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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2002

2001

Abstract

To find interesting gene products and to study gene expression in the green parts of Norway spruce we have sequenced 1101 cDNA clones based on mRNA isolated from needles. Here we present some preliminary findings describing the frequency of different Expressed Sequence Tags (ESTs), the ratio of similarity to known and unknown sequences present in GenBank and sequences not previously reported from Norway spruce. In addition we have tried to place the ESTs into major categories based on putative function

Abstract

Adaptation to cold and freezing temperatures is crucial for survival in temperate and boreal areas. Compared with angiosperms, little is known about the mechanisms of freezing tolerance in gymnosperms. Coniferous species such as Norway spruce (Picea abies L. Karst.) have a wide distribution in boreal areas, indicating a strong ability to adapt to lasting periods of cold and freezing temperatures. Freezing-tolerant perennial plants survive subzero temperatures by forming ice in intercellular spaces and the xylem. Certain proteins associated with pathogen attack have been found to exhibit antifreeze activity, i.e. the ability to bind and modify the growth of ice. In order to study the possible role of pathogenesis-related proteins in development of freezing tolerance in Norway spruce, we looked at the accumulation of chitinases during hardening under both artificial and natural conditions. Our findings show that chitinases and other pathogenesis-related proteins, as well as antifreeze activity, are present in needles of Norway spruce, and that they increase in response to shorter daylengths and cold temperature. It is possible that pathogenesis-related proteins accumulated in conifers during cold acclimation could also exhibit antifreeze activity, and thus play an important role during development of freezing tolerance in perennial plants such as the gymnosperm Norway spruce.