Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2019
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Pete Smith Jean-Francois Soussana Denis Angers Louis Schipper Claire Chenu Daniel Rasse Niels H. Batjes Fenny van Egmond Stephen McNeill Matthias Kuhnert Cristina Arias‐Navarro Jørgen E. Olesen Ngonidzashe Chirinda Dario Fornara Eva Wollenberg Jorge Álvaro-Fuentes Alberto Sanz-Cobena Katja KlumppSammendrag
No abstract has been registered
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No abstract has been registered
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Arne Verstraeten Elena Gottardini Nicolas Bruffaerts Bruno De Vos Elena Vanguelova Fabiana Cristofolini Sue Benham Pasi Rautio Liisa Ukonmaanaho Päivi Merilä Annika Saarto Peter Waldner Marijke Hendrickx Gerrit Genouw Peter Roskams Nathalie Cools Johan Neirynck Anita Nussbaumer Mathias Neumann Nicholas Clarke Volkmar Timmermann Karin Hansen Hans-Peter Dietrich Manuel Nicolas Maria Schmitt Anne Thimonier Katrin Meusburger Silvio Schüler Anna KowalskaSammendrag
No abstract has been registered
Forfattere
Arne Verstraeten Elena Gottardini Nicolas Bruffaerts Bruno De Vos Elena Vanguelova Fabiana Cristofolini Gerrit Genouw Anita Nussbaumer Mathias Neumann Sue Benham Pasi Rautio Liisa Ukonmaanaho Päivi Merilä Annika Saarto Jukka Reiniharju Peter Waldner Marijke Hendrickx Peter Roskams Nathalie Cools Johan Neirynck Arthur De Haeck Yvan De Bodt Geert Sioen Nicholas Clarke Volkmar Timmermann Karin Hansen Hans-Peter Dietrich Manuel Nicolas Maria Schmitt Anne Thimonier Katrin Meusburger Silvio Schüler Anna Kowalska Idalia Kasprzyk Katarzyna Borycka Łukasz Grewling Joanna Święta-Musznicka Małgorzata Latałowa Marcelina Zimny Małgorzata Malkiewicz Lars Vesterdal Iben Margrete Thomsen Miklós Manninger Donat Magyar Gergely Mányoki Hugues TiteuxSammendrag
No abstract has been registered
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Andre van Eerde Aniko Varnai John-Kristian Jameson Lisa Paruch Anders Moen Jan Haug Anonsen Piotr Chylenski Hege Særvold Steen Inger Heldal Ralph Bock Vincent Eijsink Jihong Liu ClarkeSammendrag
Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass. Plants offer a safe and cost‐effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels. Production of intact and functional protein is a prerequisite for large‐scale protein production, and extensive host‐specific post‐translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes. Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme. TrCel7A was produced in Nicotiana benthamiana using a vacuum‐based transient expression technology, and this recombinant enzyme (TrCel7Arec) was compared with the native fungal enzyme (TrCel7Anat) in terms of PTMs and catalytic activity on commercial and industrial substrates. We show that the N‐terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O‐mannosylation in the plant host as compared with the native protein. In general, the purified full‐length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate‐binding properties, which can be attributed to larger N‐glycans and lack of O‐glycans in TrCel7Arec. All in all, our study reveals that the glycosylation machinery of N. benthamiana needs tailoring to optimize the production of efficient cellulases.
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Jihong Liu ClarkeSammendrag
No abstract has been registered
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Eva BrodSammendrag
No abstract has been registered
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Marit Almvik Marianne Stenrød Randi Bolli Alice Budai Ingvill Hauso Olaug Bach Steinar Haugse Ole Martin EkloSammendrag
No abstract has been registered
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Marit Almvik Marianne Stenrød Randi Bolli Alice Budai Ingvill Hauso Olaug Bach Steinar Haugse Ole Martin EkloSammendrag
OBJECTIVES • Gain a better understanding of the fate of pesticides in the environment by also screening and detecting their metabolites • Predict and detect pesticide metabolites in soils using high resolution accurate mass (HRAM) tools; Thermo Q Exactive orbitrap and Compound DiscovererTM software. HIGHLIGHTS • We present in silico metabolism simulation to predict fungicide metabolites in soil • We present a screening method for 800 pesticides and metabolites in soil and food, exemplified with soil samples from strawberry field degradation studies (including fluopyram, boscalid and pyraclostrobin and others) • We address the lack of molecular formulas for known metabolites in current databases as an obstacle in establishing HRAM screening methods
Sammendrag
No abstract has been registered