Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2014
Authors
Zhibo Hamborg Sissel Haugslien Jihong Liu Clarke Carl Jonas Jorge Spetz Dag-Ragnar Blystad Qing Wang YeonKyeong Lee Astrid H Sivertsen Gry SkjesethAbstract
Chrysanthemum stunt viroid (CSVd) was first reported in US in the 1940s and is widespread in the world wherever chrysanthemum is grown. Cryotherapy of shoot tips, a new biotechnology developed in the recent years, is a novel application of plant cryopreservation techniques that allows pathogen eradication at a high frequency. Existing studies have proven that this technique can efficiently eradicate pathogens such as virus, phytoplasma and bacterium. However, up to now, there has been no report on viroid eradication. In the present study, we attempted to establish a droplet vitrification cryotherapy method for Argyranthemum and to apply it to eradicate CSVd. Results obtained so far demonstrated that cryotherapy of shoot tips alone failed to eradicate CSVd from the infected shoot tips of Argyranthemum maderense ‘Yellow Empire’. Using in situ hybridization of CSVd and histological analysis, we found that CSVd can invade meristematic cells and at the same time, these cells were able to survive following cryotherapy. These findings explained why cryotherapy of shoot tips alone could not be efficient enough to eradicate CSVd from the diseased materials. Further studies combining cold treatment with cryotherapy are under investigation for CSVd eradication.
Authors
Syed Waqas Hassan Mohammad Tahir Waheed Martin Müller Jihong Liu Clarke Zabta Khan Shimwari Andreas G. LösslAbstract
No abstract has been registered
Authors
Alice Budai Liang Wang Morten Grønli Line Tau Strand Michael Jerry Antal, Jr Samuel Abiven Alba Dieguez-Alonso Andrés Anca-Couce Daniel RasseAbstract
No abstract has been registered
Authors
Jihong Liu ClarkeAbstract
No abstract has been registered
Authors
Jihong Liu ClarkeAbstract
No abstract has been registered
Authors
Per Gundersen Shimon Ozeri Lars Vesterdal Teresa Gómez de la Bárcena Andis Lazdins Bjarni D. Sigurdsson Tryggve Persson Per Bengtsson Cecilia Akselsson Nicholas ClarkeAbstract
No abstract has been registered
Authors
Karen Refsgaard John Marshall Bryden Biancha Cavicchi Nicholas Clarke Stig Strandli Gezelius Valborg KvakkestadAbstract
No abstract has been registered
Authors
Ashraful Islam Jihong Liu Clarke Dag-Ragnar Blystad Hans Ragnar Gislerød Sissel Torre Jorunn Elisabeth OlsenAbstract
No abstract has been registered
Abstract
No abstract has been registered
Abstract
No abstract has been registered