Geir Kjølberg Knudsen
Senior Engineer
(+47) 456 62 262
geir.knudsen@nibio.no
Place
Landvik
Visiting address
Reddalsveien 215, 4886 Grimstad
Abstract
Background The order Lepidoptera has an abundance of species, including both agriculturally beneficial and detrimental insects. Molecular data has been used to investigate the phylogenetic relationships of major subdivisions in Lepidoptera, which has enhanced our understanding of the evolutionary relationships at the family and superfamily levels. However, the phylogenetic placement of many superfamilies and/or families in this order is still unknown. In this study, we determine the systematic status of the family Argyresthiidae within Lepidoptera and explore its phylogenetic affinities and implications for the evolution of the order. We describe the first mitochondrial (mt) genome from a member of Argyresthiidae, the apple fruit moth Argyresthia conjugella. The insect is an important pest on apples in Fennoscandia, as it switches hosts when the main host fails to produce crops. Results The mt genome of A. conjugella contains 16,044 bp and encodes all 37 genes commonly found in insect mt genomes, including 13 protein-coding genes (PCGs), two ribosomal RNAs, 22 transfer RNAs, and a large control region (1101 bp). The nucleotide composition was extremely AT-rich (82%). All detected PCGs (13) began with an ATN codon and terminated with a TAA stop codon, except the start codon in cox1 is ATT. All 22 tRNAs had cloverleaf secondary structures, except trnS1, where one of the dihydrouridine (DHU) arms is missing, reflecting potential differences in gene expression. When compared to the mt genomes of 507 other Lepidoptera representing 18 superfamilies and 42 families, phylogenomic analyses found that A. conjugella had the closest relationship with the Plutellidae family (Yponomeutoidea-super family). We also detected a sister relationship between Yponomeutoidea and the superfamily Tineidae. Conclusions Our results underline the potential importance of mt genomes in comparative genomic analyses of Lepidoptera species and provide valuable evolutionary insight across the tree of Lepidoptera species.
Authors
Lars T. Havstad John Ingar Øverland Trygve S. Aamlid Trond Gunnarstorp Geir Kjølberg Knudsen Jon SælandAbstract
Desiccation with diquat about one week before seed harvest has been common practise in Norwegian clover seed production. However, after withdrawal of diquat in 2020, clover seed growers no longer have desiccators available. In 2019 and 2020, six field trials in red clover and two field trials in white clover were carried out to evaluate alternative chemical products at different rates and at two different spraying dates, either early at 50% mature seed heads and / or late at 65% mature seed heads. Products included, either for one or two years, was Spotlight Plus (carfentrazonethyl), Beloukha (pelargonic acid), Glypper (glyphosate), Gozai (Pyraflufen-ethyl), Harmonix LeafActive (acetic acid), Harmonix FoliaPlus (pelargonic acid), Flurostar (fluroxypyr) and Saltex (sodium chloride) and liquid urea-based fertilizers. In addition, swathing was examined as an alternative in two red clover trials in 2020. While none of the tested chemicals were superior to diquat, the most promising alternatives were Harmonix FoliaPlus and Harmonix LeafActive in red clover or Harmonix FoliaPlus in white clover. Although usually less effective than these products, Beloukha also had an acceptable desiccation effect, especially when sprayed early and late. Swathing before harvest, using finger bar cutters, was an effective drying method under favourable weather conditions.
Authors
Hans Martin Hanslin Ellen Johanne Svalheim Harald Bratli J. Wissmann Geir Kjølberg Knudsen J. Kollmann Trygve S. AamlidAbstract
No abstract has been registered