Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
1999
Authors
C. Walter L.J. Grace S.S. Donaldson J. Moody J.E. Gemmell S. van der Maas Harald Kvaalen Anders LönneborgAbstract
An efficient Biolistic transformation technology was developed to stably transform Picea abies (L.) Karst. Several embryogenic tissue lines were tested for proliferation on standard embryogenesis media. Transient transformation studies with these lines were performed to optimize the parameters for genetic transformation. Selection conditions for transgenic tissue based on the nptII resistance gene in combination with the antibiotic geneticin were defined such that only transgenic P. abies lines were able to develop. Nontransgenic tissue was completely inhibited under these conditions. Stable integration of a uidA reporter gene and a nptII resistance gene into the genome of P. abies was achieved and more than 200 mature embryos were regenerated for every transformation event. Histochemical and fluorometric analysis indicated strong expression of the uidA gene in transgenic material. ELISA studies to detect and quantify the nptII gene product as well as polymerase chain reaction and Southern blotting confirmed the presence and integration of uidA and nptII genes into the P. abies genome. Transgenic P. abies plants from nine independent transformation events were recovered and are currently growing in a greenhouse for genetically modified organisms, awaiting field release.
Authors
M.J. Latkowska Harald Kvaalen M. AppelgrenAbstract
No abstract has been registered
Authors
Harald Kvaalen M. AppelgrenAbstract
We studied how light from different light sources influences germination and postgerninative growth of plants from somatic embryos and seeds of Norway spruce (Picea abies [L.] Karst).Somatic embryos of three spruce genotypes and seeds were subjected to light from commercially available light sources: Philips TLD Blue 18W/18 (BL), Osram Fluora (FL), Philips Cool White TL 50W/33 (CW), Osram Warm White 18W/30 (WW), Philips Yellow 36W/16 (YE) and Philips TLD Red 36W/15 (RE), 18 h a day, with a photon flux (PAR) at 30 mu mol m(-2) s(-1). After 6 weeks the germination frequencies of the somatic embryo-derived plantlets were 50% under BL and 98% under RE. The corresponding mean root lengths were 6.7 and 15.4 mm. In somatic embryo-derived plantlets cultured under BL, FL, CW and WW, both roots and hypocotyls turned brown, presumably due to production of phenolic substances. Browning was less severe in somatic embryo-derived plantlets cultured under RE and YE. Under RE, the epicotyl elongated in 37% of the plantlets after 6 weeks, compared with 70% under the other light sources. Seed germination and postgerminative seedling growth was modestly influenced by light from these light sources. RE and WW initially delayed germination as compared with BL, FL and CW, but after 2 weeks, more than 90% of the seeds had germinated under all light sources. In conclusion, germination and postgerminative growth of somatic embryos of spruce is sensitive to differences in light quality, whereas seed germination and seedling growth is not.
Abstract
No abstract has been registered
1998
Authors
Dag FjeldAbstract
No abstract has been registered
Authors
M.J. Latkowska Harald Kvaalen M. AppelgrenAbstract
No abstract has been registered
Authors
M.J. Latkowska Harald Kvaalen M. AppelgrenAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Dag FjeldAbstract
No abstract has been registered
1997
Authors
Dag Fjeld K.B. HagenAbstract
No abstract has been registered