Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2016
Abstract
No abstract has been registered
Authors
Jihong Liu ClarkeAbstract
No abstract has been registered
Authors
Jihong Liu ClarkeAbstract
No abstract has been registered
Authors
Johanna Gottschamel Andreas G. Lössl Stephanie Ruf Yanliang Wang Morten Skaugen Ralph Bock Jihong Liu ClarkeAbstract
Abstract Dengue fever is a disease in many parts of the tropics and subtropics and about half the world’s popula- tion is at risk of infection according to the World Health Organization. Dengue is caused by any of the four related dengue virus serotypes DEN-1, -2, -3 and -4, which are transmitted to people by Aedes aegypti mosquitoes. Cur- rently there is only one vaccine (DengvaxiaÒ) available (limited to a few countries) on the market since 2015 after half a century’s intensive efforts. Affordable and accessible vaccines against dengue are hence still urgently needed. The dengue envelop protein domain III (EDIII), which is capable of eliciting serotype-specific neutralizing antibod- ies, has become the focus for subunit vaccine development. To contribute to the development of an accessible and affordable dengue vaccine, in the current study we have used plant-based vaccine production systems to generate a dengue subunit vaccine candidate in tobacco. Chloroplast genome engineering was applied to express serotype- specific recombinant EDIII proteins in tobacco chloroplasts using both constitutive and ethanol-inducible expression systems. Expression of a tetravalent antigen fusion con- struct combining EDIII polypeptides from all four ser- otypes was also attempted. Transplastomic EDIII- expressing tobacco lines were obtained and homoplasmy was verified by Southern blot analysis. Northern blot analyses showed expression of EDIII antigen-encoding genes. EDIII protein accumulation levels varied for the different recombinant EDIII proteins and the different expression systems, and reached between 0.8 and 1.6 % of total cellular protein. Our study demonstrates the suitability of the chloroplast compartment as a production site for an EDIII-based vaccine candidate against dengue fever and presents a GatewayÒ plastid transformation vector for inducible transgene expression.
Authors
Xurong Mei Hongmin Dong Yue Li Nicholas Clarke Daozhi Gong Weiping Hao Yingchun Li Buchun Liu Xin Ma Wei XiongAbstract
No abstract has been registered
Authors
Mei Xurong Dong Hongmin Li Yue Nicholas Clarke Gong Daozhi Hao Weiping Li Yingchun Liu Buchun Ma Xin Xiong WeiAbstract
No abstract has been registered
Authors
Mei Xurong Dong Hongmin Li Yue Nicholas Clarke Gong Daozhi Hao Weiping Li Yingchun Liu Buchun Ma Xin Xiong WeiAbstract
No abstract has been registered
Authors
Nicholas ClarkeAbstract
No abstract has been registered
Authors
Nicholas ClarkeAbstract
No abstract has been registered
Authors
Nicholas ClarkeAbstract
No abstract has been registered