Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2015
Abstract
Leaf blotch diseases in wheat can cause yield losses above 30 %. The necrotrophic fungus Parastagonospora nodorum is the dominating leaf blotch pathogen in Norwegian spring wheat. It has been well documented at the seedling stage that the pathogen produces necrotrophic effectors (NEs) which induces cell death in plants carrying susceptibility genes (Snn), allowing the necrotroph to enter. However, the role of these interactions under field conditions is less researched. In this study, we conducted field experiments with bi-parental and association mapping populations of spring wheat, to investigate the role of NE/Snn in adult plant resistance. The populations have been genotyped with the Illumina 90 K SNP chip, P. nodorum has high genetic diversity and both sexual and asexual reproduction, but the actual adaptation of the pathogen population to cultivars with different levels of resistance is not well studied. We are screening a collection of Norwegian isolates from known host sources to look for differences in NE-frequencies and haplotype distribution. The mapping populations are also inoculated and infiltrated with culture filtrates from single isolates on the seedling stage. Isolates involved in novel interactions will be deepsequenced in order to look for candidate effector genes. Potential effector proteins will be purified by LPC and HPLC to confirm their role in disease development.
Abstract
Leaf blotch diseases in wheat can cause yield losses above 30 %. The necrotrophic fungus Parastagonospora nodorum is the dominating leaf blotch pathogen in Norwegian spring wheat. It has been well documented at the seedling stage that the pathogen produces necrotrophic effectors (NEs) which induces cell death in plants carrying susceptibility genes (Snn), allowing the necrotroph to enter. However, the role of these interactions under field conditions is less researched. In this study, we conducted field experiments with bi-parental and association mapping populations of spring wheat, to investigate the role of NE/Snn in adult plant resistance. The populations have been genotyped with the Illumina 90 K SNP chip, P. nodorum has high genetic diversity and both sexual and asexual reproduction, but the actual adaptation of the pathogen population to cultivars with different levels of resistance is not well studied. We are screening a collection of Norwegian isolates from known host sources to look for differences in NE-frequencies and haplotype distribution. The mapping populations are also inoculated and infiltrated with culture filtrates from single isolates on the seedling stage. Isolates involved in novel interactions will be deepsequenced in order to look for candidate effector genes. Potential effector proteins will be purified by LPC and HPLC to confirm their role in disease development.
Authors
Matias Pasquali Marco Beyer Antonio Logrieco Kris Audenaert Virgilio Balmas Ryan Basler Anne-Laure Boutigny Jana Chrpova Elżbieta Czembor Tatiana Gagkaeva María Teresa González-Jaén Ingerd Skow Hofgaard Nagehan Desen Köycü Jelena Lević Patricia Marín García Thomas Miedaner Quirico Migheli Antonio Moretti Marina Elsa Herta Müller Françoise Munaut Päivi Parikka Marine Pallez Jonathan Scauflaire Barbara Scherm Slavica Stanković Ulf Thrane Silvio Uhlig Adriaan Vanheule Tapani Yli-Mattila Susanne VogelgsangAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Marco Ferretti Karin Hansen Vicent Calatayud Marta Camino-Serrano Nathalie Cools Bruno de Vos Tiina M. Nieminen Nenad Potocic Pasi Rautio Marcus Schaub Volkmar Timmermann Liisa Ukonmaanaho Peter WaldnerAbstract
No abstract has been registered
Authors
Marco Ferretti Tanja GM Sanders Alexa Michel Vicent Calatayud Nathalie Cools Elena Gottardini Matthias Haeni Karin Hansen Nenad Potocic Marcus Schaub Volkmar Timmermann Serina Trotzer Elena VanguelovaAbstract
No abstract has been registered
Authors
Walter Seidling Serina Trotzer Tanja GM Sanders Volkmar Timmermann Nenad Potocic Alexa MichelAbstract
No abstract has been registered
Abstract
No abstract has been registered
Abstract
No abstract has been registered
Abstract
Globally, billions of tons of carbon sequestered in trees are annually recycled back to the atmosphere through wood decomposition by microbes. In Norway, every fifth Norway spruce shows at final harvest infection by pathogenic white-rot fungi in the genera Heterobasidion and Armillaria. As these fungi can mineralize all components of wood, we predicted that they have a significant carbon footprint. Gas samples taken from infected stems were analyzed for CO2 and CH4 concentrations, and wood samples from different parts of the decay columns were incubated under hypoxic (4% O2) and anoxic laboratory conditions. In spring and summer the stem concentrations of CO2 were generally two times higher in trees with heartwood decay than in healthy trees. For most of the healthy trees and trees with heartwood decay, mean stem concentrations of CH4 were comparable to ambient air, and only some Armillaria infected trees showed moderately elevated CH4. Consistently, low CH4 production potentials were recorded in the laboratory experiment. Up-scaling of CO2 efflux due to wood decay in living trees suggests that the balance between carbon sequestration and emission may be substantially influenced in stands with high frequency of advanced root and stem heartwood decay.