Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2024
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Dagnija Lazdina Valeriu Caisin Bertold Heinze Dana Dina Kolevska Kjersti Holt Hanssen (+17 forfattere) et al.Abstract
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Xiaoyu Yuan Keya Xu Fang Yan Zhiyuan Liu Carl Jonas Jorge Spetz Huanbin Zhou Xiaojie Wang Huaibing Jin Xifeng Wang Yan LiuAbstract
Wheat dwarf virus (WDV, genus Mastrevirus, family Geminiviridae) is one of the causal agents of wheat viral disease, which severely impacts wheat production in most wheat-growing regions in the world. Currently, there is little information about natural resistance against WDV in common wheat germplasms. CRISPR/Cas9 technology is being utilized to manufacture transgenic plants resistant to different diseases. In the present study, we used the CRISPR/Cas9 system targeting overlapping regions of coat protein (CP) and movement protein (MP) (referred to as CP/MP) or large intergenic region (LIR) in the wheat variety ‘Fielder’ to develop resistance against WDV. WDV-inoculated T1 progenies expressing Cas9 and sgRNA for CP/MP and LIR showed complete resistance against WDV and no accumulation of viral DNA compared with control plants. Mutation analysis revealed that the CP/MP and LIR targeting sites have small indels in the corresponding Cas9-positive plants. Additionally, virus inhibition and indel mutations occurred in T2 homozygous lines. Together, our work gives efficient results of the engineering of CRISPR/Cas9-mediated WDV resistance in common wheat plants, and the specific sgRNAs identified in this study can be extended to utilize the CRISPR/Cas9 system to confer resistance to WDV in other cereal crops such as barley, oats, and rye.
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Authors
Monica Sanden Johanna Eva Bodin Nur Duale Kristian Prydz Volha Shapaval Tage Thorstensen Anne-Marthe Ganes Jevnaker Ville Erling SipinenAbstract
The Norwegian Scientific Committee for Food and Environment (VKM) has assessed an application for approval of the genetically modified maize MON 94804 for food and feed uses, import and processing in the EU. In accordance with an assignment specified by the Norwegian Food Safety Authority (NFSA) and the Norwegian Environment Agency (NEA), VKM assesses whether genetically modified organisms (GMOs) intended for the European market can pose risks to human or animal health, or the environment in Norway. VKM assesses the scientific documentation regarding GMO applications seeking approval for use of GMOs as food and feed, processing, or cultivation. The EU Regulation 1829/2003/EC (Regulation) covers living GMOs that fall under the Norwegian Gene Technology Act, as well as processed food and feed from GMOs (dead material) that fall under the Norwegian Food Act. The regulation is currently not part of the EEA agreement or implemented in Norwegian law. Norway conducts its own assessments of GMO applications in preparation for the possible implementation of the Regulation. In accordance with the assignment by NFSA and NEA, VKM assesses GMO applications during scientific hearings initiated by the European Food Safety Authority (EFSA), as well as after EFSA has published its own risk assessment of a GMO, up until EU member countries vote for or against approval in the EU Commission. The assignment is divided into three stages. (link) Maize MON 94804 MON 94804 is a genetically modified maize developed via Agrobacterium tumefaciens -mediated transformation of maize cells. Maize MON 94804 expresses a micro-RNA (miRNA) transcript, that leads to reduced levels of the hormone gibberellin in the plant. Gibberellin is involved in, i.a., plant stem elongation. Thus, maize MON 94804 plants grow shorter than other maize. The scientific documentation provided in the application for maize MON 94804 is adequate for risk assessment, and in accordance with EFSA guidance on risk assessment of genetically modified plants for use in food or feed. The VKM GMO panel does not consider the introduced modifications in maize MON 94804 to imply potential specific health or environmental risks in Norway, compared to EU-countries. The EFSA scientific Opinion is adequate also for Norwegian conditions. Therefore, a full risk assessment of maize MON 94804 was not performed by the VKM GMO Panel.
Authors
Ville Erling Sipinen Monica Sanden Johanna Eva Bodin Nur Duale Anne-Marthe Ganes Jevnaker Kristian Prydz Volha Shapaval Tage ThorstensenAbstract
The Norwegian Committee for Food and Environment (VKM) has performed a preliminary assessment of an application for authorization for the genetically modified maize event DP202216 in the EAA. The scope of the application includes all uses of maize DP202216 and sub-combinations independently of their origin equivalent to the uses of any other maize grain and forage. The assessment was performed in connection with EFSAs (European Food Safety Authorities) public hearing of application EFSA-GMO-NL-2019-159, on request from the Norwegian Food Safety Authority and the Norwegian Environment Agency. The assessment of maize DP202216 is based on information provided by the applicant in the application EFSA-GMO-NL-2019-159, and relevant peer-reviewed scientific literature. Maize DP202216 has the potential to enhanced grain yield, and provides tolerance to glufosinate-ammonium herbicides. Authorisation process for genetically modified organisms Through the EEA Agreement, the EU Directive 2001/18/EC on deliberate release into the environment of genetically modified organisms is implemented in Norwegian law. Norway is therefore affiliated with the GMO authorisation process in the EU. In the EU, both GMOs and derived products are regulated by the Directive and Regulation 1829/2003/EC. The Regulation concerns genetically modified food and feed and is currently not a part of the EEA Agreement. In preparation for a legal implementation of the Regulation in Norwegian law, Norway follows the EU proceedings for GMO applications. When a company seeks approval of a genetically modified organism, the application is submitted to the national competent authority of an EU Member State, which forwards the application to EFSA. EFSA then submits the application for a public hearing across all EEA countries. VKM conducts its own review of the application and sends its comments to EFSA. EFSA then completes their scientific opinion based on information from the applicant, comments from EEA member countries and independent literature. The scientific opinion is then issued to the European Commission. VKM submitted their comments on application EFSA-GMO-NL-2019-159 to EFSA before the deadline January 3, 2020.
Authors
Monica Sanden Johanna Eva Bodin Nur Duale Kristian Prydz Anne-Marthe Ganes Jevnaker Volha Shapaval Ville Erling Sipinen Tage ThorstensenAbstract
The Norwegian Committee for Food and Environment (VKM) has performed a preliminary assessment of an application for authorization for the genetically modified maize event DP202216 in the EAA. The scope of the application includes all uses of maize DP202216 and sub-combinations independently of their origin equivalent to the uses of any other maize grain and forage. The assessment was performed in connection with EFSAs (European Food Safety Authorities) public hearing of application EFSA-GMO-NL-2019-159, on request from the Norwegian Food Safety Authority and the Norwegian Environment Agency. The assessment of maize DP202216 is based on information provided by the applicant in the application EFSA-GMO-NL-2019-159, and relevant peer-reviewed scientific literature. Maize DP202216 has the potential to enhanced grain yield, and provides tolerance to glufosinate-ammonium herbicides. Authorisation process for genetically modified organisms Through the EEA Agreement, the EU Directive 2001/18/EC on deliberate release into the environment of genetically modified organisms is implemented in Norwegian law. Norway is therefore affiliated with the GMO authorisation process in the EU. In the EU, both GMOs and derived products are regulated by the Directive and Regulation 1829/2003/EC. The Regulation concerns genetically modified food and feed and is currently not a part of the EEA Agreement. In preparation for a legal implementation of the Regulation in Norwegian law, Norway follows the EU proceedings for GMO applications. When a company seeks approval of a genetically modified organism, the application is submitted to the national competent authority of an EU Member State, which forwards the application to EFSA. EFSA then submits the application for a public hearing across all EEA countries. VKM conducts its own review of the application and sends its comments to EFSA. EFSA then completes their scientific opinion based on information from the applicant, comments from EEA member countries and independent literature. The scientific opinion is then issued to the European Commission. VKM submitted their comments on application EFSA-GMO-NL-2019-159 to EFSA before the deadline January 3, 2020.