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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

1995

Abstract

Roots of Picea abies seedlings were inoculated with pathogenic Pythium dimorphum. The ongoing lignification process, the accumulation of lignin, and the distribution of flavanols and condensed tannins (CT), were related to the concurrent visual disease symptoms, hyphal colonization, and cellular changes. The hyphae ramified in both the cortex and the stele within 24 h. Three days after inoculation the concentration of lignin had increased to a level twice that in noninfected tissues. With histochemical staining, the accumulation of lignin and the ongoing lignification were co-located in the inner cortex layer 4-6 days after infection. Comparison of temporal onset of lignification with the timing of the intruding hyphae shows that this defense response occurs too late to effectively prevent the pathogen from spreading. Flavanols and CT were also detected in both infected and noninfected roots.Increased staining of these compounds in infected roots was often detected in the inner cortex. Electron microscopic studies revealed that at least some of these phenolic compounds were localized either as spherules or as a layer appressed to the tonoplast of the central vacuole.

Abstract

An alkaline sample solution in combination with the complexing agent diethylenetriaminepentaacetic acid (DTPA) was employed to minimize interference from Ni and Co in the determination of As and Te by hydride generation inductively coupled plasma atomic emission spectrometry. Tetrahydroborate was also added to the sample solution, before the hydride was generated by acidification vith HCl in a continuous-flow system. The masking method was used for the determination of As and Te in the reference material NIST SRM 899 Nickel Ahoy. For the determination of As in a copper sample, the interference from Cu was removed by precipitation of the hydroxide and filtration. The precipitation method was used for the determination of As in the reference material NIST SRM 398 Unalloyed Copper V; hydride generation atomic absorption spectrometry was employed to determine the concentration of As.