Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2020
Abstract
Adjustable crop load primarily involves bud manipulation, and usually switches from vegetative to reproductive buds. While this switch is not fully understood, it is still controlled by the ratio of hormones, which promote or inhibit bud formation. To determine the reasons for biennial bearing, the effect of apple rootstock, scion cultivar, crop load, as well as metabolic changes of endogenous phytohormones [zeatin, jasmonic acid, indole-3 acetic acid (IAA), abscisic acid (ABA), and gibberellins 1, 3, and 7 (GAs)], and soluble sugars (glucose, fructose, and sorbitol) were evaluated, and their connections with return bloom and yield of apple tree buds were analyzed. Cultivars “Ligol” and “Auksis” were tested on five rootstocks contrasting in induced vigor: semi-dwarfing M.26; dwarfing M.9, B.396, and P 67; and super-dwarfing P 22. Crop load levels were adjusted before flowering, leaving 75, 113, and 150 fruits per tree. Principal component analysis (PCA) scatter plot of the metabolic response of phytohormones and sugars indicated that the effect of the semi-dwarfing M.26 rootstock was significantly different from that of the dwarfing M.9 and P 67, as well as the super-dwarfing P 22 rootstocks in both varieties. The most intensive crop load (150 fruits per tree) produced a significantly different response compared to less intensive crop loads (113 and 75) in both varieties. In contrast to soluble sugar accumulation, increased crop load resulted in an increased accumulation of phytohormones, except for ABA. Dwarfing rootstocks M.9, B.396, and P 67, as well as super-dwarf P 22 produced an altered accumulation of promoter phytohormones, while the more vigorous semi-dwarfing M.26 rootstock induced a higher content of glucose and inhibitory phytohormones, by increasing content of IAA, ABA, and GAs. The most significant decrease in return bloom resulted from the highest crop load in “Auksis” grafted on M.9 and P 22 rootstocks. Average difference in flower number between crop loads of 75 and 150 fruits per tree in “Ligol” was 68%, while this difference reached ~ 90% for P 22, and ~ 75% for M.9 and M.26 rootstocks. Return bloom was dependent on the previous year’s crop load, cultivar, and rootstock.
Authors
Aurita Butkeviciute Mindaugas Liaudanskas Darius Kviklys Dalia Gelvonauskiene Valdimaras JanulisAbstract
As the interest in heirloom cultivars of apple trees, their fruit, and processed products is growing worldwide, studies of the qualitative and quantitative composition of biological compounds are important for the evaluation of the quality and nutritional properties of the apples. Studies on the variations in the chemical composition of phenolic compounds characterized by a versatile biological effect are important when researching the genetic heritage of the heirloom cultivars in order to increase the cultivation of such cultivars in orchards. A variation in the qualitative and quantitative composition of phenolic compounds was found in apple samples of cultivars included in the Lithuanian collection of genetic resources. By the high-performance liquid chromatography (HPLC) method flavan-3-ols (procyanidin B1, procyanidin B2, procyanidin C2, (+)-catechin and (−)-epicatechin), flavonols (rutin, hyperoside, quercitrin, isoquercitrin, reynoutrin and avicularin), chlorogenic acids and phloridzin were identified and quantified in fruit samples of heirloom apple cultivars grown in Lithuania. The highest sum of the identified phenolic compounds (3.82 ± 0.53 mg/g) was found in apple fruit samples of the ‘Koštelė’ cultivar
Authors
Mindaugas Liaudanskas Rugile Okuleviciute Juozas Lanauskas Darius Kviklys Kristina Zymone Tamara Rendyuk Vaidotas Žvikas Nobertas Uselis Valdimaras JanulisAbstract
The aim of this study was to determine the composition and content of phenolic compounds in extracts of plum fruit. Fruit of 17 plum cultivars were analyzed. Fruit samples were collected in 2019 from fruit trees with “Myrobalan” (P. cerasifera Ehrh.) and “Wangenheim Prune” (P. domestica L.) rootstocks. The following glycosides of the flavonol group were identified: avicularin, isorhamnetin-3-O-rutinoside, isoquercitrin, hyperoside, rutin, and an aglycone quercetin. Compounds of the flavan-3-ol group were identified, such as (+)-catechin, procyanidin C1, and procyanidin A2, along with chlorogenic acid attributed to phenolic acids and a non-phenolic cyclitol–quinic acid. Of all the analytes identified in plum fruit samples, quinic acid predominated, while chlorogenic acid predominated among all the identified phenolic compounds, and rutin predominated in the flavonol group. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) revealed that fruit samples of “Kubanskaya Kometa”, “Zarechnaya Raniaya”, “Duke of Edinburgh”, “Jubileum”, and “Favorita del Sultano” cultivars had different quantitative content of phenolic compounds from that observed in other samples. The highest total amount of phenolic compounds was found in the European plum samples of the “Zarechnaya Rannyaya” cultivar, while the amount of quinic acid was the highest in plum fruit samples of the “Jubileum” cultivar.
Authors
Dainis Rungis Matti W. Leino Liga Lepse Smiljana Goreta Ban Erik de Vahl Külli Annamaa Priit Põldma Terhi Suojala-Ahlfors Danguole Juskeviciene Chris Kik Ingunn M. Vågen Helena StavelikovaAbstract
Potato onions (Allium cepa var aggregatum G. Don) are multiplying or aggregating onions, very similar to shallots and have been historically cultivated throughout Europe. Currently in Northern Europe they are maintained in home gardens and ex situ field collections. Potato onions are primarily vegetatively propagated, however in Estonia, near Lake Peipsi, this species has been propagated by seed since the seventeenth century. There is increasing interest in Northern Europe in utilizing this germplasm in organic and/or sustainable farming systems. The genetic diversity and relationship between and within European potato onion collections is unclear. From historical records it is known that cultivation, exchange and trade of potato onion has occurred throughout Europe for hundreds of years. This study utilised molecular markers to assess genetic diversity, duplication of genotypes and relationships among and between Nordic, Baltic, Czech and Croatian potato onion collections. Of 264 accessions, 80 catalogued as unique had identical genotypes with one or more other accessions, and are putative duplicates. The genetic diversity within two Estonian sexually propagated accessions was comparable to that found in all of the vegetatively propagated accessions. Accessions from the Nordic countries grouped together genetically, as did Latvian and Lithuanian accessions. Croatian accessions were genetically separated. These genetic relationships suggest historical movement of potato onion germplasm in North-Eastern Europe. The results, in conjunction with other passport and characterization data, can assist in the development of potato onion core collections, facilitating the conservation and utilization of valuable potato onion genetic resources.
Authors
Tomasz Leszek WoznickiAbstract
No abstract has been registered
Authors
Tomasz Leszek WoznickiAbstract
No abstract has been registered
Authors
Tomasz Leszek WoznickiAbstract
No abstract has been registered
Authors
Tomasz Leszek WoznickiAbstract
No abstract has been registered
Authors
Mekjell Meland Oddmund Frøynes Milica Fotiric Akšic Naris Pojskic Belma Kalamujic Stroil Lejla Lasic Fuad GasiAbstract
European plum cultivars (Prunus domestica L.) are hexaploid and partially self-fertile or self-sterile requiring compatible pollinizers with overlapping bloom times. Therefore, inter-planting of different pollinizer cultivars is recommended. In order to identify successful pollinizers of the plum cultivars ‘Edda’, ‘Opal’ (self-fertile), ‘Jubileum’, ‘Reeves’, ‘Mallard’, ‘Avalon’, ‘Cacanska Lepotica’ (self-fertile), and ‘Valor’, 60 fruits per cultivar were collected from nine orchards in 2017 and 2018, all of which were located in Ullensvang, western Norway. DNA extraction was subsequently conducted from the obtained embryos, followed by genetic characterization using seven microsatellite markers. Tissue samples from all possible pollinizers were collected during the summer of 2017 and the same DNA approach was conducted. Results showed that ‘Opal’ was the most successful pollinizer among the investigated plum cultivars. The main exception was ‘Cacanska Lepotica’, which consistently displayed very high level of self-pollination. The most successful foreign pollinizer of ‘Opal’ was ‘Mallard’. However, in more than two thirds of embryos extracted from ‘Opal’ fruits self-fertilization was determined. ‘Reeves’ was identified as the most successful pollinizer among embryos collected from ‘Valor’. Among the five cultivars (‘Edda’, ‘Jubileum’, ‘Reeves’, ‘Mallard’, and ‘Avalon’) that did not display self-pollination, the pollinizer success rate of ‘Opal’, ranged from 36.5% (‘Mallard’) to 93.5% (‘Edda’) in 2017, while in 2018 this rate ranged from 43.5% (‘Jubileum’ and ‘Reeves’) up to 96.5% (‘Edda’). Overall, genotyping embryos using SSRs (simple sequence repeats) proved an effective method in determining the success rate of individual pollinizers among European plum cultivars.
Abstract
In order to identify successful pollinizers of plum cultivars ‘Edda’ and ‘Opal’, 60 mature open pollinated fruit were collected from these two cultivars in autumn 2017. The fruits were harvested in three different orchards located in Ullensvang, the main fruit production area in Norway. After the harvest, kernels were separated from the fruit and used for the extraction of single embryos located within each kernel. At the same time, leaves were collected from the plum cultivars ‘Edda’ and ‘Opal’, as well as from all other plum cultivars present at the location and nearby orchards (‘Mallard’, ‘Jubileum’, ‘Reeves’, ‘Avalon’, ‘Valor’, ‘Cacanska Lepotica’ and ‘Herman’). DNA extraction was carried out for the embryos and leaves, and were used for genotyping using seven microsatellite markers. The microsatellite data were used for conducting paternity analyses based on a log likelihood ratio, where the fingerprint information from the leaves were the possible pollen donors available at the location. The results revealed within all examined orchards that the most successful pollinizer of ‘Edda’, a self-sterile cultivar, was ‘Opal’. The most successful foreign pollinizer of ‘Opal’ was ‘Mallard’. However, more than two thirds of embryos extracted from ‘Opal’ fruits did not present any alleles not already identified in ‘Opal’, which was expected considering that this plum cultivar was known to be self-fertile. European plums are hexaploid making its allele callings and paternity tests rather complicated. However, the approach used in this study gave clear answers regarding the most successful pollinizers.