Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
1999
Authors
Roger Roseth Harald Bjørnstad Per Kraft Bill WarnerAbstract
No abstract has been registered
Authors
Roger Roseth Harald BjørnstadAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
C. Walter L.J. Grace S.S. Donaldson J. Moody J.E. Gemmell S. van der Maas Harald Kvaalen Anders LönneborgAbstract
An efficient Biolistic transformation technology was developed to stably transform Picea abies (L.) Karst. Several embryogenic tissue lines were tested for proliferation on standard embryogenesis media. Transient transformation studies with these lines were performed to optimize the parameters for genetic transformation. Selection conditions for transgenic tissue based on the nptII resistance gene in combination with the antibiotic geneticin were defined such that only transgenic P. abies lines were able to develop. Nontransgenic tissue was completely inhibited under these conditions. Stable integration of a uidA reporter gene and a nptII resistance gene into the genome of P. abies was achieved and more than 200 mature embryos were regenerated for every transformation event. Histochemical and fluorometric analysis indicated strong expression of the uidA gene in transgenic material. ELISA studies to detect and quantify the nptII gene product as well as polymerase chain reaction and Southern blotting confirmed the presence and integration of uidA and nptII genes into the P. abies genome. Transgenic P. abies plants from nine independent transformation events were recovered and are currently growing in a greenhouse for genetically modified organisms, awaiting field release.
Authors
Ketil KohmannAbstract
No abstract has been registered
Authors
Ann Merete Furuberg GjedtjernetAbstract
No abstract has been registered
Authors
Bal Ram Singh Åsgeir Rossebø Almås Carl Einar Amundsen Hanne Margrete Meltzer Jan AlexanderAbstract
No abstract has been registered
Abstract
Potential response of forest soils to sulphur deposition in the Norwegian-Russian border area in the surroundings of the Pechenganikel smelters, the major sulphur emitters in the northern Europe, has been assessed with the PROFILE model. The release rate of base cations due to weathering range from 0.05 to 0.28 kmol(c)/ha/yr in the 0 - 50 cm soil layer, thus demonstrating the high sensitivity of the coarse and thin podzols studied. Calculated steady-state BC/Al values are significantly lower than the presumed critical value of 1, which indicate possible negative effect on vegetation through soil acidification. According to the model calculations future sulphur deposition have to be very low in order to stop the ongoing acidification and prevent vegetation damage. However, model assumptions, uncertainty in input data and critical chemical values applied implies that modelling results must be interpreted carefully
Abstract
A field experiment was carried out to test the hypothesis that treatment of Norway spruce trees with the Ips typographus-transmitted blue-stain fungus Ceratocystis polonica enhances tree resistance to later mass attack by this bark beetle. Twenty-five mature trees were pre-treated by inoculating a non-lethal dose of the fungus into the bark, while 18 trees served as untreated controls. Three and a half weeks after treatment a bark beetle attack was initiated by attaching dispensers with I. typographus pheromone to the tree trunks. A significantly larger proportion (67%) of the control trees than of the pre-treated trees (36%) were killed by the beetle attack. The result is discussed in relation to recent results regarding defence mechanisms in Norway spruce trees.
Abstract
No abstract has been registered