Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2009
Abstract
In trees adapted to cold climates, conditions during autumn and winter may influence the subsequent timing of bud burst and hence tree survival during early spring frosts. We tested the effects of two temperatures during, dormancy induction Mid mild spells (MS) during chilling, on the timing of bud burst in three Picea abies (L.) Karst. provenances (58-66 degrees N). One-year-old seedlings were induced to become dormant at temperatures of 12 or 21 degrees C applied during 9 weeks of short days (12-h photoperiod). The seedlings were then moved to cold storage and given either continuous chilling at 0.7 degrees C (control), or chilling interrupted by one 14-day MS it either 8 or 12 degrees C. Interruptions with MS were staggered throughout the 175-day chilling period, resulting in 10 MS differing in date of onset. Subsets of seedlings were moved to forcing conditions (12-h photoperiod, 12 degrees C) throughout the chilling period, to assess dormancy status different timings of the MS treatment. Finally, after 175 days of chilling, timing of bud burst was assessed in a 24-h photoperiod at 12 degrees C (control and MS-treated seedlings). The MS treatment did not significantly affect days to bud burst when given early (after 7-35 chilling days). When MS was given after 49 chilling days or later, the seedlings burst bud earlier than the controls, and the difference increased with increasing length of the chilling period given before the MS. The 12 degrees C MS treatment was more effective than the 8 degrees C MS treatment, and the difference remained constant after the seedlings had received 66 or more chilling days before the MS treatment was applied. In all provenances, a constant temperature of 21 degrees C during dormancy induction resulted in more dormant seedlings (delayed bud burst) than a constant temperature of 12 degrees C, but this did not delay the response to the MS treatment.
Abstract
No abstract has been registered
Abstract
The estrogenic mycotoxin zearalenone (ZON) produced by some Fusarium spp. causes reproductive problems and hyperestrogenic syndromes in mammals. In an effort to elucidate the molecular pathways of ZON production, we present a comparative real-time quantitative polymerase chain reaction expression study of seven contiguous genes in the ZON biosynthetic cluster on sterile rice and during wheat and oat infection. Under ZON production on rice, the polyketide synthase (PKS) genes PKS4 and PKS13, alcohol oxidase FG12056 gene, and transcriptional regulator FG02398 gene showed similarly upregulated patterns, whereas the nonribosomal peptide synthetase (NPS) FG02394, the K+ channel beta subunit FG12015, and the protein kinase FG02399 displayed a variant pattern. During the same time period under wheat infection when no ZON was produced, the PKS genes and the NPS were downregulated relative to rice whereas the K+ channel beta subunit gene FG12015 was markedly upregulated, suggesting that it may play a role in the infection process. This is the first expression study of ZON biosynthetic genes in planta. The results give insight into the regulation and activities of the ZON gene cluster under different experimental systems and suggest a connection between ZON and a K+ channel that could reveal a novel function for ZON in Fusarium spp.
Authors
Arve Heistad Razak Seidu Andreas Svarstad Flø Adam Paruch Jon Fredrik Hanssen Thor-Axel StenströmAbstract
Tie long-term use of a filter-based, on-site wastewater treatment system increases nutrient discharge to receiving waters and may reduce its hygienic barrier efficiency. The main purpose of this research was to assess the hygienic barrier efficiency and the associated health risks of an on-site system that had exceeded its 5-yr design capacity with respect to phosphorus (P) removal. The system was investigated for bacteria and virus removal and assessed with respect to potential health risks in relation to reuse of effluent for irrigation. The system consists of a septic tank, a pressure-dosed vertical flow biofilter, and an up-flow filter unit with lightweight clay aggregates. The total P concentration in the effluent had increased gradually from initially <0.1 mg P L-1 during the first 2 yr of operation to 1.8 mg P L-1 after 5.3 yr. Escherichia coli was used as an indicator organism for fecal bacteria removal, whereas bacteriophages phi X174 and Salmonella typhimurium phage 28B (S.t. 28B) were used to model enteric virus removal. An overall decrease in E. coli removal occurred from a complete (approximately 5.6 log(10)) reduction during the first 3 yr of operation to 2.6 log(10) reduction. The removal amounts of the bacteriophages phi X174 and S.t. 28B were 3.9 and 3.7 log(10), respectively. Based on removal of S.t. 28B, the risks of rotavirus infection and disease for the investigated scenarios were above the acceptable level of 10(-4) and 10(-3), respectively, as defined by the World Health Organization.
Authors
Knut Anders Hovstad Silje Borvik Mikael OhlsonAbstract
In modern agricultural landscapes, large herbivores are often restricted in their mobility or are found at low densities, and other animal vectors may therefore be important for seed dispersal. In our study, a range of plant species were able to disperse by attaching seeds to, and having their seeds retained in, the fox fur some distance. We suggest that the red fox may be an important vector for epizoochorous seed dispersal in the agricultural landscape.
Authors
Hege Nordheim-Viken Harald Volden Marit JørgensenAbstract
The aim of this experiment was to investigate effects of temperature and photoperiod on growth characteristics, chemical composition and indigestible fibre (IaNDF) in timothy harvested at three defined maturity stages. Two cultivars of timothy, Engmo (69 degrees N) and Grindstad (59 degrees N) were grown in a controlled environment using a phytotron, and were exposed to two different temperature regimes (21/15 degrees C and 15/9 degrees C, 12h/12h)and photoperiods (18 and 24h). Plants were harvested at beginning of heading (BH), full heading (FH) and anthesis (AN). Leaves and stems were separated prior to chemical analyses and ruminal in situ incubation for 288 h was used to determine indigestible aNDF (IaNDF) content. Plants grown at low temperature used almost twice the number of days to reach AN, compared to the highest temperature regime. This explained the lower leaf:stem ratio (LSR), higher aNDF content and lower crude protein (CP) content in low temperature plants. The effect of temperature was often dependent on maturity stage. However, the content of IaNDF and lignin(sa) was only affected by maturity stage at harvest. The content of IaNDF and lignin increased by 70% and 35% respectively from BH to AN. The lack of response in IaNDF and lignin(sa) content and dry matter digestibility to temperature was probably caused by a combination of direct and indirect temperature effects. Photoperiod affected growth characteristics, but had minor effects on chemical composition and IaNDF. Due to the lower LSR, Engmo had higher IaNDF and lignin(sa) content than Grindstad. This study showed a high positive correlation (0.93) between IaNDF and lignin(sa). Interactions between temperature and maturity stage affect the growth and nutritive characteristics of timothy. However, only maturity stage affected IaNDF and lignin(sa) content. (C) 2009 Elsevier B.V. All rights reserved.
Abstract
No abstract has been registered
Authors
Knut Anders Hovstad Mikael OhlsonAbstract
Plant litter is an important determinant of seed germination and seedling establishment. Positive effects of litter have received considerable attention, but few studies have explicitly tested whether seedlings are more facilitated by conspecific litter compared to heterospecific litter. In order to contrast conspecific and heterospecific facilitative effects on seedling establishment, we used Anthriscus sylvestris, Angelica sylvestris, Pimpinella saxifraga and different combinations of their seeds and litter seedbeds as a model system. Although litter had a significant species-specific effect on seedling emergence, we found no evidence of strictly conspecific facilitation. Anthriscus sylvestris displayed a positive response to all types of litter. In contrast, there was a clear negative effect of conspecific litter in Pimpinella saxifraga. Activated carbon did not modify the negative effect, indicating that chemical compounds were not the cause. Our study suggests a high level of idiosyncrasy in response to litter at the species level.
Authors
Ole Martin Bollandsås Ingvild Rekstad Erik Næsset Ingvald RøsbergAbstract
The objectives of this study were (1) to develop models for estimation of total above-ground biomass, tree crown biomass and stem biomass of mountain birch (Betula pubescens spp. czerepanovii), and (2) to test the stability of the relationships between biomass and biophysical tree properties across geographical regions and tree size ranges. The models were developed using a mixed modelling approach accounting for the hierarchical structure of the data that originated from sample plots. Diameter at breast height, tree height, and the ratio between height and diameter were candidate explanatory variables, but only diameter was statistically significant (p0.05). The model fit values (pseudo-R 2) were 0.91, 0.60 and 0.85 for the three respective models. A substantial part of the model random errors could be attributed to between-plot variations. The conclusion related to objective (1) was that the models are well suited for biomass prediction of mountain birch in the mountain areas of southern Norway. Furthermore, models reported in previous research that had been calibrated on data from other regions were applied on the current data set. The results indicate that models calibrated for small trees produced predictions diverging from the observed values of the current data set. The differences between predicted and observed values also seem to vary along a site productivity gradient. Still, even though the differences between predicted and observed values using the different models varied quite a lot, the relationships were relatively stable within certain limits. The conclusion related to objective (2) was that biomass models can be applied outside the region for which they were developed, which in many cases is necessary because local models do not exist. However, the properties of the model development data related to tree size range and site productivity should be similar to those of the area for which predictions are being made.
Abstract
Genetic parameters were estimated for wood density and spiral grain in two long-term field trials with families of Picea abies (L.) Karst., and for microfibril angle (MfA) and model-predicted wood stiffness (MOEest) at one of the sites. The trials were located at 600-720 m altitude in Norway and the progenies, which were a sample of 13 half-sib families from plus-trees in a breeding population, were 33 years old from seed when measured. Significant genetic variation (p0.05) was found for all wood quality traits. The narrow-sense heritability was estimated to be 0.50 for density (across two sites), 0.38 for MfA, 0.29 for MOEest and 0.37 for spiral grain (across two sites). No significant genotype by environment interactions were found for density or spiral grain (p0.05). Genetic relationships between ring width and wood quality traits were negative for density and MOEest, and positive for MfA. Site index and competition had major effects on wood density and predicted MOEest but not on MfA and spiral grain.