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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2021

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Sammendrag

For nokre av driftsystema vi granska i SusCatt-prosjektet undersøkte vi nærmare korleis ein overgang til meir grovfôrbasert fôring kan ha å seie for lønnsemda. Fem SusCatt-system blei vurdert, inkludert arbeidet i Sverige der en samanlikna effekt av rase og fôrstyrke på storfekjøttproduksjon av både oksar og kastratar frå mjølkekyr, og i Storbritannia med rein grovfôrbasert storfekjøttproduksjon frå ammekyr. I mjølkeproduksjonen, blei effekt av kraftfôrnivå på lønnsemd studert ved bruk av husdyrkontrolldata i Noreg og for italienske gardar effekten av å redusere mengde maissurfôr i rasjonen...

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Sammendrag

The widespread apicomplexan parasite Cryptosporidium parvum is responsible for severe gastrointestinal disease in humans and animals. The treatment options are limited, and the efficacy of available drugs is low. Bark contains condensed tannins (CT), which are bioactive compounds previously shown to inhibit parasite development. Here, we examined the anti-cryptosporidial properties of bark extract of Scots pine (Pinus sylvestris) against C. parvum by means of an in vitro growth inhibition test. We hypothesized that bark extracts would have dose-dependent inhibitory effects on the development of C. parvum in cell culture. Bark extracts from Scots pine extracted with acetone, methanol, and water as solvents, were investigated using human colorectal adenocarcinoma cells infected with C. parvum. Oocysts were inoculated onto the cell monolayer and bark extract was added at 7 different concentrations. Parasite growth inhibition was quantified by qPCR. The acetone and methanol extracts demonstrated a sigmoid dose-dependent inhibition of C. parvum. The IC50 values were 244.6 and 279.1 µg dry matter extract/mL, and 25.4 and 24.1 µg CT/mL, for acetone and methanol extracts, respectively. The IC50 for both extracts were similar, both with regards to the dry matter concentration of each extract and to CT concentrations. Given the limited treatment options available for Cryptosporidium spp., the evidence generated in our study encourages further investigation into the in vitro and in vivo effects of pine bark extracts against C. parvum.