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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2010

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Abstract

Norwegian agriculture is mainly dominated by grass-based milk and livestock production, so winter damage to overwintering grasses may have large economic consequences. We assessed the impact of climate change on the winter survival of timothy (Phleum pratense L) and perennial ryegrass (Lolium perenne L) under Norwegian conditions using agroclimatic indices and a simulation model of frost tolerance. This study was based on locally adjusted future climate scenarios (two for the period 2071-2100; one for the period 2020-2049) for six important agricultural regions, represented by one location each. We proposed and validated a rough way to estimate the daily minimum air temperatures from scenario data. compared with the control period 1961-1990, the future hardening period will be shortened by up to 21 days. As a consequence, the modelled maximum frost tolerance is expected to be reduced by up to 3.9 degrees C and 1.9 degrees C for timothy and perennial ryegrass, respectively, under the warmest scenario. In spite of this reduction, the plants are expected to be hardy enough to withstand the predicted autumn frosts, and we also expect a general reduction in the risk of winter frost injuries. The plant data available to this study suggest that agroclimatic indices developed for Canadian conditions can be useful for assessing the hardening status in timothy and perennial ryegrass. However, such indices are less suitable for assessing the risk of plant injury related to frost and ice encasement in Norway, since they do not account for the dynamics of cold adaptation. Although less snow is expected, in most cases this will not be accompanied by an increase in the risk of ice encasement injuries. However, a slight increase in the number of ice encasement events was predicted for one location. An earlier start of growth was predicted for all locations, accompanied at one coastal location by a slightly increased predicted risk of spring frosts. There is little risk of winter injuries related to frost and ice encasement in the hardier grass species timothy. The better overwintering conditions in general indicate that it will be possible to grow perennial ryegrass in areas where it is not grown today, provided the risk of fungal diseases does not increase. (C) 2010 Elsevier B.V. All rights reserved.

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Abstract

Timothy (Phleum pratense L) is the most important forage grass in Scandinavia and it is therefore highly interesting to study how it will perform in a changing climate. In order to model winter survival, the dynamics of hardening and dehardening must be simulated with satisfactory precision. We investigated an early timothy frost tolerance model (LT50 model), and an LT50 model for winter wheat. Based on the assumption that timothy has no vernalization requirement, unlike winter wheat, but does have the ability to adapt to cold temperatures in a process linked to stage of development, two alternative versions of the winter wheat model were also constructed. In total, these four candidate models were calibrated by a Bayesian approach for the timothy cultivar Engmo. The candidate models were validated using independent observations on LT50 in timothy at different locations reflecting differences in climate. A sensitivity analysis, using the Morris method, to identify important model parameters suggested that there is a connection between frost tolerance and stage of plant development, even if there is no vernalization requirement. The simplified winter wheat model was selected as the best candidate model for LT50 in timothy based on model selection criteria and its ability to capture the hardening and dehardening processes. The results from the Bayesian calibration suggest that there are no major regional differences in Norway calling for regional calibration. However, cultivar-specific calibration is probably required, since there are hardy and less hardy cultivars within the same species. A functional LT50 model would allow risk assessments to be made of future winter survival using specifically tailored and downscaled climate scenarios. (C) 2010 Elsevier B.V. All rights reserved.

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Abstract

Tomato plants (Solanum lycopersicum, cv. Suzanne) were subjected to complete nutrient solution or a solution without nitrogen (N), and placed at different temperatures and light conditions to test the effects of environment on flavonoids and caffeoyl derivatives and related gene expression. N depletion during 4-8 days resulted in enhanced levels of flavonoids and caffeoyl derivatives. Anthocyanins showed pronounced increased levels when lowering the growth temperature from 24 degrees C to 18 degrees C or 12 degrees C. Flavonol levels increased when the light intensity was increased from 100 mu mol m(-2) s(-1) to 200 mu mol m(-2) s(-1) PAR. Synergistic effects of the various environmental factors were observed. The increase in content of quercetin derivatives in response to low temperatures was only found under conditions of N depletion, and especially at the higher light intensity. Expression of structural genes in the phenylpropanoid and flavonoid pathways, PAL (phenylalanine ammonia lyase), CHS (chalcone synthase), F3H (flavanone 3-hydroxylase), and FLS (flavonol synthase) increased in response to N depletion, in agreement with a corresponding increase in flavonoid and caffeoyl content. Expression of these structural genes generally also increased in response to lower temperatures. As indicated through expression studies and correlation analysis, effects of N depletion were apparently mediated through the overall regulators of the pathway the MYB transcription factor ANT1 (ANTHOCYANIN 1) and SlJAF13 (a bHLH transcription factor orthologue of petunia JAF13 and maize RED genes). A PAL gene (PAL6) was identified, and correlation analysis was compatible with PAL6 being an actively expressed gene with function in flavonoid synthesis. (C) 2009 Elsevier Ltd. All rights reserved.

Abstract

The fruit quality of European blueberry (EB) is mainly determined by taste compounds (sugars, acids, flavour) and health-beneficial structures generally denoted as antioxidants (vitamin C, phenolic acids, flavonols, anthocyanins, proanthocyanidins). Content and compound composition is strongly affected by the growth environment regarding light, temperature, water and edaphic factors. In order to assess genotypic relationships (northern and southern clones of EB) and environmental impact (temperature, day length) on berry quality parameters, a high-throughput system for blueberry metabolite profiling of nutritional compounds was established based on gas chromatography coupled with mass spectrometry (GC/MS). Dried methanol/H2O extracts from fresh-frozen berry tissue were derivatized, and subjected to GC/MS in order to detect polar compounds such as organic acids from Krebs-cycle, amino acids, sugars, polyols, and partly secondary metabolites (phenols, flavonoids). In addition, general quality parameters such as total phenols, total anthocyanins and antioxidant capacity (FRAP) were measured. Fructose (5 g), glucose (5 g), and sucrose (0.5 g/ 100 g f.w. at average) were the most abundant carbohydrates, together with high levels of organic acids such as citric acid (1.3 g), quinic acid (1.6 g), and malic acid (0.3 g/ 100 g f.w. at average). More than 50 metabolites per sample (identified compounds and not-annotated mass spectral tags) could be detected, and established the basis for multivariate statistics using principal component analysis, hierarchical clustering, and metabolite network analysis. Genotypic differences, modulation of metabolite pools and biosynthetic relationships are being discussed in-depth.

Abstract

Two female and two male cultivars have previously been released as a result of clone evaluation at Bioforsk Nord Holt. Selection criteria have been number of pistils or stamens per flower, number of flowers and number of shoots per m2. Currently a new group of clones are evaluated with the aim of finding new cultivars for release. The clones are collected from different parts of Norway, as well as from England and Spitsbergen. Preliminary results from harvesting 2005, 2006, 2007 and 2008 indicate good production potential for a couple of the tested clones. In addition to prior selection criteria based on berry yield, the levels of total anthocyanins and total phenols have been analyzed. This includes studies on the role of female clone, male pollinator and temperature on berry quality.

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Abstract

In this study we investigated the interaction between temperature and genotype on fruit development and levels of total phenols and anthocyanins in cloudberry. The experiment was done in a phytotron using one female (‘Fjellgull") and one hermaphroditic (‘Nyby") cultivar. Plants were grown at 9, 12, 15 and 18°C in 24-h photoperiod. The female cultivars were pollinated with pollen from a male (‘Apollen") clone and from the hermaphrodite clone. Parthenocarpic fruit development was induced by gibberellic acid (GA3). Ripe berries were frozen individually at -80°C and stored until analyses. There was a linear, double logarithmic relationship between temperature and number of days from pollination/GA3-treatment to ripening. ‘Fjellgull" had significantly larger berries than ‘Nyby", and the largest berries were obtained at 12 and 9°C. Pollen clone did not have a significant effect on berry size. GA3 induced parthenogenesis in ‘Fjellgull" and partial parthenogenesis in ‘Nyby". In ‘Fjellgull", the parthenocarpic berries were comparable to pollinated ones at low temperatures, but at 18°C their development was restricted. The level of total anthocyanins was significantly higher in ‘Fjellgull" than in ‘Nyby", and these levels were significantly enhanced at 9 and 12°C compared to higher temperatures. Levels of total phenolic compounds were not significantly affected. In conclusion, the present results indicate that low temperature is favourable both for size and quality of cloudberries.