar at a village and household level has been successfully introduced into Vietnamese villages. This study was undertaken to determine if rice straw biochar produced in simple drum ovens could remediate contaminated land. Tests were also carried out to determine if biochar and apatite mixed together could be more effective than biochar alone. Incubation trials were carried out over 90 days in pots to determine the total changes in exchangeable Cd, Pb and Zn. Detailed tests were carried out to determine the mechanisms that bound the heavy metals to the biochar. It was found that biochar at 5% (BC5) and the mixture of biochar and apatite at 3% (BCA3) resulted in the greatest reduction of exchangeable forms of Cd, Pb and Zn. The increase in soil pH caused by adding biochar and apatite created more negative charge on the soil surface that promoted Pb, Zn and Cd adsorption. Heavy metals were mainly bound in the organic, Fe/Mn and carbonate fractions of the biochar and the mixture of biochar and apatite by either ion exchange, adsorption, dissolution/precipitation and through substitution of cations in large organic molecules.
Abstract
Modified atmosphere packaging (MAP) may inhibit undesirable quality changes of fruit and vegetables. The aim of this experiment was to evaluate the effect of MAP on selected quality parameters for sweet cherries (Prunus avium L.) stored at simulated distribution chain temperatures. ‘Lapins’ sweet cherries with maturity grade 4-5 and 6-7 were packaged in macroperforated polyethylene “carry bags” (control) and in trays wrapped in a laser perforated film giving passive modified atmosphere (MAP). After packaging, the cherries were stored at 4°C for 5 days and thereafter for 3 days at 4°C (chill) or 20°C (retail) simulating storage at chill or room temperature in the grocery stores. Headspace gas atmosphere in the MA packages, fruit quality, weight loss and amount of fungal fruit decay and other decays were recorded after 1, 5 and 8 days of storage. The gas atmosphere in MA packages was approximately 18% O2 and 4% CO2 at 4°C and between 6-9% O2 and 12-14% CO2 at 20°C. The weight loss was negligible in the MA packages at both storage conditions, whereas the cherries in carry bags showed a weight loss from 1 to 4%. The stem colour was significantly browner in the carry bags compared to the MA packages after 8 days of retail storage. Fungal decay was below 0.5% for both maturity grades stored at chill conditions for 8 days. At retail conditions, 4 and 6% decay was detected for maturity grade 4-5 in MA-packages and carry bags, respectively. For maturity grade 6- 7, the MA-packages had 9% decay and the carry bags 7%. The overall picture was that MA packaging for sweet cherries better maintained the fruit quality than the carry bags during the storage period of 8 days at two simulated retail conditions.
Abstract
Large areas of farmland are abandoned in Norway, which for various reasons are regarded as undesirable. Loss of farmlandmay have negative implications for biodiversity and ecosystem function and food production potential. The objectives of this study were to assess forage mass production and utilization, botanical composition, lamb performance, and grazing distribution pattern when reintroducing livestock grazing to an abandoned grassland. The study area was located in Central Norway, unmanaged for 12 years. Sheep grazed the area for 10 weeks in 2013 and 4 weeks in spring and autumn, respectively, in 2014 and 2015. During the summer of 2014 and 2015, the area was subjected to the following replicated treatments: (1) No grazing, (2) grazing with heifers, and (3) grazing with ewes and their offspring. The stocking rate was similar in the grazed treatments. Forage biomass production and animal intake were estimated using grazing exclosure cages and botanical composition by visual assessment. Effect on lamb performance was evaluated by live weight gain and slaughter traits in sheep subjected to three treatments: (1) Common farm procedure with summer range pasturing, (2) spring grazing period extended by 1 month on the abandoned grassland before summer range pasturing, and (3) spring and summer grazing on the abandoned grassland. Grazing distribution patterns were studied using GPS position collars on ewes. Total annual biomass production was on average 72% higher with summer grazing than without. Annual consumption and utilization was on average 218 g DM/m2 and 70% when summer grazed, and 25 g DM/m2 and 18% without grazing, respectively. Botanical composition did not differ between treatments. Live weight gain was higher in lambs subjected to an extended spring grazing period (255 g/d) compared to common farm practice (228 g/d) and spring and summer grazing on the abandoned grassland (203 g/d), and carcass value was 14% higher in lambs on extended spring grazing compared to common farm practice. In autumn, sheep preferred to graze areas grazed by sheep during summer. Re-introduction of grazing stimulated forage production, and extended spring grazing improved performance in lambs. This study has quantified the value of abandoned grassland as a feed resource.
Authors
Jan Pisek Henning Buddenbaum Fernando Camacho Joachim Hill Jennifer L.R. Jensen Holger Lange Zhili Liu Arndt Piayda Yonghua Qu Olivier Roupsard Shawn P. Serbin Svein Solberg Oliver Sonnentag Anne Thimonier Francesco VuoloAbstract
Clumping index (CI) is a measure of foliage aggregation relative to a random distribution of leaves in space. The CI can help with estimating fractions of sunlit and shaded leaves for a given leaf area index (LAI) value. Both the CI and LAI can be obtained from global Earth Observation data from sensors such as the Moderate Resolution Imaging Spectrometer (MODIS). Here, the synergy between a MODIS-based CI and a MODIS LAI product is examined using the theory of spectral invariants, also referred to as photon recollision probability (‘p-theory’), along with raw LAI-2000/2200 Plant Canopy Analyzer data from 75 sites distributed across a range of plant functional types. The p-theory describes the probability (p-value) that a photon, having intercepted an element in the canopy, will recollide with another canopy element rather than escape the canopy. We show that empirically-based CI maps can be integrated with the MODIS LAI product. Our results indicate that it is feasible to derive approximate p-values for any location solely from Earth Observation data. This approximation is relevant for future applications of the photon recollision probability concept for global and local monitoring of vegetation using Earth Observation data.
Abstract
The Norwegian per capita sales of wine have more than doubled over the past 20 years, while the sales of sprits and beer have declined. These changes are likely to be the effect of changes in economic, demographic, and attitudinal factors as well as the availability of wine. We estimated age-period-cohort (APC) logit models using data from a large repeated cross-sectional survey over the period 1991–2015. The estimation results indicate substantial effects of the APC variables as well as income, availability, and attitudes. The model was used to simulate wine consumption over the life cycle in different birth cohorts. The simulation results indicate that wine consumption frequency increases by age, and younger cohorts are expected to increase their consumption frequencies more than older cohorts, which suggests an increased wine consumption over time.
Abstract
This paper aimed to investigate the genetic structure (GS) of Scots pine in the northern area of its distribution range by means of seven neutral nuclear microsatellite markers. In particular, the postglacial recolonization of these areas and possible different adaptation patterns in distinct refugia were studied. The GS and diversity were assessed with seven pairs of neutral nuclear microsatellite primers. A high genetic diversity was found in the Scots pine material tested, along with a shallow GS. This pattern is typical for recolonized areas and species with large population sizes, which are connected by pollen-mediated gene flow. A STRUCTURE analysis found two genetic groups to be the most likely, one south-eastern and one north-western group that meet in Fennoscandia. This indicates that Scots pine recolonization of Fennoscandia might have taken place from two different directions (south-west and north-east). Scots pine that recolonized the area originated in at least two different refugia during the last glacial maximum. The glacial survival in distinct refugia can have led to different adaptation patterns and growth optima in the different groups as reflected in the formation of latewood content, where lineage was a significant influencing factor.
Authors
Annika Kangas Rasmus Astrup Johannes Breidenbach Jonas Fridman Terje Gobakken Kari T. Korhonen Matti Maltamo Mats Nilsson Thomas Nord-Larsen Erik Næsset Håkan OlssonAbstract
The Nordic countries have long traditions in forest inventory and remote sensing (RS). In sample-based national forest inventories (NFIs), utilization of aerial photographs started during the 1960s, satellite images during the 1980s, laser scanning during the 2000s, and photogrammetric point clouds during the 2010s. In forest management inventories (FMI), utilization of aerial photos started during the 1940s and laser scanning during the 2000s. However, so far, RS has mostly been used for map production and research rather than for estimation of regional parameters or inference on their accuracy. In recent years, the RS technology has been developing very fast. At the same time, the needs for information are constantly increasing. New technologies have created possibilities for cost-efficient production of accurate, large area forest data sets, which also will change the way forest inventories are done in the future. In this study, we analyse the state-of-the-art both in the NFIs and FMIs in the Nordic countries. We identify the benefits and drawbacks of different RS materials and data acquisition approaches with different user perspectives. Based on the analysis, we identify the needs for further development and emerging research questions. We also discuss alternatives for ownership of the data and cost-sharing between different actors in the field.
Abstract
The population structure of cloudberry (Rubus chamaemorus L.), collected from Krkonose Mountains (the Czech Republic), continental Norway and Spitsbergen, was examinedusing microsatellite analyses (SSR). Among 184 individuals, 162 different genotypeswere identified. The overall unbiased gene diversity was high (̂h=0.463). A high level of genetic differentiation among populations (FST = 0.45; p < .01) indicated restricted gene flow between populations. Using a Bayesian approach, six clusters were found which represented the genetic structure of the studied cloudberry populations. The value of correlation index between genetic and geographical distances (r = .44) indicates that gene flow, even over a long distance, could exist. An exact test of population differentiation showed that Rubus chamaemorus populations from regions (Krkonose Mountains,continental Norway and Spitsbergen) are differentiated although some individuals within populations share common alleles even among regions. These results were confirmed by AMOVA, where the highest level of diversity was found within populations(70.8%). There was no difference between 87 pairs of populations (18.7%) mostly within cloudberry populations from continental Norway and from Spitsbergen. Based on obtained results, it is possible to conclude that Czech and Norwegian cloudberry popula-tions are undergoing differentiation, which preserves unique allele compositions most likely from original populations during the last glaciation period. This knowledge will be important for the creation and continuation of in situ and ex situ conservation of cloud-berry populations within these areas.
Abstract
This paper addresses the endogeneity of inputs and output (which is mostly ignored in the stochastic frontier (SF) literature) in the SF panel data model under the behavioural assumption that firms maximize returns to the outlay. We consider a four component SF panel data model in which the four components are: firms' latent heterogeneity, persistent inefficiency, transient inefficiency and random shocks. Second, we include determinants in transient inefficiency. Finally, to avoid the impact of distributional assumptions in estimating the technology parameters, we apply a multi-step estimation strategy to an unbalanced panel dataset from Norwegian crop-producing farms observed from 1993 to 2014. Distributional assumptions are made in second and third steps to predict both persistent and transient inefficiency, and their marginal effects. Keywords Efficiency; Endogeneity; Returns to the outlay; Panel data
Authors
Merethe Kleiven Lisa Magdalena Rossbach Julian Alberto Gallego-Urrea Dag Anders Brede Deborah H Oughton Claire CoutrisAbstract
Using Caenorhabditis elegans as a model organism, this study addresses the potential linkage between toxicity of NM300K Ag nanoparticles (AgNPs), their particle size distribution and the presence of dissolved Ag in the test media. Of the three endpoints assessed (growth, fertility and reproduction), reproduction was the most sensitive, with 50% effect concentration (EC50) ranging from 0.26-0.84 mg Ag L-1 and 0.08-0.11 mg Ag L-1 for NM300K and AgNO3, respectively. Silver uptake by C. elegans was similar for both forms of Ag, while bioaccumulation was higher in AgNO3 exposure. The observed differences in toxicity between NM300K and AgNO3 did not correlate to bioaccumulated Ag, which suggests the toxicity to be a function of the type of exposing agent (AgNPs vs AgNO3) and their mode of action. Before addition of the food source, E. coli, size fractionation revealed that dissolved Ag comprised 13-90 % and 4-8 % of total Ag in the AgNO3 and NM300K treatments, respectively. No dissolved Ag was detectable in the actual test media, due to immediate Ag adsorption to bacteria. Results from the current study highlight that information on behavior and characterization of exposure conditions is essential for nanotoxicity studies.
Abstract
Questions : Land-cover maps are used for nature management, but can they be trusted? This study addresses three questions: (1) what is the magnitude of between field worker inconsistencies in land-cover maps and what may cause such inconsistencies; (2) in which ways and to what extent do spatial scale and mapping system influence inconsistencies between maps; and (3) are some biomes mapped more consistently than others, and if so, why? Location : Gravfjellet, Øystre Slidre municipality, southern Norway. Methods : Two different mapping systems, designed for mapping at different spatial scales, were used for parallel mapping by three different field workers, giving a total of six maps for the study area. Spatial consistency of the resulting maps was compared at two hierarchical levels for both systems. Results : The average pair-wise spatial consistency at the highest hierarchical level was 83% for both systems, while the average pair-wise spatial consistency at the lowest hierarchical level was 60.3% for the coarse system and 43.8% for the detailed system. Inconsistencies between maps were partly caused by the use of different land- cover units and partly by spatial displacement. Conclusions : Field workers made different maps despite using the same mapping systems, materials and methods. The differences were larger at lower hierarchical levels in the mapping systems and increased strongly with system complexity. Consistency among field workers should be estimated as a standard quality indicator in all field-based mapping programmes.
Authors
Louise Eriksson Johanna Boberg Thomas L. Cech Tamara Corcobado Marie-Laure Desprez-Loustau Ari Hietala Marilia Horta Jung Thomas Jung Hatice Tugba Dogmus Lehtijarvi Funda Oskay Slavtcho Slavov Halvor Solheim Jan Stenlid Jonas OlivaAbstract
Political action can reduce introductions of diseases caused by invasive forest pathogens (IPs) and public support is important for effective prevention. The public’s awareness of IP problems and the acceptability of policies aiming to combat these pathogens were surveyed in nine European countries (N = 3469). Although awareness of specific diseases (e.g., ash dieback) varied, problem awareness and policy acceptability were similar across countries. The public was positive towards policies for informational measures and stricter standards for plant production, but less positive towards restricting public access to protected areas. Multilevel models, including individual and country level variables, revealed that media exposure was positively associated with awareness of IP problems, and strengthened the link between problem awareness and policy acceptability. Results suggest that learning about IPs through the media and recognizing the associated problems increase policy acceptability. Overall, the study elaborates on the anthropogenic dimension of diseases caused by IPs.
Authors
James Johnson Elisabeth Graf Pannatier Stefano Carnicelli Guia Cecchini Nicholas Clarke Nathalie Cools Karin Hansen Henning Meesenburg Tiina M. Nieminen Gunilla Pihl-Karlsson Hugues Titeux Elena Vanguelova Arne Verstraeten Lars Vesterdal Peter Waldner Mathieu JonardAbstract
Acid deposition arising from sulphur (S) and nitrogen (N) emissions from fossil fuel combustion and agriculture has contributed to the acidification of terrestrial ecosys- tems in many regions globally. However, in Europe and North America, S deposition has greatly decreased in recent decades due to emissions controls. In this study, we assessed the response of soil solution chemistry in mineral horizons of European forests to these changes. Trends in pH, acid neutralizing capacity (ANC), major ions, total aluminium (Al tot ) and dissolved organic carbon were determined for the period 1995–2012. Plots with at least 10 years of observations from the ICP Forests moni- toring network were used. Trends were assessed for the upper mineral soil (10– 20 cm, 104 plots) and subsoil (40–80 cm, 162 plots). There was a large decrease in the concentration of sulphate (SO 2 4 ) in soil solution; over a 10-year period (2000– 2010), SO 2 4 decreased by 52% at 10–20 cm and 40% at 40–80 cm. Nitrate was unchanged at 10–20 cm but decreased at 40–80 cm. The decrease in acid anions was accompanied by a large and significant decrease in the concentration of the nutrient base cations: calcium, magnesium and potassium (Bc = Ca 2+ + Mg 2+ + K + ) and Al tot over the entire dataset. The response of soil solution acidity was nonuni- form. At 10–20 cm, ANC increased in acid-sensitive soils (base saturation ≤10%) indicating a recovery, but ANC decreased in soils with base saturation >10%. At 40–80 cm, ANC remained unchanged in acid-sensitive soils (base saturation ≤20%, pH CaCl 2 ≤ 4.5) and decreased in better-buffered soils (base saturation >20%, pH CaCl 2 > 4.5). In addition, the molar ratio of Bc to Al tot either did not change or decreased. The results suggest a long-time lag between emission abatement and changes in soil solution acidity and underline the importance of long-term monitor- ing in evaluating ecosystem response to decreases in deposition.
Authors
Kine Svensson Oda Marie Kjørlaug Matthew J. Higgins Roar Linjordet Svein Jarle HornAbstract
Post-anaerobic digestion (PAD) treatment technologies have been suggested for anaerobic digestion (AD) to improve process efficiency and assure hygenization of organic waste. Because AD reduces the amount of organic waste, PAD can be applied to a much smaller volume of waste compared to pre-digestion treatment, thereby improving efficiency. In this study, dewatered digestate cakes from two different AD plants were thermally hydrolyzed and dewatered, and the liquid fraction was recirculated to a semi-continuous AD reactor. The thermal hydrolysis was more efficient in relation to methane yields and extent of dewaterability for the cake from a plant treating waste activated sludge, than the cake from a plant treating source separated food waste (SSFW). Temperatures above 165 °C yielded the best results. Post-treatment improved volumetric methane yields by 7% and the COD-reduction increased from 68% to 74% in a mesophilic (37 °C) semi-continuous system despite lowering the solid retention time (from 17 to 14 days) compared to a conventional system with pre-treatment of feed substrates at 70 °C. Results from thermogravimetric analysis showed an expected increase in maximum TS content of dewatered digestate cake from 34% up to 46% for the SSFW digestate cake, and from 17% up to 43% in the sludge digestate cake, after the PAD thermal hydrolysis process (PAD-THP). The increased dewatering alone accounts for a reduction in wet mass of cake leaving the plant of 60% in the case of sludge digestate cake. Additionaly, the increased VS-reduction will contribute to further reduce the mass of wet cake.
Abstract
Forestry in coastal Norway has traditionally been a marginal activity with a low annual harvest rate. However, the region is now faced with large areas of spruce plantations that will reach harvest maturity within the next 25 years. Due to the poor infrastructure in the region, the current challenge is to harvest the maturing spruce plantations at an acceptable cost. Hence, there is considerable interest both from the forest sector and politicians to invest in infrastructure that can provide the basis for profitable forest sector development in coastal Norway. This paper presents a mathematical optimization model for timber transportation from stump to industry. The main decision variables are location of quays, upgrade of public road links, the length of new forest roads, and when the investments should happen. The main objective is to provide decision support for prioritization of infrastructure investments. The optimization model is combined with a dynamical forest resource model, providing details on available volumes and costs. A case study for coastal Norway is presented and solved to optimality. The instance includes 10 counties comprising more than 200 municipalities with forest resources, 53 possible new quays for timber export and 916 public road links that also can be upgraded. Compared with a no investment case, the optimal solution improved the objective by 23%. The study shows that consistent, informative and good analyses can be performed to evaluate trade-offs, prioritization, time and order of investment, and cost saving potentials of infrastructure investments in the forest industry. The solution seems reasonable based on present infrastructure and state of the forest.
Authors
Jørn Henrik Sønstebø Mari Mette Tollefsrud Tor Myking Arne Steffenrem Anne Eskild Nilsen Øyvind Meland Edvardsen O. Ragnar Johnskås Yousry A. El-KassabyAbstract
Seed from orchards, established from breeding programs, often dominate the planting stock in economically important tree species, such as Norway spruce. The genetic diversity in seed orchards’ crops depends on effective population size which in turn is affected by many factors such as: number of parents in the orchard, seed orchards’ design, fecundity, and pollen contamination. Even though seed orchards’ seed is extensively used over large regions, very few studies have addressed how well their crops reflect the genetic diversity present in the regions where they are planted. Here we have investigated the genetic diversity (by means of 11 microsatellites) of two Norway spruce seed orchard populations with different number of parents (60 and 25) and compared this with seed crops collected in the semi natural forest and natural unmanaged populations. We found that the ratio between the effective population size (N e ) and actual number of parents (N) varied between 0.60 and 0.76 in the orchards’ seedlots. A reduction in genetic diversity (mainly allelic richness) was detected in a few seedlots, mainly where the number of parents was low. Our results also show that pollen contamination play an important role in maintaining the genetic diversity in orchards’ seedlots, particularly when the number of parents is low. The population genetic structure among seed orhcards and natural populations is shallow suggesting that re- generation with seed from current seed orchards will have limited effect on the overall genetic diversity.
Abstract
The aim of this study was to contribute to the development of pelleted compound recycling fertilizerswith favourable handling and spreading characteristics and balanced nutrient ratios by combiningnitrogen (N)- and phosphorus (P)-rich waste resources (meat bone meal, fish sludge or food waste)with potassium (K)-rich bottom wood ash. Pelleted compound recycling fertilizers with gooddurability and low dusting tendency were produced by roll-pelleting preheated waste resources at asuitable moisture content. However, the nutrient ratios in the final products were insufficientlybalanced, with too low N concentrations relative to P and K to meet crop demands. In a bioassayusing barley ( Hordeum vulgare) and a nutrient-deficient sand/peat mixture, the relative agronomiceffectiveness (RAE) of pelleted compound recycling fertilizers and reference recycling fertilizers was22–42% of that of mineral compound fertilizer. Growth limitation was due to reduced N availability(mineral fertilizer equivalent - MFE = 35–57%) or reduced P availability (MFE = 20–115%), with thegreatest P fertilizer value obtained for digestate based on dairy manure and fish sludge. Availability ofK in bottom wood ash was masked by the experimental soil.
Abstract
Productivity of a mechanized P. patula cut-to-length harvesting operation was estimated and modelled using two methods of data collection: manual time study and follow-up study using StanForD stem files. The objective of the study was to compare the productivity models derived using these two methods to test for equivalence. Manual time studies were completed on four different machines and their operators. Two Ponsse Bear harvesters fitted with H8 heads, and two Ponsse Beaver harvesters, fitted with H6 heads, were included. All machines were equipped with Ponsse Opti2 information system. All four operators had approximately 1 year of experience working with their respective machines. The four machines worked in separate four-tree-wide harvesting corridors, and they each harvested 200 trees. Individual tree diameter at breast height (DBH), and height measurements were made manually. Subsequently, data on the trees in each study were extracted from the StanForD stem reports from each of the harvesters. Cycle times in the stem reports were determined based on the difference between consecutive harvest timestamps. The two methods were compared in terms of their abilities to estimate equivalent measures for tree DBH, volume, and productivity. In all four cases, significant differences were found between the DBH and volume measures derived using the two methods. Subsequently, the volume measures from the manual methods were used as the basis for productivity calculations. Results of the productivity comparisons found no significant differences between the models developed from the two methods. These results suggest that equivalent productivity models can be developed in terms of time using either method, however volume discrepancies indicate a need to reconcile bark and volume functions with the high variability experienced in the country.
Abstract
We compare the food values in the USA and Norway using the best–worst scaling approach. The food values examined are aimed at capturing the main issues related to food consumption such as naturalness, taste, price, safety, convenience, nutrition,novelty, origin, fairness, appearance, environmental impact and animal welfare. Results show that respondents in both countries have mostly similar food values,with safety being the most important value; while convenience and novelty are the least important values. Specifically, US respondents consider price more important and naturalness less important than Norwegian respondents.
Authors
Camilo Chiang Oda Toresdatter Aas Marianne Rindedal Jetmundsen YeonKyeong Lee Sissel Torre Inger Sundheim Fløistad Jorunn Elisabeth OlsenAbstract
Subalpine fir (Abies lasiocarpa (Hooker) Nuttall), which is native to western North America, is of considerable interest for Christmas tree production in northern Europe. Seedlings are usually grown from seeds under combined nursery greenhouse/outdoors conditions, but commonly show early growth cessation in the nursery, resulting in small plants for field transplanting. This increases the production time and makes the seedlings vulnerable to stressors at the planting site. Day extension with far-red (FR) light was shown to enhance elongation and delay bud set in seedlings of some woody species, but such information is limited for Abies. Here, we investigated the effects of day extension with FR, red (R), different R:FR-ratios or blue (B) light from light emitting diodes on subalpine fir seedlings grown at different temperatures. Day extension with FR or combined R-FR light, in contrast to R or B light, increased shoot elongation significantly as compared to short days without day extension, often with more growth at 18 ◦C than 24 ◦C. The FR treatments delayed terminal bud development, although bud set was not completely prevented. These results demonstrate that larger seedlings of subalpine fir seedlings for Christmas tree production can be obtained by employing day extension with FR or combined R:FR light, preferably under cool temperature.
Authors
Pieternella C. Luttikhuizen Freek H. M. van den Heuvel Celine Rebours Harry J. Witte Judith D. L. van Bleijswijk Klaas TimmermansAbstract
Kelp aquaculture is globally developing steadily as human food source, along with other applications. One of the newer crop species is Saccharina latissima, a northern hemisphere kelp inhabiting temperate to arctic rocky shores. To protect and docu-ment its natural genetic variation at the onset of this novel aquaculture, as well as increase knowledge on its taxonomy and phylogeography, we collected new geneticdata, both nuclear and mitochondrial, and combined it with previous knowledge to estimate genetic connectivity and infer colonization history. Isolation-with- migration coalescent analyses demonstrate that gene flow among the sampled locations is vir -tually nonexistent. An updated scenario for the origin and colonization history of S. latissima is developed as follows: We propose that the species (or species complex) originated in the northwest Pacific, crossed to the northeast Pacific in the Miocene, and then crossed the Bering Strait after its opening ~5.5 Ma into the Arctic and northeast Atlantic. It subsequently crossed the Atlantic from east to west. During the Pleistocene, it was compressed in the south with evidence for northern refugia in Europe. Postglacial recolonization led to secondary contact in the Canadian Arctic. Saccharina cichorioides is shown to probably belong to the S. latissima species com -plex and to derive from ancestral populations in the Asian North Pacific. Our novel approach of comparing inferred gene flow based on coalescent analysis versus Wright’s island model suggests that equilibrium levels of differentiation have not yet been reached in Europe and, hence, that genetic differentiation is expected to in -crease further if populations are left undisturbed.
Abstract
Four lab scale biogas reactors fed with a substrate composition of ensiled fish waste and manure fixed at 13 and 87 vol %, respectively, were operated with HRTs of 20, 25, 30 and 40 days. Biogas process performance and stability were evaluated with regard to CH4 yields, NH4+ accumulation and abundance of NH4+-tolerant microorganisms. Process performance in the reactors operated at different HRTs were compared to process performance in reactors operated with constant HRT, fed with increased ratios of fish waste. The process performance and microbial dynamics were stable in reactors operated at constant amount of fish waste in the feed and with different HRTs. In the reactors added elevated ratios of fish waste, the concentration of NH4+ and abundance of NH4+-tolerant acetate oxidizing bacteria increased. The biogas process failed in these reactors simultaneously with an observed shift in microbial composition. In particular, the bacterium Tepidanaerobacter Acetatoxydans seemed to affect the biogas process stability. The hydrogenotrophic Methanomicrobiales increased in abundance in response to higher fish waste loading and NH4+ concentrations. This study showed that at a loading of 13% fish waste, it is possible to decrease the HRT from 30 to 20 days without markedly inhibiting the process stability.
Authors
Frans-Jan W. Parmentier Daniel Rasse Magnus Lund Jarle W. Bjerke Bert G. Drake Simon Weldon Hans Tømmervik Georg Heinrich HansenAbstract
Extreme winter events that damage vegetation are considered an important climatic cause of arctic browning—a reversal of the greening trend of the region—and possibly reduce the carbon uptake of northern ecosystems. Confirmation of a reduction in CO2 uptake due to winter damage, however, remains elusive due to a lack of flux measurements from affected ecosystems. In this study, we report eddy covariance fluxes of CO2 from a peatland in northern Norway and show that vegetation CO2 uptake was delayed and reduced in the summer of 2014 following an extreme winter event earlier that year. Strong frost in the absence of a protective snow cover—its combined intensity unprecedented in the local climate record—caused severe dieback of the dwarf shrub species Calluna vulgaris and Empetrum nigrum. Similar vegetation damage was reported at the time along ~1000 km of coastal Norway, showing the widespread impact of this event. Our results indicate that gross primary production (GPP) exhibited a delayed response to temperature following snowmelt. From snowmelt up to the peak of summer, this reduced carbon uptake by 14 (0–24) g C m−2 (~12% of GPP in that period)—similar to the effect of interannual variations in summer weather. Concurrently, remotely-sensed NDVI dropped to the lowest level in more than a decade. However, bulk photosynthesis was eventually stimulated by the warm and sunny summer, raising total GPP. Species other than the vulnerable shrubs were probably resilient to the extreme winter event. The warm summer also increased ecosystem respiration, which limited net carbon uptake. This study shows that damage from a single extreme winter event can have an ecosystem-wide impact on CO2 uptake, and highlights the importance of including winter-induced shrub damage in terrestrial ecosystem models to accurately predict trends in vegetation productivity and carbon sequestration in the Arctic and sub-Arctic.
Abstract
Effects of climatic factors and material properties on the development of surface mould growth on wooden claddings were investigated in a laboratory experiment. Specimens of aspen (Populus tremula), Siberian larch (Larix Sibirica), American white oak (Querqus alba), Scots pine (Pinus sylvestris), Norway spruce (Picea abies) and thermally modified pine were incubated in eight climatic chambers at specified wetting periods (2 or 4 h per day), relative humidity (58–86%) and temperature conditions (10–27°C). Surface mould growth was assessed weekly for 13 weeks, and the results were evaluated statistically using Generalized Estimating Equations logistic regression models. All tested climatic factors had significant effects on the mould growth, and there were significant differences between the materials. The ranking of the materials varied with temperature and over time. Aspen, pine sapwood and oak were overall most susceptible to mould growth, and thermally modified pine least susceptible. There were significant differences between sapwood and heartwood for pine and spruce. The effect of density was tested on the spruce heartwood material, but was not found to be significant. The results can be used to further develop prediction models for mould growth on wooden claddings.
Abstract
This article aims to explore differences in motivation for and actual use of allotment gardens. Results from questionnaire surveys and semistructured interviews in two Norwegian and one Dutch garden show that growing vegetables and consuming the harvest is a fundamental part of gardening. The same is true for the social element—meeting and talking to other gardeners, and feeling as part of a community. Although gardeners with different socioeconomic backgrounds experience gardening to some extent similarly, access to an allotment seems more important for gardeners with disadvantaged personal backgrounds: both their diets and their social networks rely more on, and benefit more from, their allotments. This underlines the importance of providing easy access to gardening opportunities for all urban residents, and disadvantaged groups in particular. Public officers and policy makers should consider this when deciding upon new gardening sites or public investments in urban food gardens.
Authors
Victor Rueda-Ayala Hella Ellen Ahrends Stefan Siebert Thomas Gaiser Hubert Hüging Frank EwertAbstract
Impacts of nutrient supply and different cultivars (genotypes) on actual yield levels have been studied before, but the long-term response of yield trends is hardly known. We present the effects of 24 different fertilizer treatments on long-term yield trends (1953–2009) of winter wheat, winter rye, sugar beet and potato, with improved cultivars changing gradually over time. Data was obtained from the crop rotation within the long-term fertilization experiment at Dikopshof, Germany. Yield trends were derived as the slope regression estimates between adjusted yield means and polynomials of the first year of cultivation of each tested cultivar, when tested for more than two years. A linear trend fitted best all data and crops. Yields in highly fertilized treatments increased linearly, exceeding 0.08 t ha−1 a−1 for both, winter wheat and winter rye, and ≥0.30 and ≥0.20 t ha−1 a−1 for sugar beet and potato fresh matter yields. Yield trends of winter cereals and sugar beet increased over time at N rates ≥40 kg ha−1 a−1, being 0.04–0.10 t ha−1 a−1 for cereals and 0.26–0.34 t ha−1 a−1 for sugar beet, although N rates >80 kg ha−1 a−1 produced a stronger effect. Nitrogen was the most influential nutrient for realisation of the genetic yield potential. Additional supply of P and K had an effect on yield trends for rye and sugar beet, when N fertilization was also sufficient; high K rates benefited potato yield trends. We highlight the importance of adequate nutrient supply for maintaining yield progress to actually achieve the crop genetic yield potentials. The explicit consideration of the interaction between crop fertilization and genetic progress on a long-term basis is critical for understanding past and projecting future yield trends. Long-term fertilization experiments provide a suitable data source for such studies.
Abstract
Interest in localized agri-food systems has grown significantly in recent years. They are associated with several benefits and are seen as important for rural development. An important share of the academic debate addresses the contribution of localized food systems to the current and/or future sustainability of agriculture. Sustainability is defined in several ways, but many scholars recognize that sustainability can only be achieved by a combination of socio-economic, cultural, and environmental aspects. However, the attributes and indicators used for sustainability analyses also differ. Biodiversity is, for instance, often not included in analyses of environmental sustainability even if biodiversity is of crucial importance for longer-term ecological sustainability. To contribute to the debate about the importance of localized food production for sustainability from the environmental point of view, specifically with regard to biodiversity, this is therefore discussed based on the results of several studies presented in this paper. The studies focus on Nordic low-intensity livestock systems related to species-rich semi-natural grasslands. All the studies show that low-intensive agriculture and use of semi-natural grasslands may play an important role in maintaining biodiversity on both small and large scales. They also show that milk and dairy products from free-ranging livestock in heterogeneous landscapes with semi-natural grasslands may have a unique quality associated with local grazing resources. Thus, producers can combine production of food of documented high nutritional and gastronomic value with maintenance of biodiversity, i.e., localized agri-food production based on low-intensive agriculture systems and semi-natural grasslands may be a win-win recipe for both farmers and the society.
Authors
Gernot Bodner Arta Kronberga Liga Lepse Margit Olle Ingunn M. Vågen Lasma Rabante Juan A. Fernández Georgia Ntatsi Astrit Balliu Boris RewaldAbstract
European pulse production faces a continued loss of cultivated area along with decreasing or stagnant yields. Vicia faba is a traditional legume with high genetic diversity cultivated in a wide range of European climates. Therefore V. faba is promising to identify stable and high yielding genotypes for specific target environments. The Nordic-Baltic region is challenging for legume growing due to short vegetation period and heat/drought stress in continental climates. Within the pan-European Eurolegume project a set of 18 V. faba accessions containing var. minor and major local landraces and modern cultivars of different geographical origin was evaluated in multi-environmental trials. The aim of this study was to identify ideotypes for Northern Europe and reveal key phenotypic traits conferring high yield potential and stability. Four target environmental clusters represented the range of Nordic growing conditions with yield levels from 128 gm−2 to 380 gm−2. Multivariate classification differentiated distinctive groups of var. minor and var. major accessions with few overlapping genotypes, the former having higher average yield, taller structure, more pods per node and longer flowering duration. Late sowing under long-day conditions above 55°N latitudes resulted in early flowering due to short vegetative development (650 °Cd). Extended flowering duration and tall stature were the most important traits conferring high yields. A negative trade-off between yield potential and yield stability was detected, with yield advantages of stress resistant genotypes only in a limited range of low yielding target environments (< 180 gm−2). The highest yielding accessions (Latvian var. minor landrace Bauska and var. major landrace Cēres) represented a favourable combination of yield potential and stability. High temperatures at flowering, within a range of average maximum July temperatures between 20.5–24.5 °C, were identified as most critical environmental variable depressing yield levels between 38.5 (var. major) and 56.2 (var. minor) gm−2 °C−1. It was concluded that Baltic landraces are promising ideotypes, with adapted flowering phenology and morphological structure, for increased V. faba yields in Nordic target environments.
Abstract
Unmanned aerial vehicles (UAVs) are increasingly used as tools to perform a detailed assessment of post-harvest sites. One of the potential use of UAV photogrammetric data is to obtain tree-stump information that can then be used to support more precise decisions. This study developed and tested a methodology to automatically detect, segment, classify, and measure tree-stumps. Among the potential applications for single stump data, this study assessed the possibility (1) to detect and map root- and butt-rot on the stumps using a machine learning approach, and (2) directly measure or model tree stump diameter from the UAV data. The results revealed that the tree-stumps were detected with an overall accuracy of 68–80%, and once the stump was detected, the presence of root- and butt-rot was detected with an accuracy of 82.1%. Furthermore, the root mean square error of the UAV-derived measurements or model predictions for the stump diameter was 7.5 cm and 6.4 cm, respectively, and with the former systematically under predicting the diameter by 3.3 cm. The results of this study are promising and can lead to the development of more cost-effective and comprehensive UAV post-harvest surveys.
Abstract
We present a methodology for distinguishing between three types of animal movement behavior (foraging, resting, and walking) based on high-frequency tracking data. For each animal we quantify an individual movement path. A movement path is a temporal sequence consisting of the steps through space taken by an animal. By selecting a set of appropriate movement parameters, we develop a method to assess movement behavioral states, reflected by changes in the movement parameters. The two fundamental tasks of our study are segmentation and clustering. By segmentation, we mean the partitioning of the trajectory into segments, which are homogeneous in terms of their movement parameters. By clustering, we mean grouping similar segments together according to their estimated movement parameters. The proposed method is evaluated using field observations (done by humans) of movement behavior. We found that on average, our method agreed with the observational data (ground truth) at a level of 80.75% ± 5.9% (SE).
Authors
Anders Arvesen Francesco Cherubini Gonzalo del Alamo Serrano Rasmus Astrup Michael Becidan Helmer Belbo Franziska Goile Tuva Grytli Geoffrey Guest Carine Lausselet Per Kr. Rørstad Line Rydså Morten Seljeskog Øyvind Skreiberg Veena Sajith Vezhapparambu Anders Hammer StrømmanAbstract
Climate impacts of forest bioenergy result from a multitude of warming and cooling effects and vary by location and technology. While past bioenergy studies have analysed a limited number of climatealtering pollutants and activities, no studies have jointly addressed supply chain greenhouse gas emissions, biogenic CO2 fluxes, aerosols and albedo changes at high spatial and process detail. Here, we present a national-level climate impact analysis of stationary bioenergy systems in Norway based on wood-burning stoves and wood biomass-based district heating. We find that cooling aerosols and albedo offset 60–70% of total warming, leaving a net warming of 340 or 69 kg CO2e MWh−1 for stoves or district heating, respectively. Large variations are observed over locations for albedo, and over technology alternatives for aerosols. By demonstrating both notable magnitudes and complexities of different climate warming and cooling effects of forest bioenergy in Norway, our study emphasizes the need to consider multiple forcing agents in climate impact analysis of forest bioenergy.
Authors
Nanna B. Svenningsen Stephanie J Watts-Williams Erik J. Joner Fabio Battini Aikaterini Efthymiou Carla Cruz-Paredes Ole Nybroe Iver JakobsenAbstract
Arbuscular mycorrhizal fungi (AMF) colonise roots of most plants; their extra-radical mycelium (ERM) extends into the soil and acquires nutrients for the plant. The ERM coexists with soil microbial communities and it is unresolved whether these communities stimulate or suppress the ERM activity. This work studied the prevalence of suppressed ERM activity and identified main components behind the suppression. ERM activity was determined by quantifying ERM-mediated P uptake from radioisotope-labelled unsterile soil into plants, and compared to soil physicochemical characteristics and soil microbiome composition. ERM activity varied considerably and was greatly suppressed in 4 of 21 soils. Suppression was mitigated by soil pasteurisation and had a dominating biotic component. AMF-suppressive soils had high abundances of Acidobacteria, and other bacterial taxa being putative fungal antagonists. Suppression was also associated with low soil pH, but this effect was likely indirect, as the relative abundance of, e.g., Acidobacteria decreased after liming. Suppression could not be transferred by adding small amounts of suppressive soil to conducive soil, and thus appeared to involve the common action of several taxa. The presence of AMF antagonists resembles the phenomenon of disease-suppressive soils and implies that ecosystem services of AMF will depend strongly on the specific soil microbiome.
Abstract
Changes in the local flora of mountains are often explained by climate warming, but changes in grazing regimes may also be important. The aim of this study was to evaluate whether the alpine flora on summits in the Tatra Mts, Poland and Slovakia, has changed over the last 100 years, and if the observed changes are better explained by changes in sheep grazing or climate. We resurveyed the flora of 14 mountain summits initially investigated in the years 1878–1948. We used ordination methods to quantify changes in species composition. We tested whether changes in plant species composition could be explained by cessation of grazing and climate change, and whether these factors have influenced shifts in Ellenberg’s plant ecological indicator values and Raunkiaer’s life forms. Changes in alpine flora were greater on lower elevation summits, and lower on summits less accessible for sheep. More accessible summits were associated with a decrease in mean values of plant species’ light ecological indicator values over time, and a concurrent increase in temperature and nitrogen ecological indicator values. No significant relationships were found between accessibility for sheep and changes in Raunkiaer’s life-forms. Greater accessibility for sheep (meaning high historical grazing pressure) led to greater compositional changes of mountain summits compared with summits with low accessibility. Our results suggest that cessation of sheep grazing was the main factor causing changes in the species composition of resurveyed mountain summits in the Tatra Mts, while climate change played a more minor role.
Abstract
Pathogenic soft rot Enterobacteriaceae (SRE) belonging to the genera Pectobacterium and Dickeya cause diseases in potato and numerous other crops. Seed potatoes are the most important source of infection, but how pathogen-free tubers initially become infected remains an enigma. Since the 1920s, insects have been hypothesized to contribute to SRE transmission. To validate this hypothesis and to map the insect species potentially involved in SRE dispersal, we have analyzed the occurrence of SRE in insects recovered from potato fields over a period of 2 years. Twenty-eight yellow sticky traps were set up in 10 potato fields throughout Norway to attract and trap insects. Total DNA recovered from over 2,000 randomly chosen trapped insects was tested for SRE, using a specific quantitative PCR (qPCR) TaqMan assay, and insects that tested positive were identified by DNA barcoding. Although the occurrence of SRE-carrying insects varied, they were found in all the tested fields. While Delia species were dominant among the insects that carried the largest amount of SRE, more than 80 other SRE-carrying insect species were identified, and they had different levels of abundance. Additionally, the occurrence of SRE in three laboratory-reared insect species was analyzed, and this suggested that SRE are natural members of some insect microbiomes, with herbivorous Delia floralis carrying more SRE than the cabbage moth (Plutella xylostella) and carnivorous green lacewing larvae (Chrysoperla carnea). In summary, the high proportion, variety, and ubiquity of insects that carried SRE show the need to address this source of the pathogens to reduce the initial infection of seed material.
Authors
Bernardo Duarte Irene Martins Rui Rosa Ana R. Matos Michael Roleda Thorsten B. H. Reusch Aschwin H. Engelen Ester A. Serrão Gareth A. Pearson João C. Marques Isabel Caçador Carlos M. Duarte Alexander Oliver JüterbockAbstract
Marine macrophytes are the foundation of algal forests and seagrass meadows–some of the most productive and diverse coastal marine ecosystems on the planet. These ecosystems provide nursery grounds and food for fish and invertebrates, coastline protection from erosion, carbon sequestration, and nutrient fixation. For marine macrophytes, temperature is generally the most important range limiting factor, and ocean warming is considered the most severe threat among global climate change factors. Ocean warming induced losses of dominant macrophytes along their equatorial range edges, as well as range extensions into polar regions, are predicted and already documented. While adaptive evolution based on genetic change is considered too slow to keep pace with the increasing rate of anthropogenic environmental changes, rapid adaptation may come about through a set of non-genetic mechanisms involving the functional composition of the associated microbiome, as well as epigenetic modification of the genome and its regulatory effect on gene expression and the activity of transposable elements. While research in terrestrial plants demonstrates that the integration of non-genetic mechanisms provide a more holistic picture of a species’ evolutionary potential, research in marine systems is lagging behind. Here, we aim to review the potential of marine macrophytes to acclimatize and adapt to major climate change effects via intraspecific variation at the genetic, epigenetic, and microbiome levels. All three levels create phenotypic variation that may either enhance fitness within individuals (plasticity) or be subject to selection and ultimately, adaptation. We review three of the most important phenotypic variations in a climate change context, including physiological variation, variation in propagation success, and in herbivore resistance. Integrating different levels of plasticity, and adaptability into ecological models will allow to obtain a more holistic understanding of trait variation and a realistic assessment of the future performance and distribution of marine macrophytes. Such multi-disciplinary approach that integrates various levels of intraspecific variation, and their effect on phenotypic and physiological variation, is of crucial importance for the effective management and conservation of seagrasses and macroalgae under climate change.
Authors
Zelalem Bekeko Chemeda Fininsa Dagne Wegary Temam Hussien Shimelis Hussien Belachew Asalf TadesseAbstract
Ten elite maize inbred lines were selected based on all over per se performance and gray leaf spot disease reaction. Crosses were made in a 10×10 half-diallel mating design to produce 45 F1 single cross hybrids. The experiment was conducted at Bako national maize research center in 2015 and evaluation of the crosses were made at Bako and Jimma research centers in 2016 by using alpha lattice design with three replications including three commercial checks. All the necessary yield, agronomic and GLS disease data were recorded. In all the studied traits highly significant genotypic differences were observed indicating the existence of genetic variability among the crosses. Analysis of variance for the combining ability indicated GCA and SCA mean squares were significant at (P < 0.001) for all traits except for anthesis-silking interval, ear per plant, ear diameter, lesion length and width. The ratios of GCA/SCA variances for agronomic parameters and all disease parameters were greater than unity except for that of first disease appearance implying the predominance of additive gene actions. Among all inbred lines, P1, P4, P7, P8 and P9 were identified as desirable sources of resistant genes for GLS disease resistance with positive days of first disease appearance and negative disease incidence, severity and AUDPC values for GCA effects. From the analysis of epidemiological data and disease progress curves the Logistic model (R2=96.5) better described the disease progress curves than the Gompertz model (R2=92.5) indicating the presence of delayance in epidemics and the inflection point of the GLS. P1, P7 and P8 were identified as a good general combiners for yield, yield related traits and GLS disease parameters. Thus, these parents were recommended to be used in breeding programs with a purpose of developing high yielder and GLS resistant single cross hybrids. In conclusion this study identified potential high yielding and GLS resistant single cross hybrids (CML-395/CML-383, CML-395/Sc-22, CML-395/CML-197 and CML-383/CML-197). Therefore, it is recommended that these hybrids can be used for direct production where this disease is the most prevalent and/or for further breeding programs in generating novel hybrids for future use.
Authors
Charlotte Gaillard Hamzah Shahbaz Bhatti Margarita Novoa-Garrido Vibeke Lind Michael Roleda Martin Riis WeisbjergAbstract
The potential of seaweeds as alternative protein source was investigated in relation to their amino acid (AA) profiles and the ruminal and total tract digestibility of these AAs. Three red (Mastocarpus stellatus, Palmaria palmata, and Porphyra sp.), four brown (Alaria esculenta, Laminaria digitata, Pelvetia canaliculata, and Saccharina latissima), and two green (Cladophora rupestris. and Ulva sp.) seaweed species were used in this study (hereafter, referred to by Genus name only). All seaweeds were collected in Bodø, Northern Norway, during Spring and Autumn in 2014 and 2015, except Ulva, which was only sampled in Autumn of both years, and Saccharina which was not sampled in Spring 2014. All the samples were studied for AA concentration. Six species (Cladophora, Laminaria, Mastocarpus, Palmaria, Porphyra and Ulva) were selected for the more resource demanding in situ study. Species and season interactively affected the content of total AA in crude protein in different seaweeds investigated (P=0.02), with values ranging from 67.2 for Laminaria in Spring to 90.2 gAA/16 g N for Ulva in Autumn. in situ AA degradability was also species specific. The seasonality of total AA in crude protein of different seaweed species mostly did not affect their ruminal degradability, except for alanine, while species and season interactively affected proline’s ruminal degradability. The total tract degradability showed that for Laminaria and Mastocarpus, methionine followed by leucine, isoleucine, histidine and lysine, were protected against rumen degradation. These protections seemed to be acid labile allowing digestion in the lower digestive tract. However, due to high indigestible fractions, these two seaweeds provided low amounts of AA to the intestines. Total tract AA digestibility values were the highest for Porphyra (906 g/kg) followed by Palmaria (843 g/kg) and the green seaweeds. To conclude, Laminaria and Mastocarpus are beneficial sources for bypass protein supply as they contain AA protected against rumen degradation. Based on their amount of AA and their AA degradability, Porphyra, followed by Palmaria and the green seaweeds (Ulva and Cladophora) can be considered as relevant sources of protein for ruminants.
Authors
Juraj Galko Bjørn Økland Troy Kimoto Slavomír Rell Milan Zúbrik Andrej Kunca Jozef Vakula Andrej Gubka Christo NikolovAbstract
A warmer climate may potentially have a strong effect on the health status of European oak forests by weakening oak trees and facilitating mass reproduction of wood boring insects. We did a laboratory experiment in Slovakia to study the response of major pest beetles of oak and their parasitoids to different temperature regimes as background for predicting climatic effects and improving management tools of European oak forests. With higher temperatures the most important oak pest Scolytus intricatus emerged much earlier, which indicate that completion of a second generation and increased damage further north in European oak forests may be possible. Lower temperatures gave longer larval galleries and more offspring per parents but still lower beetle production due to semivoltine life cycle. For buprestids and longhorn beetles warmer temperatures resulted in more emerging offspring and a shift towards earlier emergence in the same season, but no emergence in the first season indicated that a change to univoltine populations is not likely. Reduced development success of parasitoids at the highest temperatures (25/30 °C) indicates a loss of population regulation for pest beetle populations. A warmer climate may lead to invasion of other population-regulating parasitoids, but also new serious pest may invade. With expected temperature increases it is recommended to use trap trees both in April and in June, and trap trees should be removed within 2 months instead 1 year as described in the current standard.
Abstract
In many applications, estimates are required for small sub-populations with so few (or no) sample plots that direct estimators that do not utilize auxiliary variables (e.g. remotely sensed data) are not applicable or result in low precision. This problem is overcome in small area estimation (SAE) by linking the variable of interest to auxiliary variables using a model. Two types of models can be distinguished based on the scale on which they operate: i) Unit-level models are applied in the well-known area-based approach (ABA) and are commonly used in forest inventories supported by fine-resolution 3D remote sensing data such as airborne laser scanning (ALS) or digital aerial photogrammetry (AP); ii) Area-level models, where the response is a direct estimate based on a sample within the domain and the explanatory variables are aggregated auxiliary variables, are less frequently applied. Estimators associated with these two model types can make use of sample plots within domains if available and reduce to so-called synthetic estimators in domains where no sample plots are available. We used both model types and their associated model-based estimators in the same study area with AP data as auxiliary variables. Heteroscedasticity, i.e. for continuous dependent variables typically an increasing dispersion of re- siduals with increasing predictions, is often observed in models linking field- and remotely sensed data. This violates the model assumption that the distribution of the residual errors is constant. Complying with model assumptions is required for model-based methods to result in reliable estimates. Addressing heteroscedasticity in models had considerable impacts on standard errors. When complying with model assumptions, the precision of estimates based on unit-level models was, on average, considerably greater (29%–31% smaller standard errors) than those based on area-level models. Area-level models may nonetheless be attractive because they allow the use of sampling designs that do not easily link to remotely sensed data, such as variable radius plots.
Authors
Michael Roleda Jorunn Skjermo Hélène Marfaing Rosa Jonsdottir Celine Rebours Anna Gietl Dagmar B. Stengel Udo NitschkeAbstract
This study represents a large-scale investigation into iodine contents in three commercially important and edible seaweed species from the North Atlantic: the brown algae Saccharina latissima and Alaria esculenta, and the red alga Palmaria palmata. Variability among and within species were explored in terms of temporal and spatial variations in addition to biomass source. Mean iodine concentration in bulk seaweed biomass was speciesspecific: Saccharina > Alaria > Palmaria. Iodine contents of Saccharina biomass were similar between years and seasons, but varied significantly between sampling locations and biomass sources. In Alaria and Palmaria, none of the independent variables examined contributed significantly to the small variations observed. Our data suggest that all three species are rich sources of iodine, and only 32, 283, or 2149 mg dry weight of unprocessed dry biomass of Saccharina, Alaria, or Palmaria, respectively, meets the recommended daily intake levels for most healthy humans.
Authors
Svein Solberg Johannes May Wiley Steven Bogren Johannes Breidenbach Torfinn Torp Belachew Gizachew ZelekeAbstract
Monitoring changes in forest height, biomass and carbon stock is important for understanding the drivers of forest change, clarifying the geography and magnitude of the fluxes of the global carbon budget and for providing input data to REDD+. The objective of this study was to investigate the feasibility of covering these monitoring needs using InSAR DEM changes over time and associated estimates of forest biomass change and corresponding net CO2 emissions. A wall-to-wall map of net forest change for Uganda with its tropical forests was derived from two Digital Elevation Model (DEM) datasets, namely the SRTM acquired in 2000 and TanDEM-X acquired around 2012 based on Interferometric SAR (InSAR) and based on the height of the phase center. Errors in the form of bias, as well as parallel lines and belts having a certain height shift in the SRTM DEM were removed, and the penetration difference between X- and C-band SAR into the forest canopy was corrected. On average, we estimated X-band InSAR height to decrease by 7 cm during the period 2000–2012, corresponding to an estimated annual CO2 emission of 5 Mt for the entirety of Uganda. The uncertainty of this estimate given as a 95% confidence interval was 2.9–7.1 Mt. The presented method has a number of issues that require further research, including the particular SRTM biases and artifact errors; the penetration difference between the X- and C-band; the final height adjustment; and the validity of a linear conversion from InSAR height change to AGB change. However, the results corresponded well to other datasets on forest change and AGB stocks, concerning both their geographical variation and their aggregated values.
Abstract
In recent years, rising competition for water coupled with new environmental regulations has exerted pressure on water allocations for turfgrass irrigation. In this article, we reviewed published scientific and industry evidence on the agronomic and environmental impacts of turfgrass irrigation using a robust systematic review methodology. Our focus was on the links between (i) irrigation management (amount and frequency), (ii) agronomic responses to irrigation (turf quality, growth rates and rooting) and (iii) environmental impacts (nitrogen leaching). Based on an initial screening of 653 studies and data extracted from 83 papers, our results show that in most cases, under moderate levels of deficit irrigation (50%–60% of actual evapotranspiration), turf quality can be maintained at an acceptable level but with lower water consumption compared to irrigating back to field capacity. Irrigation beyond field capacity was found to increase the risk of nutrient leaching. However, evidence also showed that the concentration and total loss of urn:x-wiley:09312250:media:jac12265:jac12265-math-0001 in leachate were influenced more by nitrogen (N) rates, soil characteristics, turfgrass species and turfgrass growth rates than by irrigation practices. Our analyses suggest that turfgrass irrigation should be scheduled to apply water at moderate levels of deficit irrigation, sufficient to maintain turfgrass quality but limited to promote a deep and extensive rooting system. The findings provide new insights and valuable evidence for both scientists and practitioners involved in turfgrass research and management.
Authors
Klaus Mittenzwei Hugo Storm Thomas HeckeleiAbstract
Seeking the answer to the question of how farmers allocate their limited labor resources has a long tradition in the agricultural economics literature (Schultz 1990, Benjamin 1992). The role of off-farm income to close the income gap between farm households and non-farm households has been emphasized by various scholars (e.g. Schmitt 1989, Gardner 1992, Mishra et al. 2002). Ahearn et al. (2006) focus on the role of government subsidies on the allocation decision. This chapter contributes to this literature. In particular, we investigate the relationship of farmers’ decisions to combine farm income with off-farm wage income and to what extent this affects their total household income. To this end, we combine taxpayer information and agricultural data at the farm household level to study labor decisions and the income of Norwegian farm households and compare with the income situation of all households. Using data of almost 40 000 farm households for the year 2009, we find that farm households obtain an income that is on average larger than that of all Norwegian households. However, there is a large variation. Descriptive statistical analysis looking at joint distributions of key structural variables, policy support and income at farm level provides unique information.
Authors
Nick Hutchings Seyda Özkan-Gülzari Michel de Haan Daniel L. SandarsAbstract
The European Union Effort Sharing Regulation (ESR) will require a 30% reduction in greenhouse gas (GHG) emissions by 2030 compared with 2005 from the sectors not included in the European Emissions Trading Scheme, including agriculture. This will require the estimation of current and future emissions from agriculture, including dairy cattle production systems. Using a farm-scale model as part of a Tier 3 method for farm to national scales provides a more holistic and informative approach than IPCC (2006) Tier 2 but requires independent quality control. Comparing the results of using models to simulate a range of scenarios that explore an appropriate range of biophysical and management situations can support this process by providing a framework for placing model results in context. To assess the variation between models and the process of understanding differences, estimates of GHG emissions from four farm-scale models (DairyWise, FarmAC, HolosNor and SFARMMOD) were calculated for eight dairy farming scenarios within a factorial design consisting of two climates (cool/dry and warm/wet)×two soil types (sandy and clayey)×two feeding systems (grass only and grass/maize). The milk yield per cow, follower:cow ratio, manure management system, nitrogen (N) fertilisation and land area were standardised for all scenarios in order to associate the differences in the results with the model structure and function. Potential yield and application of available N in fertiliser and manure were specified separately for grass and maize. Significant differences between models were found in GHG emissions at the farm-scale and for most contributory sources, although there was no difference in the ranking of source magnitudes. The farm-scale GHG emissions, averaged over the four models, was 10.6 t carbon dioxide equivalents (CO2e)/ha per year, with a range of 1.9 t CO2e/ha per year. Even though key production characteristics were specified in the scenarios, there were still significant differences between models in the annual milk production per ha and the amounts of N fertiliser and concentrate feed imported. This was because the models differed in their description of biophysical responses and feedback mechanisms, and in the extent to which management functions were internalised. We conclude that comparing the results of different farm-scale models when applied to a range of scenarios would build confidence in their use in achieving ESR targets, justifying further investment in the development of a wider range of scenarios and software tools.
Abstract
Enabling automated 3D mapping in forests is an important component of the future development of forest technology, and has been garnering interest in the scientific community, as can be seen from the many recent publications. Accordingly, the authors of the present paper propose the use of a Simultaneous Localisation and Mapping algorithm, called graph-SLAM, to generate local maps of forests. In their study, the 3D data required for the mapping process were collected using a custom-made, mobile platform equipped with a number of sensors, including Velodyne VLP-16 LiDAR, a stereo camera, an IMU, and a GPS. The 3D map was generated solely from laser scans, first by relying on laser odometry and then by improving it with robust graph optimisation after loop closures, which is the core of the graph-SLAM algorithm. The resulting map, in the form of a 3D point cloud, was then evaluated in terms of its accuracy and precision. Specifically, the accuracy of the fitted diameter at breast height (DBH) and the relative distance between the trees were evaluated. The results show that the DBH estimates using the Pratt circle fit method could enable a mean estimation error of approximately 2 cm (7–12%) and an RMSE of 2.38 cm (9%), whereas for tree positioning accuracy, the mean error was 0.0476 m. The authors conclude that robust SLAM algorithms can support the development of forestry by providing cost-effective and acceptable quality methods for forest mapping. Moreover, such maps open up the possibility for precision localisation for forestry vehicles.
Abstract
Worldwide semi-natural habitats of high biological value are in decline. Consequently, numerous AgriEnvironment Schemes (AESs) intended to halt biodiversity loss within these habitats have been implemented. One approach has been the application of “adaptive management”, where scientific knowledge is applied alongside the traditional ecological knowledge (TEK) of stakeholders in order to establish an integrated approach that is adjusted as outcomes are assessed. In this paper we examine the effectiveness of the adaptive management approach of Norway’s Action Plan for Hay Meadows (APHM). Twenty-nine hay meadows from fourteen farms in the county of Møre og Romsdal were ecologically surveyed over a 2 year period. Interviews were also conducted with owners and land managers to explore TEK and management issues. The interdisciplinary study found that the disembedding of hay meadow management from its initial commercial purpose (in particular the loss of much of the livestock from the region) has contributed to a significant loss of TEK – which is now largely limited to knowledge of how the fields were managed recently. While, the APHM is limiting biodiversity decline by promoting traditional practices there were indications that the standardisation of management actions might negatively affect species composition in the long term. More critically, continued farm abandonment within the region means that without alternatives to management by farmers many of these meadows are likely to disappear in the next couple of decades. We conclude that adaptive management provides an effective short-term means of preserving hay meadows, but long term conservation will require a means of addressing the continued decline of local farming communities.
Abstract
The effectiveness of generating virtual transects on unmanned aerial vehicle-derived orthomosaics was evaluated in estimating the extent of soil disturbance by severity class. Combinations of 4 transect lengths (5–50 m) and five sampling intensities (1–20 transects per ha) were used in assessing traffic intensity and the severity of soil disturbance on six post-harvest, cut-to-length (CTL) clearfell sites. In total, 15% of the 33 ha studied showed some trace of vehicle traffic. Of this, 63% of was categorized as light (no visible surface disturbance). Traffic intensity varied from 787 to 1256 m ha−1, with a weighted mean of 956 m ha−1, approximately twice the geometrical minimum achievable with CTL technology under perfect conditions. An overall weighted mean of 4.7% of the total site area was compromised by severe rutting. A high sampling intensity, increasing with decreasing incidence of soil disturbance, is required if mean estimation error is to be kept below 20%. The paper presents a methodology that can be generally applied in forest management or in similar land-use evaluations.
Authors
Seyda Özkan-Gülzari Bouda Vosough Ahmadi Alistair W. StottAbstract
Impaired animal health causes both productivity and profitability losses on dairy farms, resulting in inefficient use of inputs and increase in greenhouse gas (GHG) emissions produced per unit of product (i.e. emissions intensity). Here, we used subclinical mastitis as an exemplar to benchmark alternative scenarios against an economic optimum and adjusted herd structure to estimate the GHG emissions intensity associated with varying levels of disease. Five levels of somatic cell count (SCC) classes were considered namely 50,000 (i.e. SCC50), 200,000, 400,000, 600,000 and 800,000 cells/mL (milliliter) of milk. The effects of varying levels of SCC on milk yield reduction and consequential milk price penalties were used in a dynamic programming (DP) model that maximizes the profit per cow, represented as expected net present value, by choosing optimal animal replacement rates. The GHG emissions intensities associated with different levels of SCC were then computed using a farm-scale model (HolosNor). The total culling rates of both primiparous (PP) and multiparous (MP) cows for the five levels of SCC scenarios estimated by the model varied from a minimum of 30.9% to a maximum of 43.7%. The expected profit was the highest for cows with SCC200 due to declining margin over feed, which influenced the DP model to cull and replace more animals and generate higher profit under this scenario compared to SCC50. The GHG emission intensities for the PP and MP cows with SCC50 were 1.01 kg (kilogram) and 0.95 kg carbon dioxide equivalents (CO 2 e) per kg fat and protein corrected milk (FPCM), respectively, with the lowest emissions being achieved in SCC50. Our results show that there is a potential to reduce the farm GHG emissions intensity by 3.7% if the milk production was improved through reducing the level of SCC to 50,000 cells/mL in relation to SCC level 800,000 cells/mL. It was concluded that preventing and/or controlling subclinical mastitis consequently reduces the GHG emissions per unit of product on farm that results in improved profits for the farmers through reductions in milk losses, optimum culling rate and reduced feed and other variable costs. We suggest that further studies exploring the impact of a combination of diseases on emissions intensity are warranted.
Abstract
1 The European spruce bark beetle Ips typographus is a damaging pest on spruce in Europe. Beetle interactions with tree species originating outside the natural range of the beetle are largely unknown and may be unpredictable because trees without a co-evolutionary history with the beetle may lack effective defences. 2 The terpenoid composition and breeding suitability for I. typographus of the historic host Norway spruce Picea abies were compared with two evolutionary naïve spruces of North American origin that are extensively planted in North-West Europe: Sitka spruce Picea sitchensis and Lutz spruce Picea glauca x lutzii. 3 The bark of all three species had a similar chemical composition and similar levels of total constitutive terpenoids, although Norway spruce had higher total induced terpenoid levels. 4 Beetles tunnelling in the three spruce species produced similar amounts of aggregation pheromone. Controlled breeding experiments showed that I. typographus could produce offspring in all three species, with a similar offspring length and weight across species. However, total offspring production was much lower in Sitka and Lutz spruce. 5 Overall, the results of the present study suggest that I. typographus will be able to colonize Sitka and Lutz spruce in European plantations and in native spruce forests in North America if introduced there.
Abstract
1. Large-scale pattern-oriented approaches are useful to understand the multi-level processes that shape the genetic structure of a population. Matching the scales of patterns and putative processes is both a key to success and a challenge. 2. We have developed a simple statistical approach, based on variogram analysis, that identifies multiple spatial scales where the population pattern, in this case genetic structure, have highest expression (i.e. the spatial scales at which the strength of patterning of isolation-by-distance (IBD) residual variance reached maximum) from empirical data and, thus, at which scales it should be studied relative to the underlying processes. The approach is applicable to any spatially explicit pairwise data, including genetic, morphological or ecological distance or similarity of individuals, populations and ecosystems. To exemplify possible applications of this approach, we analysed microsatellite genotypes of 1,530 brown bears from Sweden and Norway. 3. The variogram approach identified two scales at which population structure was strongest, thus indicating two different scale-dependent processes: home-rangerelated processes at scales <35 km, and subpopulation division at scales >98 km. On the basis of this, we performed a scale-explicit analysis of genetic structure using DResD analysis and compared the results with those obtained by the Bayesian clustering implemented in structure. 4. We found that the genetic cluster identified in central Scandinavia by Structure is caused by IBD, with distinct gene flow barriers to the south and north. We discuss possible applications and research perspectives to further develop the approach.
Abstract
In regions with short growth seasons, it is of great importance to use potato (Solanum tuberosum L.) seed tubers with a high growth vigour and a short growth cycle. Such qualities may be obtained by treatments advancing the physiological age of the seed tubers. In this study, we have exposed tubers from four cultivars to various combinations of temperature and light conditions (greensprouting) for 3–7 months in controlled climate. Subsequent sprout quality, seed tuber health and performance were studied in laboratory, greenhouse and field trials. Satisfactory short, sturdy and leafy sprouts were produced even after 7 months storage at 15 °C under light exposure. An assay of black scurf (Rhizoctonia solani) on the tuber skin showed that light exposure significantly reduced the occurrence compared with dark-stored tubers, while the average effect of storage temperatures was insignificant. In general, green-sprouting advanced emergence and plant growth by 1–2 weeks, and showed early tuber initiation and growth, compared to untreated material. Yields, 107 days after planting in the field trial, did not deviate significantly from untreated tubers. However, plant development at harvest was in accordance with general responses to physiological ageing of potato seed tubers, i.e. still tall and immature plants from untreated tubers, and short and mature plants from aged tubers. Results demonstrated the possibility of successful long-term storage of potato seed tubers in light at elevated temperatures and a potential for earlier harvests and higher early yields from such treatments.
Authors
Málfríður Bjarnadóttir Björn Viðar Aðalbjörnsson Anna Nilsson Rasa Slizyte Michael Roleda Guðmundur Óli Hreggviðsson Ólafur H. Friðjónsson Rósa JónsdóttirAbstract
The red seaweed Palmaria palmata has previously been reported to have high protein content high in essential amino acids. To extract the proteins a rigid cell wall consisting mainly of β-(1→4)/β-(1→3)-D-xylans must be disrupted. Different methods have been used to overcome this problem along with various methods used for protein evaluation. In this study, the effect of enzymatic pre-treatment on protein extraction was examined. Both enzymatic hydrolysis with xylanase and protease were tested. The amino acid content of the fractions was examined after extraction. The amino acid composition was similar to what has previously been reported; P. palmata was high in essential amino acids. Accordingly, a nitrogen-to-protein conversion factor was calculated for each fraction individually and protein results were compared with calculation using the proximate 6.25 conversion factor. The nitrogen-to-protein conversion factor varied between fractions but all factors were significantly lower than the popularly used 6.25 indicating that this conversion factor for processed P. palmata is effectively and considerably overestimating the protein content. Enzymatic pre-treatment with xylanase resulted in enhanced amino acid content and successful protein extraction. Enzymatic hydrolysis using protease resulted in higher protein content in the liquid extract compared to hydrolysis with xylanase, due to the release of proteins, peptides, and amino acids. Therefore, hydrolysis with protease is not suitable to extract proteins from P. palmata with the method described within this study but might be an optimal method to examine the bioactivity by extracting the protein hydrolysates. However, the result from this study confirm that hydrolysis with xylanase is a feasible choice to extract proteins of good quality from P. palmata.
Abstract
Background: Generalized height-diameter curves based on a re-parameterized version of the Korf function for Norway spruce (Picea abies (L.) Karst.), Scots pine (Pinus sylvestris L.) and silver birch (Betula pendula Roth) in Norway are presented. The Norwegian National Forest Inventory (NFI) is used as data base for estimating the model parameters. The derived models are developed to enable spatially explicit and site sensitive tree height imputation in forest inventories as well as future tree height predictions in growth and yield scenario simulations. Methods: Generalized additive mixed models (gamm) are employed to detect and quantify potentially non-linear effects of predictor variables. In doing so the quadratic mean diameter serves as longitudinal covariate since stand age, as measured in the NFI, shows only a weak correlation with a stands developmental status in Norwegian forests. Additionally the models can be locally calibrated by predicting random effects if measured height-diameter pairs are available. Based on the model selection of non-constraint models, shape constraint additive models (scam) were fit to incorporate expert knowledge and intrinsic relationships by enforcing certain effect patterns like monotonicity. Results: Model comparisons demonstrate that the shape constraints lead to only marginal differences in statistical characteristics but ensure reasonable model predictions. Under constant constraints the developed models predict increasing tree heights with decreasing altitude, increasing soil depth and increasing competition pressure of a tree. A two-dimensional spatially structured effect of UTM-coordinates accounts for the potential effects of large scale spatially correlated covariates, which were not at our disposal. The main result of modelling the spatially structured effect is lower tree height prediction for coastal sites and with increasing latitude. The quadratic mean diameter affects both the level and the slope of the height-diameter curve and both effects are positive. Conclusions: In this investigation it is assumed that model effects in additive modelling of height-diameter curves which are unfeasible and too wiggly from an expert point of view are a result of quantitatively or qualitatively limited data bases. However, this problem can be regarded not to be specific to our investigation but more general since growth and yield data that are balanced over the whole data range with respect to all combinations of predictor variables are exceptional cases. Hence, scam may provide methodological improvements in several applications by combining the flexibility of additive models with expert knowledge.
Authors
Anne-Grete Roer Hjelkrem Heidi Udnes Aamot Guro Brodal Einar Strand Torfinn Torp Simon G. Edwards Ruth Dill-Macky Ingerd Skow HofgaardAbstract
High concentrations of the mycotoxins HT-2 and T-2 (HT2 + T2), primarily produced by Fusarium langsethiae, have occasionally been detected in Norwegian oat grains. In this study, we identified weather variables influencing accumulation of HT2 + T2 in Norwegian oat grains. Oat grain samples from farmers’ fields were collected together with weather data (2004–2013). Spearman rank correlation coefficients were calculated between the HT2 + T2 contamination in oats at harvest and a range of weather summarisations within estimated phenological windows of growth stages in oats (tillering, flowering etc.). Furthermore, we developed a mathematical model to predict the risk of HT2 + T2 in oat grains. Our data show that adequate predictions of the risk of HT2 + T2 in oat grains at harvest can be achieved, based upon weather data observed during the growing season. Humid and cool conditions, in addition to moderate temperatures during booting, were associated with increased HT2 + T2 accumulation in harvested oat grains, whereas warm and humid weather during stem elongation and inflorescence emergence, or cool weather and absence of rain during booting reduced the risk of HT2 + T2 accumulation. Warm and humid weather immediately after flowering increased the risk, while moderate to warm temperatures and absence of rain during dough development, reduced the risk of HT2 + T2 accumulation in oat grains. Our data indicated that HT2 + T2 contamination in oats is influenced by weather conditions both pre- and post-flowering. These findings are in contrast with a previous study examining the risk of deoxynivalenol contamination in oat reporting that toxin accumulation was mostly influenced by weather conditions from flowering onwards.
Authors
Chunjing Qiu Dan Zhu Philippe Ciais Bertrand Guenet Gerhard Krinner Shushi Peng Mika Aurela Christian Bernhofer Christian Brümmer Syndonia Bret-Harte Housen Chu Jiquan Chen Ankur R. Desai Jiří Dušek Eugénie S. Euskirchen Krzysztof Fortuniak Lawrence B. Flanagan Thomas Friborg Mateusz Grygoruk Sébastien Gogo Thomas Grünwald Birger U. Hansen David Holl Elyn Humphreys Miriam Hurkuck Gerard Kiely Janina Klatt Lars Kutzbach Chloé Largeron Fatima Laggoun-Défarge Magnus Lund Peter M. Lafleur Xuefei Li Ivan Mammarella Lutz Merbold Mats B. Nilsson Janusz Olejnik Mikaell Ottosson-Löfvenius Walter C. Oechel Frans-Jan W. Parmentier Matthias Peichl Norbert Pirk Olli Peltola Włodzimierz Pawlak Daniel Rasse Janne Rinne Gaius R. Shaver Hans Peter Schmid Matteo Sottocornola Rainer Steinbrecher Torsten Sachs Marek Urbaniak Donatella Zona Klaudia ZiemblinskaAbstract
Peatlands store substantial amounts of carbon and are vulnerable to climate change. We present a modified version of the Organising Carbon and Hydrology In Dynamic Ecosystems (ORCHIDEE) land surface model for simulating the hydrology, surface energy, and CO2 fluxes of peatlands on daily to annual timescales. The model includes a separate soil tile in each 0.5° grid cell, defined from a global peatland map and identified with peat-specific soil hydraulic properties. Runoff from non-peat vegetation within a grid cell containing a fraction of peat is routed to this peat soil tile, which maintains shallow water tables. The water table position separates oxic from anoxic decomposition. The model was evaluated against eddy-covariance (EC) observations from 30 northern peatland sites, with the maximum rate of carboxylation (Vcmax) being optimized at each site. Regarding short-term day-to-day variations, the model performance was good for gross primary production (GPP) (r2 = 0.76; Nash–Sutcliffe modeling efficiency, MEF = 0.76) and ecosystem respiration (ER, r2 = 0.78, MEF = 0.75), with lesser accuracy for latent heat fluxes (LE, r2 = 0.42, MEF = 0.14) and and net ecosystem CO2 exchange (NEE, r2 = 0.38, MEF = 0.26). Seasonal variations in GPP, ER, NEE, and energy fluxes on monthly scales showed moderate to high r2 values (0.57–0.86). For spatial across-site gradients of annual mean GPP, ER, NEE, and LE, r2 values of 0.93, 0.89, 0.27, and 0.71 were achieved, respectively. Water table (WT) variation was not well predicted (r2 < 0.1), likely due to the uncertain water input to the peat from surrounding areas. However, the poor performance of WT simulation did not greatly affect predictions of ER and NEE. We found a significant relationship between optimized Vcmax and latitude (temperature), which better reflects the spatial gradients of annual NEE than using an average Vcmax value.
Abstract
A series of semi-continuous incubation experiments were conducted with the coccolithophore Emiliania huxleyi strain NIWA1108 (Southern Ocean isolate) to examine the effects of five environmental drivers (nitrate and phosphate concentrations, irradiance, temperature, and partial pressure of CO2 (pCO2// on both the physiological rates and elemental composition of the coccolithophore. Here, we report the alteration of the elemental composition of E. huxleyi in response to the changes in these environmental drivers. A series of dose–response curves for the cellular elemental composition of E. huxleyi were fitted for each of the five drivers across an environmentally representative gradient. The importance of each driver in regulating the elemental composition of E. huxleyi was ranked using a semiquantitative approach. The percentage variations in elemental composition arising from the change in each driver between present-day and model-projected conditions for the year 2100 were calculated. Temperature was the most important driver controlling both cellular particulate organic and inorganic carbon content, whereas nutrient concentrations were the most important regulator of cellular particulate nitrogen and phosphorus of E. huxleyi. In contrast, elevated pCO2 had the greatest influence on cellular particulate inorganic carbon to organic carbon ratio, resulting in a decrease in the ratio. Our results indicate that the different environmental drivers play specific roles in regulating the elemental composition of E. huxleyi with wide-reaching implications for coccolithophore-related marine biogeochemical cycles, as a consequence of the regulation of E. huxleyi physiological processes.
Authors
Björn Ringselle Erik Bertholtz Ewa Magnuski Lars Olav Brandsæter Kjell Mangerud Göran BergkvistAbstract
Tillage controls perennial weeds, such as Elymus repens, partly because it fragments their underground storage organs. However, tillage is difficult to combine with a growing crop, which limits its application. The aim of this study was to evaluate how soil vertical cutting with minimum soil disturbance and mowing affect the growth and competitive ability of E. repens in a grass–clover crop. A tractor-drawn prototype with vertical disks was used to fragment E. repens rhizomes with minimal soil and crop disturbance. In experiments performed in 2014 and 2015 at a field site close to Uppsala, Sweden, the rhizomes were fragmented before crop sowing (ERF), during crop growth (LRF), or both (ERFCLRF). Fragmentation was combined with repeated mowing (yes/no) and four companion crop treatments (none, Italian ryegrass, white clover, and grass/clover mixture). The results showed that in the grass–clover crop, rhizome fragmentation reduced E. repens rhizome biomass production and increased Italian ryegrass shoot biomass. ERF and LRF both reduced E. repens rhizome biomass by about 38% compared with the control, while ERFCLRF reduced it by 63%. Italian ryegrass shoot biomass was increased by 78% by ERF, 170% by LRF and 200% by ERFCLRF. Repeated mowing throughout the experiment reduced E. repens rhizome biomass by about 75%. Combining repeated mowing with rhizome fragmentation did not significantly increase the control effect compared to mowing alone. We concluded that rhizome fragmentation using vertical disks can be used both before sowing and during crop growth to enhance the controlling effect of grass–clover crops on E. repens.