Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2012
Abstract
No abstract has been registered
Abstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Juliana PerminowAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Igor A. Yakovlev YeonKyeong Lee Bjørn Rotter Tore Skrøppa Øystein Johnsen Jorunn Elisabeth Olsen Carl Gunnar FossdalAbstract
Epigenetic memory marks establishment in Norway spruce occur exclusively during embryogenesis in response to environmental impact, and the epitype is fixated by the time the embryo is fully developed without a change in the DNA sequence. We started large scale studies aimed on identifying and characterizing of genes and regulatory elements involved in the initiation, maintenance, and heritability of epigenetic memory using candidate genes and next generation sequencing approaches. Molecular mechanisms of formation of epigenetic memory were studied on the same full-sibs family zygotic embryo in vitro cultures developed in cold (18°C) and warm (30°C) environmental conditions from proliferation till mature embryo stages. Initially we had found large set (64) of Arabidopsis epigenetic regulator gene homologs in spruce. In general, known epigenetic related genes are very well represented among spruce ESTs. Analysis of the transcription patterns of these genes using RT-PCR in epigenetically different embryogenic samples reveal specific transcription patterns on different stages of embryogenic development dependent on epitype. We are expecting to determine certain stages during embryogenesis when epigenetic memory marks are forming. At the same time, nearly no differences in transcription levels of studied genes had been found in seedlings (4 month old), originated from full-sib families clearly differed in epigenetic response. Using MACE (massive cDNA 3-end sequencing) deep mRNA sequencing on the Illumina GSII platform, we analyzed P. abies transcriptomes by comparison warm and cold originated “embryonic epitypes” developed in cold and warm environmental conditions. Significant differences in transcriptomes between epitypes revealed by high-throughput sequencing will be discussed.
Abstract
The liverwort Trichocolea tomentella is known from 65 localities in southern Norway. Almost half of these are in Hordaland county in Western Norway. T. tomentella has been observed at many localities (42%) in the last decade. On the other hand it has not been observed at 23% of the previously known localities after 1950, although a complete re-survey has not been performed. The species seems to be thriving in its main distribution centre in Western Norway, but in southeastern Norway urbanisation and modern forestry are major threats. In this part of Norway the species has probably gone extinct or is on its way to extinction at several of the localities. To preserve the species logging and draining close to springs, streams and gullies should be ceased. This will also preserve habitats that are species rich, in particular in bryophytes.
Authors
David Bell David G. Long Alan D. Forrest Michelle L. Hollingsworth Hans Blom Peter M. HollingsworthAbstract
DNA barcoding of a group of European liverwort species from the genus Herbertus was undertaken using three plastid (matK, rbcL and trnH-psbA) and one nuclear (ITS) marker. The DNA barcode data were effective in discriminating among the sampled species of Herbertus and contributed towards the detection of a previously overlooked European Herbertus species, described here as H. norenus sp. nov. This species shows clear-cut differences in DNA sequence for multiple barcode regions and is also morphologically distinct. The DNA barcode data were also useful in clarifying taxonomic relationships of the European species with some species from Asia and North America. In terms of the discriminatory power of the different barcode markers, ITS was the most informative region, followed closely by matK. All species were distinguishable by ITS alone, rbcL + matK and various other multimarker combinations.
Abstract
Springs are characterized by consistent thermal and hydrologic conditions, which enable use of spring-inhabiting organisms as sensitive indicators of biogeochemical changes in their catchments. We hypothesized that bryophytes would show a stronger response than vascular plants to changes in spring water quality because submerged bryophytes do not take up compounds from the soil. We analyzed species responses to interannual changes in spring water quality (discharge, water temperature, electrical conductivity, and pH) in 57 forest springs over 4 consecutive years. We calculated interannual turnover in species composition for bryophytes and vascular plants with the Bray–Curtis dissimilarity index. We applied regression analysis to test interannual changes in species composition of the taxonomic groups over time, and we used 2-sided t-tests to compare year-to-year changes in species composition between bryophytes and vascular plants. We used boosted regression tree (BRT) models to quantify the relative importance of different physicochemical variables and Pearson linear correlation to quantify short-term changes in vegetation relative to changes in spring-water pH. For both groups, interannual changes in species composition were significantly positively related to time scale. Bryophytes did not show a significantly stronger response than vascular plants to interannual changes in the environment. Alterations in pH and conductivity explained most of the observed interannual changes in species composition of both groups, whereas changes in water temperature and discharge were less important. However, responses of single species to environmental change may be delayed, resulting in inertia at the community and ecosystem scales. Hence, longer time periods need to be considered for a better understanding of response times of the vegetation of European forest springs to changes in spring water quality.
Abstract
No abstract has been registered