Hopp til hovedinnholdet

Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2014

2013

Abstract

Three methods for extracting DNA were tested on otoliths, scales, fins, and gill tissue from European whitefish [Coregonus lavaratus (L.)]. The aim was to find time-efficient and affordable ways to simultaneously extract DNA suitable for conservation genetic studies from a large number of samples and different tissues. A rapid low-cost method led to 97 % success of microsatellite amplification in otoliths and 100 % in scales. High amplification success was achieved with fin (97 %) and gill (99 %) tissue using a salt lysis-based protocol. A commercial extraction kit delivered good results with all tissues. The findings are useful for conservation genetic studies using both contemporary and archived samples.

Abstract

Atmospheric deposition to forests has been monitored in the frame of the ICP Forests programme with sampling and analyses of bulk and throughfall deposition at several hundred forested Level II plots for more than 15 years now. Current deposition of inorganic nitrogen and sulphate is highest in Northern Central Europe as well as in some regions in southern parts. In this study we compared linear regression and MannKendall trend analyses techniques. The choice of method had an influence on the number of trends identified as being significant. We showed that the minimal detectable trends can be estimated with the mean short term temporal variability of the deposition, which is to a large extent due to meteorological variations, such as the precipitation and circulation patterns. The overall decreasing trends for inorganic N and SO42- in the past decade of about 3% and 6% require time series of about 10 and 6 years respectively to detect a trend on a plot with statistical significance. Past reduction of human emission reduced atmospheric deposition of acidifying and eutrophying compounds. This could be confirmed due to the availability of long-term data series. However, further reductions are required to reduce deposition to forests below critical loads for the whole of Europe.