Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2018
Authors
Signe Kynding Borgen Gry Alfredsen Johannes Breidenbach Lise Dalsgaard Gunnhild Søgaard Aaron SmithAbstract
No abstract has been registered
Abstract
Acetylation of wood can provide protection against wood deteriorating fungi, but the exact degradation me- chanism remains unclear. The aim of this study was to determine the effect of acetylation of Pinus radiata wood (weight percent gain 13, 17 and 21%) on the expression of genes involved in decay by brown-rot fungus Rhodonia placenta. Gene expression analysis using qRT-PCR captured incipient to advanced decay stages. As expected the initiation of decay was delayed as a result the degree of acetylation. However, once decay was established, the rate of degradation in acetylated samples was similar to that of unmodi fied wood. This suggests a delay in decay rather than an absolute protection threshold at higher acetylation levels. In accordance with previous studies, the oxidative system of R. placenta was more active in wood with higher degrees of acetylation and expression of cellulose active enzymes was delayed for acetylated samples compared to untreated samples. The reason for the delay in the latter might be because of the slower diffusion rate in acetylated wood or that partially acetylated cellobiose may be less effective in triggering production of saccharification enzymes. Enzymes involved in hemicellulose and pectin degradation have previously not been focused on in studies of degradation of acetylated wood. Surprisingly, CE16 carbohydrate esterase, assumed to be involved in deace- tylation of carbohydrates, was expressed significantly more in untreated samples compared to highly acetylated samples. We hypothesise that this enzyme might be regulated through a negative feedback system, where acetic acid supresses the expression. The up-regulation of two expansin genes in acetylated samples suggests that their function, to loosen the cell wall, is needed more in acetylated wood due the physical bulking of the cell wall. In this study, we demonstrate that acetylation affects the expression of specific target genes not previously re- ported, resulting in delayed initiation of decay. Thus, targeting these degradation mechanisms can contribute to improving wood protection systems.
2017
Authors
B. Lesar Miha Humar Christian Brischke Linda Meyer-Veltrup Dennis Jones N. Thaler JM Abascal Gry Alfredsen B. Brunnhuber Eva Grodås M. Irle J. Kers M. Klamer K-C Mahnert Eckhard Melcher S. Palanti M Noël E. Suttie N. Pfabigan M. TouzaAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Graham Alan Ormondroyd Gry Alfredsen Raghavalu Thirumalai Durai Prabhakaran Simon F. Curling Bronia K. Stefanowski Morwenna J. Spear Lone RossAbstract
Microbiological degradation of wood by decay fungi can cause a rapid change in the structural properties of timber which can result in both strength and mass loss. Traditional techniques for the evaluation of decay (e.g. mass loss) lack the sensitivity to evaluate the effects of the very first stages of the decay process. This paper describes the effects of initial brown rot decay, defined by the amount of Poria placenta genomic DNA (gDNA) present in the samples, on the dynamic mechanical properties of the timber. It was found that there is a correlation between the mean storage modulus of the timber and the amount of P. placenta gDNA present, and therefore the level of decay. This shows that using dynamic mechanical analysis is a viable technique that can be used to study initial decay processes.
Abstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Anna Sandak Jakub Sandak Athanasios Dimitriou Ingunn Burud Thomas Kringlebotn Thiis Lone Ross Graham Alan Ormondroyd Dimitrios KraniotisAbstract
Bio-based building materials offer a wide range of outlooks, from traditional rustic to modern design products. Recent development in the science of materials significantly improves their functional performance. However, when considering the use of bio-materials in outdoor environments, materials will deteriorate due to processes like weathering, oxidation, biodegradation, wear, and decay. Consequentially, biomaterials may lose visual appeal, leading to a perceived need for replacement even if the material is far from reaching functional failure. Visual assessment is the most direct method for evaluation of the aesthetic appearance of materials. However, it possesses a high degree of subjectivity when performed by an untrained person. On the contrary, measurement of surface properties with dedicated sensors provides objective values that might be related to the current state of the material in use. Recent developments in field of optics and electronics opens a new possibility to perform measurements in-situ. Colour-, gloss-, or spectro-photo-meters allow non-destructive measurements without particular sample preparation. Since all of the above techniques provide complementary information, the multi-sensor approach is more frequently suggested for applied research. The material state can be assessed regularly during service life. In this case, such measurement turns into monitoring. The paper illustrates examples of assessment and monitoring of biomaterials’ degradation due to weathering. Direct implementation of various sensors is demonstrated. A proposal for the approach of combining data provided by various sensing techniques with data mining is also presented.
Abstract
No abstract has been registered
Abstract
Mould growth is an important contributor to colour change of untreated wood exposed outdoors. Predicting the development of mould growth is therefore important to ensure successful use of untreated wood as a façade material. More knowledge about the factors affecting mould growth on outdoor exposed wood is required to give better predictions. In this study, climatic factors and material properties affecting mould growth have been investigated by exposing selected wooden specimens (aspen, pine sapwood, pine heartwood, spruce sapwood and spruce heartwood) to 8 different climates for 91 days. The climates were defined in a factorial design with two levels of relative humidity (65 and 85 %), wetting period (2 and 4 hours per day) and temperature (10 and 25 °C), respectively. The degree of mould growth was visually evaluated once a week during the exposure period. Aspen and pine sapwood were the substrates most susceptible to mould growth. There were no significant differences in susceptibility between pine heartwood and spruce heartwood, but the difference between heartwood and sapwood was significant for both pine and spruce. The effect of density on mould growth was tested for the spruce heartwood material, but was not found to reduce the residual variance significantly. However, all the tested climatic factors affected mould growth significantly; relative humidity was most important, while there was a somewhat smaller effect of wetting period and a minor effect of temperature. Overall, increased RH, longer wetting period and increased temperature had a positive effect on the mould growth. It was found a significant interaction between temperature and relative humidity, indicating that the temperature had larger effect on the mould growth at lower relative humidity, and that the relative humidity had larger effect at lower temperature. There was a tendency that the relative performance of the substrates was dependent on climate, but this interaction effect was not significant for any of the climatic factors.