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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2022

Abstract

Modern apple growing requires relatively often orchard replacement due to release of superior cultivars or introduction of new growing technologies. Most of intensive apples orchards are established in the same site where apple trees were cultivated for a long period. Continuous cultivation of the same crop causes stress to plants and often leads to abnormal plant development and decreased productivity what is known as apple replant disease (ARD). Due to ban of chemical soil disinfection, other strategies how to overcome ARD must be developed. Rootstock is becoming to be one of the most important factors to solve this problem and one of the targets of new rootstock breeding programs is rootstock resistance or tolerance to ARD. Different origin and genetic background of rootstocks led to suggest that their adaptiveness to replanted soil will be different. EUFRIN (European fruit research institutes network) Apple and pear cultivar and rootstock testing group established replant trials in several European countries where new apple rootstocks from USA, Great Britain, Poland and Russia are tested. Current paper presents results of the trial performed at the Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry in 2017-2021. On average of all rootstocks apple trees planted in the fresh soil were by 38% more vigorous and gave 71% higher yield. After the evaluation of tree growth and productivity characters rootstock G.41 was the most tolerant to ARD. Trees on rootstocks G.935, Cepiland-Pajam®2 and AR 295-6 had significantly lower yields in replant soil, while tree growth was most stunted on B.10 and AR 295-6.

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Abstract

Berries of the genus Vaccinium are highly valued health-beneficial superfoods, which are commonly subjected to adulteration and mixed with each other, or with other common berry species. A quantitative DNA-based method utilizing a chip-based digital polymerase chain reaction (dPCR) technique was developed for identifying and quantifying wild lingonberry (V. vitis-idaea) and cultivated American cranberry (V. macrocarpon). The dPCR method with species-specific primers for mini-barcoding was designed based on the indel regions found in the trnI-CAU–trnL-CAA locus in the chloroplast genome. The designed primers were able to amplify only target species, enabling to distinguish the two closely related species with good sensitivity. Our results illustrated the ability of the method to identify lingonberry and American cranberry DNA using PCR without the need for probes or further sequencing. The dPCR method could also quantify the DNA copy number in mixed samples. Based on this study, the method provides a basis for a simple, fast, and sensitive quantitative authentication analysis of lingonberry and American cranberry by dPCR. Moreover, it can also provide a platform for authentication analyses of other plant species as well by utilizing the indel regions of chloroplast genomes.

Abstract

Light exposure of potatoes induces formation of both chlorophyll (greening) and of toxic glycoalkaloids (GAs). Greening leads to rejection by consumers and thus to food waste and economic loss. The aim of this study was to (1) study light sensitivity with respect to colour changes and GA development for different Norwegian grown potato cultivars stored at 20 °C and (2) evaluate the light protective effect of selected packaging materials on colour development in cv. Folva at 6 °C and 20 °C. Potatoes of seven cultivars were stored under LED illumination for 4 days at 20 °C. Changes in colour were measured during storage by Minolta Chroma meter and by visually assessing the limit for unacceptable change of colour. The tested cultivars became unacceptable at different times (24–60 h) and differed both in absolute colour values and relative changes of values. The levels of total glycoalkaloids in cultivars with and without light exposure did not correspond well to the changes in colour. Potatoes of cultivar Folva were packaged in materials with different light barrier properties, followed by LED illumination for 4 days at 20 °C and 18 days at 6 °C. None of the tested packaging types provided sufficient protection from light. All potatoes at 20 °C were unacceptably green after 2 days in light (16 h/day) while the potatoes at 6 °C were unacceptably green after 9 days. Packaging material for potatoes cv. Folva should aim for a total light transmittance below 0.02 W/m2 to avoid development of green colour during light exposure at 20 °C in grocery stores.