Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2023
Sammendrag
Currently global seaweed industries are facing issue with availability of raw material for processing of carrageenan due to low growth of current planting material. Use of biostimulants in seaweed cultivation is recently paid more attention due to their proven biostimulatory effect, of which, Ascophyllum marine plant extract powder (AMPEP) is a well proven biostimulant to improve the growth and quality of Kappaphycus alvarezii biomass. Hence, 500 kg of AMPEP was purchased and studied its impact on the commercial farming of K. alvarezii from April 2018 to January 2022 in India. Vegetative propagule of K. alvarezii were dipped in an AMPEP with concentration range of: 0.025, 0.05, 0.10, 0.15, 0.20 and 0.25 % for 30, 60, 90 and 120 min. Before out-planting on rafts in shallow coastal water and found that K. alvarezii responded well to a 0.1 % solution with dipping time of 60 min. The percentage of average daily growth rate (ADGR%) of AMPEP-treated plant in a 45 d grow out period was 3.50 ± 0.50 % vs a control of 1.75 ± 0.25 % for the summer and pre-monsoon months (p < 0.05) but no statistically significant differences between the treated and control plants were found during the rainy and winter seasons. Treated plants were found with low incidence of epiphytes, and disease as compared to control plants. The general appearance and health of treated Kappaphycus was good with significant differences in the yield and quality of semi-refined carrageenan (SRC) and dry weed quality (p < 0.05). The cost of AMPEP for producing of additional 1 kg of dry Kappaphycus was 0.066USD. Results of the present study is very encouraging and AMPEP can be used for the production of K. alvarezii biomass for industrial and biorefinery processing as it has been witnessed that there was 16.66 % increase in biomass production in 2021in India.
Forfattere
Thilaga Sethuraman Shanmugam Munisamy Ganesan Meenakshisundaram Anicia Q. Hurtado Ganesh DossSammendrag
Regeneration of polyploidy from young thallus segments of Kappaphycus alvarezii was optimized for genetic improvement. Kappaphycus thallus segment cultured on sterile sea water supplemented with various combinations of Indole acetic acid, Kinetin and Acardian Marine Plant Extract Powder revealed differential response on callus proliferation and development of new thallus. Presence of Acardian Marine Plant Extract Powder (3 mg/l) in combination with Indole acetic acid and Kinetin (0.01 mg/l each) had induced the longest emerging thallus. Exposure of thallus to colchicine at 0.01% with above combination was optimal to induce high frequency regeneration of polyploidy mostly from the meristematic cells. Anatomical study of colchicine induced polyploidy revealed larger cortical cells with irregular thickening of epidermal layer. Phase contrast and Scanning Electron Microscopic study revealed increase in cell size in cortical region with significantly larger number of spherical shaped carrageenan globules in colchicine induced polyploidy than normal thallus. Single cells isolated using enzymatic treatments from colchicine induced polyploidy, shown chromosome number with a ploidy status of 4n ≈ 40. Whereas in normal thallus, only half the number of chromosomes (2n ≈ 20) were observed. Polyploidy were successfully acclimatized gradually using raft method for further evaluation. This is the first report reveals the induction and regeneration of polyploidy in Kappaphycus. The possible application of this finding in genetic improvement of Kappaphycus is discussed.
Forfattere
Aswini Murugan Thahira Banu Shanthana Lakshmi Duraikkannu Shanmugam Munisamy Kiruthigha Vannamuthu Subhalakshmi SivakumarSammendrag
The present investigation is aimed at developing films using biomass of seaweed Kappaphycus alvarezii blended with natural additives both without glycerol (K) and with glycerol (KG) and to use the same in enhancement of shelf life of tomatoes. The natural additives used were Azadirachta indica oil (K1), A. indica leaves extract (K2), Citrus limon leaves extract (K3), A. indica gum (K4), Prunus amygdalus gum (K5), Moringa indica gum (K6) and the extract and oil of A. indica leaves (K7). The result showed that the moisture content ranged from 10.3 ± 1.2–19.9 ± 0.4%, thickness 0.01–0.10 mm, and swelling index above 45% in all the prepared films. The tensile strength and transparency were higher for K6, KG6, K and KG respectively. KG6 and K3 films showed maximum phenolic content, antioxidant and antimicrobial activity. The promising results of current investigation proved the suitability of the film in enhancing the shelf life of tomatoes to 14 days at room temperature.
Forfattere
Bhagirath Rakhasiya Shanmugam Munisamy Doniya Elze Mathew Srinu Tothadi Anshul Yadav Vaibhav A. MantriSammendrag
Processing of 1000 kg of fresh Kappaphycus alvarezii yielded 25 kg salt. The optical microscopy revealed non-crystalline nature, absence of geometrical pattern; while squircle aggregates with ruffled surface were evident in SEM. Caenorhabditis elegans assay confirmed its non-toxicity; while absence of Salmonella and pesticides make it safe for human consumption. Among the macro elements, ‘K' content was highest (3792.74 ± 541.23 mg 100 g−1) and ‘Ca’ was lowest (7.01 ± 1.32 mg 100 g−1); whereas among micro element ‘Fe’ was highest (6.52 ± 1.40 mg 100 g−1) and ‘Ni’ lowest (0.14 ± 0.01 mg 100 g−1). Chromium (11.5 μg g−1) was lower than a permissible daily dose, while other heavy metals (Mo, Cu, Co, Zn, As, Cd, Hg, and Pb) were below detectable level. PXRD revealed that it has only KCl Form I. The study suggested potential economic utility of seaweed salt under the framework of waste to wealth.
Forfattere
Gabriella do Vale Pereira Luis E. C. Conceição Filipe Soares Jessica Petereit Bela H. Buck Johan Johansen Jorge Dias Filippo FaccendaSammendrag
This trial aimed to assess the growth performance of trout (Oncorhynchus mykiss) fed novel formulations, evaluate fish welfare status, and determine flesh quality as part of the evaluation of sustainable feeds. A control diet containing fish meal and soy products (CTRL) was compared to: a diet with processed animal proteins (PAP); a diet without PAP (NoPAP); a PAP diet lower in protein (PAP−); and a NoPAP diet higher in protein (NoPAP+). Groups of 50 fish, weighing 58.84 ± 1.39 g (IBW), were allocated to 20 tanks and fed with formulated diets ad libitum over 91 days. Better growth performance was observed after the experiment in fish fed the NoPAP+ diet when compared to other diets. Protein retention was higher in CTRL diets than in PAP and PAP− diets. Protein and phosphorous digestibility were lower in fish fed PAP− diet. Diets did not influence the texture analysis. However, sensory analysis revealed higher acceptance for fish fed the NoPAP diet when compared to the PAP diet. Lysozyme was higher in the NoPAP diet than in other treatments. In addition, long-term predictions using FEEDNETICSTM software suggest some of these alternative formulations may be economically sustainable. Overall, these results support the hypothesis that the new formulations are viable options for trout farming.
Forfattere
Espen Rimstad Dean Basic Åsa Maria Olofsdotter Espmark Thomas Fraser Snorre Gulla Johan Johansen Tor Atle Mo Ingrid Olesen Rolf-Erik Olsen Erik Georg Bø-Granquist Sokratis Ptochos Amin Sayyari Bjørnar YtrehusSammendrag
Background and terms of reference Farmed Atlantic salmon (Salmo salar) that escape into the wild could interbreed with native fish, posing a potential risk to the genetic diversity of wild Atlantic salmon populations. The Atlantic salmon in aquaculture are diploid, meaning the fish has two sets of chromosomes. To mitigate the genetic impact on wild populations, the concept of producing sterile triploid farmed Atlantic salmon has been suggested as a solution. However, it is important to ensure that the utilization of triploids in commercial farming aligns with the regulations set forth in the Norwegian Animal Welfare Act. The Norwegian Food Safety Authority (NFSA) requested the Norwegian Scientific Committee for Food and Environment (VKM) to do an assessment about health- and welfare consequences in triploid Atlantic salmon under commercial farming conditions, as compared to diploid counterparts. VKM was also requested to describe the underlying physiological mechanisms concerning consequences of triploidy as well as address potential measures to reduce the negative impacts on the health and welfare of the fish. Methods A working group consisting of members with expertise in salmonid biology, aquaculture systems, veterinary medicine, fish health and welfare, virology, bacteriology, parasitology, breeding and genetics has drafted this opinion. To answer the Terms of Reference as mandated by the NFSA, the authors addressed fish health and welfare as a unified concept in this report. Two external experts have reviewed and provided their opinion before it was assessed and approved by the VKM’s Panel on Animal Health and welfare. The literature used in this work was peer-reviewed studies retrieved from a search in four databases as well as non peer-reviewed reports. Selection of studies was conducted independently by two members in the working group and based on predefined inclusion and exclusion criteria. Conclusions Under commercial farming conditions, triploid Atlantic salmon are often found to have lower standards of health and welfare compared to diploids. For example, field and experimental studies have found triploids to be more prone to skeletal and heart deformities, and cataracts, while field studies suggest that under commercial farming conditions they cope less well with handling and are more susceptible to skin ulcers. However, research has indicated that some of the effects of triploidy can be mitigated through specialized diets or environmental adjustments. There is a noticeable tendency across farm studies and experimental trials for triploid salmon to be equal or larger in size at the end of freshwater phase, but equal or smaller in size at the end of the seawater phase. Most publications conclude that within what is considered the optimal temperature range of diploids, oxygen consumption rate, oxygen binding capacity, and aerobic swimming capacity do not significantly differ between triploid and diploid Atlantic salmon. However, findings from experimental trials suggest a lower optimal temperature range for triploids, and data consistent across studies indicate that triploids possess lower tolerance to hypoxia at elevated temperatures. Triploid Atlantic salmon are less robust to higher water temperatures than diploid, and have other nutritional needs than diploids, especially regarding phosphorus, and histidine. There are few studies on the susceptibility of triploid salmon to infectious agents and diseases. Field observations indicate that triploid fish are more susceptible to primary infectious salmon anaemia (ISA) outbreaks than diploids under commercial farming conditions at the level of the farm, and at cage level within farms that experience an ISA outbreak. A higher susceptibility to the ISA virus would potentially affect not only the health and welfare of the triploid fish at the farm with an outbreak but may potentially spread to other farms. .............
Forfattere
Ralf RautenbergerSammendrag
Aquaculture of marine macroalgae is an important part of the world’s food production. In Norway, the fast-growing kelp Saccharina latissima has the highest potential for industrial biomass production. Aquaculture in the country’s fjords is economically more viable for SMEs, supports the development of IMTA and could allow the industry to approach the projected 20 million tons by 2050. However, S. latissima is exposed to a considerable decline in seawater salinity during the growth season, which affects the biomass production. This presentation shows results of industrial R&D projects in which the presence of “low-salinity tolerant” strains of S. latissima in a North Norwegian fjord and their responses to the seasonal salinity decline was studied. In a laboratory-based common garden experiment, sporophytes of S. latissima from different locations in Skjerstadfjorden were cultivated under different salinities for six weeks. Growth and photosynthetic parameters were measured to understand their physiological responses to salinity stress. Then their F1 generation were seeded on ropes and deployed at a commercial aquaculture site in Skjerstadfjorden to study strain-specific differences in biomass production and yield, optimal growth depths and biochemical composition of S. latissima. In addition, the aquaculture site was characterised by measurements of physical and chemical parameters. The projects’ results will help North Norwegian macroalgae producers to improve the biomass production and biochemical composition of S. latissima. These findings could lay the foundation for the development of breeding programmes in Norway and could demonstrate the macroalgae producers in Norway the possibility of establishing aquaculture in fjords.
Sammendrag
Global measures to bring net-zero-carbon and zero-waste emissions are expanding at a rapid pace. Currently, only 16% of the plastic waste from the food industrial sector is reprocessed and recycled, which is way lesser than its accumulation. Several countries have imposed a ban on single-use plastic derived from food and/or beverage industries. All these constraints and challenges have encouraged researchers to find a sustainable alternative to petroleum-based food packaging. The environmentally friendly substitute can be the bio-based polymer material derived from agri-food and marine wastes that connect the waste loop in the current economic model. This waste has the most valuable biopolymer mainly present in the cell wall matrix of plants, animals, bacteria, fungi, and algae. All these biopolymers are either accumulated in a landfill or not entirely harvested their high-value compounds as a potential feedstock. Nevertheless, bio-based polymers have better thermos-mechanical properties that can resist various conditions. They comprise superior functional properties when these biopolymers are coupled with other organic compounds such as composite films or multilayer packaging films which enhance the shelf-life of the food. Overall, biopolymers readily react with the soil microbes under specified environmental conditions that can significantly enhance the biodegradability of packaging material. This unique quality is envisaged to solve the existing problems and detrimental effects of synthetic polymer usage in the food industry. In this background, in this chapter, the origin of biopolymers and their potential functionality, mechanical property, and degradability as food packaging materials are discussed. Their current challenges and possible future prospects are also meticulously highlighted.
Forfattere
Kannan Mohan Palanivel Sathishkumar Durairaj Karthick Rajan Jayakumar Rajarajeswaran Abirami Ramu GanesanSammendrag
Black soldier fly larvae (BSFL) Hermetia illucens is fastest growing and most promising insect species especially recommended to bring high-fat content as 5th generation bioenergy. The fat content can be fully optimized during the life-cycle of the BSFL through various organic dietary supplements and environmental conditions. Enriched fat can be obtained during the larval stages of the BSF. The presence of high saturated and unsaturated fatty acids in their body helps to produce 70 % of extractable oil which can be converted into biodiesel through transesterification. The first-generation biodiesel process mainly depends on catalytic transesterification, however, BSFL had 94 % of biodiesel production through non-catalytic transesterification. This increases the sustainability of producing biodiesel with less energy input in the process line. Other carbon emitting factors involved in the rearing of BSFL are less than the other biodiesel feedstocks including microalgae, cooking oil, and non-edible oil. Therefore, this review is focused on evaluating the optimum dietary source to produce fatty acid rich larvae and larval growth to accumulate C16–18 fatty acids in larger amounts from agro food waste. The process of optimization and biorefining of lipids using novel techniques have been discussed herein. The sustainability impact was evaluated from the cultivation to biodiesel conversion with greenhouse gas emissions scores in the entire life-cycle of process flow. The state-of-the-art in connecting circular bioeconomy loop in the search for bioenergy was meticulously covered.
Sammendrag
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